CN-121975162-A - Preparation method and application of chlorogenic acid-zein nanoparticle-loaded chitosan film

Abstract

The invention discloses a preparation method and application of a chlorogenic acid-zein nanoparticle-loaded chitosan film, and belongs to the technical field of food packaging. The method comprises the steps of firstly preparing chlorogenic acid-zein nano particles by taking zein as a wall material through an anti-solvent precipitation method, then blending the chlorogenic acid-zein nano particles with chitosan film-forming solution, and performing tape casting film formation to prepare a composite film, wherein the technical problems of easiness in oxidative degradation of chlorogenic acid, poor water solubility, insufficient mechanical property and single function of a pure chitosan film are solved through regulating and controlling the nano particle preparation process and film composite parameters. The prepared composite film has excellent barrier property, mechanical property, thermal property and oxidation resistance, and can effectively delay the oxidative deterioration of millet in the storage period.

Inventors

• ZHANG LIZHEN
• ZHANG HUANHUAN
• WANG NUO
• ZHAO SHAOJIE
• XING BAO
• YANG PU

Assignees

• 山西大学

Dates

Publication Date

20260505

Application Date

20260303

Claims (6)

1. 1. The preparation method of the chlorogenic acid-zein nanoparticle-loaded chitosan film is characterized by comprising the following steps of: (1) The preparation of chlorogenic acid-zein nano-particles comprises the steps of dissolving zein and chlorogenic acid in ethanol water solution for 30min to prepare zein and chlorogenic acid solution, mixing the prepared zein and chlorogenic acid solution, stirring and dissolving for 1h in a dark place, adding a certain volume of deionized water into the mixed solution, continuously stirring for 1h, and removing ethanol by rotary evaporation to prepare the chlorogenic acid-loaded zein nano-particle suspension. (2) The preparation of the chitosan film forming liquid comprises the steps of dissolving chitosan in acetic acid solution, adding glycerol, heating and stirring for 30min until the chitosan film forming liquid is completely dissolved, and cooling to room temperature to obtain the chitosan film forming liquid. (3) The preparation of the composite film comprises the steps of dispersing chlorogenic acid-zein nano particles obtained in the step (1) in the chitosan film forming liquid obtained in the step (2), stirring for 30min in a dark place, pouring the mixture into a mold after ultrasonic degassing for 30min, and drying the mixture in a 45 ℃ oven for 12 hours to obtain the chlorogenic acid-zein nano particle-loaded chitosan film.
2. 2. The method for preparing the chlorogenic acid-zein nanoparticle-loaded chitosan film according to claim 1, wherein the mass ratio of zein to chlorogenic acid in the step (1) is (1-10): 1, the volume fraction of the aqueous ethanol solution in the step (1) is 80%, and the volume ratio of deionized water to the mixed solution is 5:1.
3. 3. The method for preparing a chlorogenic acid-zein nanoparticle-loaded chitosan film according to claim 1, wherein the volume fraction of the acetic acid solution in the step (2) is 1%, the glycerol is added in an amount of 50% of the mass of chitosan, and the heating temperature is 65 ℃.
4. 4. The method for preparing a chlorogenic acid-zein nanoparticle-loaded chitosan film according to claim 1, wherein the volume ratio of the nanoparticle to the chitosan film-forming solution in the step (3) is (0.02-0.11): 1.
5. 5. A method for preparing a chitosan film loaded with chlorogenic acid-zein nano-particles, which is characterized in that the chitosan film of chlorogenic acid-zein nano-particles prepared by the method according to any one of claims 1 to 4.
6. 6. The chlorogenic acid-zein nanoparticle-loaded chitosan film of claim 5 is effective to delay oxidative deterioration during storage of millet.

