CN-121974960-A - Environment-friendly method for extracting persimmon polyphenol from persimmons

Abstract

The invention relates to the technical field of persimmon polyphenol extraction, and provides a green environment-friendly method for extracting persimmon polyphenol from persimmons, which comprises the following steps of S1, taking persimmons as raw materials, washing, drying, crushing, adding glacial acetic acid aqueous solution, adjusting pH to 4-5, adding enzyme, mixing, and performing enzymolysis to obtain mixed liquor I, S2, extracting the mixed liquor I with alcohol, centrifuging, taking a liquid part to obtain mixed liquor II, S3, adsorbing the mixed liquor II with resin, adding an organic solvent, eluting, obtaining eluent, concentrating, and drying to obtain persimmon polyphenol, wherein in the step S2, the enzymolysis comprises first enzymolysis and second enzymolysis, the enzymes adopted by the first enzymolysis are different from the enzymes adopted by the second enzymolysis, the enzymes adopted by the first enzymolysis are pectase, and the enzymes adopted by the second enzymolysis are composed of hemicellulase and beta-glucosidase. Through the technical scheme, the problem of insufficient yield and purity of persimmon polyphenol extraction in the related technology is solved.

Inventors

• Zheng jingmei
• LI WEIJIA
• SHAO DEMIN
• MA CHAO
• ZHANG YIMENG
• YANG MINGYU

Assignees

• 河北瑞龙生物科技有限公司

Dates

Publication Date

20260505

Application Date

20260120

Claims (10)

1. 1. A green and environment-friendly method for extracting persimmon polyphenol from persimmons is characterized by comprising the following steps of: S1, taking persimmons as raw materials, cleaning, drying, crushing, adding glacial acetic acid aqueous solution, adjusting pH to 4-5, adding enzyme, mixing, and carrying out enzymolysis to obtain a mixed solution I; S2, extracting the mixed solution I by alcohol, centrifuging, and taking a liquid part to obtain a mixed solution II; S3, adsorbing the mixed solution II by using resin, adding an organic solvent for eluting, collecting an eluent, concentrating and drying to obtain persimmon polyphenol; In the step S1, the enzymolysis comprises a first enzymolysis and a second enzymolysis, the enzymes adopted by the first enzymolysis and the second enzymolysis are different, the enzyme adopted by the first enzymolysis is pectase, and the enzyme adopted by the second enzymolysis consists of hemicellulase and beta-glucosidase.
2. 2. The green and environment-friendly method for extracting persimmon polyphenol from persimmons, which is characterized in that the mass ratio of hemicellulase to beta-glucosidase is 2-4:1.
3. 3. The green and environment-friendly method for extracting persimmon polyphenol from persimmons, which is characterized by comprising the specific steps of taking persimmons as raw materials, washing, drying, crushing, adding pectase, mixing, performing primary enzymolysis, adding hemicellulase and beta-glucosidase, and performing secondary enzymolysis to obtain a mixed solution I.
4. 4. The green and environment-friendly method for extracting persimmon polyphenol from persimmons according to claim 3, wherein the first enzymolysis is carried out at a temperature of 60-70 ℃ for 3 hours, and the second enzymolysis is carried out at a temperature of 35 ℃ for 3 hours.
5. 5. The green environmental protection method for extracting persimmon polyphenol from persimmons according to claim 1, wherein in step S2, the alcohol extraction is alcohol ultrasonic extraction, and the solvent is composed of ethanol and propylene glycol during the alcohol extraction.
6. 6. The green environmental protection method for extracting persimmon polyphenol from persimmons of claim 5, wherein the alcohol extraction comprises a first alcohol extraction and a second alcohol extraction, wherein the ratio of ethanol to propylene glycol in the first alcohol extraction and the ratio of ethanol to propylene glycol in the second alcohol extraction are different.
7. 7. The green environmental protection method for extracting persimmon polyphenol from persimmon of claim 5, wherein said propylene glycol is 70wt% propylene glycol aqueous solution.
8. 8. The green environmental protection method for extracting persimmon polyphenol from persimmon of claim 5, wherein the ethanol is 60wt% of ethanol water solution.
9. 9. The green environmental protection method for extracting persimmon polyphenol from persimmon of claim 1, wherein in step S3, the resin comprises one of Sephadex gel resin and macroporous adsorption resin.
10. 10. The green and environment-friendly method for extracting persimmon polyphenol from persimmons according to claim 1, wherein in the step S3, the organic solvent is an ethanol aqueous solution with the concentration of 40-80 wt%.

