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CN-121975032-A - Method for efficiently and environmentally extracting high-viscosity dendrobium devonianum polysaccharide

CN121975032ACN 121975032 ACN121975032 ACN 121975032ACN-121975032-A

Abstract

The invention discloses a method for efficiently and green extracting high-viscosity dendrobium devonianum polysaccharide, which belongs to the technical field of natural active substance extraction, and comprises the following steps of: pulverizing herba Dendrobii, defatting with ethanol, performing enzymolysis on the obtained residue in a buffer system, performing ultrasonic extraction on the enzymolysis solution, centrifuging to obtain supernatant, concentrating under reduced pressure, and sequentially performing alcohol precipitation, redissolution, purification and vacuum drying to obtain herba Dendrobii polysaccharide. The invention adopts a green process of synergistic effect of cellulase enzymolysis and ultrasonic auxiliary extraction, and remarkably improves the extraction efficiency and extraction rate of the dendrobium devonianum high-viscosity polysaccharide.

Inventors

  • CHEN CHUN
  • FU XIONG
  • ZHONG ZEWEN

Assignees

  • 华南理工大学

Dates

Publication Date
20260505
Application Date
20260130

Claims (10)

  1. 1. The method for extracting the high-viscosity dendrobium devonianum polysaccharide in a high-efficiency green manner is characterized by comprising the following steps of: Pulverizing herba Dendrobii, defatting with ethanol, performing enzymolysis on the obtained residue in a buffer system, performing ultrasonic extraction on the enzymolysis solution, centrifuging to obtain supernatant, concentrating under reduced pressure, and sequentially performing alcohol precipitation, redissolution, purification and vacuum drying to obtain herba Dendrobii polysaccharide.
  2. 2. The method for extracting high-viscosity dendrobium devonianum polysaccharide with high efficiency and greenness according to claim 1, wherein the degreasing treatment is to add the crushed dendrobium devonianum into 95vol% ethanol solution, shake and stir the mixture for 4 hours in a water bath at 70 ℃, and then centrifugally collect precipitate residues for drying.
  3. 3. The method for extracting high-viscosity dendrobium devonianum polysaccharide with high efficiency and green color according to claim 1, wherein the buffer system is acetate buffer with pH of 5.0.
  4. 4. The method for extracting high-viscosity dendrobium devonianum polysaccharide with high efficiency and greenness according to claim 1, wherein cellulase is used for enzymolysis, and the condition is that the feed liquid ratio of the residue to the buffer system is 1g to 50mL; the dosage of the cellulase is 666.67-1000U/g; the enzymolysis time is 60-75min.
  5. 5. The method for extracting high-viscosity dendrobium devonianum polysaccharide with high efficiency and green color as claimed in claim 4, wherein the dosage of the cellulase is 666.67-700U/g, and the enzymolysis time is 60-67min.
  6. 6. The method for extracting high-viscosity dendrobium devonianum polysaccharide with high efficiency and greenness according to claim 1, wherein the ultrasonic extraction condition is that the ultrasonic power is 80-200W and the ultrasonic temperature is 30-70 ℃.
  7. 7. The method for extracting high-viscosity dendrobium devonianum polysaccharide with high efficiency and green color as claimed in claim 6, wherein the ultrasonic power is 160-200W and the ultrasonic temperature is 50 ℃.
  8. 8. The method for extracting high-viscosity dendrobium devonianum polysaccharide with high efficiency and greenness as claimed in claim 1, wherein the condition of alcohol precipitation is that absolute ethanol with the volume being 4 times is added into the concentrated solution obtained by decompression concentration so that the ethanol concentration in the system is 80vol%, and the alcohol precipitation is carried out for 12 hours under the condition of 4 ℃.
  9. 9. The method for extracting high-viscosity dendrobium devoninum polysaccharide with high efficiency and green color according to claim 1, wherein the purification comprises a decoloring treatment and a deproteinizing treatment.
  10. 10. The method for extracting high-viscosity dendrobium devonianum polysaccharide with high efficiency and green color as claimed in claim 9, wherein the decoloring treatment is carried out by adding the crude polysaccharide solution obtained by re-dissolving into the pre-activated AB-8 macroporous resin, and standing for 12 hours; the deproteinization treatment is to add Sevag reagent into the polysaccharide solution after the decoloring treatment to remove protein in the solution.

