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CN-121975033-A - Rhizoma alpiniae oxyphyllae polysaccharide and preparation method and application thereof

CN121975033ACN 121975033 ACN121975033 ACN 121975033ACN-121975033-A

Abstract

The application relates to the technical field of biological medicine, in particular to alpinia galanga polysaccharide, a preparation method and application thereof. The alpinia galanga polysaccharide disclosed by the application can obviously reduce cough response of a model mouse by combining cigarette smoke exposure and LPS stimulation, can reverse the increase of lung coefficients, improve lung tissue injury and effectively relieve the degree of pulmonary edema, and has a good treatment effect on cough or diseases with cough symptoms. The preparation method of the alpinia oxyphylla polysaccharide is simple to operate, raw materials are easy to obtain, and the alpinia oxyphylla polysaccharide has the advantage of industrial mass production.

Inventors

  • XIANG JUAN
  • CHEN DONGFAN
  • TANG YUQING
  • YIN LIDAN
  • TANG YUREN
  • JIANG MENGYAO
  • CHE XINJIE
  • GONG ANQI

Assignees

  • 吉首大学

Dates

Publication Date
20260505
Application Date
20260210

Claims (14)

  1. 1. The preparation method of the alpinia galanga polysaccharide is characterized by comprising the following steps of: Mixing rhizoma Alpiniae Officinarum with water, decocting or extracting under reflux, concentrating the liquid, mixing the concentrated solution with alcohol, precipitating with ethanol, and collecting precipitate.
  2. 2. The preparation method according to claim 1, wherein the condition of the decoction comprises a feed liquid ratio of 1g (5 mL-30 mL), a decoction time of 0.5h-5h, a decoction time of 1-3 times, and/or a decoction time of 1-3 times; The conditions of heating reflux extraction include a feed-liquid ratio of 1g (5 mL-40 mL), a heating reflux time of 1h-2h, and a heating reflux time of 1-3 times.
  3. 3. The method according to claim 1 or 2, wherein the conditions of the alcohol precipitation include a volume ratio of the concentrate to the alcohol of 1 (3-5), an alcohol precipitation time of 6-48 hours, and an alcohol precipitation temperature of 0-30 ℃.
  4. 4. The method of manufacturing according to claim 1 or 2, further comprising the step of: deproteinizing the precipitated and redissolved polysaccharide solution to obtain deproteinized polysaccharide solution; decolorizing the deproteinized polysaccharide solution to obtain a decolorized polysaccharide solution; And (3) carrying out dialysis and desalination treatment on the decolored polysaccharide solution.
  5. 5. The method according to claim 4, wherein the step of deproteinizing the polysaccharide solution after precipitation and reconstitution comprises: Mixing the polysaccharide solution after precipitation and redissolution with protease for enzymolysis, and taking an enzymolysis solution; Optionally, before enzymolysis, regulating the polysaccharide solution after precipitation and redissolution to a mass concentration of 1mg/mL-30mg/mL; Optionally, the enzymolysis conditions comprise an enzyme bottom ratio of 1% -5%, an enzymolysis temperature of 30-70 ℃ and an enzymolysis time of 0.5-5 h; mixing the enzymolysis liquid with a protein precipitant, vibrating, and collecting a water layer; optionally, the volume ratio of the enzymolysis liquid to the protein precipitant is 1 (0.1-1), the oscillation time is 5-60min, and the repetition times are 1-5 times.
  6. 6. The method according to claim 4, wherein the deproteinized polysaccharide solution is decolorized by adsorption using a macroporous adsorbent resin: optionally, before adsorption, diluting the deproteinized polysaccharide solution to a mass concentration of 1mg/mL-30mg/mL; Optionally, the mass-to-volume ratio of the macroporous adsorption resin to the deproteinized polysaccharide solution is 1g (2 mL-20 mL); Optionally, the adsorption conditions comprise vibration adsorption at 100rpm-200rpm, adsorption temperature of 30-60 ℃ and adsorption time of 0.5-3 h.
  7. 7. The method according to claim 4, wherein the method for desalting the decolorized polysaccharide solution by dialysis comprises dialysis with a dialysis bag; optionally, the dialysis bag has a molecular weight cut-off of 3000Da to 7000Da.
  8. 8. A alpinia polysaccharide prepared by the preparation method of any one of claims 1 to 7.
  9. 9. The alpinia polysaccharide is characterized in that monosaccharide composition of the alpinia polysaccharide comprises glucose, galacturonic acid, galactose, arabinose, rhamnose, xylose, glucuronic acid, mannose and fucose; optionally, the alpinia galanga polysaccharide is prepared by the preparation method of any one of claims 1 to 7.
  10. 10. The alpinia polysaccharide according to claim 9, comprising 75.5 to 79.2% of the glucose, 8 to 9% of the galacturonic acid, 4 to 4.5% of the galactose, 3.8 to 4.2% of the arabinose, 2.5 to 3% of the rhamnose, 1 to 1.5% of the xylose, 0.8 to 1.2% of the glucuronic acid, 0.5 to 0.8% of the mannose and 0.2 to 0.3% of the fucose in mole percent.
  11. 11. The alpinia polysaccharide according to claim 9 or 10, wherein the alpinia polysaccharide has a number average molecular weight of 44kDa to 45kDa, a weight average molecular weight of 82kDa to 83kDa and a polydispersity index of 1.8 to 1.9.
  12. 12. Use of a alpinia oxyphylla polysaccharide for the preparation of a medicament for the treatment of cough or a disease with symptoms of cough, characterized in that the alpinia oxyphylla polysaccharide is according to claim 8 or according to any one of claims 9 to 11.
  13. 13. The use according to claim 12, wherein the medicament further comprises pharmaceutically acceptable excipients; Optionally, the pharmaceutically acceptable auxiliary materials comprise one or more of diluents, wetting agents, binders, disintegrants, lubricants, flavoring agents, solvents, solubilizers, cosolvents, emulsifiers, antioxidants, preservatives and pH regulators.
  14. 14. The use according to claim 12, wherein the medicament is in the form of a tablet, granule, pill, powder, capsule, injection, spray, aerosol, paste, syrup or oral liquid; alternatively, the route of administration of the drug includes oral administration, intravenous injection, inhalation administration, intraperitoneal injection, intramuscular injection, subcutaneous injection, sublingual administration, nasal administration or transdermal administration.

