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CN-121975602-A - Nucleic acid detection consumable

CN121975602ACN 121975602 ACN121975602 ACN 121975602ACN-121975602-A

Abstract

The invention discloses a nucleic acid detection consumable, and belongs to the field of nucleic acid detection. The multifunctional sample splitting and mixing device comprises a swab pipe, a shell, a roller component, a pipeline and a PCR (polymerase chain reaction) pipe, wherein the shell is used for accommodating and fixing the swab pipe, the PCR pipe, the pipeline and the roller component, the bottom of the swab pipe is communicated with the top of the PCR pipe through the pipeline, freeze-drying reagents are preloaded in the PCR pipe, the roller component is tightly pressed on the outer side of the pipeline, the swab pipe is an integrated connection design of a nucleic acid detection consumable, the pipeline is a nucleic acid detection consumable and the PCR pipe, the swab pipe is a nucleic acid detection consumable integrates sample splitting and mixing functions, a dilution pipe is not required, the shell is matched with the nucleic acid detection consumable to integrate and fix all components, the integral structure is greatly simplified, the occupied space is reduced, and compared with a traditional split test tube, the multifunctional sample splitting and mixing device is more convenient to carry and transfer, and is suitable for various detection scene requirements.

Inventors

  • XIE YAPING

Assignees

  • 解亚平

Dates

Publication Date
20260505
Application Date
20260129

Claims (6)

  1. 1. A nucleic acid detection consumable comprises a swab tube (1), a shell (2), a roller assembly (3), a pipeline (4) and a PCR tube (5), and is characterized in that the shell (2) is used for accommodating and fixing the swab tube (1), the PCR tube (5), the pipeline (4) and the roller assembly (3), the bottom of the swab tube (1) is communicated with the top of the PCR tube (5) through the pipeline (4), a freeze-drying reagent is preloaded in the PCR tube (5), the roller assembly (3) is tightly pressed on the outer side of the pipeline (4), the roller assembly (3) can rotate relative to the pipeline (4), a valve structure is formed when the roller assembly (3) does not rotate, the swab tube (1) is separated from the PCR tube (5), and liquid in the swab tube (1) can be quantitatively pumped into the PCR tube (5) when the roller assembly (3) rotates, so that the liquid is mixed with the freeze-drying reagent in the PCR tube (5).
  2. 2. The nucleic acid detecting consumable according to claim 1, wherein the roller assembly (3) comprises a roller and a knob, the knob is arranged on the side surface of the roller, and the roller can be driven to rotate by rotating the knob, so that quantitative conveying of liquid in the pipeline (4) is realized.
  3. 3. The nucleic acid detecting consumable according to claim 1, wherein the pipe (4) is made of flexible material, and the compacting and rolling actions of the roller assembly (3) are adapted to ensure that the pipe (4) is reliably blocked without liquid leakage when the roller assembly (3) does not rotate.
  4. 4. The nucleic acid detecting consumable according to claim 1, wherein the swab tube (1) is used for containing a sample liquid and a lysis solution, and provides a space for mixing the sample with the lysis solution and lysing the sample, and a dilution tube is not required to be additionally arranged, so that the whole structure is simplified.
  5. 5. The nucleic acid detection consumable according to claim 1, comprising a swab tube (1) and a PCR tube (5) which are mutually communicated, wherein the swab tube (1) and the PCR tube (5) are integrally connected through a pipeline (4), a freeze-drying reagent is preloaded in the PCR tube (5), and the pipeline (4) is matched with the compression and driving of the roller assembly (3) so as to realize quantitative and non-contact conveying of liquid in the swab tube (1) into the PCR tube (5).
  6. 6. The nucleic acid detecting consumable according to claim 1, wherein the swab tube (1), the pipeline (4) and the PCR tube (5) are of an integrated molding structure or a sealing connection structure, so that pollution risk in the liquid transferring process is reduced, and the nucleic acid detecting consumable is compact in structure and convenient to carry.