Description

Environment-friendly method for extracting persimmon polyphenol from persimmons Technical Field The invention relates to the technical field of persimmon polyphenol extraction, in particular to a green and environment-friendly method for extracting persimmon polyphenol from persimmons. Background The persimmon is a fruit of a persimmon genus plant of the family persimmon, is rich in various nutritional ingredients, wherein persimmon polyphenol is used as an important bioactive substance, has multiple functions of resisting oxidation, resisting inflammation, resisting bacteria, reducing blood fat, protecting cardiovascular and the like, and has wide application prospects in various fields of food additives, health-care products, cosmetics, medicines and the like, so that the efficient extraction of the persimmon polyphenol from the persimmon is of great significance. Along with the continuous improvement of environmental awareness, the greening of the extraction process has become the hot spot and key point of the current research, however, when the persimmon polyphenol is extracted by the green environment-friendly method, the extraction efficiency and the product purity are always challenged, so that the green environment-friendly method for extracting the persimmon polyphenol with high purity and high yield is provided, and has important significance. Disclosure of Invention The invention provides a green and environment-friendly method for extracting persimmon polyphenol from persimmons, which solves the problems of insufficient yield and purity of persimmon polyphenol extraction in the related technology. The technical scheme of the invention is as follows: The invention provides a green and environment-friendly method for extracting persimmon polyphenol from persimmons, which comprises the following steps of: S1, taking persimmons as raw materials, cleaning, drying, crushing, adding glacial acetic acid aqueous solution, adjusting pH to 4-5, adding enzyme, mixing, and carrying out enzymolysis to obtain a mixed solution I; S2, extracting the mixed solution I by alcohol, centrifuging, and taking a liquid part to obtain a mixed solution II; S3, adsorbing the mixed solution II by using resin, adding an organic solvent for eluting, collecting an eluent, concentrating and drying to obtain persimmon polyphenol; In the step S1, the enzymolysis comprises a first enzymolysis and a second enzymolysis, the enzymes adopted by the first enzymolysis and the second enzymolysis are different, the enzyme adopted by the first enzymolysis is pectase, and the enzyme adopted by the second enzymolysis consists of hemicellulase and beta-glucosidase. As a further technical scheme, in the step S1, the mass ratio of the persimmon to the enzyme is 600:0.2-0.4, preferably 200:0.1. As a further technical scheme, the mass ratio of the hemicellulase to the beta-glucosidase is 2-4:1. According to a further technical scheme, the step S1 specifically comprises the steps of taking persimmons as raw materials, cleaning, drying, crushing, adding pectase, mixing, performing primary enzymolysis, adding hemicellulase and beta-glucosidase, and performing secondary enzymolysis to obtain a mixed solution I. As a further technical scheme, the first enzymolysis is carried out at a temperature of 60-70 ℃ for 3 hours, and the second enzymolysis is carried out at a temperature of 35 ℃ for 3 hours. As a further technical scheme, in the step S2, the alcohol extraction is alcohol ultrasonic extraction, and the solvent during the alcohol extraction is composed of ethanol and propylene glycol. As a further technical scheme, in step S2, the alcohol extraction includes a first alcohol extraction and a second alcohol extraction, where the ratio of ethanol to propylene glycol in the first alcohol extraction is different from that in the second alcohol extraction. As a further technical scheme, in the first alcohol extraction, the mass ratio of the ethanol to the propylene glycol is 5-8:3. As a further technical scheme, in the second alcohol extraction, the mass ratio of the ethanol to the propylene glycol is 3:5-8. The invention adopts mixed solvents with different ethanol and propylene glycol ratios for two alcohol extractions, which can further improve the extraction yield and purity of persimmon polyphenol, wherein the first alcohol extraction uses ethanol as a main component to preferentially dissolve persimmon polyphenol with relatively weak polarity; the second alcohol extraction is to extract persimmon polyphenol with stronger polarity by taking propylene glycol as a main component, so that the problem of incomplete extraction by a single solvent is avoided, and the extraction yield and purity are further improved. As a further technical scheme, the ethanol is 60wt% ethanol water solution. As a further technical scheme, the propylene glycol is a 70wt% propylene glycol aqueous solution. As a further technical scheme, in step S3, the resin includes one of