CN-121974502-A - Anaerobic ammonia oxidation sludge granule culture method, device and application

CN121974502ACN 121974502 ACN121974502 ACN 121974502ACN-121974502-A

Abstract

The invention provides a culture method, a device and application of anaerobic ammonia oxidation sludge particles, and belongs to the technical field of anaerobic ammonia oxidation. The preparation method of the iron-rich activated carbon comprises the steps of taking the iron-rich activated carbon as a carrier, inoculating sludge, adding nutrient solution to carry out sludge granulation culture, soaking the activated carbon in potassium ferrate solution, drying to obtain potassium ferrate modified activated carbon, and calcining the potassium ferrate modified activated carbon in an anaerobic environment to obtain the iron-rich activated carbon, wherein the sludge contains Bacteroidota, planctomycetota and Chloroflexi microbial flora. According to the invention, iron-rich activated carbon is used as a carrier, EPS with high content ratio of AL-2 and c-di-GMP bi-component quorum sensing signal molecules and other proteins secreted by flora is utilized, and copolymerization of the three bacteria is promoted by virtue of Chloroflexi filiform structures and sliding movement capability, so that granulation of important family members Planctomycetota serving as anaerobic ammonia oxidizing bacteria is promoted.

Inventors

PEI JIANCHUAN
YANG JINYAN
ZHENG YANSHI
SHEN JIARONG
FENG HUAJUN
LIANG JIAYU

Assignees

浙江农林大学

Dates

Publication Date: 20260505
Application Date: 20260331

Claims (10)

1. A method for culturing anaerobic ammoxidation sludge particles is characterized by comprising the following steps of taking iron-rich activated carbon as a carrier, inoculating sludge, and adding nutrient solution to perform sludge granulation culture; the preparation method of the iron-rich activated carbon comprises the steps of impregnating the activated carbon with potassium ferrate solution, drying to obtain potassium ferrate modified activated carbon, calcining the potassium ferrate modified activated carbon in an anaerobic environment to obtain the iron-rich activated carbon; The sludge contains Bacteroidota, planctomycetota and Chloroflexi microbial flora.
2. The culture method according to claim 1, wherein the sludge is surplus sludge in a landfill leachate denitrification tank, and the inoculation concentration is 5-15 g/L.
3. The culture method of claim 1, wherein the initial formulation of the nutrient solution comprises 20-50 mg/L of ammonium chloride, 20-50 mg/L of sodium nitrite, 20-50 mg/L of monopotassium phosphate, 100-500 mg/L of magnesium sulfate heptahydrate, 50-300 mg/L of calcium chloride dihydrate and 0-20 mg/L of potassium bicarbonate.
4. The method according to claim 3, wherein the concentration of ammonium chloride and sodium nitrite in the nutrient solution is increased by 10-50 mg/L in a concentration gradient until the maximum concentration is 400mg/L, and the concentration of potassium bicarbonate is increased by 20-200 mg/L in a concentration gradient until the maximum concentration is 1250mg/L, each time the ammonia nitrogen removal rate is > 90%.
5. The method according to claim 3, wherein the nutrient solution has a flow rate of 1 to 5mL/min, DO <0.5mg/L, and a temperature of 25 to 40 ℃.
6. The method according to claim 1, wherein the sludge granulation culture is completed when Bacteroidota is >20%, planctomycetota is >15%, and Chloroflexi is >25% of the microbial flora of the sludge granules.
7. The anaerobic ammonium oxidation sludge granules produced by the culture method according to any one of claims 1 to 6.
8. Use of the anaerobic ammonium oxidation sludge granules of claim 7 in sewage treatment.
9. A sludge granulation device used in the cultivation method according to any one of claims 1 to 6, comprising a nutrient solution pretreatment system and a sludge granulation cultivation system arranged in a thermostatic chamber; The nutrient solution pretreatment system comprises a temporary storage and a gas cylinder, wherein the top of the temporary storage is provided with a nutrient solution inlet pipe, and a submersible pump is arranged in the temporary storage; The system comprises an air chamber, a granulating chamber and a granular sludge temporary storage chamber which are sequentially communicated from top to bottom, wherein the top of the air chamber is provided with a pressure sensor and an exhaust pipe, and the bottom of the air chamber is provided with an air passing hole communicated with the granulating chamber; The device also comprises an activated carbon adding pipe which passes through the air chamber and the air passing holes from top to bottom and stretches into the granulating chamber, and an upward floating sludge stopper is arranged at the pipe orifice end in the granulating chamber.
10. The sludge granulation device according to claim 9, wherein electromagnetic valves are provided on the nutrient solution inlet pipe, the air pipe, the input pipe, the exhaust pipe and the sludge discharge pipe, and liquid level meters are provided in the temporary storage and the granulation chamber.

