CN-121975601-A - Full-automatic sequencing library-building instrument and control method thereof

CN121975601ACN 121975601 ACN121975601 ACN 121975601ACN-121975601-A

Abstract

The application discloses a full-automatic sequencing library-building instrument and a control method thereof. The warehouse-building instrument comprises a cover opening component, wherein the cover opening component is synchronously abutted against a downward turning part and an upward turning part of the flip through a first abutting part, so that the flip enters a half-open or slightly-open state to balance pressure difference, and the flip is completely opened by pressing the downward turning part under a second abutting part. When the cover is closed, the abutting piece is physically guided by utilizing the groove-shaped structure on the surface of the flip cover, so that accurate buckling is ensured. The control method is used for nesting the biochemical process and the step switch cover logic deeply. The application effectively solves the problems of liquid splashing and pollution in the automatic cover opening process, improves the cover closing reliability and experimental safety, and realizes the standardized automatic operation of the whole process from nucleic acid extraction to library construction.

Inventors

LI WENXIANG
HAO MAOLEI
WU ZAIHUI

Assignees

郑州中科生物医学工程技术研究院

Dates

Publication Date: 20260505
Application Date: 20251226

Claims (10)

1. A full-automatic sequencing library-building instrument, comprising: The device comprises a rack, a pipetting module and an incubation transmission module, wherein the pipetting module and the incubation transmission module are arranged in the rack; the incubation transmission module is used for bearing a kit; The pipetting module comprises a multi-axis movement mechanism, a pipetting module and a cover opening assembly; The multi-axis movement mechanism is connected with the pipetting module and the uncovering component and is used for driving the pipetting module and the uncovering component to move relative to the incubation transmission module, The cover opening assembly comprises an abutting piece for performing downward-pressing cover opening; When the abutting part presses down the cover, the abutting part abuts against the flip cover of the kit, so that the flip cover is turned to a half-open state or a micro-open state firstly and then moves to a full-open state.
2. The fully automatic sequencing library creator of claim 1, wherein, The cover opening assembly further comprises a fixing seat, wherein the abutting piece extends along the direction of downwards pressing the cover opening of the fixing seat, and comprises a first abutting part and a second abutting part; the first abutting part is used for pressing down the upturning part and the downturning part of the flip cover in a closed state, so that the flip cover is opened to a half-opened or slightly opened state; The second abutting part is used for pressing down the downward turning part of the flip cover in a half-open or slightly-open state, so that the flip cover is opened to a full-open state.
3. The fully automatic sequencing library generator of claim 2, wherein the first abutment comprises a first rod and a second rod, the second abutment comprises a third rod; The first rod body is used for pressing the downward turning part to trigger opening and is matched with the upward turning part which is propped up by the second rod body so as to jointly maintain the semi-opening or micro-opening state; And the third rod body is used for continuously pressing the downward turning part after the half-opening or slightly-opening state so as to drive the flip cover to the full-opening state.
4. The fully automatic sequencing library builder of claim 2, wherein the first and second abutments are different contact surfaces of the abutment bottom end; The first abutting part can contact the downward turning part and the upward turning part when the downward pressing stroke is carried out on the flip cover, so that the flip cover is turned to a half-open or slightly-open state; The second abutting part can contact the downward turning part when the downward pressing stroke is carried out on the turning cover, so that the turning cover is opened to a complete opening state.
5. The fully automatic sequencing library creator of claim 1, wherein, The multi-axis movement mechanism is used for switching the opening state of the flip cover by driving the third abutting part and the fourth abutting part to extend out of different lengths; the third abutting part stretches out of a first elongation to abut against the downward turning part of the flip cover, and the fourth abutting part stretches out of a second elongation to abut against the upward turning part, so that the flip cover is turned to a half-open state or a slight-open state firstly; and the third extension amount is extended through the third abutting part to abut against the downward turning part of the flip cover, and the fourth abutting part is separated from the upward turning part, so that the flip cover is opened to a full opening state, wherein the third extension amount, the first extension amount and the second extension amount are sequentially reduced.
6. A control method of a fully automatic sequencing and library-building instrument, which is characterized by being applied to the fully automatic sequencing and library-building instrument according to claim 1, wherein the fully automatic sequencing and library-building instrument comprises the following steps in the process of performing nucleic acid extraction or library construction: the cover opening assembly is controlled to execute a first downward pressing action, and the cover opening assembly is synchronously abutted against the downward turning part and the upward turning part of the reagent box cover so as to enable the cover to be pressed down and opened to be in a half-opened or slightly opened state; performing a target action of the current nucleic acid extraction or library construction; And controlling the cover opening assembly to close the cover opening.
