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CN-121975609-A - Food-borne pathogenic bacteria detection device and detection method based on microfluidic chip

CN121975609ACN 121975609 ACN121975609 ACN 121975609ACN-121975609-A

Abstract

The invention discloses a food-borne pathogenic bacteria detection device and a detection method based on a microfluidic chip, comprising a shell, wherein a separation plate is fixedly connected in the shell, a setting cavity is arranged on the surface of the separation plate, a quick-dismantling mechanism for quickly dismantling a detection module is arranged in the setting cavity, the fixing mechanism comprises a spring arranged in the setting cavity, in the invention, an operator does not need to resort to any special tool by depending on the quick-dismantling mechanism driven by a pressing type inert gas, the disassembly and replacement of the core components such as the detection module, the power module and the like can be completed only through pressing and plugging, the whole process can be completed in a short time, the design effectively solves the problem that the existing integrated device needs to be returned to a factory for maintenance after a single module fails, and in the field detection scenes such as farmers markets, cold chain ports and the like, even if the problems such as blockage of the fluid driving module, failure of the temperature control module and the like occur, the detection work can be quickly recovered through replacement of the standby module, and the interruption of the detection flow is avoided.

Inventors

  • WANG JINGYI
  • YU LEMIN
  • LI QUAN
  • SHI YUBO
  • Lv Menzhe

Assignees

  • 沈阳农业大学

Dates

Publication Date
20260505
Application Date
20260206

Claims (9)

  1. 1. The utility model provides a food-borne bacteria detection device based on micro-fluidic chip, its characterized in that, including shell (1), the inside fixedly connected with division board (9) of shell (1), division board (9) surface has been seted up and has been set up chamber (18), set up the inside quick detach mechanism that is used for dismantling detection module (10) that is provided with of chamber (18), fixed establishment is including setting up in setting up spring (19) inside chamber (18), one end fixedly connected with sloping piece (17) of chamber (18) are kept away from to spring (19), one side fixed mounting of sloping piece (17) has extension strip (20), extension strip (20) and slider (21) are fixed to be linked to each other, slider (21) sliding connection has gas storage tube (22), gas storage tube (22) pipe connection has gas collection box (13), the inside fixedly connected with compression spring (14) of gas collection box (13), one end fixedly connected with pressing block (15) of gas collection box (13), pressing block (15) are kept away from compression spring (14) and are connected with each other with compression module (1), one side of detection module (11) is fixed to link up with each other, one end of the connecting plate (11) far away from the detection module (10) is fixedly connected with a hook block (16).
  2. 2. The food-borne bacteria detection device based on the microfluidic chip according to claim 1, wherein the gas storage tube (22) is fixedly connected with the setting cavity (18), the gas storage tube (22) is connected with the gas transmission tube (12) in a pipeline manner, and one end, far away from the gas storage tube (22), of the gas transmission tube (12) is fixedly connected with the gas collection box (13).
  3. 3. The food-borne bacteria detection device based on the microfluidic chip according to claim 1, characterized in that the shell (1) is slidably connected with the drawing box (3), one side of the shell (1) is provided with a stabilizing mechanism for supporting the drawing box (3), the stabilizing mechanism comprises a mounting frame (4) arranged on the side face of the shell (1), the mounting frame (4) is slidably connected with a sliding plate (5), the sliding plate (5) is fixedly connected with a sliding rod (7), one side of the sliding plate (5) is symmetrically provided with a compression spring (6), one end of the compression spring (6) away from the sliding plate (5) is fixedly connected with the mounting frame (4), one end of the sliding rod (7) penetrates through the mounting frame (4) to be used for stabilizing the drawing box (3), and one side of the sliding rod (7) away from the drawing box (3) is provided with a pull ring (8).
  4. 4. The food-borne bacteria detection device based on the microfluidic chip according to claim 1, wherein a top plate (2) is hinged above the housing (1), and a control panel for controlling the internal temperature of the drawing box (3) is arranged above the top plate (2).
  5. 5. The food-borne bacteria detection device based on the microfluidic chip according to claim 1, wherein a plurality of setting cavities (18) are formed on the surface of the isolation plate (9), and quick-release mechanisms inside the setting cavities (18) are used for fixing different modules.
  6. 6. The food-borne bacteria detection device based on the microfluidic chip according to claim 1, wherein a plurality of groups of bottom legs for buffering are arranged at the bottom of the housing (1).
  7. 7. The food-borne bacteria detection device based on the microfluidic chip according to claim 1, wherein inert gas for extrusion according to the pressing block (15) is arranged in the gas collection box (13).
  8. 8. The food-borne bacteria detection device based on the microfluidic chip according to claim 1, wherein a slope for pushing the slope block (17) is provided on the side of the hook block (16) away from the joint plate (11).
  9. 9. The method for using a microfluidic chip-based food-borne bacteria detection device according to claim 1, comprising the steps of: When a worker arrives at a detection area for monitoring food-borne pathogens, a top plate above a shell (1) is opened, then pretreated pathogen suspension is injected into a sample injection channel of a micro-fluidic chip through a micro-pump, a micro-channel network in the chip can guide sample liquid to flow according to a preset path so as to realize uniform distribution and quantitative transmission of samples, a reaction chamber surface of the chip is pre-coated with specific antibodies for target pathogens, such as anti-salmonella antibodies and anti-escherichia coli antibodies, when the sample liquid flows through the reaction chamber, if the target pathogens exist, antigens on the surface of the sample liquid can be combined with the antibodies on the chip to form an antigen-antibody immune complex; When the detection module (10) fails and cannot normally work, the top plate (2) is opened, the pressing block (15) corresponding to the gas collecting box (13) in the shell (1) is pressed by a finger, the pressing block (15) moves towards the inside of the gas collecting box (13) and compresses the compression spring (14), meanwhile, inert gas in the gas collecting box (13) is pressed into the gas storage tube (22) through a pipeline, the inert gas pressure in the gas storage tube (22) rises, the sliding bar (21) is pushed to move outwards, the sliding bar (21) drives the slope block (17) to synchronously move through the extension bar (20), the slope block (17) is separated from the clamping limit of the hook block (16), at the moment, the spring (19) is further compressed, the state of pressing the pressing block (15) is kept, the detection module (10) is lifted upwards, the connecting plate (11) and the hook block (16) are driven to be taken out from the inside of the setting cavity (18), the disassembly of the failure detection module (10) is completed, then the pressing block (15) is released, the compression spring (14) is released to drive the pressing block (15) to reset, meanwhile, the spring (19) pushes the slope block (17) to move synchronously, the slope block (17) to be pushed to the position, the sliding bar (20) is separated from the hook block (16) to the clamping limit, and the air in the air collecting box (18) is reset to the inside the setting up, the detection module (10) to be installed is picked up, the connecting plate (11) fixedly connected with the bottom of the detection module is aligned with the opening position of the setting cavity (18), the connecting plate (11) drives the hook block (16) to be vertically inserted into the setting cavity (18), in the inserting process, the inclined surface of one side of the hook block (16) far away from the connecting plate (11) is contacted with the slope block (17) and generates extrusion acting force, the slope block (17) is pushed to move towards the inner side of the setting cavity (18), meanwhile, the slope block (17) compresses the spring (19) to enable the elastic potential energy to be accumulated, the spring (19) continuously inserts into the detection module (10) downwards until the hook block (16) completely passes through the slope block (17), at the moment, the spring (19) releases the elastic potential energy, drives the slope block (17) to reset and is clamped and limited with the lower part of the hook block (16), the rapid fixing of the detection module (10) is completed, in the process, the slope block (17) is moved to drive the extension strip (20) and the sliding strip (21) to synchronously move, and the inner space of the gas storage tube (22) is adjusted adaptively, and the fixing of the hook block (16) is not affected.

