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CN-121974749-A - Composite microbial functional fertilizer, and preparation method and application thereof

CN121974749ACN 121974749 ACN121974749 ACN 121974749ACN-121974749-A

Abstract

The invention discloses a compound microorganism functional fertilizer, a preparation method and application thereof, and belongs to the technical field of microorganism fertilizers. The functional fertilizer is prepared by taking 6 functional strains such as clostridium butyricum (CCTCC NO: M2022151), bacillus coagulans (CGMCC NO. 32216), kluyveromyces marxianus (CGMCC NO. 24455) and the like as cores and matching with auxiliary materials such as soybean concentrated protein and the like through a two-stage composite fermentation process. Experiments show that the functional fertilizer can remarkably improve the contents of trace elements in quick-acting phosphorus, potassium, calcium, magnesium, iron, zinc and the like in soil, effectively repair the degraded soil structure, remarkably improve the mu yield of wheat, the wheat protein content and the wet gluten content when being used for wheat planting, and greatly improve the crop quality. The invention has the double effects of soil restoration and crop yield and quality improvement, and provides a new technical scheme for sustainable development of green agriculture.

Inventors

  • HAN ZHIPENG
  • LI KEKE
  • GUO SONGFENG
  • FENG LIFEI
  • ZHU MENGJIE
  • Du hanxiao

Assignees

  • 河南金百合生物科技股份有限公司

Dates

Publication Date
20260505
Application Date
20260206

Claims (10)

