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CN-121975013-A - Anti-human CD83 antibody and application thereof

CN121975013ACN 121975013 ACN121975013 ACN 121975013ACN-121975013-A

Abstract

The invention provides an anti-human CD83 antibody and application thereof, wherein the antibody comprises a heavy chain variable region and a light chain variable region, the amino acid sequences of CDR1, CDR2 and CDR3 of the heavy chain variable region are respectively shown as SEQ ID NO.1, SEQ ID NO.2 and SEQ ID NO.3, and the amino acid sequences of CDR1, CDR2 and CDR3 of the light chain variable region are respectively shown as SEQ ID NO.4, SEQ ID NO.5 and SEQ ID NO. 6. The anti-human CD83 antibody provides a light chain variable region gene and a heavy chain variable region gene of an anti-human CD83 monoclonal antibody, can generate a hybridoma cell strain of a CD83 specific antibody, and can be used for detecting human bacterial infection.

Inventors

  • GUO RUI
  • CUI DONGXU
  • ZHANG HONGXIA
  • LI YUAN
  • LI YUAN
  • LI YIBO
  • QU HAO
  • CHEN JIAAO
  • LU JING

Assignees

  • 天津旷博同生生物技术有限公司

Dates

Publication Date
20260505
Application Date
20260409

Claims (8)

  1. 1. An anti-human CD83 antibody is characterized in that the antibody comprises a heavy chain variable region and a light chain variable region; the amino acid sequences of CDR1, CDR2 and CDR3 of the heavy chain variable region are shown as SEQ ID NO.1, SEQ ID NO.2 and SEQ ID NO.3 respectively; the amino acid sequences of CDR1, CDR2 and CDR3 of the light chain variable region are shown as SEQ ID NO.4, SEQ ID NO.5 and SEQ ID NO.6 respectively.
  2. 2. The anti-human CD83 antibody of claim 1 wherein the amino acid sequences of FR1, FR2, FR3 and FR4 of the heavy chain variable region are shown in SEQ ID NO.7, SEQ ID NO.8, SEQ ID NO.9 and SEQ ID NO.10, respectively; the amino acid sequences of the FR1, FR2, FR3 and FR4 of the light chain variable region are respectively shown as SEQ ID NO.11, SEQ ID NO.12, SEQ ID NO.13 and SEQ ID NO. 14.
  3. 3. The anti-human CD83 antibody of claim 1 wherein the amino acid sequence of the heavy chain variable region of the antibody is shown in SEQ ID NO.15 and the amino acid sequence of the light chain variable region is shown in SEQ ID NO. 16.
  4. 4. A nucleic acid molecule comprising a nucleotide sequence encoding the anti-human CD83 antibody of any one of claims 1-3.
  5. 5. An expression vector comprising the nucleic acid molecule of claim 4.
  6. 6. A cell comprising the nucleic acid molecule of claim 4 or the expression vector of claim 5.
  7. 7. Use of an anti-human CD83 antibody according to any one of claims 1-3 for the preparation of a detection reagent for CD83 molecules.
  8. 8. An immunoassay kit comprising the anti-human CD83 antibody of any one of claims 1-3.

Description

Anti-human CD83 antibody and application thereof Technical Field The invention relates to an antibody for diagnosis and detection, in particular to an anti-human CD83 antibody and application thereof. Background The anti-human CD83 monoclonal antibody is a 43 kD single-chain type I transmembrane glycoprotein and is capable of specifically recognizing and binding to human CD83 molecules. Mainly expressed on the surface of mature Dendritic Cells (DCs), an important marker of mature DCs, often expressed in membrane-bound or soluble form. Membrane CD83 can be detected on a variety of activated immune cells, while membrane CD83 can regulate maturation, activation and homeostasis. Soluble CD83 is elevated in serum from patients with autoimmune diseases and some hematological malignancies and has immunosuppressive function. The antibody has wide application in immunological research, and as CD83 is the specific marker of mature DC, the anti-human CD83 monoclonal antibody can be used for identifying and sorting DC by methods such as flow cytometry, immunohistochemistry and the like, and researching the development, differentiation, function and function of DC in immune response. In addition, certain diseases, such as tumors, autoimmune diseases, infectious diseases, etc., change the number and function of DCs in a patient. Anti-human CD83 monoclonal antibodies can be used to detect the number and phenotype of DCs in a patient's peripheral blood or tissue, aid in disease diagnosis, assess disease progression, and therapeutic efficacy. Flow cytometry is a powerful tool for detecting the expression condition of cell surface protein molecules at the single cell level, and by means of a fluorescent labeled anti-CD 83 monoclonal antibody, the expression condition and the change of the fluorescence intensity of the related cell surface CD83 in a human body can be reflected, so that the method can be used for auxiliary diagnosis, disease monitoring, curative effect evaluation and prognosis judgment of tumors, autoimmune diseases, infectious diseases, transplant rejection and inflammatory diseases. Disclosure of Invention In view of the above, the present invention aims to overcome the defects in the prior art and proposes an anti-human CD83 antibody and use thereof. In order to achieve the above purpose, the technical scheme of the invention is realized as follows: The invention also provides an anti-human CD83 antibody comprising a heavy chain variable region and a light chain variable region; the amino acid sequences of CDR1, CDR2 and CDR3 of the heavy chain variable region are shown as SEQ ID NO.1, SEQ ID NO.2 and SEQ ID NO.3 respectively; the amino acid sequences of CDR1, CDR2 and CDR3 of the light chain variable region are shown as SEQ ID NO.4, SEQ ID NO.5 and SEQ ID NO.6 respectively. Further, the amino acid sequences of FR1, FR2, FR3 and FR4 of the heavy chain variable region are respectively shown as SEQ ID NO.7, SEQ ID NO.8, SEQ ID NO.9 and SEQ ID NO. 10; the amino acid sequences of the FR1, FR2, FR3 and FR4 of the light chain variable region are respectively shown as SEQ ID NO.11, SEQ ID NO.12, SEQ ID NO.13 and SEQ ID NO. 14. Further, the amino acid sequence of the heavy chain variable region of the antibody is shown as SEQ ID NO.15, and the amino acid sequence of the light chain variable region is shown as SEQ ID NO. 16. The invention also provides a nucleic acid molecule comprising a nucleotide sequence encoding said anti-human CD83 antibody. The invention also provides an expression vector comprising the nucleic acid molecule. The invention also provides a cell comprising said nucleic acid molecule or said expression vector. The invention also provides application of the anti-human CD83 antibody in preparing a detection reagent of CD83 molecules. The invention also provides an immunoassay kit which comprises the anti-human CD83 antibody. Compared with the prior art, the invention has the following advantages: The anti-human CD83 antibody provides a light chain variable region gene and a heavy chain variable region gene of an anti-human CD83 monoclonal antibody, can generate a hybridoma cell strain of a CD83 specific antibody, and can be used for detecting human bacterial infection. Drawings FIG. 1 is a diagram showing the purification electrophoresis of an anti-human CD83 antibody according to the embodiment of the present invention; FIG. 2 is a graph showing the affinity detection of anti-human CD83 antibodies according to the examples of the present invention; FIG. 3 is a graph showing the activity of an anti-human CD83-PE antibody according to an embodiment of the present invention. Detailed Description Unless defined otherwise, technical terms used in the following examples have the same meaning as commonly understood by one of ordinary skill in the art to which the present invention pertains. The test reagents used in the following examples are all conventional biochemical reagents unless otherwise specif