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CN-121975163-A - Intelligent pectase response type chitosan nano composite packaging film and preparation method and application thereof

CN121975163ACN 121975163 ACN121975163 ACN 121975163ACN-121975163-A

Abstract

The invention discloses an intelligent pectase response type chitosan nano composite packaging film and a preparation method and application thereof, and belongs to the technical field of preservative film materials. The preparation method comprises the steps of firstly, compounding pectin, nisin and anthocyanin to form ternary nano-composites (APN NPs), then mixing the suspension with chitosan solution according to a specific proportion, and carrying out ultrasonic treatment, casting and drying to obtain the composite membrane. The packaging film takes pectase secreted by refrigerated fish putrefying microorganisms as a target biological switch, and triggers the on-demand release of nisin (antibacterial) and anthocyanin (antioxidant) through enzymolysis of a pectin shell layer, and the chitosan matrix effectively inhibits the nonspecific burst release of active substances. The invention realizes the accurate targeted release of active ingredients, can efficiently inhibit the growth and oxidative deterioration of microorganisms, is suitable for the fresh-keeping package of high-moisture protein foods such as refrigerated fish and the like, and provides a novel multifunctional solution for intelligent package.

Inventors

  • CHEN RUI
  • LI SHUHUAI

Assignees

  • 中国热带农业科学院分析测试中心

Dates

Publication Date
20260505
Application Date
20260305

Claims (7)

  1. 1. The preparation method of the intelligent pectase response type chitosan nano composite packaging film is characterized by comprising the following steps of: Step one, preparation of ternary nanocomposite (1.1) Dissolving pectin in deionized water to obtain pectin solution; (1.2) dissolving nisin in 0.75% by volume of aqueous acetic acid to obtain nisin solution; (1.3) dissolving anthocyanin in 70 volume percent ethanol water solution to obtain anthocyanin solution; (1.4) dropwise adding the anthocyanin solution into the pectin solution under the continuous stirring of 300-600 rpm, continuously stirring for 30min after the dropwise adding is finished to form anthocyanin-pectin nano-composite suspension, then adding the nisin solution into the anthocyanin-pectin nano-composite suspension, and continuously stirring for 1h to obtain anthocyanin-pectin-nisin ternary nano-composite suspension; step two, preparation of composite packaging film (2.1) Dissolving 2% of chitosan aqueous solution in volume percent into 2% of acetic acid aqueous solution, adding glycerol with the weight of 20% of chitosan as a plasticizer, and continuously stirring at 55 ℃ for 30min until the chitosan aqueous solution is completely dissolved to obtain a chitosan solution; (2.2) mixing the APN NPs suspension prepared in the step one with chitosan solution according to the volume ratio of 1:10, and performing ultrasonic treatment on the mixed solution for 30min to remove bubbles to obtain film forming solution; and (2.3) casting the film forming liquid into a mould, drying at 40 ℃, peeling, and balancing for 48 hours at 25 ℃ and 50% relative humidity to obtain the composite packaging film.
  2. 2. The method for preparing the intelligent pectase response type chitosan nanocomposite packaging film according to claim 1, wherein the concentration of the pectic solution in the step (1.1) is 1mg/mL.
  3. 3. The method for preparing the intelligent pectase response type chitosan nanocomposite packaging film according to claim 1, wherein the concentration of the nisin solution in the step (1.2) is 3mg/mL.
  4. 4. The method for preparing the intelligent pectase response type chitosan nanocomposite packaging film according to claim 1, wherein the concentration of the anthocyanin solution in the step (1.3) is 1mg/mL.
  5. 5. The method for preparing the intelligent pectase response type chitosan nanocomposite packaging film according to claim 1, wherein in the step (1.4), the volume ratio of anthocyanin solution to pectin solution to nisin solution=1:10:1.
  6. 6. An intelligent pectinase response type chitosan nanocomposite packaging film prepared by the method of any one of claims 1 to 5.
  7. 7. An application of the intelligent pectase response type chitosan nanocomposite packaging film prepared by the method of any one of claims 1-5 or the intelligent pectase response type chitosan nanocomposite packaging film of claim 6 in fresh-keeping packaging of refrigerated fish.