  1. 1. The preparation method of the compound microorganism functional fertilizer is characterized by comprising the following steps: (1) Fermenting clostridium butyricum seed liquid with the preservation number of M2022151 by using an improved CM culture medium, wherein the temperature is 34-36 ℃, the fermentation time is 34-36 hours, and centrifuging the fermentation liquid to obtain clostridium butyricum fermentation supernatant, the composition of the improved CM culture medium is 1.3-1.6 g glucose, 0.4-0.6 g corn starch, 2.5-3.5 g peptone and the balance of yeast powder 2~3g,K 2 HPO 4 0.06~0.09g,MgSO 4 •7H 2 O 0.04~0.06g,MnSO 4 •H 2 O 0.004~0.006g,; (2) Culturing bacillus coagulans seed liquid with a preservation number of CGMCC No.32216 by using an MRS liquid culture medium, wherein the temperature is 36-38 ℃, the fermentation time is 22-26 h, culturing Kluyveromyces marxianus seed liquid with a preservation number of CGMCC No.24455 by using an expansion culture medium, the temperature is 29-31 ℃, the fermentation time is 46-50 h, simultaneously culturing lactobacillus plantarum and lactobacillus casei seed liquid by using a culture medium, the temperature is 29-31 ℃, the fermentation time is 46-50 h, the culture medium comprises 9-11.0 g of peptone, 3-5.0 g of yeast extract powder, 0.8-1.2 g of tween 80, 1-3.0 g of dipotassium phosphate heptahydrate, 4-6.0 g of sodium acetate trihydrate, 1-3.0 g of ammonium citrate, 0.1-0.3 g of magnesium sulfate tetrahydrate, 0.04-0.06 g of manganese sulfate tetrahydrate, 9-11.0 g of maltose, 0.004-0.0.005 g of bromopurple, 2.2 g of bacillus subtilis, the fermentation time is 2-50 h, the culture medium comprises 9-11.0 g of peptone, 3-2 g of saccharose, the culture medium is 3-2 g of glucose extract, the fermentation time is 1-2 mL, and the culture medium is 1-50 h, the culture medium is obtained by using the expansion culture medium, and the culture medium has a pH value of 3-2 mL of 3; (3) Mixing 35-45 Kg of soybean concentrated protein powder, 70-90 g of alkaline protease, 300-340L of clostridium butyricum fermentation supernatant with 45-55 mL of bacillus coagulans fermentation liquid and 45-55 mL of bacillus subtilis fermentation liquid, and performing primary fermentation at 54-56 ℃ for 14-18 hours; (4) After the primary fermentation is finished, adding 3-4 kg of molasses, 1-3 kg of glucose, 0.7-0.9 kg of dipotassium hydrogen phosphate, 1.3-1.7 kg of light calcium carbonate, 0.7-0.9 kg of puffed urea and 26-30 mL of Kluyveromyces marxianus fermentation liquor for secondary fermentation, wherein the temperature is 29-31 ℃ and the fermentation time is 22-26 hours; (5) And after the secondary fermentation is finished, adding 90-110L of water, 0.8-1.2 kg of molasses, 60-70 mL of mixed fermentation liquid of lactobacillus plantarum and lactobacillus casei, and uniformly stirring to obtain the compound microorganism functional fertilizer.
  2. 2. The method according to claim 1, wherein the modified CM medium in step (1) comprises 1.5g of glucose, 0.5g of corn starch, 3g of peptone, 3g of yeast powder, 0.005g of K 2 HPO 4 0.08g,MgSO 4 •7H 2 O 0.05g,MnSO 4 •H 2 O and the balance of water.
  3. 3. The preparation method according to claim 2, wherein the culture medium in the step (2) comprises 10.0g of peptone, 4.0g of yeast extract powder, 1.0g of tween 80, 2.0g of dipotassium hydrogen phosphate heptahydrate, 5.0g of sodium acetate trihydrate, 2.0g of triammonium citrate, 0.2g of magnesium sulfate heptahydrate, 0.05g of manganese sulfate tetrahydrate, 10.0g of maltose and 0.0048g of bromocresol purple.
  4. 4. The method according to claim 3, wherein the composition of the medium for the expansion in the step (2) is composed of 2g of glucose, 2g of peptone, 1g of yeast extract and 100mL of purified water.
  5. 5. The method according to claim 4, wherein the amount of the soybean protein concentrate, alkaline protease, clostridium butyricum fermentation supernatant, bacillus coagulans fermentation broth and bacillus subtilis fermentation broth in the step (3) is 40Kg, 80g, 320L, 50mL and 50mL, respectively.
  6. 6. The process according to claim 5, wherein the fermentation temperature in the step (3) is 55℃and the fermentation time is 16 hours.
  7. 7. The method according to claim 6, wherein the amount of molasses, glucose, dipotassium hydrogen phosphate, light calcium carbonate, puffed urea and Kluyveromyces marxianus fermentation liquid used in the step (4) is 3.5kg, 2kg, 0.8kg, 1.5kg, 0.8kg and 28mL respectively.
  8. 8. The method according to claim 7, wherein the amounts of the mixed fermentation liquid of water, molasses, lactobacillus plantarum and lactobacillus casei in the step (5) are 100L, 1kg and 65mL respectively.
  9. 9. A composite microbial functional fertilizer produced by the production method of any one of claims 1 to 8.
  10. 10. The use of the composite microbial functional fertilizer of claim 9 for restoring degraded soil and improving crop yield and quality.

