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CN-121975038-A - Method for preparing high-molecular high-light-transmittance tamarind polysaccharide by using non-organic solvent

CN121975038ACN 121975038 ACN121975038 ACN 121975038ACN-121975038-A

Abstract

The invention discloses a method for preparing high molecular and high light-transmitting tamarind polysaccharide by using a non-organic solvent. The method comprises the steps of carrying out stabilization pretreatment on tamarind kernel extract, adding a biodegradable chelating agent EDDS and an antioxidant OTA, and combining step-by-step enzymolysis and refining processes of amylase and compound protease. The invention avoids organic solvent residue and acid extraction degradation, and the obtained product has high molecular weight, extremely low protein residue and excellent transmittance, and has wide application prospect in the fields of cosmetics and the like.

Inventors

  • XU JINRONG
  • YANG FEIFEI
  • SHENG XUE
  • LI DONGFANG

Assignees

  • 点滴(南京)生物科技有限公司

Dates

Publication Date
20260505
Application Date
20260403

Claims (9)

  1. 1. The method for preparing the high-molecular high-light-transmittance tamarind polysaccharide by using the non-organic solvent is characterized by comprising the following steps of: Extracting tamarind kernels to obtain an extracting solution; Subjecting the extract to a stabilization pretreatment, wherein the stabilization pretreatment comprises adding a biodegradable chelating agent and an antioxidant; Carrying out enzymolysis on the pretreated extracting solution, wherein the enzymolysis comprises amylase enzymolysis and compound protease enzymolysis; refining and drying the enzymatic hydrolysate after enzymatic hydrolysis to obtain the tamarind polysaccharide.
  2. 2. The method according to claim 1, wherein the extraction step comprises pulverizing shelled tamarind kernels, sieving to obtain kernel powder, soaking in deionized water, and heating for extraction at 100deg.C.
  3. 3. The method of claim 1, wherein the biodegradable chelating agent is ethylenediamine disuccinic acid and the antioxidant is an oxo-thiazolidine hydroxy acid.
  4. 4. The method according to claim 1, wherein the enzymolysis step comprises enzymolysis by a compound protease and enzymolysis by an amylase, the enzymolysis temperature of the compound protease is 45-55 ℃, the enzymolysis time is 1-3h, the enzymolysis temperature of the amylase enzymolysis is 60-70 ℃, and the enzymolysis time is 1-3h.
  5. 5. The method of claim 4, wherein the complex protease comprises a protease and a papain, and the amylase is a mesophilic alpha-amylase.
  6. 6. The method according to claim 5, wherein the enzymatic hydrolysis step comprises specifically adding 0.05 wt% of a medium temperature alpha-amylase at 60 ℃ for 1h, followed by adding 0.05 wt% papain and 0.05 wt% protease at 55 ℃ for 1h.
  7. 7. The method according to claim 1, wherein the refining step comprises enzyme deactivation, activated carbon decolorization, filtration and ultrafiltration, wherein the enzyme deactivation is carried out at 90 ℃ for 30min, the decolorization is carried out at 80 ℃ by adding 1wt% of activated carbon and stirring for 1h, the filtration is carried out by plate frame filtration and diatomite filtration is added, and the ultrafiltration is carried out by a membrane with a molecular weight cutoff of 1 ten thousand, and a reflux liquid is received.
  8. 8. The method according to claim 1, wherein the drying step comprises sterilizing the reflux liquid at 80±2 ℃ for 30min, and then vacuum drying and pulverizing at low temperature.
  9. 9. A tamarind seed polysaccharide prepared by the process of any one of claims 1-8.

