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CN-121975527-A - Soil restoration agent for petroleum-type polluted soil and preparation method thereof

CN121975527ACN 121975527 ACN121975527 ACN 121975527ACN-121975527-A

Abstract

The invention relates to the technical field of soil remediation, in particular to a soil remediation agent for petroleum-type polluted soil and a preparation method thereof, which are used for solving the problem that the existing petroleum-type polluted soil is difficult to naturally remediate; the preparation method has the advantages that the contaminated soil is repaired by using a bioremediation method, the petroleum hydrocarbon pollutants are removed by adding the flora into the contaminated soil, the efficiency is high, the effect is good, the environmental destruction is small, the candida tropicalis and the streptococcus faecalis are adopted for composite treatment, the candida tropicalis has the advantages of high permeation resistance, acid resistance and high metabolic efficiency of fungi, and can generate pseudomycelium and spores, an extended network structure can be formed in the soil, the network structure can be used as a bacterial migration channel, the bacterial migration is convenient, the treatment effect on the pollutants is improved, the streptococcus faecalis can grow in the environment where the petroleum hydrocarbon exists, the degradation effect on the petroleum hydrocarbon is good, and the good biological degradation effect can be achieved by compounding the candida tropicalis and the streptococcus faecalis.

Inventors

  • ZHANG YOUCHAO
  • ZHU HONGXIA
  • ZHANG JINGYUE

Assignees

  • 江西润垚生物科技有限公司

Dates

Publication Date
20260505
Application Date
20260109

Claims (8)

  1. 1. The preparation method of the soil restoration agent for the petroleum-type polluted soil is characterized by comprising the following steps of: weighing 40-80 parts of carrier bacteria, 8-32 parts of dispersing agent, 25-35 parts of humic acid, 5-15 parts of urea and 8-16 parts of fly ash according to parts by weight for standby; Uniformly mixing carrier bacteria, dispersing agent, humic acid, urea and fly ash to obtain a soil remediation agent for petroleum contaminated soil; Wherein, the carrier bacteria are prepared by the following steps: s1, crushing, cleaning and drying peanut shells, and then placing the peanut shells in a tube furnace for thermal pyrolysis to obtain biochar; s2, adding the biochar into a magnesium chloride solution for soaking, drying, washing, and then drying again to obtain modified biochar; s3, respectively inoculating candida tropicalis and streptococcus faecalis into an expansion culture medium for constant-temperature shaking culture to respectively obtain candida tropicalis bacteria liquid and streptococcus faecalis bacteria liquid; S4, mixing candida tropicalis bacteria liquid and streptococcus faecalis bacteria liquid to form mixed bacteria liquid, adding the modified biochar into the mixed bacteria liquid, and uniformly stirring to form carrier bacteria.
  2. 2. The method for preparing a soil restoration agent for petroleum-type contaminated soil according to claim 1, wherein the usage amount of the biochar and magnesium chloride solution in the step S2 is 1g:20-30mL, and the molar concentration of the magnesium chloride solution is 1-1.5mol/L.
  3. 3. The method for preparing the soil remediation agent for petroleum-type contaminated soil according to claim 1, wherein the candida tropicalis bacteria liquid, streptococcus faecalis bacteria liquid and modified biochar in the step S4 are used in a ratio of 10g to 25-35g.
  4. 4. The method for preparing a soil restoration agent for petroleum-based contaminated soil according to claim 1, wherein the diffusion agent is prepared by the steps of: a1, adding phenyl dichlorophosphate, melamine, ethylene glycol and triethylamine into a three-neck flask, stirring for reaction, cooling a reaction product to room temperature after the reaction is finished, adding the reaction product into absolute ethyl alcohol, precipitating a precipitate, performing vacuum suction filtration, and drying a filter cake to obtain an intermediate 1; a2, adding the intermediate 1, 4-bromoethyl butyrate, potassium carbonate and toluene into a three-neck flask, stirring for reaction, cooling a reaction product to room temperature after the reaction is finished, vacuum filtering, and rotationally evaporating filtrate to obtain an intermediate 2; A3, adding the intermediate 2, potassium hydroxide, deionized water and tetrahydrofuran into a three-neck flask, stirring for reaction, then dropwise adding hydrochloric acid solution while stirring, continuing stirring for reaction after the dropwise adding is finished, vacuum filtering a reaction product after the reaction is finished, and washing, drying and recrystallizing a filter cake to obtain an intermediate 3; A4, adding the intermediate 3 and thionyl chloride into a three-neck flask, stirring for reaction, cooling a reaction product to room temperature after the reaction is finished, and then performing rotary evaporation to obtain an intermediate 4; And A5, adding the intermediate 4, glycine, potassium hydroxide solution and tetrahydrofuran into a three-neck flask, stirring for reaction, then adding hydrochloric acid solution dropwise while stirring, continuing stirring for reaction after the dripping is finished, and removing a solvent by rotary evaporation of a reaction product after the reaction is finished to obtain the dispersing agent.
  5. 5. The method for preparing a soil restoration agent for petroleum-type contaminated soil according to claim 4, wherein the dosage ratio of phenyl dichlorophosphate, melamine, ethylene glycol and triethylamine in the step A1 is 0.1mol:0.2mol:60-80mL:1.5-2.5mL, and the dosage ratio of intermediate 1, ethyl 4-bromobutyrate, potassium carbonate and toluene in the step A2 is 10mmol:45-50mmol:40-50mmol:80-100mL.
  6. 6. The preparation method of the soil remediation agent for petroleum-type contaminated soil according to claim 4, wherein the dosage ratio of the intermediate 2 to the potassium hydroxide to the deionized water to the tetrahydrofuran in the step A3 is 10mmol:2-3g:20-25mL:40-50mL, the mass fraction of the hydrochloric acid solution is 20-25%, and the dosage ratio of the intermediate 3 to the thionyl chloride in the step A4 is 1g:20-25mL.
  7. 7. The method for preparing a soil remediation agent for petroleum-type contaminated soil according to claim 4, wherein the intermediate 4, the glycine, the potassium hydroxide solution and the tetrahydrofuran in the step A5 are used in a ratio of 10mmol:45-50mmol:50-60ml:50-60mL, the mass fraction of the potassium hydroxide solution is 10-12%, and the mass fraction of the hydrochloric acid solution is 20-25%.
  8. 8. A soil restoration agent for petroleum-based contaminated soil, characterized in that the soil restoration agent for petroleum-based contaminated soil is prepared by the method for preparing the soil restoration agent for petroleum-based contaminated soil according to any one of claims 1 to 7.