Description

Composite microbial functional fertilizer, and preparation method and application thereof Technical Field The invention belongs to the technical field of microbial fertilizers, and particularly relates to a compound microbial functional fertilizer, a preparation method and application thereof. Background At present, a large amount of chemical fertilizer products are used in agricultural cultivation, on one hand, the crop yield is improved, on the other hand, the environmental pollution, especially the pollution of soil environment, is aggravated, the soil pollution is difficult to repair in a short period, and a large amount of element fertilizers are applied, so that chemical substances such as nitrogen, phosphorus, potassium and the like are easy to be solidified by soil, and various chemical salts are formed to accumulate in the soil. This not only damages the aggregate structure of the soil, but also causes imbalance of the soil nutrient structure. The chemical fertilizer is used in a large amount for a long time, so that the content of harmful metals and germs in the soil exceeds the standard, the physical properties are poor, and finally the soil fertility is reduced, and the growth of crops is affected. The chemical fertilizer has single general components, and the nutrients in the soil tend to be single after long-term use. The crops not only need nitrogen, phosphorus and potassium, but also need many microelements such as calcium, iron, zinc and selenium, and the single nutrient can not meet the growth requirement of the crops. Therefore, melons and fruits become not sweet, and vegetables are not fragrant. Excessive use of chemical fertilizer also makes the fruit and vegetable growth character inferior, easy to rot and not suitable for storage. Beneficial bacteria in the microbial fertilizer accelerate the decomposition of soil organic matters and promote the formation of aggregate structures, thereby breaking the soil hardening, and regulating and improving the water retention, fertilizer retention and air permeability of the soil, so that the microbial fertilizer is more suitable for crop growth. Meanwhile, pesticide heavy metal residues in the soil are degraded, and the polluted soil environment is restored. Disclosure of Invention In order to solve the technical problems, the invention provides a preparation method of a compound microorganism functional fertilizer, which comprises the following steps: (1) Fermenting clostridium butyricum seed liquid with the preservation number of M2022151 by using an improved CM culture medium, wherein the temperature is 34-36 ℃, the fermentation time is 34-36 hours, and centrifuging the fermentation liquid to obtain clostridium butyricum fermentation supernatant, the composition of the improved CM culture medium is 1.3-1.6 g glucose, 0.4-0.6 g corn starch, 2.5-3.5 g peptone and the balance of yeast powder 2~3g,K2HPO4 0.06~0.09g,MgSO4•7H2O 0.04~0.06g,MnSO4•H2O 0.004~0.006g,; (2) Culturing bacillus coagulans seed liquid with a preservation number of CGMCC No.32216 by using an MRS liquid culture medium, wherein the temperature is 36-38 ℃, the fermentation time is 22-26 h, culturing Kluyveromyces marxianus seed liquid with a preservation number of CGMCC No.24455 by using an expansion culture medium, the temperature is 29-31 ℃, the fermentation time is 46-50 h, simultaneously culturing lactobacillus plantarum and lactobacillus casei seed liquid by using a culture medium, the temperature is 29-31 ℃, the fermentation time is 46-50 h, the culture medium comprises 9-11.0 g of peptone, 3-5.0 g of yeast extract powder, 0.8-1.2 g of tween 80, 1-3.0 g of dipotassium phosphate heptahydrate, 4-6.0 g of sodium acetate trihydrate, 1-3.0 g of ammonium citrate, 0.1-0.3 g of magnesium sulfate tetrahydrate, 0.04-0.06 g of manganese sulfate tetrahydrate, 9-11.0 g of maltose, 0.004-0.0.005 g of bromopurple, 2.2 g of bacillus subtilis, the fermentation time is 2-50 h, the culture medium comprises 9-11.0 g of peptone, 3-2 g of saccharose, the culture medium is 3-2 g of glucose extract, the fermentation time is 1-2 mL, and the culture medium is 1-50 h, the culture medium is obtained by using the expansion culture medium, and the culture medium has a pH value of 3-2 mL of 3; (3) Mixing 35-45 Kg of soybean concentrated protein powder, 70-90 g of alkaline protease, 300-340L of clostridium butyricum fermentation supernatant with 45-55 mL of bacillus coagulans fermentation liquid and 45-55 mL of bacillus subtilis fermentation liquid, and performing primary fermentation at 54-56 ℃ for 14-18 hours; (4) After the primary fermentation is finished, adding 3-4 kg of molasses, 1-3 kg of glucose, 0.7-0.9 kg of dipotassium hydrogen phosphate, 1.3-1.7 kg of light calcium carbonate, 0.7-0.9 kg of puffed urea and 26-30 mL of Kluyveromyces marxianus fermentation liquor for secondary fermentation, wherein the temperature is 29-31 ℃ and the fermentation time is 22-26 hours; (5) And after the secondary fe