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CN-121974990-A - ZmGATA11 protein and application of coding gene thereof in drought resistance of plants

CN121974990ACN 121974990 ACN121974990 ACN 121974990ACN-121974990-A

Abstract

The invention discloses ZmGATA protein and application of a coding gene thereof in drought resistance of plants. The invention belongs to the technical field of biology, and particularly relates to ZmGATA protein and application of a coding gene thereof in drought resistance of plants. The protein disclosed by the invention is a protein with an amino acid sequence shown as SEQ ID No.2, wherein A1) is subjected to substitution and/or deletion and/or addition of amino acid residues to obtain a protein which has more than 80% of identity with the protein shown as A1) and has the same function, A3) is connected with a protein tag at the N end or/and the C end of A1) or A2), and the protein ZmGATA is overexpressed, so that the drought resistance of plants can be improved, and the yield of plants can be improved.

Inventors

  • WANG YU
  • GONG ZHIZHONG
  • WANG YALIN

Assignees

  • 中国农业大学

Dates

Publication Date
20260505
Application Date
20240226

Claims (10)

  1. 1. A protein which is any one of the following: A1 Protein with the amino acid sequence shown as SEQ ID No. 2; A2 Protein with more than 80% of identity and same function as the protein shown in A1) obtained by substituting and/or deleting and/or adding amino acid residues of the protein of A1); A3 Fusion proteins obtained by ligating protein tags at the N-terminal or/and C-terminal of A1) or A2).
  2. 2. The protein of claim 1, wherein the protein is derived from corn.
  3. 3. A biomaterial associated with the protein of claim 1 or 2, said biomaterial being any one of the following: B1 A nucleic acid molecule encoding the protein of claim 1; b2 An expression cassette comprising the nucleic acid molecule of B1); B3 A recombinant vector comprising the nucleic acid molecule of B1) or a recombinant vector comprising the expression cassette of B2); B4 A recombinant microorganism comprising the nucleic acid molecule of B1), or a recombinant microorganism comprising the expression cassette of B2), or a recombinant microorganism comprising the recombinant vector of B3); b5 A transgenic plant cell line comprising the nucleic acid molecule of B1) or a transgenic plant cell line comprising the expression cassette of B2); b6 A transgenic plant tissue comprising the nucleic acid molecule of B1) or a transgenic plant tissue comprising the expression cassette of B2); B7 A transgenic plant organ comprising the nucleic acid molecule of B1) or a transgenic plant organ comprising the expression cassette of B2).
  4. 4. The biological material according to claim 3, wherein the nucleic acid molecule of B1) is a gene represented by E1) or E2) as follows: E1 A cDNA molecule or a DNA molecule having a coding sequence of SEQ ID No. 3; E2 Nucleotide is a cDNA molecule or a DNA molecule of SEQ ID No. 1.
  5. 5. The application is characterized by being any one of the following: U1) use of the protein or the substance regulating gene expression or the substance regulating the activity or the content of the protein according to claim 1 or 2 for regulating drought stress resistance of plants; u2) use of the protein or the substance regulating gene expression or the substance regulating the activity or the content of the protein according to claim 1 or 2 for the preparation of a product regulating drought stress resistance of plants; u3) use of the protein or gene-regulating expression substance or protein activity or content-regulating substance according to claim 1 or 2 for cultivating drought-resistant plants; U4) use of the protein or the substance regulating the expression of a gene or the substance regulating the activity or the content of said protein according to claim 1 or 2 for the preparation of a product for cultivating drought-resistant plants; U5) use of a protein or a substance regulating the expression of a gene or a substance regulating the activity or content of said protein according to claim 1 or 2 in plant breeding.
  6. 6. The method according to claim 5, wherein the substance regulating the expression of the gene or the substance regulating the activity or the content of the protein is a biological material related to the protein, the biological material being any one of the following B1) to B7): B1 A nucleic acid molecule encoding the protein of claim 1; b2 An expression cassette comprising the nucleic acid molecule of B1); B3 A recombinant vector comprising the nucleic acid molecule of B1) or a recombinant vector comprising the expression cassette of B2); B4 A recombinant microorganism comprising the nucleic acid molecule of B1), or a recombinant microorganism comprising the expression cassette of B2), or a recombinant microorganism comprising the recombinant vector of B3); b5 A transgenic plant cell line comprising the nucleic acid molecule of B1) or a transgenic plant cell line comprising the expression cassette of B2); b6 A transgenic plant tissue comprising the nucleic acid molecule of B1) or a transgenic plant tissue comprising the expression cassette of B2); B7 A transgenic plant organ comprising the nucleic acid molecule of B1) or a transgenic plant organ comprising the expression cassette of B2).
  7. 7. A method for controlling drought stress resistance of a plant, which comprises controlling the activity and/or content of the protein of claim 1 or 2 in a target plant, or/and the expression level of the gene encoding the protein of claim 1 or 2.
  8. 8. The method according to claim 7, wherein the regulation of the activity and/or the content of the protein according to claim 1 or 2 in the target plant, or/and the expression level of the gene encoding the protein according to claim 1 or 2, comprises introducing the gene encoding the protein into a recipient plant to obtain a target plant having a plant drought stress resistance higher than that of the recipient plant, wherein the gene encoding the protein according to claim 1 or 2.
  9. 9. A method for growing a plant with altered drought stress resistance, comprising 1) increasing, enhancing and/or upregulating the expression level of a gene encoding the protein according to claim 1 in a plant of interest, or/and increasing, enhancing and/or upregulating the activity and/or content of a gene encoding the protein according to claim 1, to obtain a plant with increased drought stress resistance; 2) Inhibiting or reducing or silencing the expression level of a gene encoding the protein of claim 1 in a plant of interest, or/and inhibiting or reducing or silencing the activity and/or content of a gene encoding the protein of claim 1, to obtain a plant with reduced drought stress resistance.
  10. 10. The method according to any one of claims 7 to 9, wherein the plant is any one of the following: n1) monocotyledonous plants: n2) gramineae plants; n3) a gramineous plant; N4) zea plants; N5) corn.