Description

Soil restoration agent for petroleum-type polluted soil and preparation method thereof Technical Field The invention relates to the technical field of soil remediation, in particular to a soil remediation agent for petroleum-type polluted soil and a preparation method thereof. Background Petroleum is very important in the current industrial economy, and the modern industry and economic development have great demands for petroleum, and the production amount thereof is always high. In the course of petroleum exploitation, petroleum transportation and petroleum processing, environmental pollution accidents including atmospheric, water and soil pollution and the like are frequent. Local severe pollution can have serious impact on the ecological environment and also pose a threat to human health, and such pollution usually takes a long time to recover. Petroleum hydrocarbon is the most used fossil fuel in the world at present, there is petroleum leakage in petroleum exploitation and petroleum transportation to lead to environmental pollution's risk, the excessive of petroleum, storage etc. of ageing fatlute all can cause serious environmental pollution, once take place to leak, will cause serious environmental pollution, at first its leakage can lead to serious soil environmental pollution, because soil particle's porous nature and adsorptivity, petroleum hydrocarbon has higher viscidity and extremely strong hydrophobicity, it is adsorbed by soil particle easily, make soil particle's water permeability and gas permeability drop sharply, the destruction of destructive to ecology has caused nearly to vanish, it is almost impossible to reliably naturally restore in this kind of high concentration petroleum hydrocarbon polluted environment. How to improve the existing petroleum-based contaminated soil is difficult to repair naturally is the key of the invention, so that a soil repair agent for petroleum-based contaminated soil and a preparation method thereof are needed to solve the above problems. Disclosure of Invention In order to overcome the technical problems, the invention aims to provide the soil restoration agent for the petroleum-type polluted soil and the preparation method thereof, wherein carrier bacteria, a dispersing agent, humic acid, urea and fly ash are uniformly mixed to obtain the soil restoration agent for the petroleum-type polluted soil, and the soil restoration agent for the petroleum-type polluted soil is added into the petroleum-type polluted soil, so that the problem that the existing petroleum-type polluted soil is difficult to naturally restore is solved. The aim of the invention can be achieved by the following technical scheme: The preparation method of the soil restoration agent for petroleum-type polluted soil comprises the following steps: weighing 40-80 parts of carrier bacteria, 8-32 parts of dispersing agent, 25-35 parts of humic acid, 5-15 parts of urea and 8-16 parts of fly ash according to parts by weight for standby; Uniformly mixing carrier bacteria, dispersing agent, humic acid, urea and fly ash to obtain a soil remediation agent for petroleum contaminated soil; Wherein, the carrier bacteria are prepared by the following steps: s1, crushing, cleaning and drying peanut shells, then placing the peanut shells in a tube furnace, and thermally cracking the peanut shells for 3-5 hours under the conditions that the temperature is 400-420 ℃ and the nitrogen flow is 50m 3/h to obtain biochar; S2, adding biochar into a magnesium chloride solution, soaking for 2-3 hours at the temperature of 25-30 ℃, then placing in a vacuum drying oven, drying for 1-2 hours at the temperature of 100-110 ℃, then washing for 3-5 times by using distilled water, and then drying again to obtain modified biochar; S3, respectively inoculating candida tropicalis and streptococcus faecalis into an expansion culture medium according to the proportion of 10%, and then carrying out constant-temperature shaking culture for 24 hours at the temperature of 25-30 ℃ until the average value is more than 1X 10 9 cfu/mL by adopting a microscopic direct counting method to respectively obtain candida tropicalis liquid and streptococcus faecalis liquid; S4, mixing candida tropicalis bacteria liquid and streptococcus faecalis bacteria liquid to form mixed bacteria liquid, adding the modified biochar into the mixed bacteria liquid, and uniformly stirring to form carrier bacteria. As a further scheme of the invention, the dosage ratio of the biochar to the magnesium chloride solution in the step S2 is 1g:20-30mL, and the molar concentration of the magnesium chloride solution is 1-1.5mol/L. As a further scheme of the invention, the candida tropicalis bacteria liquid, streptococcus faecalis bacteria liquid and modified biochar in the step S4 are used in the dosage ratio of 10g to 25-35g. As a further scheme of the invention, the dispersing agent is prepared by the following steps: A1, adding phenyl dichlorophosphate, melamine, e