CN-121975604-A - Vertical microorganism detection device and method

CN121975604ACN 121975604 ACN121975604 ACN 121975604ACN-121975604-A

Abstract

The invention belongs to the technical field of microorganism detection, and particularly relates to a vertical microorganism detection device and method; the device comprises a longitudinal movement module, a transverse movement module, a negative pressure pumping module and a shell, wherein the longitudinal movement module comprises a first rigid hollow pipe column, a second rigid hollow pipe column, a solid column, a fixed plate, a first tension spring and a second screw motor, the transverse movement module comprises a first screw motor, a third screw motor, a chip jig, a heating detection module, a guide rail, a second sliding block, a fixed frame and a push plate, the negative pressure pumping module comprises a first electromagnetic valve, a second electromagnetic valve and a stepping peristaltic pump, the detection device is matched with a microfluidic chip structure, different liquids in a chip can be driven to flow to a target cavity by only one stepping peristaltic pump, the peristaltic pump does not walk liquid, only walks gas, pollution is prevented in the experimental process as much as possible, and the accuracy of experimental results is ensured.

Inventors

Request for anonymity

Assignees

至微生物智能科技(厦门)有限公司

Dates

Publication Date: 20260505
Application Date: 20260327

Claims (10)

1. The vertical microorganism detection device is characterized by comprising a longitudinal movement module, a transverse movement module, a negative pressure pumping module and a shell, wherein the longitudinal movement module is positioned above the transverse movement module, and the transverse movement module and the longitudinal movement module are positioned in the shell in an initial state, and the vertical microorganism detection device comprises the following components: The micro-fluidic chip is placed in a chip groove formed by the chip jig, the fixing frame is driven by a motor to move transversely and linearly so as to drive the chip jig to move outside the shell to be placed in the micro-fluidic chip and move into the shell to enter an experimental state, and the push plate is driven by the motor to move transversely and linearly relative to the fixing frame so as to realize the opening or closing of different solution storage cavities in the micro-fluidic chip; The longitudinal movement module comprises a first rigid hollow tubular column, a second rigid hollow tubular column, a solid column, a fixed plate and a first tension spring; the first rigid hollow pipe column, the second rigid hollow pipe column and the solid column are respectively provided with a first guide plate and a second guide plate which are used for guiding the hollow pipe column and the solid column to move in the vertical direction, the first rigid hollow pipe column, the second rigid hollow pipe column and the solid column are fixedly provided with clamps, one ends of the clamps and the first tension springs are fixedly connected, the other ends of the first tension springs are fixedly connected with the second guide plates, a fixing plate is arranged near the position of the clamps, and when the fixing plate is driven by a motor to move linearly downwards, the first tension springs are always in a stretching state and the fixing plate is in butt joint with the clamps, so that the first rigid hollow pipe column, the second rigid hollow pipe column and the solid column can provide enough compression force when contacting the top surface of the microfluidic chip, and the sealing between the end surfaces of the first rigid hollow pipe column, the second rigid hollow pipe column and the solid column in the microfluidic chip and the first negative pressure pumping channel, the second negative pressure pumping channel and the cracking liquid cavity corresponding to the microfluidic chip is realized; the negative pressure pumping module is used for orderly driving the liquid stored in the microfluidic chip to flow.
2. The vertical microorganism detection device according to claim 1, wherein the microfluidic chip comprises a first solution storage part, a first slide valve, a second solution storage part, a second slide valve and a reaction part which are sequentially arranged from top to bottom, wherein a lysis solution cavity, a cleaning solution cavity, an eluent cavity, a reaction solution cavity, a first negative pumping channel serving as a total pumping opening and a second negative pumping channel serving as a sample pumping opening are arranged on the first solution storage part, a waste liquid cavity, a premixing cavity, a third channel and a fourth channel corresponding to the second negative pumping channel are arranged in the second solution storage part, a reaction cavity is arranged on the reaction part, a plurality of flow channels and through holes are arranged on the first slide valve to realize selective communication with solutions in the solution cavities on the first solution storage part and the second solution storage part, and selective communication with the through holes of the gas channels, the push plate moves relatively transversely and linearly under the driving of a motor to push the push plate so as to push the slide valve between the first solution storage part, the second solution storage part, the slide valve of the reaction part or the reaction part to be opened or closed.
3. The vertical microorganism detection device according to claim 2, wherein the longitudinal movement module further comprises a double-station self-holding electromagnet, a shifting fork metal plate, a piercing member and a second tension spring, one end of the second tension spring is fixedly arranged on the mounting seat, the other end of the second tension spring is fixedly connected with the piercing member, the piercing member penetrates through the second guide plate, the tip end of the piercing member is aligned with the cleaning liquid cavity, the double-station self-holding electromagnet is fixedly arranged on the fixing plate, an output shaft of the double-station self-holding electromagnet is fixedly connected with the shifting fork metal plate, the shifting fork metal plate is rotationally connected with the fixing plate, and in operation, the swinging direction of the output shaft of the double-station self-holding electromagnet is adjusted by controlling the on-off of the double-station self-holding electromagnet, so that the shifting fork metal plate and the piercing member are mutually separated and separated, and the piercing member is controlled to be close to or far away from the bottom of the cleaning liquid cavity.