Description

Rhizoma alpiniae oxyphyllae polysaccharide and preparation method and application thereof Technical Field The application relates to the technical field of biological medicine, in particular to alpinia galanga polysaccharide, a preparation method and application thereof. Background Alpinia japonica (thunder.) Miq. Was originally in Ben Cao Jing Ji Zhi. Is mainly used for treating epigastric cold pain, cough due to cold lung, rheumatalgia, traumatic injury, menoxenia, fatigue and hematemesis, etc. Until now, only terpenes, flavonoids, volatile oils and other types of compounds have been reported in alpinia japonica, but polysaccharide components have not been elucidated. In recent years, plant polysaccharide is widely focused on the medicine and food industry because of biological activity and modification of food, and development of a preparation method and application of alpinia galanga polysaccharide has important significance in deep digging of the material basis of the national medicine and expansion of the resource utilization value of the national medicine. Cough is one of the most important defensive reflex of the airways, belonging to a protective physiological response, whose function is to clear respiratory secretions or foreign bodies, and to co-maintain airway cleanliness with the mucociliary transport system. However, when coughing is frequent or severe, it is converted into a symptom and causes a series of adverse effects such as respiratory muscle fatigue, sleep disturbance, urinary incontinence, delayed wound healing after operation, etc., which significantly reduce the physical activity and quality of life of the patient. Currently, clinically common antitussive drugs act mostly on the central nervous system, such as gabapentin, amitriptyline, pregabalin, and small doses of opioids (e.g. codeine and its analogues), which relieve abnormal cough responses mainly by inhibiting the cough centre or modulating nerve sensitization. Although these drugs can improve the quality of life of patients to some extent, long-term or general use may be accompanied by side effects such as sleepiness, constipation, dependency, and the like. Therefore, in recent years, the academy is working to find antitussive drugs which are safer and less in side effects. In this context, medicinal plants are receiving extensive attention for their potential antitussive activity and good safety, and are an important source for developing novel peripheral or central antitussive formulations. Disclosure of Invention Based on the above, one or more embodiments of the application provide a alpinia galanga polysaccharide, a preparation method and application thereof, and the alpinia galanga polysaccharide can effectively relieve cough symptoms and has good treatment effect on diseases with cough symptoms. The first aspect of the application provides a preparation method of alpinia galanga polysaccharide, comprising the following steps: Mixing rhizoma Alpiniae Officinarum with water, decocting or extracting under reflux, concentrating the liquid, mixing the concentrated solution with alcohol, precipitating with ethanol, and collecting precipitate. In some embodiments, the conditions of the decoction include a feed to liquid ratio of 1g (5 mL-30 mL), a decoction time of 0.5h-5h, a decoction time of 1-3 times, and/or a decoction time of 0.5h-5h; The conditions of heating reflux extraction include a feed-liquid ratio of 1g (5 mL-40 mL), a heating reflux time of 1h-2h, and a heating reflux time of 1-3 times. In some embodiments, the conditions for the alcohol precipitation include a volume ratio of the concentrate to the alcohol of 1 (3-5), an alcohol precipitation time of 6-48 hours, and an alcohol precipitation temperature of 0-30 ℃. In some embodiments, the preparation method further comprises the steps of: deproteinizing the precipitated and redissolved polysaccharide solution to obtain deproteinized polysaccharide solution; decolorizing the deproteinized polysaccharide solution to obtain a decolorized polysaccharide solution; And (3) carrying out dialysis and desalination treatment on the decolored polysaccharide solution. Further, the step of deproteinizing the polysaccharide solution after precipitation and reconstitution comprises: Mixing the polysaccharide solution after precipitation and redissolution with protease for enzymolysis, and taking an enzymolysis solution; Optionally, before enzymolysis, regulating the polysaccharide solution after precipitation and redissolution to a mass concentration of 1mg/mL-30mg/mL; Optionally, the enzymolysis conditions comprise an enzyme bottom ratio of 1% -5%, an enzymolysis temperature of 30-70 ℃ and an enzymolysis time of 0.5-5 h; mixing the enzymolysis liquid with a protein precipitant, vibrating, and collecting a water layer; optionally, the volume ratio of the enzymolysis liquid to the protein precipitant is 1 (0.1-1), the oscillation time is 5-60min, and the repetition times are 1-