Description

Method for efficiently and environmentally extracting high-viscosity dendrobium devonianum polysaccharide Technical Field The invention belongs to the technical field of natural active substance extraction, and particularly relates to a method for efficiently and environmentally extracting high-viscosity dendrobium devonianum polysaccharide. Background The dendrobium devoninum polysaccharide has similar effects to dendrobium candidum, but has more advantages in polysaccharide content, and has the core effects of enhancing immune regulation, benefiting stomach, promoting fluid production, resisting oxidation, resisting aging and the like, and has higher nutritive value. The dendrobium candidum occupies an important position in the local dietary culture of Yunnan, has extremely high food development potential, has higher cost performance compared with dendrobium candidum, is more easily accepted by mass consumer markets, and is an ideal raw material for deep-processing products (such as beverages, oral liquid and facial masks). The dendrobium polysaccharide is a main medicinal component of dendrobium plants, has higher content and viscosity, however, the traditional water extraction and alcohol precipitation method has obvious limitations, and the dendrobium polysaccharide needs to be finely crushed or ground, and can be extracted for a long time repeatedly by using a large amount of hot water. The traditional method has high energy consumption and long time consumption, and the problems of incomplete extraction and resource waste often occur in the extraction process. Therefore, it is important to explore a more efficient extraction method of dendrobe polysaccharide. Disclosure of Invention Aiming at the technical problems, the invention provides a method for efficiently and environmentally extracting high-viscosity dendrobium devonianum polysaccharide. According to the method, the dendrobium candidum polysaccharide is extracted by adopting an ultrasonic-assisted enzymolysis method, namely, the synergistic extraction combining cellulase enzymolysis and ultrasonic extraction is utilized, specific control and response surface optimization are carried out on specific parameters of enzymolysis and ultrasonic, and the optimal parameters are explored. In order to achieve the above purpose, the present invention provides the following technical solutions: a method for extracting high-viscosity dendrobium devonianum polysaccharide in a high-efficiency green manner comprises the following steps: Pulverizing herba Dendrobii, defatting with ethanol, performing enzymolysis on the obtained residue in a buffer system, performing ultrasonic extraction on the enzymolysis solution, centrifuging to obtain supernatant, concentrating under reduced pressure, and sequentially performing alcohol precipitation, redissolution, purification and vacuum drying to obtain herba Dendrobii polysaccharide. Alternatively, the degreasing treatment is to add the crushed dendrobium devonianum into 95% (vol) ethanol solution, shake and stir the mixture for 4 hours in a water bath at 70 ℃, and then centrifugally collect precipitate residues for drying. Further, the number of times of degreasing treatment is two. Alternatively, the buffer system is an acetate buffer at ph=5.0. Optionally, the enzymolysis condition is that the feed liquid ratio of the residue to the buffer system is 1:50 (g/mL); the dosage of the cellulase is 666.67-1000U/g; the enzymolysis time is 60-75min. Furthermore, the dosage of the cellulase is 666.67-700U/g, and the enzymolysis time is 60-67min. Optionally, the ultrasonic extraction condition is that the ultrasonic power is 80-200W and the ultrasonic temperature is 30-70 ℃. Further, the ultrasonic power is 160-200W, and the ultrasonic temperature is 50 ℃. Optionally, the condition of alcohol precipitation is that 4 times of volume of absolute ethyl alcohol is added into the concentrated solution obtained by decompression concentration to ensure that the concentration of the ethyl alcohol in the system is 80 percent, and the alcohol precipitation is carried out for 12 hours under the condition of 4 ℃. Optionally, the purifying includes a decolorizing treatment and a deproteinizing treatment. Further, the condition of the decoloring treatment is that the crude polysaccharide solution is added into the AB-8 macroporous resin which is pre-activated, and the mixture is kept stand for 12 hours. Further, the deproteinization treatment is to add Sevag reagent (chloroform: n-butanol volume ratio of 4:1) to the polysaccharide solution after the decoloring treatment to remove proteins in the solution. Compared with the prior art, the invention has the following advantages and technical effects: According to the invention, by adopting a green process of synergistic effect of cellulase enzymolysis and ultrasonic-assisted extraction, the extraction efficiency and the extraction rate of the dendrobium devonianum high-viscosity polysacchar