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CN-121975027-A - American ginseng polysaccharide fermentation product with myocardial ischemia resisting effect, and preparation method and application thereof

CN121975027ACN 121975027 ACN121975027 ACN 121975027ACN-121975027-A

Abstract

The invention discloses an American ginseng polysaccharide ferment with the function of resisting myocardial ischemia and a preparation method and application thereof, comprising the steps of 1) extracting American ginseng with water, filtering, concentrating to obtain concentrated solution, 2) mixing the concentrated solution with a culture medium containing lactobacillus plantarum, adding sucrose, fermenting to obtain crude fermentation broth, centrifuging, inactivating supernatant, 3) concentrating the inactivated supernatant under reduced pressure to obtain second concentrated solution, 4) adding ethanol into the second concentrated solution, stirring, standing, discarding supernatant, dissolving sediment with water, adding Sevage reagent, extracting, taking upper water phase, dialyzing with water, freeze-drying to obtain crude polysaccharide, 5) dissolving the crude polysaccharide with distilled water, adding into a DEAE column, eluting with deionized water, concentrating under reduced pressure to obtain third concentrated solution, dialyzing the third concentrated solution with a 1000Da dialysis bag, and freeze-drying.

Inventors

  • GAO WENYUAN
  • LI XIA
  • LIU ZHENG
  • ZHAO WEIYI

Assignees

  • 天津大学

Dates

Publication Date
20260505
Application Date
20250311

Claims (5)

  1. 1. A preparation method of American ginseng polysaccharide ferment with the function of resisting myocardial ischemia is characterized by comprising the following steps: 1) Heating and reflux-extracting crushed radix Panacis Quinquefolii with purified water for three times each for 1-2 hr, filtering, concentrating the filtrate under reduced pressure at 55-70deg.C to density of 0.9-1.05g/mL at 55-70deg.C to obtain concentrated solution, and adding purified water for three times which is 7-9, 5-7, and 5-7 times of radix Panacis Quinquefolii in sequence; 2) Mixing the concentrated solution with MRS culture medium containing lactobacillus plantarum according to the volume ratio of 96-98:4-2, adding sucrose with the final concentration of 2%, fermenting at 37 ℃ for 36-48 hours to obtain crude fermentation broth, centrifuging, and inactivating the supernatant to obtain an inactivated supernatant; the lactobacillus plantarum is Lactobacillus plantarum ATCC8014; 3) Concentrating the inactivated supernatant to a density of 1.05-1.15g/mL at 55-70 ℃ under reduced pressure to obtain a second concentrated solution; 4) Adding 4-5 times of absolute ethyl alcohol or 80-90% ethanol water solution with volume concentration into the second concentrated solution, stirring uniformly, standing for 24-36 hours, discarding supernatant, dissolving precipitate with distilled water, adding Sevage reagent, extracting until clear layering, no middle layer flocculent and white substance, taking upper water phase, dialyzing with deionized water for 24-36 hours with 1000Da dialysis bag, and lyophilizing to obtain crude polysaccharide; 5) Dissolving the crude polysaccharide with distilled water, adding into DEAE column, eluting with deionized water for 2 times of column volume, collecting 3-8 times of sugar fraction, concentrating under reduced pressure to 1/2 or 1/3 of sugar fraction volume to obtain third concentrated solution, dialyzing with deionized water for 24-36 hr with 1000Da dialysis bag, and lyophilizing to obtain radix Panacis Quinquefolii polysaccharide fermented product with myocardial ischemia resisting effect.
  2. 2. The method according to claim 1, wherein the rotational speed of the centrifugation is 2000-3500rpm and the time of the centrifugation is 10-15min.
  3. 3. The method according to claim 1, wherein the inactivating step comprises heating to 62-65deg.C for 20-35min, heating to 75-80deg.C, and holding for 15s-16s.
  4. 4. A American ginseng polysaccharide ferment having an anti-myocardial ischemia effect prepared by the preparation method of one of claims 1 to 3.
  5. 5. The use of the American ginseng polysaccharide fermentation product with the function of resisting myocardial ischemia as claimed in claim 4 for preparing medicaments for resisting myocardial ischemia.

