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CN-121975150-A - Preparation method of hydrogel based on ice skeleton

CN121975150ACN 121975150 ACN121975150 ACN 121975150ACN-121975150-A

Abstract

The application relates to a hydrogel based on an ice skeleton and a preparation method thereof, wherein the ice skeleton is adopted as a template for guiding, 30wt% PVA solution is poured and filled into the ice skeleton at the temperature of-4 ℃ to-6 ℃, the ice skeleton is frozen at the temperature of-18 ℃ to-22 ℃, and the ice skeleton is removed by dissolution at the temperature of 20 ℃ to 30 ℃ to obtain the hydrogel with a preset three-dimensional directional microstructure. According to the application, by taking the ice skeleton as a template guide, PVA solution filling, freezing setting and ice melting demolding process route, the obtained hydrogel has a regular three-dimensional directional microstructure, the mechanical strength and the structural stability are superior to those of random porous hydrogel, the technical pain points of biotoxicity residue/difficult demolding faced in the traditional method are solved, and the precise regulation and control of the high biocompatibility, high integrity, orientation and size of the hydrogel are realized.

Inventors

  • LU QIFENG
  • ZHANG MINGXUAN
  • LIU YIMING
  • ZHAO YINCHAO
  • CHEN WEI

Assignees

  • 西交利物浦大学

Dates

Publication Date
20260505
Application Date
20260214

Claims (10)

  1. 1. A method for preparing hydrogel based on an ice skeleton, which is characterized by comprising the following steps: S1, firstly preparing an ice skeleton matched with a preset three-dimensional directional microstructure; s2, pouring and filling 30wt% of PVA solution into the ice skeleton in the step S1 at the temperature of-4 ℃ to-6 ℃, freezing at the temperature of-18 ℃ to-22 ℃, and dissolving and removing the ice skeleton at the temperature of 20 ℃ to 30 ℃ to obtain the hydrogel with the preset three-dimensional directional microstructure.
  2. 2. The method of preparing as claimed in claim 1, wherein the ice skeleton is prepared by the steps of: Preparing a three-dimensional solid template with a preset three-dimensional directional microstructure by using polybutylene succinate, immersing the template in deionized water, pre-freezing at-4 ℃ to-6 ℃ to form an ice skeleton inside the three-dimensional solid template, transferring the ice skeleton into a dichloromethane solution for soaking, and dissolving and removing the polybutylene succinate at-18 ℃ to-22 ℃ to obtain the ice skeleton.
  3. 3. The method of claim 2, wherein the three-dimensional solid template is obtained by 3D printing.
  4. 4. The method of claim 2, wherein the predetermined three-dimensional directional microstructure comprises microchannels having a diameter >100 μm.
  5. 5. The preparation method of claim 2, wherein the liquid level of the deionized water is 1 cm-2 cm higher than the upper surface of the three-dimensional solid template, and the soaking time of the three-dimensional solid template in the deionized water is 2-4 hours.
  6. 6. The method according to claim 2, wherein the pre-freezing time is 4-6 hours.
  7. 7. The preparation method according to claim 2, wherein the soaking time is 6-12 hours, and the volume of the dichloromethane solution is 3-5 times that of the three-dimensional solid template.
  8. 8. The preparation method of the ice skeleton according to claim 2, further comprising the step of sterilizing the ice skeleton after dissolving and removing the polybutylene succinate to obtain the ice skeleton, wherein the ice skeleton is soaked in a 75wt% alcohol solution at-18 ℃ to-22 ℃ for 1-2 hours, and then residual alcohol on the surface is drained.
  9. 9. The method according to claim 1, wherein the freezing time is 8-24 hours.
  10. 10. A hydrogel having a predetermined three-dimensional directional microstructure obtained by the production method according to any one of claims 1 to 9.

