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CN-121975158-A - Carrier for realizing long-acting controlled release of cinnamaldehyde based on synergism of bacterial cellulose and polylactic acid, preparation method thereof and application thereof in fruit and vegetable fresh-keeping

CN121975158ACN 121975158 ACN121975158 ACN 121975158ACN-121975158-A

Abstract

The invention relates to a carrier for realizing long-acting controlled release of cinnamaldehyde based on synergism of bacterial cellulose and polylactic acid, a preparation method thereof and application thereof in fruit and vegetable fresh-keeping, wherein the preparation method comprises the steps of firstly placing the bacterial cellulose in a eutectic solvent system containing citric acid, glycerol and choline chloride, and heating for reaction to obtain CABC; and placing the CABC in an activation system containing 1-ethyl-3- (3-dimethylaminopropyl) carbodiimide and N-hydroxysuccinimide to obtain CABC-ONH 2 , performing oximation reaction on the CABC-ONH 2 and cinnamaldehyde to obtain CABC/CIN, and finally mixing the CABC/CIN and polylactic acid to form a film to obtain the bacterial cellulose/polylactic acid synergistic cinnamaldehyde long-acting controlled release carrier. Compared with the prior art, the preparation method effectively reduces the initial burst release of CIN in the degradable carrier, improves the stability and activity retention rate of the release in the middle and later stages, improves the cooperative processing suitability with PLA, and simultaneously realizes the antibacterial and antioxidant effects in the fruit and vegetable fresh-keeping application.

Inventors

  • YUAN HAIBIN
  • DONG WEI
  • WU QIWEI
  • Yue Zhigun
  • CHEN CHEN
  • YU HAIYAN
  • TIAN HUAIXIANG

Assignees

  • 上海应用技术大学

Dates

Publication Date
20260505
Application Date
20260129

Claims (10)

  1. 1. The preparation method of the carrier for realizing the long-acting controlled release of the cinnamaldehyde based on the cooperation of the bacterial cellulose and the polylactic acid is characterized by comprising the following steps of: s1, placing bacterial cellulose in a eutectic solvent system containing citric acid, glycerol and choline chloride, and heating for reaction to obtain CABC; S2, placing the CABC in an activation system containing 1-ethyl-3- (3-dimethylaminopropyl) carbodiimide and N-hydroxysuccinimide to obtain CABC-ONH 2 ; S3, performing oximation reaction on the CABC-ONH 2 and cinnamaldehyde to obtain CABC/CIN; and S4, mixing CABC/CIN with polylactic acid to form a film, and obtaining the bacterial cellulose/polylactic acid synergistic cinnamaldehyde long-acting controlled release carrier.
  2. 2. The preparation method of the carrier for realizing the long-acting controlled release of the cinnamaldehyde based on the cooperation of the bacterial cellulose and the polylactic acid, which is disclosed in the claim 1, is characterized in that in the step S1, the molar ratio of the bacterial cellulose, the citric acid, the glycerol and the choline chloride is (8-12): 1 (2-8): 1-3.
  3. 3. The preparation method of the carrier for realizing the long-acting controlled release of the cinnamaldehyde based on the cooperation of the bacterial cellulose and the polylactic acid, which is disclosed in the claim 1, is characterized in that in the step S1, the reaction temperature is 80-120 ℃ and the reaction time is 30-120 min.
  4. 4. The method for preparing the carrier for realizing the long-acting controlled release of the cinnamaldehyde based on the synergy of the bacterial cellulose and the polylactic acid, which is disclosed in the claim 1, is characterized in that in the step S2, the concentration of the 1-ethyl-3- (3-dimethylaminopropyl) carbodiimide in the activation system is 0.2-0.6mol.L -1 , the concentration of the N-hydroxysuccinimide is 0.2-0.6mol.L -1 , and the content of the CABC in the activation system is 0.5-1.0wt%.
  5. 5. The preparation method of the carrier for realizing the long-acting controlled release of cinnamaldehyde based on the cooperation of bacterial cellulose and polylactic acid, which is disclosed in claim 1, is characterized in that the step S2 comprises the step of standing CABC in an activation system at 25-30 ℃ for 1-2 h to obtain CABC-ONH 2 .
  6. 6. The preparation method of the carrier for realizing the long-acting controlled release of the cinnamaldehyde based on the cooperation of the bacterial cellulose and the polylactic acid, which is disclosed in claim 1, is characterized in that in the step S3, in the oximation reaction, the pH is 5.0-5.8, the reaction temperature is 20-40 ℃, the reaction time is 1-3 hours, and the mass ratio of the cinnamaldehyde to the CABC-ONH 2 is 0.5:1-1.5:1.
  7. 7. The method for preparing the carrier for realizing the long-acting controlled release of the cinnamaldehyde based on the synergy of the bacterial cellulose and the polylactic acid, which is disclosed in the claim 1, is characterized in that in the step S4, the film forming comprises the steps of blending and extruding CABC/CIN with PLA according to the weight percentage of 1-10 to form a film with the thickness of 20-80 mu m, or preparing the CABC/CIN into a dispersion liquid, coating and drying the dispersion liquid on a PLA substrate.
  8. 8. A carrier for realizing the long-acting controlled release of cinnamaldehyde based on the cooperation of bacterial cellulose and polylactic acid, which is characterized in that the carrier is prepared by adopting the method of any one of claims 1 to 7.
  9. 9. The application of the carrier for realizing the long-acting controlled release of the cinnamaldehyde based on the cooperation of the bacterial cellulose and the polylactic acid according to claim 8, which is characterized in that the long-acting controlled release carrier of the cinnamaldehyde based on the cooperation of the bacterial cellulose and the polylactic acid is used for preserving fruits and vegetables.
  10. 10. The application of the carrier for realizing the long-acting controlled release of the cinnamaldehyde based on the cooperation of the bacterial cellulose and the polylactic acid, which is disclosed in claim 9, is characterized in that the fruits and vegetables are respiratory transition fruits and vegetables, and the fresh-keeping environment comprises 2-4% of oxygen and 3-7% of carbon dioxide by volume.

