CN-121974979-A - Application of artificially designed and synthesized small peptide AV5P-1 in prevention and treatment of plant RNA virus

Abstract

The invention discloses an application of artificially designed and synthesized small peptide AV5P-1 in preventing and treating plant RNA viruses, belonging to the field of biotechnology. The invention obtains the small peptide AV5P-1 through artificial intelligence design, and the amino acid sequence is shown as SEQ ID NO. 1. The small peptide has small molecular weight and proper hydrophilicity, can efficiently permeate plant tissues, directly targets viral replicase and inhibits the activity of the viral replicase, thereby effectively blocking the replication and diffusion of viruses after virus infection. The small peptide can exert remarkable antiviral effect in various crops such as tobacco and the like through exogenous spraying, and does not influence the normal growth of plants. The invention provides a brand new idea and a reliable technical scheme for developing a novel plant virus prevention and treatment preparation with high efficiency and low residue.

Inventors

• ZHANG TIANYE
• YANG JIAN

Assignees

• 宁波大学

Dates

Publication Date

20260505

Application Date

20260408

Claims (10)

1. 1. A small peptide is characterized in that the amino acid sequence of the small peptide is shown as SEQ ID NO. 1.
2. 2. Use of the small peptide of claim 1 for the preparation of a medicament for combating RNA viral infection in plants.
3. 3. The use according to claim 2, characterized in that the RNA viruses comprise turnip mosaic virus and tomato mosaic virus.
4. 4. A medicament for combating RNA virus infection in plants, characterized in that the active ingredient comprises a small peptide according to claim 1.
5. 5. A method for controlling RNA viral infection in a plant, comprising the step of exogenously spraying the small peptide of claim 1 or the drug of claim 4 into the plant.
6. 6. The method of claim 5, wherein the site of spraying is a leaf blade and the concentration of the small peptide during spraying is 500 μm.
7. 7. The method of claim 5, wherein the plant comprises tobacco.
8. 8. A method for improving the resistance of a plant to RNA viruses, comprising the step of exogenously spraying the plant with the small peptide of claim 1 or the drug of claim 4.
9. 9. The method of claim 8, wherein the site of spraying is a leaf blade and the concentration of the small peptide during spraying is 500 μm.
10. 10. The method of claim 8, wherein the plant comprises tobacco.

Description

Application of artificially designed and synthesized small peptide AV5P-1 in prevention and treatment of plant RNA virus Technical Field The invention relates to the field of biotechnology, in particular to application of small peptide AV5P-1 artificially designed and synthesized in preventing and treating plant RNA viruses. Background Plant virus diseases are important factors for restricting agricultural production, especially potato virus Y is used as a widely transmitted RNA virus, and can infect various important economic crops such as tobacco, wheat and the like, cause symptoms such as leaf yellowing, leaf vein necrosis, growth retardation and the like, and seriously reduce the yield and quality of crops. At present, the prevention and treatment of the viruses mainly depend on chemical agents and traditional antiviral breeding, but the methods have obvious limitations that the chemical agents usually have environmental residue risks, part of the agents can only play a role in preventing the viruses before infection, once the viruses invade plant tissues and are applied, the replication and the diffusion of the viruses are often difficult to effectively inhibit, and the traditional antiviral breeding has long period, high cost and difficulty in coping with the rapid variation of the viruses. Therefore, development of an antiviral agent that is highly effective, environmentally friendly and has a broad spectrum has become an urgent need in the current plant protection field. The small peptide is used as a small molecular substance consisting of 2-100 amino acids, has the characteristics of high activity, easy synthesis, penetrability of plant tissues and the like, and has potential application value in stress resistance and immune regulation of plants. Traditionally, screening antiviral small peptides from plant sources has relied on extensive experimental verification, the process is time-consuming and laborious, and the obtained small peptides often have limited activity or ambiguous action targets, and effective intervention on the viral replication process is difficult to achieve. In recent years, with the development of artificial intelligence technology in the field of drug design, it has become possible to obtain small peptides with specific targeting functions through computational simulation and rational design, which provides a new idea for developing novel anti-plant virus preparations. However, the prior art has not been successful in directly applying artificially designed small peptides to plant RNA virus control, and in particular, in targeting viral replicase and blocking its activity, the challenges of low efficiency of small peptide design, insufficient in vivo verification, and the like are still faced. In addition, the prior antiviral agents have obvious defects in the application mode that most of the agents can be effective after being used before virus infection, and the inhibition effect on infected plants is very small. This limitation arises from the difficulty of the agent to penetrate plant tissue or from the inability to precisely target viral replication in vivo. Therefore, how to design a small peptide molecule that can efficiently enter plant cells, specifically bind to viral replicase and inhibit its function becomes a key difficulty in solving the above-mentioned problems. Although artificial intelligence aided design provides a new approach for small peptide development, multiple technical bottlenecks such as small peptide stability, broad spectrum, host safety and the like are still faced in practical application, and breakthrough through innovative design and experimental verification is needed. Disclosure of Invention The invention aims to provide an application of artificially designed and synthesized small peptide AV5P-1 in preventing and treating plant RNA viruses so as to solve the problems in the prior art. The artificial designed small peptide AV5P-1 provided by the invention has small molecular weight and good permeability, and can inhibit the activity of viral replicase in a targeted manner. The preparation can effectively block the replication and diffusion of viruses after the infection of the viruses, and overcomes the defect that the traditional medicament is limited in prevention. The small peptide is simple and convenient to prepare and environment-friendly, can exert remarkable antiviral effect in crops through exogenous spraying, does not influence normal growth of plants, and provides a brand new scheme for developing novel plant virus control preparations with high efficiency and low residue. In order to achieve the above object, the present invention provides the following solutions: The invention provides a small peptide, the amino acid sequence of which is shown as SEQ ID NO. 1. The invention also provides application of the small peptide in preparing a medicine for resisting plant RNA virus infection. Alternatively, the RNA virus includes turni