CN-121974992-A - Application of ZmERF83 in improving stress resistance of crops

Abstract

The invention belongs to the technical field of plant genetic engineering, and particularly relates to application of ZmERF83 in improving stress resistance of crops. According to the invention, zmERF83 is introduced into Arabidopsis thaliana to screen positive clones for drought and salt stress measurement, and the result shows that ZmERF83 can effectively improve the stress resistance of crops and improve the physiological state of crops under drought or salt stress conditions. Therefore, the invention provides application of the maize-derived AP2/ERF transcription factor gene ZmERF83 and the encoded protein thereof to enhancing the tolerance of plants to drought, high salt and other abiotic stresses.

Inventors

• CHI CHENG
• WANG MENGQI
• ZHU JIANTANG

Assignees

• 济南大学

Dates

Publication Date

20260505

Application Date

20260407

Claims (8)

1. The application of ZmERF83 in improving crop stress resistance is characterized in that NCBI protein number of ZmERF83 is XP_003548854, and stress resistance is the capability of crops in saline-alkali environment.
2. 2. The use of ZmERF83 in improving stress resistance of crops according to claim 1, wherein the mRNA sequence of ZmERF83 is accession number xm_003548806 in GenBank.
3. 3. Use of ZmERF83 according to claim 1 for improving stress resistance of crops, wherein said use is: (1) The survival rate of the plants in a high-salt environment is improved by improving the expression of ZmERF83 in crops; (2) ZmERF83 or an agent that promotes ERF83 expression in corn is used to prepare a crop growth promoter.
4. 4. The use of ZmERF83 according to claim 3 for improving stress resistance of crops, wherein the method for improving expression of ZmERF83 in crops in (1) comprises genetic engineering method and chemical reagent method.
5. 5. The application of ZmERF83 in improving stress resistance of crops as claimed in claim 4, wherein the genetic engineering method comprises constructing an optimized expression vector containing ERF83 gene, transferring into corn by using agrobacterium or gene gun mediation, screening and identifying to realize high expression; Alternatively, the chemical agent method comprises applying a chemical agent to the crop to be treated, thereby enhancing the transcription, translation efficiency, or increasing the gene copy number of the ERF83 gene.
6. 6. The use of ZmERF83 in improving stress resistance of crops according to claim 3, wherein in (1) said high salt environment comprises a high salt environment caused by exceeding of salt content in soil, planting in coastal areas, excessive fertilization, and sewage irrigation.
7. 7. The use of ZmERF83 in improving stress tolerance in crops according to claim 3, wherein in (2) the agent that promotes expression of ERF83 in maize comprises a compound or composition that stimulates overexpression of ERF83 in maize, and further comprises a related agent that modifies expression of ERF83 in maize plants by genetic engineering means.
8. 8. A method for improving the yield of corn in saline-alkali soil, which is characterized by comprising the step of obtaining a plant with high expression of ZmERF83 by genetic engineering means.

Description

Application of ZmERF83 in improving stress resistance of crops Technical Field The invention belongs to the technical field of plant genetic engineering, and particularly relates to application of ZmERF83 in improving stress resistance of crops. Background The disclosure of this background section is only intended to increase the understanding of the general background of the invention and is not necessarily to be construed as an admission or any form of suggestion that this information forms the prior art already known to those of ordinary skill in the art. AP2/ERF (APETALA 2/ethylene response factor) transcription factor is one of the largest families of transcription factors in plants, playing a key role in plant growth, signal transduction, and response to biotic and abiotic stress. The study of the AP2/ERF transcription factor family of corn (Zea mays l.) as a globally important food and feed crop has great significance for improving crop yield and stress resistance. The AP2/ERF superfamily of maize has been identified and categorized at the genomic level. For example, in the latest B73 RefGen_v5 maize reference genome, 229 AP2/ERF genes were identified in total. These genes can be divided into five branches, 27 AP2 genes, 5 RAV genes, and 197 ERF genes (including DREB subfamilies and ERF subfamilies). Studies have shown that maize AP2/ERF transcription factors play an important role in a variety of biological processes, particularly in abiotic stress responses. For example, zmEREBP, a member of the AP2/ERF family, over-expression of this gene can enhance drought tolerance in maize. The response to abiotic stress of another AP2/ERF transcription factor ZmEREB211 has also been studied. In addition, it has been found that maize transcription factor ZmEREB97 plays a regulatory role in nitrate uptake, whose expression responds rapidly to nitrate treatment, mainly in the main and lateral root primordia. ZmEREB130,130 was found to negatively regulate corn growth and seed size. These studies reveal specific functions of specific AP2/ERF genes in maize growth and development and stress response. In view of the current state of research, the invention considers that although the research of the AP2/ERF family has achieved great achievements, the research of genes of the family still has obvious blank and defects, such as huge ratio of unknown genes, unclear molecular mechanism and lack of deep analysis of key genes, for important crops such as corn. Disclosure of Invention AP2/ERF (APETALA 2/ethylene response factor) is an important family of transcription factors in plants and plays a key role in regulating plant stress response. According to the invention, zmERF83 gene is screened to be transferred into mode crops for drought and salt stress treatment, and research results show that the over-expression of ZmERF83 can obviously improve the stress tolerance of plants. Based on the findings, the present invention provides the following technical solutions: In a first aspect, there is provided the use of ZmERF83 for improving stress resistance in crops. Proved by verification, the crops with high expression of ZmERF83 have better tolerance under drought and salt stress conditions, and the specific performances include: (1) The survival rate of crops (80-90%) is much higher than that of wild type (< 30%); (2) Crops have healthier physiological states, including leaf color and growth status. In the aspect of physiological indexes, crops over-expressing ZmERF83 have lower REL and MDA contents and higher proline and chlorophyll contents, which indicates that the maize plants over-expressing ERF83 have lower membrane damage degree and stronger osmotic regulation capability and can maintain better photosynthetic capability. The mRNA sequence of ZmERF83 is registered as XM_003548806 in GenBank, and the NCBI protein is registered as XP_003548854. In a specific embodiment of the invention, the ZmERF83 gene is derived from leaf blades of maize inbred line B73 trefoil stage seedlings and is obtained by extracting total RNA for reverse transcription. The application of the first aspect at least includes the following aspects: (1) The survival rate of the plants in drought environment is improved by improving the expression of ZmERF83 in crops; (2) The survival rate of the plants in a high-salt environment is improved by improving the expression of ZmERF83 in crops; (3) ZmERF83 or an agent that promotes ERF83 expression in corn is used to prepare a crop growth promoter. The method for improving the expression of ZmERF83 in crops in the step (1) or (2) comprises a genetic engineering method, such as construction of an optimized expression vector containing ERF83 genes, transferring into corn by using agrobacterium or a gene gun to mediate and screening and identifying, realizing high expression, and a chemical reagent method, wherein the transcription and translation efficiency of ERF83 genes is improved or the copy number of