CN-121975518-A - Photo-thermal carbon quantum dot based on rhein, and preparation method and application thereof

Abstract

The invention relates to the technical field of material science, in particular to a photo-thermal carbon quantum dot based on rhein, a preparation method and application thereof. The preparation method comprises the steps of dissolving rhein and small molecular peptide in a solvent to obtain a mixed solution, and carrying out hydrothermal reaction on the mixed solution to obtain the photo-thermal carbon quantum dot. The invention uses a one-step hydrothermal method as a core process, and realizes the comprehensive advantages of simple process, low energy consumption, good consistency among batches and easy industrialization while maintaining high photo-thermal performance; the photo-thermal carbon quantum dot based on rhein has strong and wide light absorption capacity, gao Guangre conversion efficiency, good biocompatibility and low toxicity.

Inventors

• HUANG QIAOXIAN
• Chen Lianjuan
• LAI YUYUAN
• YANG YUTONG
• LIU YINJING
• ZHANG YANJUN

Assignees

• 北部湾大学

Dates

Publication Date

20260505

Application Date

20260129

Claims (10)

1. 1. The preparation method of the photo-thermal carbon quantum dot based on rhein is characterized by comprising the following steps of: Dissolving rhein and small molecular peptide in solvent to obtain mixed solution, and performing hydrothermal reaction to obtain the photo-thermal carbon quantum dot.
2. 2. The method for preparing the photo-thermal carbon quantum dot based on rhein as claimed in claim 1, wherein the small molecule peptide comprises glutathione; The solvent includes ultrapure water.
3. 3. The method for preparing the photo-thermal carbon quantum dot based on rhein according to claim 1, wherein the mass ratio of rhein to the small molecule peptide is 1:1.081; The mass ratio of rhein to the solvent is 142:15-25.
4. 4. The method for preparing the photo-thermal carbon quantum dot based on rhein according to any one of claims 1 to 3, wherein the step of performing a hydrothermal reaction on the mixed solution to obtain the photo-thermal carbon quantum dot comprises the specific steps of performing the hydrothermal reaction on the mixed solution to obtain a reaction solution, and sequentially performing rotary evaporation, dialysis and drying on the reaction solution to obtain the photo-thermal carbon quantum dot.
5. 5. The method for preparing the photo-thermal carbon quantum dot based on rhein according to claim 4, wherein the hydrothermal reaction is carried out at a temperature of 160-220 ℃, and the autogenous vapor pressure is 0.3-4 MPa for 4-6 h.
6. 6. The method for preparing the photo-thermal carbon quantum dot based on rhein as claimed in claim 4, wherein, The molecular weight cut-off of the dialysis bag for dialysis is 500Da, and the dialysis time is 24-48 hours.
7. 7. A photo-thermal carbon quantum dot based on rhein, which is characterized by being prepared by the preparation method of any one of claims 1 to 6.
8. 8. The rhein-based photo-thermal carbon quantum dot of claim 7, wherein the average particle size of the rhein-based photo-thermal carbon quantum dot is 2-6 nm.
9. 9. The application of the rhein-based photo-thermal carbon quantum dot is characterized in that the rhein-based photo-thermal carbon quantum dot is used for preparing a photo-thermal treatment or photo-thermal imaging product according to claim 7.
10. 10. The use of a photo-thermal carbon quantum dot based on rhein as claimed in claim 9, wherein the product comprises any one of a medicine and a kit.

Description

Photo-thermal carbon quantum dot based on rhein, and preparation method and application thereof Technical Field The invention relates to the technical field of material science, in particular to a photo-thermal carbon quantum dot based on rhein, a preparation method and application thereof. Background Carbon quantum dots are another emerging class of zero-dimensional carbon nano-optical materials with dimensions less than 10 nm, following fullerenes, carbon nanotubes, and graphene. The material consists of an sp2 hybridized conjugated carbon core and a functional group-rich surface, has excellent water solubility, low toxicity, renal clearance, easy surface modification, adjustable fluorescence/near infrared absorption and high-efficiency photothermal conversion capability, and is regarded as a next-generation photothermal candidate material for photothermal treatment. Photothermal therapy is a minimally invasive therapeutic means for converting light energy into local hyperthermia (42 ℃ to 48 ℃) by exciting a photothermal conversion agent by using near infrared light (NIR, wavelength of 700 nm to 1100 nm) so as to selectively kill tumor cells. Compared with the traditional radiotherapy and chemotherapy, the method has the advantages of strong space-time controllability, low systemic toxicity, capability of activating immune response and the like, and has become a research hot spot for auxiliary treatment of solid tumors in recent years. However, the prior art still has the defects of low photo-thermal efficiency, poor tumor targeting, insufficient photo-thermal stability and the like of most carbon quantum dots. Rhein is anthraquinone star molecule in radix et rhizoma Rhei, widely existing in radix et rhizoma Rhei and Polygoni Multiflori radix, and has antiinflammatory, antioxidant, antibacterial and potential antitumor effects. Its advantages are high effect on scavenging free radicals, inhibiting growth of pathogenic bacteria, and regulating lipid metabolism. In addition, compared with other anthraquinone components, the anthraquinone compound has lower irritation and better short-term use safety. However, rhein also has the limitations of poor water solubility, low bioavailability and the like, and affects the efficacy. If the application of rhein in clinical effect is to be further improved, the dosage form or structure of rhein still needs to be optimized to improve the safety and reduce the side effect. The preparation of the carbon quantum dots by taking rhein as a carbon source is an effective method for solving the rhein defect, realizes the enlargement of conjugated domain, and shows excellent photo-thermal performance in deep red-near infrared band. Compared with the visible light wave band, near infrared light (700 nm-1700 nm) has deeper tissue penetration depth, less scattering and lower tissue autofluorescence, and is an ideal wave band for applications such as biological imaging, photo-thermal treatment, photodynamic therapy and the like. Therefore, the preparation scheme which reasonably and effectively improves the poor water solubility and low bioavailability of the rheum officinale water and the preparation of the carbon quantum dots with the photo-thermal effect are urgent. In view of the above, the invention provides a photo-thermal carbon quantum dot based on rhein, a preparation method and application thereof. Disclosure of Invention The invention aims to provide a photo-thermal carbon quantum dot based on rhein, a preparation method and application thereof. The invention aims to provide a rhein-based photo-thermal carbon quantum dot composite nanomaterial, which solves the defects of low photo-thermal conversion efficiency and insufficient tumor enrichment in the prior art, and the carbon quantum dot has high NIR-II region photo-thermal conversion efficiency and good photo-thermal stability. The technical scheme for solving the technical problems is as follows: in a first aspect, a method for preparing photo-thermal carbon quantum dots based on rhein comprises the following steps: Dissolving rhein and small molecular peptide in solvent to obtain mixed solution, and performing hydrothermal reaction to obtain the photo-thermal carbon quantum dot. On the basis of the technical scheme, the invention can be improved as follows. Further, the small molecule peptide comprises glutathione, wherein the glutathione is formed by connecting glutamic acid, cysteine and glycine through peptide bonds; The solvent includes ultrapure water. Further, the mass ratio of rhein to the small molecule peptide is 1:1.081; The mass ratio of rhein to the solvent is 142:15-25. Further, the mixed solution is subjected to hydrothermal reaction to obtain the photo-thermal carbon quantum dot, and the photo-thermal carbon quantum dot is obtained by carrying out hydrothermal reaction on the mixed solution to obtain a reaction solution, and sequentially carrying out rotary evaporation, dialysis and drying on the react