Description

Preparation method of hydrogel based on ice skeleton Technical Field The invention relates to the technical field of hydrogels, in particular to a preparation method of a hydrogel based on an ice skeleton. Background The microstructure hydrogel has wide application prospect in the fields of implantable tissue scaffolds, skin-attached flexible bioelectronics, flexible biosensing and the like due to soft mechanical properties and controllable porous structures, and the performance of the microstructure hydrogel is closely related to the morphology, orientation and system cleanliness of the pore canal. At present, a way of constructing the microstructure hydrogel is a sacrificial template technology, wherein the main flow of the sacrificial template technology is to firstly prepare a template, then prepare the microstructure hydrogel based on the template, and finally remove the template, however, the traditional template material mainly comprises paraffin, high polymer materials and the like, the materials are difficult to completely remove, small molecular impurities or particle fragments are easy to remain in the hydrogel system after treatment, and the residual substances are extremely easy to cause adverse interface reactions in application scenes with strict requirements on biocompatibility, so that the safe application of the hydrogel in the relevant fields of biomedicine is severely limited. The second way of constructing the microstructure hydrogel is to dope pore-forming agent (including salt particles, sugar particles, etc.) and obtain porous structure by forming voids inside the hydrogel, but the method of doping pore-forming agent can not realize accurate regulation and control of pore channels, and the formed internal pore channels are in random distribution state, so that the morphology, orientation and size of the internal pore channels are difficult to match with the functional requirements of specific application, and the structure and performance of the microstructure hydrogel can not be effectively adapted to the use requirements of a target scene. Therefore, how to construct the microstructure hydrogel with high biocompatibility and functional suitability becomes a key technical problem to be broken through in the field on the premise of avoiding chemical residues, improving biocompatibility and structural damage and realizing the precise controllability of the shape, orientation and size of the pore canal. Disclosure of Invention In order to solve the technical problems, the application provides a preparation method of hydrogel based on an ice skeleton. In order to achieve the above purpose, the present application is realized by the following technical scheme: the application provides a preparation method of hydrogel based on an ice skeleton, which comprises the following steps: S1, firstly preparing an ice skeleton matched with a preset three-dimensional directional microstructure; s2, pouring and filling 30wt% of PVA solution into the ice skeleton in the step S1 at the temperature of-4 ℃ to-6 ℃, freezing at the temperature of-18 ℃ to-22 ℃, and dissolving and removing the ice skeleton at the temperature of 20 ℃ to 30 ℃ to obtain the hydrogel with the preset three-dimensional directional microstructure. As a further improvement of the present application, the ice skeleton is prepared by the steps of: Preparing a three-dimensional solid template with a preset three-dimensional directional microstructure by using polybutylene succinate, immersing the template in deionized water, pre-freezing at-4 ℃ to-6 ℃ to form an ice skeleton inside the three-dimensional solid template, transferring the ice skeleton into a dichloromethane solution for soaking, and dissolving and removing the polybutylene succinate at-18 ℃ to-22 ℃ to obtain the ice skeleton. As a further improvement of the application, the three-dimensional entity template is obtained by adopting 3D printing. As a further development of the application, the predetermined three-dimensional directional microstructure comprises microchannels with a diameter >100 μm. As a further improvement of the application, the liquid level of the deionized water is 1 cm-2 cm higher than the upper surface of the three-dimensional solid template, and the soaking time of the three-dimensional solid template in the deionized water is 2-4 hours. As a further improvement of the application, the pre-freezing time is 4-6 hours. As a further improvement of the application, the soaking time is 6-12 hours, and the volume of the dichloromethane solution is 3-5 times of that of the three-dimensional solid template. As a further improvement of the application, after the polybutylene succinate is dissolved and removed to obtain the ice skeleton, the method further comprises the step of sterilizing the ice skeleton, namely, soaking the ice skeleton in a 75wt% alcohol solution at the temperature of-18 ℃ to-22 ℃ for 1-2 h, and then draining residual alcohol on the surfac