Description

Carrier for realizing long-acting controlled release of cinnamaldehyde based on synergism of bacterial cellulose and polylactic acid, preparation method thereof and application thereof in fruit and vegetable fresh-keeping Technical Field The invention belongs to the technical field of green packaging and active matter controlled release, and relates to a carrier for realizing long-acting controlled release of cinnamaldehyde based on cooperation of bacterial cellulose and polylactic acid, a preparation method thereof and application thereof in fruit and vegetable fresh-keeping. Background Cinnamaldehyde (CINNAMALDEHYDE, CIN for short) has broad-spectrum inhibition activity on gram-negative and gram-positive bacteria, has free radical scavenging capability, and is suitable for quality maintenance of fresh fruits and vegetables. However, CIN is highly volatile and easily oxidized, and a release profile of rapid dissipation of the initial burst is easily developed in a common polymer matrix, while its hydrophobicity and reactivity lead to insufficient compatibility with degradable polylactic acid (PLA), which is prone to migration loss and performance fluctuations during film formation processing. In the prior art, physical adsorption, plasticization blending or simple microencapsulation is mostly adopted to fix CIN, for example, patent CN120588601A discloses a polylactic acid antibacterial packaging film and a preparation method, raw materials comprise polylactic acid, polyvinyl alcohol, nano zinc oxide, tea tree essential oil-eugenol composite antibacterial agent, corn starch, carboxymethyl chitosan, dynamic disulfide bond crosslinking agent and the like, and the preparation is completed through electrostatic spinning compounding after a base film is prepared by melt blending and a corn starch embedding antibacterial agent is prepared. The corn starch embedded microcapsule has the defects that the initial burst release is high, the initial structural stability of the corn starch embedded microcapsule is insufficient, a starch chain is quickly swelled and the porosity is suddenly increased after the corn starch embedded microcapsule is contacted with a simulated liquid, an antibacterial agent is quickly released, the release of 35.2 mug/cm 2 is implemented in 124h, the non-fully embedded comparative example 3 reaches 68.3 mug/cm 2, the weakness is released in the later stage, after the swelling of a starch embedded network is stable, the diffusion resistance of the antibacterial agent is increased, part of the antibacterial agent and starch form firm hydrogen bonds, the antibacterial agent is difficult to continuously release, and the long-acting antibacterial effect is influenced. The patent CN114081869A discloses a preparation method, a product and an application of microcapsule inlaid chitosan solid perfume, wherein polylactic acid is used as a wall material and plant essential oil is used as a core material, the microcapsule is prepared by a double emulsion method, then chitosan solution is mixed into the microcapsule, freeze-dried into foam, and finally the microcapsule is immersed with the essential oil, so that the microcapsule has the defects that the polylactic acid wall material is insufficient in thermal stability, the microcapsule is easy to crack in the scenes of melting preparation of cosmetics, high-temperature mixing of coating and the like, the essential oil is easy to burst or migrate due to poor compatibility with the matrix, a chitosan foam pore canal is easy to be influenced by environmental humidity, the microcapsule lacks a dynamic regulation mechanism, and long-term release is difficult to be controlled stably. Patent CN120737567A discloses a degradable bio-based food packaging material and a preparation method thereof, and the components comprise polylactic acid, ITA esterified bacterial cellulose, CSL-ITA esterified bacterial cellulose and the like, and the degradable bio-based food packaging material is prepared by esterification grafting, gradient dehydration, supercritical CO 2 drying, melt blending and photo-crosslinking. Although the compatibility of components is optimized, a site capable of forming a reversible chemical bond with an antibacterial substance is not introduced into the modified bacterial cellulose, the antibacterial component is still mainly physically adsorbed, is easy to release rapidly in the initial stage of use, has the problem of burst release, and is difficult to meet the long-acting antibacterial requirement of food packaging. Disclosure of Invention The invention aims to provide a carrier for realizing long-acting controlled release of cinnamaldehyde based on synergy of bacterial cellulose and polylactic acid, a preparation method thereof and application thereof in fruit and vegetable fresh-keeping, which are used for reducing initial burst release of CIN in a degradable carrier, improving middle and later release stability and activity retention rate, im