Description

Nucleic acid detection consumable Technical Field The invention relates to the field of nucleic acid detection, in particular to a nucleic acid detection consumable. Background PCR (PolymeraseChainReaction ) technology is a molecular biological technique that amplifies specific DNA (deoxyribonucleicacid ) sequences in vitro. The PCR technology has the characteristics of strong specificity, high sensitivity, low purity requirement, simplicity, convenience and rapidness, and is widely applied to molecular biological detection and analysis. Conventional nucleic acid detection is performed in molecular diagnostic laboratories, and is performed using a closed cassette. When the molecular diagnosis laboratory detects, according to the national regulation requirement, the molecular diagnosis laboratory needs to carry out partition treatment, namely a reagent preparation area, a nucleic acid extraction area, an amplification area and a detection area, and related experimenters need to have PCR on-duty certificates, so that strict requirements are provided for experimental operation environments and personnel experimental quality requirements. Therefore, when the molecular diagnosis laboratory detects, although the flux requirement can be met, the nucleic acid extraction device, the amplification device, the detection device and other auxiliary devices are required to be arranged separately, the equipment is complex, the occupied space is occupied, the detection automation degree is not improved, the risk of aerosol pollution exists when the nucleic acid extracting solution is transferred, and the detection accuracy cannot be ensured; With the development of molecular detection techniques, nucleic acid detection techniques have been widely used. In the nucleic acid detection process, a cracking tube, a dilution tube and a reaction tube are needed, the cracking tube is used for containing a cracking liquid and providing a mixed space for the cracking liquid and a sample liquid carrying a sample, the dilution tube is used for diluting the sample cracking mixed liquid, and the reaction tube is used for containing a specific process of the nucleic acid detection of the reaction liquid: opening a tube cover of a cracking tube, adding sample liquid into the cracking tube containing the cracking liquid, mixing the sample liquid with the cracking liquid to form sample cracking mixed liquid, cracking the sample in the cracking tube, opening the tube cover of a dilution tube, quantitatively extruding the sample cracking mixed liquid from the cracking tube to the dilution tube, mixing the sample cracking mixed liquid with the dilution liquid in the dilution tube, opening the tube cover of a reaction tube, quantitatively extruding the diluted sample cracking mixed liquid from the dilution tube to the reaction tube, mixing the diluted sample cracking mixed liquid with the reaction liquid in the reaction tube, and performing sample amplification and fluorescence detection. Because schizolysis pipe, dilution pipe and reaction tube separate independent setting, need frequent operation schizolysis pipe, dilution pipe and reaction tube at nucleic acid detection's in-process, and need frequent opening tube cap and add the operation, so, lead to the experimental step numerous, the operation degree of difficulty is great, and uses very inconvenient. In addition, frequent operation schizolysis pipe and reaction tube to carry out frequent joining operation, still increase the contact chance of sample liquid and external environment easily, lead to the sample contaminated, detect the precision and reduce. Moreover, the separate cracking tube and reaction tube occupy large space and are inconvenient to carry. Disclosure of Invention The invention aims to provide an integrated nucleic acid detection device, which solves the problems of complex equipment, large occupied space and low automation degree in the detection of the existing molecular diagnosis laboratory, and the problems of aerosol pollution risk and difficult detection accuracy guarantee during the transfer of a nucleic acid extracting solution, simplifies the structure of detection equipment, reduces space occupation, improves the detection automation level, avoids aerosol pollution, and ensures detection accuracy, and also aims to provide an integrated nucleic acid detection tube assembly, which solves the problems of multiple operation steps, large difficulty and inconvenient use caused by independent arrangement of a cracking tube, a dilution tube and a reaction tube in the existing nucleic acid detection, and the problems of large contact opportunity, easy pollution, large occupied space and inconvenient carrying of a sample and the external environment, reduces the operation steps and tube cover opening and closing times in the detection process, reduces the operation difficulty, reduces the sample pollution risk, improves the detection accuracy, and has a compact structure