4. The vertical microorganism detecting apparatus according to claim 2, wherein the sealing film is provided on a surface of the first slide valve on which the flow passage is provided, the eluent chamber and the reaction liquid chamber are provided with rubber plugs, and the fixing plate in the longitudinal movement module is provided with two pressing rods for pressing the rubber plugs on the eluent chamber and the reaction liquid chamber respectively to press the wall of the eluent chamber and the wall of the reaction liquid chamber respectively to press the solution on the wall to the premixing chamber.
5. The vertical microorganism detecting apparatus according to claim 2, wherein in the longitudinal movement module, the motor is a second screw motor, the second screw motor is connected to the fixing plate through a first slider, the second screw motor is mounted on a mounting seat, the mounting seat is fixedly connected to a guide rail provided on the housing, and in operation, the second screw motor rotates to drive the fixing plate to linearly move downward along the guide rail.
6. The vertical microorganism detecting apparatus according to claim 2, wherein in the lateral movement module, a chip clamping and heating detecting module is disposed on the fixing frame, the chip clamping and heating detecting module is fixedly connected with the chip jig, and after the microfluidic chip is placed in a chip groove formed by the chip jig, the heating detecting module is aligned with and attached to a reaction chamber of the chip.
7. The vertical microorganism detection device according to claim 6, wherein the chip clamping and heating detection module comprises a first heating block fixing piece, a second heating block fixing piece and a pressing plate, wherein the first heating block fixing piece and the second heating block fixing piece are arranged on a shell through bearings and bearing fixing pieces, the heating blocks are fixedly arranged on the first heating block fixing piece and the second heating block fixing piece, the first heating block fixing piece and the second heating block fixing piece are movably connected through springs, a reaction chamber just falls into a groove formed between the first heating block fixing piece and the second heating block fixing piece after a microfluidic chip is placed in a chip jig, the pressing plate is hinged to the chip jig in a tilting mode relative to the left side and the right side, bosses which are parallel to and in butt joint with the pressing plate are arranged on one surface of the first heating block fixing piece, the bosses are arranged, and the planes of the two bosses are tilt planes and are matched with the planes of the pressing plate.
8. The vertical microorganism detection device according to claim 2, wherein in the transverse movement module, the first screw motor and the third screw motor drive the fixing frame and the push plate to linearly move respectively, the fixed end of the first screw motor is fixedly arranged on the shell, the driving end of the first screw motor is movably connected with the fixing frame, the fixed end of the third screw motor is arranged on the fixing frame, the driving end of the third screw motor is movably connected with the push plate, the guide rail is arranged on the fixing frame, the second slide block is arranged on the shell, in operation, the first screw motor drives the fixing frame to linearly move along the guide rail direction under the cooperation of the guide rail and the slide block to drive the chip jig to move out of and move into the shell, and the third screw motor drives the push plate to push the first slide valve and the second slide valve to linearly move.
9. The vertical microorganism detection apparatus according to claim 1, wherein the negative pressure pumping module comprises a first electromagnetic valve, a second electromagnetic valve and a step peristaltic pump, the step peristaltic pump is connected in parallel with the first electromagnetic valve and the second electromagnetic valve, the first electromagnetic valve is connected with one end of the first rigid hollow tubular column, and the second electromagnetic valve is connected with one end of the second rigid hollow tubular column, so that the first electromagnetic valve or the second electromagnetic valve is selectively opened during operation, and liquid is sequentially driven to flow.
10. A method of operating a vertical microorganism detection apparatus according to any one of claims 1 to 9, comprising the steps of: in the transverse movement module, the fixing frame transversely and linearly moves to drive the chip jig to move outside the shell to be placed into the microfluidic chip and move into the shell to enter an experimental state, and the push plate transversely and linearly moves relative to the fixing frame to push the first slide valve and the second slide valve to control the opening or closing of the first solution storage part, the second solution storage part and the reaction part; In the longitudinal movement module, the fixing plate moves downwards in a straight line to realize compression sealing between the end surfaces of the first rigid hollow tubular column, the second rigid hollow tubular column and the solid column in the microfluidic chip and the first negative pressure pumping channel, the second negative pressure pumping channel and the cracking liquid cavity corresponding to the microfluidic chip; In the negative pressure pumping module, the first electromagnetic valve or the second electromagnetic valve is selectively opened, and the step peristaltic pump sequentially drives liquid to flow.