Description

Method for preparing high-molecular high-light-transmittance tamarind polysaccharide by using non-organic solvent Technical Field The invention relates to the technical field of tamarind polysaccharide, in particular to a method for preparing high molecular and high light transmittance tamarind polysaccharide by using a non-organic solvent. Background The tamarind kernel contains 65-73% of non-fibrous carbohydrate, 15-20% of protein, 6-8% of fat, 3-5% of fiber and 2.5-3.2% of ash by mass. The non-cellulose carbohydrate comprises starch and tamarind polysaccharide, the starch comprises amylose and amylopectin, the starch is of a non-branched spiral structure, the starch is formed by connecting 24-30 glucose residues end to end through alpha-1, 4-glycosidic bonds, and the branched chain is alpha-1, 6-glycosidic bonds. The amylose has better water solubility, can be dissolved in cold water, and the amylopectin can be gradually dissolved under the heating condition to form a viscous solution although the amylopectin is insoluble in cold water. The molecular structure of tamarind polysaccharide is that the main chain is glucose connected with beta-D1, 4 glycosidic bond, the side chain is xylose connected with alpha-D-1, 6 glycosidic bond and galactose connected with beta-D-1, 2 glycosidic bond, and the molecular weight is 720-880 kDa. The kernel proteins are mainly globulins and albumins, and the amino acid composition is found to be very rich in all six other essential amino acids, especially lysine, phenylalanine and leucine, except threonine and tyrosine. At present, tamarind polysaccharide is widely used in the food field, but has certain limitation in the use in the cosmetic field. Mainly because organic solvents are used for extracting tamarind polysaccharide, for example, chinese patent (CN 104844809 a) discloses a method for preparing tamarind polysaccharide gel, in which isopropanol or ethanol is used to precipitate polysaccharide, and a problem of small amount of organic solvent residue is also proposed, and the organic solvent residue in cosmetics affects skin safety. At present, most of tamarind polysaccharide extraction uses organic reagents, and the organic reagents are not thoroughly removed, so that opaque matters are formed with the polysaccharide, and the solution is turbid. In addition, the organic reagent may precipitate together with fat-soluble impurities (oils, pigments, etc.), which precipitate together with the polysaccharide to form light scattering particles, reducing transmittance. Organic solvent precipitation has a certain selectivity for impurity removal, but cannot completely remove structurally similar water-soluble impurities (e.g., oligosaccharides, monosaccharides, certain proteins). In addition, the solvent residue itself is also an "impurity" affecting the purity of the polysaccharide. Organic solvent precipitation generally does not directly degrade polysaccharide chains, but if a high temperature, strong acid step is present during extraction, molecular chain cleavage may result, and molecular weight is reduced. The same way of acid extraction and alcohol precipitation is the more extraction method used at present, but low pH can cause the molecular weight of tamarind polysaccharide to be reduced, which can influence the skin feel feeling of the skin. Yet another reason for affecting the use in cosmetics is protein residues, although chinese patent (CN 107501424 a) discloses a method for purifying tamarind gum polysaccharide, in which papain is used to reduce the protein in the polysaccharide, the protein amino acid composition of the kernel is complex, papain belongs to thiol protease, and tends to hydrolyze specific amino acids (such as arginine, lysine, phenylalanine, etc.), which also makes the hydrolysis impossible. A common phenomenon brought by the above methods is the problem of light transmittance, and the clear and transparent polysaccharide solution is easily applied to various dosage forms of products and is also easily accepted by consumers, but the light transmittance of tamarind polysaccharide on the market is not ideal at present. These problems have greatly hindered the use of tamarind polysaccharides in cosmetics. Disclosure of Invention The application provides a method for preparing high molecular and high light transmittance tamarind polysaccharide by using a non-organic solvent, which comprises the following steps: Extracting tamarind kernels to obtain an extracting solution; Subjecting the extract to a stabilization pretreatment, wherein the stabilization pretreatment comprises adding a biodegradable chelating agent and an antioxidant; Carrying out enzymolysis on the pretreated extracting solution, wherein the enzymolysis comprises amylase enzymolysis and compound protease enzymolysis; refining and drying the enzymatic hydrolysate after enzymatic hydrolysis to obtain the tamarind polysaccharide. The method realizes the preparation of the tamarind pol