Description

Intelligent pectase response type chitosan nano composite packaging film and preparation method and application thereof Technical Field The invention relates to the technical field of preservative film materials, in particular to an intelligent pectinase response type chitosan nano composite packaging film, and a preparation method and application thereof. Background Food packaging is critical to extending shelf life, ensuring safety, and reducing waste. While active and smart packaging systems are of interest for integrating functional substances and enabling real-time quality monitoring, many traditional materials still face the problem of uncontrolled "burst" of active compounds during storage, leading to premature exhaustion of functionality and inefficient use of resources. The ideal system should have a "trigger release" mechanism, i.e. the active substance is released only in response to a specific spoilage signal, thereby optimizing efficacy and minimizing waste. A variety of stimulus response mechanisms have been studied for packaging applications such as pH, temperature, and enzyme triggering. Among them, enzyme triggered systems have unique advantages due to their high specificity and biological relevance to food spoilage processes. Notably, spoilage microorganisms (e.g., pseudomonas) in refrigerated fish secrete pectase enzymes during growth, which provide a natural and targeted biological "switch" for the intelligent delivery system. The invention adopts pectin as enzyme responsive shell material. Upon contact with pectinase, the shell is hydrolyzed, triggering the on-demand release of the encapsulated active. To impart functionality to the system, anthocyanidin is introduced as a natural antioxidant and nisin is utilized as both an antimicrobial agent and a structural component of the nanoparticle wall. Chitosan is a linear cationic polysaccharide which is widely used in the field of food packaging due to its film forming property, antibacterial activity and biocompatibility. In order to enhance the mechanical property and the barrier property, chitosan is often used in combination with other polysaccharides such as pectin. In the present invention, chitosan serves as a secondary physical barrier in a composite membrane in which anthocyanin-loaded pectin-nisin nanoparticles are embedded. The design prolongs the diffusion path of the packaging material, effectively inhibits nonspecific burst release, ensures that active ingredients (anthocyanin and nisin) are selectively and efficiently released only under the triggering of pectase in the target spoilage microenvironment, and realizes accurate on-demand fresh-keeping. Disclosure of Invention In view of this, the present invention provides a novel pectinase responsive chitosan-based nanocomposite packaging system. In order to achieve the above purpose, the invention adopts the following technical scheme: firstly, the invention provides a preparation method of an intelligent pectinase response type chitosan nano composite packaging film, which comprises the following steps: Step one, preparation of ternary nanocomposite (1.1) Dissolving pectin in deionized water to obtain pectin solution; (1.2) dissolving nisin in 0.75% by volume of aqueous acetic acid to obtain nisin solution; (1.3) dissolving anthocyanin in 70 volume percent ethanol water solution to obtain anthocyanin solution; (1.4) dropwise adding the anthocyanin solution into the pectin solution under the continuous stirring of 300-600 rpm, continuously stirring for 30min after the dropwise adding is finished to form anthocyanin-pectin nanocomposite suspension, then adding the nisin solution into the anthocyanin-pectin nanocomposite suspension, and continuously stirring for 1h to obtain anthocyanin-pectin-nisin ternary nanocomposite suspension (marked as APN NPs); step two, preparation of composite packaging film (2.1) Dissolving 2% of chitosan aqueous solution in volume percent into 2% of acetic acid aqueous solution, adding glycerol with the weight of 20% of chitosan as a plasticizer, and continuously stirring at 55 ℃ for 30min until the chitosan aqueous solution is completely dissolved to obtain a chitosan solution; (2.2) mixing the APN NPs suspension prepared in the step one with chitosan solution according to the volume ratio of 1:10, and performing ultrasonic treatment on the mixed solution for 30min to remove bubbles to obtain film forming solution; and (2.3) casting the film forming liquid into a mould, drying at 40 ℃, peeling, and balancing for 48 hours at 25 ℃ and 50% relative humidity to obtain the composite packaging film. Preferably, the pectin solution in step (1.1) has a concentration of 1mg/mL. Preferably, the nisin solution in step (1.2) is at a concentration of 3mg/mL. Preferably, the anthocyanin solution in step (1.3) has a concentration of 1mg/mL. Preferably, in step (1.4), the volume ratio of anthocyanin solution to pectin solution to nisin solution=1:10:1. The inventio