CN-121974987-A - Antibacterial peptide with antibacterial activity of multiple plant pathogenic bacteria, biological antibacterial agent and application thereof

Abstract

The invention discloses an antibacterial peptide with multiple plant pathogenic bacteria antibacterial activity, a biological antibacterial agent and application thereof, wherein the amino acid sequence of the antibacterial peptide is FKLIRNLIILRQAWKLIKRRR and is labeled as RR-21. Experiments show that the antibacterial peptide has a similar alpha-helical structure with the known antibacterial peptide LL-37, has antibacterial activity on plant pathogenic bacteria represented by pseudomonas syringae and plant pathogenic fungi represented by fusarium pseudograminearum and fusarium oxysporum, has more remarkable antibacterial effect at the MIC of RR-21 of 4-8 ug/mL compared with the LL-37 polypeptide, and simultaneously has no hemolytic activity at the conventional antibacterial concentration (less than or equal to 16 mug/mL), fully proves that the antibacterial peptide has high biological safety on eukaryotic cells while playing multiple antibacterial effects, and can be used as an active ingredient of a biological antibacterial agent, and the biological antibacterial agent has the potential of developing an efficient and safe anti-plant disease medicament or a plant green pesticide.

Inventors

• LIU YONGQIN
• YU TAO

Assignees

• 兰州大学

Dates

Publication Date

20260505

Application Date

20260210

Claims (7)

1. 1. An antibacterial peptide with multiple plant pathogenic bacteria antibacterial activity is characterized in that the amino acid sequence of the antibacterial peptide is shown as SEQ ID NO.1 and is recorded as RR-21.
2. 2. A biological antibacterial agent for plant pathogenic bacteria, characterized in that the biological antibacterial agent takes the antibacterial peptide as defined in claim 1 as a core active ingredient, and further comprises an agriculturally acceptable carrier, auxiliary agent, diluent or excipient.
3. 3. A plant pathogenic bacteria biological antibacterial agent according to claim 2, wherein the biological antibacterial agent is in the form of any one of aqueous solution, powder, wettable powder, suspension, emulsifiable concentrate or microcapsule.
4. 4. A plant pathogenic organism antibacterial agent according to claim 2 wherein the plant pathogenic bacteria include plant pathogenic bacteria and plant pathogenic fungi.
5. 5. A plant pathogenic organism antibacterial agent according to claim 4, wherein the plant pathogenic bacterium is pseudomonas syringae.
6. 6. A plant pathogenic organism antibacterial agent according to claim 4, wherein the plant pathogenic fungus is fusarium pseudograminearum and/or fusarium oxysporum.
7. 7. Use of a biological antibacterial agent according to any one of claims 2 to 6 for the preparation of a plant disease resistant medicament or biopesticide.

Description

Antibacterial peptide with antibacterial activity of multiple plant pathogenic bacteria, biological antibacterial agent and application thereof Technical Field The invention relates to the technical field of biological pharmacy, in particular to an antibacterial peptide with multiple plant pathogenic bacteria antibacterial activity, a biological antibacterial agent and application thereof. Background Phytopathogenic bacteria and fungi are one of the most damaging biohazard factors in global agricultural production, severely restricting the yield stability and quality safety of crops for a long time. Plant pathogenic microorganisms represented by Pseudomonas syringae (Pseudomonas syringae), fusarium pseudograminearum (Fusariumpseudograminearum) and Fusarium oxysporum (Fusariumoxysporum) can respectively trigger various plant diseases which are high in incidence and difficult to control, such as bacterial alternaria disease, root rot, stem basal rot, vascular bundle wilt and the like. The diseases widely occur in wheat, corn, rice and various important economic crops, and have the characteristics of high transmission speed, long latency period, high recurrence rate and the like, so that large-area yield reduction and even harvest failure are often caused, and great economic losses are brought to agricultural production and agricultural product supply. At present, the prevention and control of the plant diseases in agricultural production still mainly depend on chemical pesticides. However, long-term or unreasonable use of chemical pesticides not only easily causes environmental pollution and exceeds standard pesticide residues of agricultural products, but also accelerates the generation and diffusion of pathogen resistance, so that the control effect is gradually reduced, and a vicious circle which is more difficult to control is formed. The problems become key bottlenecks for restricting the green development and sustainable production of modern agriculture, and form an increasingly serious threat to national food safety and ecological safety. The antibacterial peptide is used as an important component of the natural immune system of organisms, is generally composed of 20-100 amino acids, and has the characteristics of positive charge, amphipathy, broad-spectrum antibacterial activity and the like. Compared with the traditional chemical pesticides, the antibacterial peptide has the advantages of unique action mechanism, difficult drug resistance induction, good biodegradability and the like, and is considered as an important candidate molecule of a new generation of green biopesticide in recent years. Various antibacterial peptides derived from animals, plants or microorganisms have been reported in the prior art, but most antibacterial peptides have the problems of narrow antibacterial spectrum, limited activity on plant pathogenic fungi and the like. In addition, aiming at important plant pathogenic fungi such as fusarium pseudograminearum and fusarium oxysporum, the existing antibacterial peptide products are still limited, and antibacterial peptide molecules which have high-efficiency inhibition effects on plant pathogenic bacteria and fungi and are suitable for agricultural application are not available. Disclosure of Invention Based on the above, the invention aims to provide an antibacterial peptide with simple structure, easy synthesis, safety and environmental protection and multiple plant pathogenic bacteria antibacterial activity and a biological antibacterial agent using the antibacterial peptide as a core active ingredient. The invention also aims to provide the application of the biological antibacterial agent in preparing plant disease resistant medicines or biological pesticides so as to meet the actual requirements of green prevention and control of plant diseases. In order to achieve the purpose, the invention adopts the following technical scheme: the invention provides an antibacterial peptide with multiple plant pathogenic bacteria antibacterial activity, the amino acid sequence of which is FKLIRNLIILRQAWKLIKRRR (SEQ ID NO. 1), which is denoted as RR-21. The invention also provides a plant pathogenic bacteria biological antibacterial agent which takes the antibacterial peptide as a core active ingredient and also comprises an agriculturally acceptable carrier, an auxiliary agent, a diluent or an excipient. As a further preferable mode of the technical scheme, the biological antibacterial agent is any one of water agent, powder, wettable powder, suspending agent, emulsifiable concentrate or microcapsule. Further, the plant pathogenic bacteria include plant pathogenic bacteria and plant pathogenic fungi. Further, the plant pathogenic bacteria are pseudomonas syringae, and the plant pathogenic fungi are fusarium pseudograminearum and/or fusarium oxysporum. The biological antibacterial agent can be used for preparing plant disease resistant medicines or biological pesticides. By adopting th