7. The control method of claim 6, wherein the door assembly comprises a first lever, a second lever, and a third lever, the third lever having a length greater than a length of the first lever, the first lever having a length greater than a length of the second lever; the control the cover opening assembly executes a first downward pressing action, and the cover opening assembly synchronously abuts against the downward turning part and the upward turning part of the reagent box cover, so that the cover is pressed down and opened to be in a half-opened or slightly opened state, and the method specifically comprises the following steps: Controlling the multi-axis movement mechanism to drive the cover opening assembly to move to a first cover opening position so as to align the first rod body with the downward turning part of the reagent box flip cover, and aligning the second rod body with the upward turning part of the reagent box flip cover; The cover opening assembly is controlled to execute a first downward pressing action, so that the first rod body is abutted against the downward turning part of the reagent box cover, and the second rod body is abutted against the upward turning part of the reagent box cover, so that the cover is pressed down to be opened to the half-opened or slightly opened state; The step of controlling the cover opening assembly to execute a second pressing action so that the cover is pressed from the half-opened state or the micro-opened state to the full-opened state specifically comprises the following steps: Controlling the multi-axis movement mechanism to drive the cover opening assembly to move to a second cover opening position so as to align the third rod body to the turndown part of the reagent box turnover cover; And controlling the cover opening assembly to execute a second pressing action, so that the third rod body is abutted against the downward turning part of the reagent box cover, and the cover is pressed and opened to the completely opened state.
8. The control method of claim 7, wherein said controlling said lid opening assembly to close said lid comprises the steps of: Controlling the cover opening assembly to perform lifting action at a second cover opening position so as to enable the bottom end position of the third rod body to be aligned with the top surface of the flip cover in a closed state; And controlling the multi-axis movement mechanism to drive the third rod body to move to a cover closing position positioned on the surface of the upturned part so as to close the flip cover.
9. The control method according to claim 6, wherein the abutting piece includes a third abutting portion and a fourth abutting portion that are independently adjustable in telescoping length; The step of controlling the cover opening assembly to execute a first downward pressing action, and synchronously abutting the downward turning part and the upward turning part of the reagent box cover opening through the cover opening assembly, so that the cover opening is pressed down and opened to a half-opened or slightly opened state, and the method further comprises the steps of: Controlling the cover opening assembly to move to align with the flip cover so that the third abutting portion aligns with the downward turning portion of the reagent box flip cover and the fourth abutting portion aligns with the upward turning portion of the reagent box flip cover; the control the cover opening assembly executes a first downward pressing action, and the cover opening assembly synchronously abuts against the downward turning part and the upward turning part of the reagent box cover, so that the cover is pressed down and opened to be in a half-opened or slightly opened state, and the method specifically comprises the following steps: Controlling the third abutting part to extend out of a first elongation to abut against the downward turning part, and synchronously controlling the fourth abutting part to extend out of a second elongation to abut against the upward turning part, so that the flip cover is pressed down to be opened to the half-opened or slightly opened state, wherein the first elongation is larger than the second elongation; The step of controlling the cover opening assembly to execute a second pressing action so that the cover is pressed from the half-opened state or the micro-opened state to the full-opened state comprises the following steps: and controlling the third abutting part to extend from the first extension amount to the third extension amount, and controlling the fourth abutting part to be separated from the upturning part so as to enable the flip to be pressed down and opened to the fully opened state.
10. The control method of claim 9, wherein said controlling said lid opening assembly to close said lid comprises the steps of: Controlling the cover opening assembly to perform lifting action, and switching the third abutting part to the third extension amount so as to enable the bottom end height of the third abutting part to be aligned with the top surface height of the flip cover in a closed state; and controlling the multi-axis movement mechanism to drive the third abutting part to move to a cover closing position on the surface of the upturned part so as to close the flip cover.