Description

ZmGATA11 protein and application of coding gene thereof in drought resistance of plants Technical Field The invention belongs to the technical field of biology, and particularly relates to ZmGATA protein and application of a coding gene thereof in drought resistance of plants. Background The climate factors seriously influence the growth of crops, find out related genes involved in plant drought signal transduction and influencing drought resistance, research the gene functions of the genes, and provide theoretical basis for improving the drought resistance of crops and reducing the yield reduction caused by drought through genetic improvement. Editing or over-expressing a certain or some specific genes by genetic engineering means to improve stress resistance of plants is one of the modes of molecular breeding. The technology breaks through the limit of interspecific hybridization, is one of effective ways for improving stress resistance of crops, can improve the yield of crops under adverse circumstances, and has important significance for solving the food shortage caused by environmental stress. Drought can cause osmotic stress in plants, osmotic pressure in plants is lower than that of the environment (such as soil solution), and plants cannot absorb water or even lose water. There are two pathways for osmotic stress, ABA-dependent and ABA-independent. When subjected to environmental stress, depending on the ABA pathway, ABA, PYL, PYR, RCAR is an ABA receptor capable of binding to ABA. The activity of PP2C protein phosphatase is inhibited, so that SnRK2 has kinase activity, can phosphorylate downstream transcription factors and the like, thereby regulating and controlling the expression of downstream stress response genes, inhibiting the expansion of air holes, regulating and controlling the sensitivity of ABA and producing other responses. Independent of ABA pathway, DREB2A is phosphorylated, regulating downstream stress response gene expression. Drought stress can induce abscisic acid (ABA) to be produced in plants, can timely guide stomata to close so as to reduce water loss in the plants, and can activate expression of a large number of drought-related genes so as to cause various drought stress responses of the plants. ABA acts as an extremely important plant hormone, and plays a key role in stomatal closure, expression of stress response genes, and the like. Plants, due to their sessile nature, need to function through intracellular, elaborate signal transduction systems when subjected to stress, with the important phytohormone abscisic acid (ABA) playing a vital role. Under drought stress, a series of reactions are initiated in plants, including changes in stomatal movement, regulation of growth and development, etc., to make the plants effective in a diverse environment. In complex signal regulation networks, transcription factors play a very important role, and plants regulate the expression of drought response genes through a series of key transcription factors, so that plants survive in stress. The important transcription factors are key components involved in an ABA signal pathway, including ABI3, ABI4, ABI5, ABFs and the like, and regulate the dormancy of seeds, the growth and development of plants and the like. The transcription factor of GATA family is a kind of gene specific to seed plant, and is involved in embryo development of plant, differentiation of lateral organ, adversity response and other aspects. Corn is a crop which is easily affected by drought stress, so that the corn variety is improved by a genetic engineering means, the drought resistance is improved, and the method has important significance for yield reduction caused by drought. With the completion of sequencing of the genome of the maize inbred lines such as B73, mo17 and the like, the genetic background of the maize is clearer. Meanwhile, the inbred line easy to genetic transformation is continuously sequenced and developed, so that the efficiency of the transgenic over-expression and gene editing technology is greatly improved, such as LH244 inbred line. The transformation efficiency of the inbred line is higher than that of most of the inbred lines known at present, transgenic over-expression or gene editing plants are easy to obtain through transformation, technical support is provided for genetic trait improvement by utilizing molecular breeding, and the inbred line has application value for reducing corn yield reduction caused by abiotic stress such as drought and the like. Disclosure of Invention The technical problem to be solved by the invention is how to regulate and control drought stress resistance of plants. In order to solve the problems existing in the prior art, the invention provides a protein. The protein provided by the invention can be any one of the following proteins: A1 Protein with the amino acid sequence shown as SEQ ID No. 2; A2 Protein with more than 80% of identity with the protein shown in