Description

Vertical microorganism detection device and method Technical Field The invention relates to the technical field of microorganism detection, in particular to a vertical microorganism detection device and method. Background When the micro-fluidic chip is used for detecting microorganisms, sample splitting, cleaning, eluting and reaction steps can be integrated on one micro-fluidic chip. However, in order to prevent pollution, the structural design of the chamber and the flow channel of the existing microfluidic chip generally needs to adopt separate driving devices to pump the solutions into the target chamber, so that a plurality of driving devices are used in the whole detection process, resulting in large mechanical structure volume and high cost of the detection device, and the detection device is not portable, and is very inconvenient when the detection device needs to be moved to the outside for detection. Therefore, it is necessary to develop a microorganism detecting apparatus which is small in size, more compact in structure, and high in detection accuracy (using one pump, and capable of ensuring no occurrence of contamination). Disclosure of Invention Aiming at the defects of the prior art, the invention aims to provide a vertical microorganism detection device and a method with compact structure, small volume and high detection accuracy. The technical scheme of the invention is specifically introduced as follows. The vertical microorganism detection device comprises a longitudinal movement module, a transverse movement module, a negative pressure pumping module and a shell, wherein the longitudinal movement module is positioned above the transverse movement module, and in an initial state, the transverse movement module and the longitudinal movement module are positioned in the shell, and the vertical microorganism detection device comprises: The micro-fluidic chip is placed in a chip groove formed by the chip jig, the fixing frame is driven by a motor to move transversely and linearly so as to drive the chip jig to move outside the shell to be placed in the micro-fluidic chip and move into the shell to enter an experimental state, and the push plate is driven by the motor to move transversely and linearly relative to the fixing frame so as to realize the opening or closing of different solution storage cavities in the micro-fluidic chip; The longitudinal movement module comprises a first rigid hollow tubular column, a second rigid hollow tubular column, a solid column, a fixed plate and a first tension spring; the first rigid hollow pipe column, the second rigid hollow pipe column and the solid column are respectively provided with a first guide plate and a second guide plate which are used for guiding the hollow pipe column and the solid column to move in the vertical direction, the first rigid hollow pipe column, the second rigid hollow pipe column and the solid column are fixedly provided with clamps, one ends of the clamps and the first tension springs are fixedly connected, the other ends of the first tension springs are fixedly connected with the second guide plates, a fixing plate is arranged near the position of the clamps, and when the fixing plate is driven by a motor to move linearly downwards, the first tension springs are always in a stretching state and the fixing plate is in butt joint with the clamps, so that the first rigid hollow pipe column, the second rigid hollow pipe column and the solid column can provide enough compression force when contacting the top surface of the microfluidic chip, and the sealing between the end surfaces of the first rigid hollow pipe column, the second rigid hollow pipe column and the solid column in the microfluidic chip and the first negative pressure pumping channel, the second negative pressure pumping channel and the cracking liquid cavity corresponding to the microfluidic chip is realized; the negative pressure pumping module is used for orderly driving the liquid stored in the microfluidic chip to flow. The microfluidic chip comprises a first solution storage part, a first slide valve, a second solution storage part, a second slide valve and a reaction part which are sequentially arranged from top to bottom, wherein a cracking liquid cavity, a cleaning liquid cavity, an eluent cavity, a reaction liquid cavity, a first negative pumping pressure channel serving as a total pumping opening and a second negative pumping pressure channel serving as a sample pumping opening are arranged on the first solution storage part, a waste liquid cavity, a premixing cavity, a third channel and a fourth channel corresponding to the second negative pumping pressure channel are arranged in the second solution storage part, the reaction part is provided with the reaction cavity, a plurality of flow channels and through holes are arranged on the first slide valve so as to realize selective communication with solutions in the first solution storage part and the secon