Search

CN-121975029-A - Wheat straw polysaccharide with activity of controlling sugar and lipid and preparation method thereof

CN121975029ACN 121975029 ACN121975029 ACN 121975029ACN-121975029-A

Abstract

The invention discloses wheat straw polysaccharide with glucose control and lipid regulation activities and a preparation method thereof, and belongs to the technical field of medicines. The method comprises the steps of (1) cleaning, cutting and crushing dried wheat straw to obtain wheat straw powder, (2) treating the wheat straw powder by using a pulse electric field, (3) adding hot water into the wheat straw powder treated in the step (2) to extract polysaccharide, and (4) adding high-temperature resistant alpha-amylase, cellulose and protease into the extract to carry out enzymolysis, centrifuging after the enzymolysis is finished, and dialyzing and freeze-drying supernatant to obtain the wheat straw polysaccharide. The invention takes the wheat straw polysaccharide as the raw material, can rapidly and efficiently separate and enrich the wheat straw polysaccharide with the activities of regulating blood fat, inhibiting alpha-amylase and high-grade saccharification end products, and expands the application of the product in terminal foods.

Inventors

  • QIAO XIAOJU
  • ZHU SHANBEN
  • WANG BEIBEI

Assignees

  • 善聚健康科技(江苏)有限公司

Dates

Publication Date
20260505
Application Date
20251231

Claims (8)

  1. 1. The preparation method of the wheat straw polysaccharide with the activity of controlling sugar and lipid is characterized by comprising the following steps: (1) Cleaning, cutting and crushing the wheat straw, and performing steam explosion treatment to obtain wheat straw powder; (2) Treating wheat straw powder with pulse electric field under the conditions of electric field frequency of 90-200Hz, pulse width of 6-20 μs and electric field strength of 5-20kv/cm; (3) Adding water into the wheat straw powder treated in the step (2) for extraction to obtain an extracting solution; (4) Adding high temperature resistant alpha-amylase, protease and cellulase into the extracting solution for step-by-step enzymolysis, centrifuging after the enzymolysis is finished, and dialyzing and drying supernatant to obtain wheat straw polysaccharide; The enzyme activity addition amount of the high temperature resistant alpha-amylase is 600-1500U/g substrate, the pH value of a reaction system is 5.8-6.5 when the high temperature resistant alpha-amylase is subjected to enzymolysis, the temperature is 45-90 ℃, and the enzymolysis time is 2-3 hours; the enzyme activity adding amount of the cellulase is 600-1500U/g of substrate, the pH value of a reaction system is 3-4.5 during enzymolysis of the cellulase, the temperature is 30-60 ℃, and the enzymolysis time is 2-3 hours; the enzyme activity adding amount of the neutral protease is 200-2000U/g substrate, the pH value of a reaction system is 6.0-8.0 when the neutral protease is subjected to enzymolysis, the temperature is 20-60 ℃, and the enzymolysis time is 2-4h.
  2. 2. The process according to claim 1, wherein the length of the cut segment in step (1) is 2 to 3cm and the mesh number after pulverization is 60 to 100 mesh.
  3. 3. The method according to claim 1, wherein the pressure of the steam explosion treatment in the step (1) is 2.5 to 3.5MPa and the dwell time is 60 to 120s.
  4. 4. The preparation method of claim 1, wherein the mass ratio of the wheat straw powder to the water in the step (3) is 1:3-6, the extraction temperature is 45-90 ℃ and the extraction time is 1-3 h.
  5. 5. The method according to claim 1, wherein the centrifugation in the step (4) is performed at 5000 to 10000 rpm for 5 to 20 minutes.
  6. 6. The method according to claim 1, wherein the condition of the dialysis in the step (4) is dialysis in pure water, the cut-off of the dialysis bag is 5000-10000 Da, and the dialysis time is 24-96 hours.
  7. 7. A straw polysaccharide, characterized in that it is produced according to the production method of any one of claims 1 to 6.
  8. 8. The use of the straw polysaccharide according to claim 7 in the fields of feed, pharmaceutical preparation, food or health products.