Description

Full-automatic sequencing library-building instrument and control method thereof Technical Field The application relates to the technical field of biomedical equipment and laboratory automation, in particular to a full-automatic sequencing library-building instrument and a control method thereof. Background In the field of gene sequencing, library construction is a key element in determining sequencing quality. The existing nucleic acid extraction and library construction processes depend on manual or semi-automatic equipment, and have the defects of complex operation, easiness in sample cross contamination, aerosol pollution and the like. With the popularity of high throughput sequencing, the development of highly automated library-building equipment has become an industry trend. However, existing automated equipment still presents significant technical bottlenecks in handling capped kits. On the one hand, after the kit is subjected to heating incubation or biochemical reaction, the internal air pressure is higher than the external environment, and the traditional direct opening mode is extremely easy to cause liquid splash, so that the environmental pollution is caused and the experimental safety is threatened. On the other hand, the existing automatic cover closing mechanism has limited precision, and is often buckled untight or position offset in high-frequency operation, so that the reagent volatilizes and concentration is misaligned. Therefore, how to design a fully automatic sequencing and library-building scheme which can not only effectively prevent splashing but also ensure accurate cover closing is a problem to be solved by the technicians in the field. Disclosure of Invention In view of the shortcomings of the prior art, according to one aspect of the present application, there is provided a fully automated sequencing library builder comprising: The device comprises a rack, a pipetting module and an incubation transmission module, wherein the pipetting module and the incubation transmission module are arranged in the rack; The incubation transmission module is used for bearing the kit; the pipetting module comprises a multi-axis movement mechanism, a pipetting module and a cover opening assembly; the multi-axis movement mechanism is connected with the pipetting module and the uncapping assembly and is used for driving the pipetting module and the uncapping assembly to move relative to the incubation transmission module, The cover opening assembly comprises an abutting piece for performing downward cover opening; When the abutting part executes the downward pressure cover opening, the abutting part abuts against the flip cover of the kit, so that the flip cover is firstly turned to a half-open or slightly-open state and then moves to a full-open state. In an alternative embodiment, the cover opening assembly further comprises a fixed seat, an abutting piece, a first abutting part and a second abutting part, wherein the abutting piece extends along the downward cover opening direction of the fixed seat; the first abutting part is used for pressing down the upturning part and the downturning part of the flip cover in a closed state to enable the flip cover to be opened to a half-opened or slightly opened state; The second abutting part is used for pressing down the downward turning part of the flip cover in a half-open or slightly-open state, so that the flip cover is opened to a full-open state. In an alternative embodiment, the first abutting portion comprises a first rod body and a second rod body, and the second abutting portion comprises a third rod body; the first rod body is used for pressing down the turndown part to trigger the opening and is matched with the second rod body to prop against the sprung turnup part so as to jointly maintain a semi-opening or micro-opening state; The third rod body is used for continuously downwards pressing the turnover part after the half-opening or slightly-opening state so as to drive the turnover cover to be in a complete opening state. In an alternative embodiment, the first abutment and the second abutment are different contact surfaces of the abutment bottom end; the first abutting part can contact the downward turning part and the upward turning part when the downward pressing stroke is carried out on the flip cover, so that the flip cover is turned to a half-open or slightly-open state; The second abutting part can contact the downward turning part when the turnover cover executes the downward stroke, so that the turnover cover is opened to a complete opening state. In an alternative embodiment, the abutting piece comprises a third abutting part and a fourth abutting part which can independently adjust the telescopic length, wherein the multiaxial movement mechanism is used for switching the opening state of the flip cover by driving the third abutting part and the fourth abutting part to extend out of different lengths; the third abutting part stre