CN-121974981-A - Jellyfish polypeptide with tyrosinase inhibitory activity and application thereof

Abstract

The invention discloses jellyfish polypeptide with tyrosinase inhibitory activity and application thereof, and belongs to the technical field of biology. The jellyfish crude polypeptide is prepared, and a jellyfish polypeptide GEPINPEAWLWYYKYVG is identified and selected from the jellyfish crude polypeptide, and experiments prove that the jellyfish polypeptide provided by the invention can inhibit tyrosinase activity in vitro, can inhibit melanin secretion of B16-F10 mouse melanoma cells, and can inhibit tyrosinase activity in cells. Therefore, the jellyfish polypeptide can be used as a tyrosinase inhibitor and an anti-melanin synthesis agent and applied to the fields of cosmetics, medical treatment and the like. The jellyfish polypeptide provided by the invention is proved to have no cytotoxicity through experiments, is derived from the ocean, and has no disease risk and religion barrier of land-based animals.

Inventors

• YU HUAHUA
• Sheng Juhua
• WANG WEIFENG
• LI PENGCHENG

Assignees

• 水母娘娘海洋生物科技有限公司
• 中国科学院海洋研究所

Dates

Publication Date

20260505

Application Date

20260204

Claims (6)

1. 1. A jellyfish polypeptide with tyrosinase inhibitory activity is characterized in that the amino acid sequence of the jellyfish polypeptide is shown as SEQ ID NO. 3.
2. 2. Use of the jellyfish polypeptide of claim 1 in any one of the following: (1) Preparing a tyrosinase inhibitor; (2) Preparing a whitening and skin-protecting cosmetic; (3) Preparing a health product for improving chloasma; (4) Preparing the medicine for treating skin pigmentation diseases.
3. 3. A tyrosinase inhibitor comprising the jellyfish polypeptide of claim 1.
4. 4. A cosmetic for whitening and caring skin, comprising the jellyfish polypeptide of claim 1.
5. 5. A health product for improving chloasma, which is characterized by comprising the jellyfish polypeptide of claim 1.
6. 6. A medicament for treating skin pigmentation disorders comprising the jellyfish polypeptide of claim 1.

Description

Jellyfish polypeptide with tyrosinase inhibitory activity and application thereof Technical Field The invention relates to the technical field of biology, in particular to jellyfish polypeptide with tyrosinase inhibitory activity and application thereof. Background Melanin is a substance produced by normal human cells to balance skin color and resist skin irritation and damage caused by ultraviolet rays, and is the first barrier for protecting human skin and weakening photoaging, and is mainly present in keratinocytes and melanocytes. Normal production of melanin helps to protect the skin from uv damage, but abnormal accumulation of melanin can lead to skin problems such as chloasma, freckles and post-inflammatory pigmentation, and even can induce melanoma. Tyrosinase is the rate-limiting enzyme in the process of melanin formation, and participates in two main processes in catalyzing melanin synthesis, first in catalyzing tyrosine formation of 3, 4-dihydroxyphenylalanine (dopa) in the human body. After the dopa is formed, tyrosinase further shows the activity of bisphenol enzyme, and the dopa is catalyzed to continuously synthesize dopa quinone, then substances such as 5, 6-dihydroxyindole, 5, 6-dihydroxyindole carboxylic acid and the like are synthesized, and finally brown pigment and melanin are formed through oxidative polymerization. Therefore, the purpose of inhibiting melanin synthesis can be achieved by inhibiting tyrosinase activity. Currently, whitening cosmetics and medicines often contain chemical whitening components, such as kojic acid, hydroquinone, nicotinamide and the like, which have certain irritation and cytotoxicity, and can weaken skin barrier, damage sensitive skin and even risk causing cancer. In recent years, the number of jellyfish is increased in an explosive manner under the influence of factors such as global warming, eutrophication and the like, and the main component of jellyfish organic matters is protein, so that the jellyfish is a high-quality raw material of active polypeptide. However, jellyfish polypeptides having a whitening effect have been rarely studied at present. Disclosure of Invention The invention aims to provide jellyfish polypeptides with tyrosinase inhibitory activity and application thereof, which are used for solving the problems of the prior art, and the jellyfish polypeptides can inhibit the tyrosinase activity in vitro and inhibit melanin secretion of B16-F10 mouse melanoma cells and inhibit the tyrosinase activity in cells. In order to achieve the above object, the present invention provides the following solutions: The invention provides jellyfish polypeptide with tyrosinase inhibitory activity, and the amino acid sequence of the jellyfish polypeptide is shown as SEQ ID NO. 3. The invention also provides application of the jellyfish polypeptide in any one of the following: (1) Preparing a tyrosinase inhibitor; (2) Preparing a whitening and skin-protecting cosmetic; (3) Preparing a health product for improving chloasma; (4) Preparing the medicine for treating skin pigmentation diseases. The invention also provides a tyrosinase inhibitor, which comprises the jellyfish polypeptide. The invention also provides a cosmetic for whitening and protecting skin, which comprises the jellyfish polypeptide. The invention also provides a health product for improving chloasma, which comprises the jellyfish polypeptide. The invention also provides a medicine for treating skin pigmentation diseases, which comprises the jellyfish polypeptide. The invention discloses the following technical effects: The jellyfish crude polypeptide is prepared, and a jellyfish polypeptide GEPINPEAWLWYYKYVG is identified and selected from the jellyfish crude polypeptide, and experiments prove that the jellyfish polypeptide provided by the invention can inhibit tyrosinase activity in vitro, and can inhibit melanin secretion of B16-F10 mouse melanoma cells and inhibit intracellular tyrosinase activity. Therefore, the jellyfish polypeptide can be used as a tyrosinase inhibitor and an anti-melanin synthesis agent and applied to the fields of cosmetics, medical treatment and the like. The jellyfish polypeptide provided by the invention is proved to have no cytotoxicity through experiments, is derived from the ocean, and has no disease risk and religion barrier of land-based animals. Drawings In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings that are needed in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art. FIG. 1 shows the inhibition of tyrosinase activity by jellyfish active peptides at different concentrations; FIG. 2 shows the survival rate of B16-F10 mouse melanoma cells aft