Description

Preparation method and application of chlorogenic acid-zein nanoparticle-loaded chitosan film Technical Field The invention belongs to the technical field of food packaging, and relates to a preparation method and application of a chlorogenic acid-zein nanoparticle-loaded chitosan film. Background With the increasing awareness of consumers on food safety quality and environmental protection, the development of novel food packaging materials which are biodegradable and have the function of delaying food deterioration has become a research hotspot in the fields of food science and material engineering. The traditional petroleum-based plastic package is difficult to degrade, causes serious white pollution, has single function, and cannot inhibit microbial spoilage and oxidative deterioration of foods in the storage process. Therefore, the packaging film with the functions of biodegradability and food deterioration delay is an ideal solution for replacing the traditional plastic and improving the shelf life of the food. As natural aminopolysaccharide, chitosan (CS) has good film forming property, biocompatibility, degradability and certain antibacterial property, is an ideal base material for food packaging, but the pure chitosan film has the defects of insufficient mechanical property, limited barrier property and single function, and limits the application of the pure chitosan film. In order to impart antioxidant and antibacterial activities to packaging films, natural polyphenol compounds such as chlorogenic acid (CGA) are often added to the film matrix. Chlorogenic acid is a natural polyphenol active ingredient and has excellent antioxidant and antibacterial activities, but has extremely unstable chemical properties, is easily subjected to oxidative degradation under the influence of light and heat, has poor water solubility and low bioavailability, and is difficult to realize long-acting and stable activity exertion when being directly added into a film. Zein (Zein) is a natural plant protein, has good biocompatibility, degradability and hydrophobicity, is an ideal wall material for encapsulating hydrophobic/amphiphilic active ingredients, can be prepared into nano particles by an anti-solvent precipitation method to physically protect the active ingredients and improve the stability of the active ingredients, but no report on the preparation of functional films by compounding chlorogenic acid-Zein nano particles with chitosan exists at present. Therefore, it is necessary to develop a nanoparticle with natural zein as a wall material for encapsulating chlorogenic acid, and to prepare a functional film with high stability, high activity and excellent mechanical properties by compounding with chitosan, thereby helping to delay oxidative deterioration of millet in the storage period. Disclosure of Invention Aiming at the defects of the prior art, the primary aim of the invention is to provide a preparation method of a chitosan film loaded with chlorogenic acid-zein nano particles, which takes zein as a natural wall material to realize encapsulation and stabilization of chlorogenic acid and simultaneously improve the barrier property, mechanical property, thermal property and oxidation resistance of the chitosan film. The second object of the invention is to provide the application of the composite film in the storage period of millet and delay the oxidative deterioration of the millet in the storage period. In order to achieve the above purpose, the specific technical scheme of the invention is as follows: in a first aspect, the invention provides a method for preparing a chlorogenic acid-zein nanoparticle-loaded chitosan film, comprising the steps of: (1) The chlorogenic acid-zein nanoparticle is prepared by dissolving zein and chlorogenic acid in 80% ethanol water solution for 30min to obtain zein and chlorogenic acid solution, mixing zein and chlorogenic acid solution according to the mass ratio of (1-10) 1, stirring and dissolving in dark for 1 hr, adding deionized water with a certain volume, stirring for 1 hr, and steaming to remove ethanol to obtain chlorogenic acid loaded zein (2) The preparation of the chitosan film forming liquid comprises the steps of dissolving chitosan in acetic acid solution with the volume fraction of 1%, adding glycerol with the mass of 50% of chitosan, heating and stirring at 65 ℃ for 30min until the chitosan film forming liquid is completely dissolved, and cooling to room temperature to obtain the chitosan film forming liquid. (3) The preparation of the composite film comprises the steps of dispersing chlorogenic acid-zein nano particles obtained in the step (1) in the chitosan film forming liquid obtained in the step (2) according to the volume ratio (0.02-0.11) of the chlorogenic acid-zein nano particles to the chitosan film forming liquid, stirring for 30min in a dark place, pouring the mixture into a die after ultrasonic degassing for 30min, adopting a casting film formi