Description

American ginseng polysaccharide fermentation product with myocardial ischemia resisting effect, and preparation method and application thereof Technical Field The invention belongs to the field of American ginseng extraction and application, and particularly relates to an American ginseng polysaccharide fermentation product with an anti-myocardial ischemia effect, a preparation method and application thereof. Background American ginseng (Panax quinquefolius L.) is a perennial herb of Panax of Araliaceae. American ginseng was first found in the Atlantic coastal jungle in Montreal, and was named for the place of production, and was of the same species as ginseng. The medicinal and edible parts are dry roots and rhizomes, which are firstly carried in the 'additional herbal preparation', bitter and cold is cleared, sweet and cold is supplemented, heart, lung and kidney channels are cold-supplementing products, and the medicinal and edible parts are good for supplementing qi and nourishing yin, clearing fire and promoting the production of body fluid, and are mainly used for treating deficiency of both qi and yin. American ginseng has been formally incorporated into the catalogue of traditional Chinese medicinal materials as well as food in 2023 and Wei Jian attuned to line 9. Modern medicine proves that the chemical components of American ginseng mainly comprise saponins, polysaccharides, organic acids, sterols, flavonoids, polyacetylenes, volatile oil and other nutritional components, such as vitamins, amino acids, carbohydrates, trace elements and the like, wherein the content of the ginsenoside and the polysaccharide is the highest, and the ginsenoside and the polysaccharide are the main active components of the American ginseng, so that the biological activity of the saponin is the highest. American ginseng can be used for treating cardiovascular diseases, enhancing immunity and inhibiting proliferation of cancer cells, and is once called "green gold". The total sugar content of American ginseng is about 68.84%, including polysaccharide, monosaccharide, oligosaccharide, etc., wherein the polysaccharide content is about 49.74%, which is one of the main active ingredients of American ginseng, and is divided into neutral polysaccharide and acidic polysaccharide according to the monosaccharide composition. Neutral polysaccharides mainly comprise glucose, galactose, arabinose, mannose, etc. The acidic polysaccharide is mainly composed of arabinose, glucose, galactose, galacturonic acid and glucuronic acid. The monosaccharide includes glucose, arabinose, rhamnose, fructose, sorbose, etc., and the oligosaccharide includes Ginseng radix trisaccharide, maltose, sucrose, etc. American ginseng has certain protection effect on cardiovascular system, and mainly has the effects of relieving myocardial ischemia reperfusion injury, resisting arrhythmia, protecting vascular endothelial cells and the like. In the study of the model of myocardial hypertrophy induced by using the angiotensin II immunosuppressant, american ginseng is found to remarkably reduce left ventricular dysfunction, inhibit blood pressure rise, eliminate myocardial cell hypertrophy reaction, and regulate CD36, CPT1M, glut4 and PDK4 gene expression induced by the angiotensin II immunosuppressant, which is possibly related to normalization of fatty acid and glucose oxidation. However, research on American ginseng polysaccharide is still insufficient, and development of American ginseng polysaccharide is limited by the extremely large molecular weight of American ginseng polysaccharide. In view of this, the use of fermentation means to enhance the activity of American ginseng polysaccharide is a major problem to be solved. Disclosure of Invention The invention aims to overcome the defects of the prior art and provide an American ginseng polysaccharide fermentation product with an anti-myocardial ischemia effect. The second object of the invention is to provide a preparation method of American ginseng polysaccharide ferment with the function of resisting myocardial ischemia. The third object of the invention is to provide an application of American ginseng polysaccharide ferment with the function of resisting myocardial ischemia in preparing medicaments for resisting myocardial ischemia. A preparation method of radix Panacis Quinquefolii polysaccharide fermented product with myocardial ischemia resisting effect comprises the following steps: 1) Heating and reflux-extracting crushed radix Panacis Quinquefolii with purified water for three times each for 1-2 hr, filtering, concentrating the filtrate under reduced pressure at 55-70deg.C to density of 0.9-1.05g/mL at 55-70deg.C to obtain concentrated solution, and adding purified water for three times which is 7-9, 5-7, and 5-7 times of radix Panacis Quinquefolii in sequence; 2) Mixing the concentrated solution with MRS culture medium containing lactobacillus plantarum according to the volume ratio of 96-98:4-2,