Description

Wheat straw polysaccharide with activity of controlling sugar and lipid and preparation method thereof Technical Field The invention relates to the technical field of medicines, in particular to wheat straw polysaccharide with glucose control and lipid regulation activities and a preparation method thereof. Background With the change of life style in modern society, the global incidence of diabetes and hyperlipidemia continues to rise, and has become a chronic metabolic disease that seriously threatens human health. Long-term hyperglycemia and hyperlipidemia can cause a series of complications such as cardiovascular and cerebrovascular diseases, nephropathy, neuropathy and the like. At present, although the clinical commonly used chemical synthesis hypoglycemic agents and lipid-regulating agents have remarkable curative effects, side effects such as liver and kidney function injury, gastrointestinal tract reaction and the like are often accompanied to different degrees. Therefore, the development of safe, efficient, low-toxic and side-effect bioactive substances from natural resources has become an important direction for the development of functional foods and medicines. Polysaccharides are important substances for energy storage and structural support of living beings as biological macromolecules widely existing in nature. Recent studies have shown that many natural polysaccharides derived from plants, fungi and algae have a variety of excellent biological activities including immunomodulation, antioxidation, antitumor, blood sugar regulation, blood lipid regulation, and the like. Its mechanism of action generally involves pathways that delay glucose absorption, improve insulin resistance, promote bile acid excretion, inhibit lipase activity, and the like. Natural polysaccharides are favored over chemical drugs for their good biocompatibility and safety. Wheat straw is a major agricultural byproduct after wheat harvest, and annual yield is huge. At present, most of wheat straws are not utilized in a high value except for being used for feed, papermaking or returning to the field, and the wheat straws are usually burnt or discarded, so that not only is the resource wasted, but also serious environmental pollution is caused. The main components of the wheat straw are cellulose, hemicellulose and lignin, and the wheat straw contains abundant structural polysaccharide. The polysaccharide has complex molecular structure and great potential of becoming bioactive polysaccharide. However, traditional utilization often neglects its profound bioactive value. Therefore, the development of an efficient, simple and convenient extraction method of the wheat straw polysaccharide with high purity and the exploration of the deep application of the wheat straw polysaccharide are of great significance for the progress of the medicine field. Disclosure of Invention The technical problems to be solved are as follows: aiming at the problems existing in the prior art, the invention provides wheat straw polysaccharide with the activity of controlling sugar and lipid and a preparation method thereof. The invention takes the dried wheat straw as the raw material, can quickly and efficiently separate and enrich the wheat straw polysaccharide with the activity of inhibiting alpha-amylase and lipase, and expands the application of the product in terminal foods. The technical scheme is as follows: the technical scheme of the invention is as follows: the first object of the present invention is to provide a preparation method of wheat straw polysaccharide, which comprises the following steps: (1) Cleaning, cutting and crushing the wheat straw, and performing steam explosion treatment to obtain wheat straw powder; (2) Treating the wheat straw powder using a pulsed electric field; (3) Adding water into the wheat straw powder treated in the step (2) for extraction to obtain an extracting solution; (4) Adding high temperature resistant alpha-amylase, protease and cellulase into the extracting solution for step-by-step enzymolysis, centrifuging after the enzymolysis is finished, and taking supernatant for dialysis and drying to obtain the wheat straw polysaccharide. In one embodiment of the invention, the wheat straw in step (1) is a straw obtained by post-treatment of harvested wheat, in particular yellow. In one embodiment of the invention, the length of the cut in step (1) is 2-3cm. In one embodiment of the present invention, the mesh number after pulverization in step (1) is 60 to 100 mesh. In one embodiment of the present invention, the pressure of the steam explosion treatment in the step (1) is 2.5 to 3.5MPa and the dwell time is 60 to 120s. In one embodiment of the present invention, the pulsed electric field treatment conditions in step (2) are an electric field frequency of 90-200Hz, a pulse width of 6-20 μs, and an electric field strength of 5-20kv/cm. In one embodiment of the invention, the mass ratio of the wheat straw powder t