Description

Anaerobic ammonia oxidation sludge granule culture method, device and application Technical Field The invention belongs to the technical field of anaerobic ammonia oxidation, and particularly relates to a culture method, a device and application of anaerobic ammonia oxidation sludge particles. Background The anaerobic ammonia oxidation process has extremely low energy consumption, does not need to supply nutrients, does not need to add extra carbon sources, has low carbon emission, and is a green low-carbon sewage denitrification process. However, because the growth rate of anaerobic ammonia oxidation bacteria is slow, the yield of Extracellular Polymer (EPS) serving as a natural microbial binder is low, the Extracellular Polymer (EPS) is difficult to effectively bind, and nitrogen generated in the metabolic process can cause certain disturbance to the external environment in the escaping process, so that the binding force among bacteria is destroyed, the stability of granular sludge is influenced, bacterial loss is easy to cause, the starting failure rate of an anaerobic ammonia oxidation process is high, and the starting period is long. If the EPS quantity can be increased, the sludge granulation is promoted, the loss of anaerobic ammonia oxidizing bacteria can be effectively reduced, the process starting success rate can be increased, and the starting time is shortened. The existing methods for promoting the granulation of the anaerobic ammonia oxidation sludge mainly comprise two methods, namely a method for accelerating the formation of the granular sludge by adding acyl homoserine lactone, and a method for promoting the formation of the granular sludge by taking effluent of an anaerobic ammonia oxidation granular sludge reactor containing various quorum sensing signals as water distribution by adopting the method as in the application patent CN 117865342A. However, the former has higher external feeding cost, the latter still needs anaerobic ammonia oxidation sludge fermentation to produce after water distribution, and the content of the ferment is easily influenced by process fluctuation and is not easy to control, and raw materials are needed to be added, the occupied area is increased by 2 series-connected devices, the whole starting time is longer, and the anaerobic ammonia oxidation bacteria standard and large-scale granulation culture are not facilitated. Disclosure of Invention In view of the above, the invention aims to provide a culture method, a device and application of anaerobic ammonia oxidation sludge granules, which are used for solving the problems of difficult granulation, long starting period and poor running stability of anaerobic ammonia oxidation bacteria. The invention realizes the efficient and rapid granulation of anaerobic ammonia oxidation sludge and forms stable granular sludge with a specific functional flora structure. In order to achieve the above object, the present invention provides the following technical solutions: A method for culturing anaerobic ammoxidation sludge particles comprises inoculating sludge with iron-rich activated carbon as carrier, and adding nutrient solution to perform sludge granulation culture; the preparation method of the iron-rich activated carbon comprises the steps of impregnating the activated carbon with potassium ferrate solution, drying to obtain potassium ferrate modified activated carbon, calcining the potassium ferrate modified activated carbon in an anaerobic environment to obtain the iron-rich activated carbon; The sludge contains Bacteroidota, planctomycetota and Chloroflexi microbial flora. Preferably, the sludge is surplus sludge in a landfill leachate denitrification tank, and the inoculation concentration is 5-15 g/L. Preferably, the initial formula of the nutrient solution comprises 20-50 mg/L of ammonium chloride, 20-50 mg/L of sodium nitrite, 20-50 mg/L of monopotassium phosphate, 100-500 mg/L of magnesium sulfate heptahydrate, 50-300 mg/L of calcium chloride dihydrate and 0-20 mg/L of potassium bicarbonate. More preferably, in the culture stage, each time the ammonia nitrogen removal rate is more than 90%, the concentration gradient of the ammonium chloride and the sodium nitrite in the nutrient solution is increased by 10-50 mg/L until the maximum concentration is 400mg/L, and the concentration gradient of the potassium bicarbonate is increased by 20-200 mg/L until the maximum concentration is 1250mg/L. More preferably, in the culture stage, the flow rate of the nutrient solution is 1-5 mL/min, DO is less than 0.5mg/L, and the temperature is 25-40 ℃. Preferably, the sludge granulation culture is completed when Bacteroidota is >20%, planctomycetota is >15% and Chloroflexi is >25% of the microbial flora of the sludge granules. The invention also provides anaerobic ammonia oxidation sludge particles prepared by the culture method and application thereof in sewage treatment. The invention also provides a sludge granulating dev