Description

Food-borne pathogenic bacteria detection device and detection method based on microfluidic chip Technical Field The invention relates to the technical field of food-borne disease bacteria detection, in particular to a food-borne disease bacteria detection device and method based on a microfluidic chip. Background Food-borne pathogenic bacteria pollution is one of the core causes for triggering food safety events, pathogenic bacteria such as salmonella, escherichia coli and listeria can be transmitted through food matrixes such as meat, fruits and vegetables, dairy products and the like, the health of consumers is seriously threatened, salmonella is a typical food-borne pathogenic bacteria, mainly pollutes foods such as poultry meat, eggs and dairy products, the pathogenic mechanism is invasive infection, the pathogenic bacteria adhere to and invade intestinal mucosa epithelial cells after entering human intestinal tracts, pathogenic escherichia coli mainly pollutes raw beef, uncooked vegetables, drinking water and the like, the pathogenic core is shiga-like toxin production, listeria is a low-temperature-resistant pathogenic bacteria, and can grow and reproduce under refrigeration conditions, so that refrigerated meat products, dairy products, instant foods and the like are extremely easy to pollute. However, although the portable microfluidic detection device has advanced in miniaturization, the existing products mostly adopt an integrated structural design, and the lack of an independent split interface and a fixed structure between modules results in inconvenient disassembly and maintenance operations. In the field detection scenes of farmer markets, cold chain ports and the like, once a certain component such as a fluid driving module, a temperature control module and a detection probe is failed or blocked, a standby module cannot be quickly replaced on site, and the whole machine can only be returned to a factory for maintenance. The method not only can cause the interruption of detection work and prolong the period of food safety screening, but also can increase the maintenance cost and time cost of equipment, and is difficult to meet the requirements of field emergency detection on efficiency and continuity. Disclosure of Invention The invention aims to solve the defects in the technical problems in the prior art, and provides a food-borne pathogenic bacteria detection device and a detection method based on a microfluidic chip. In order to achieve the above purpose, the present invention adopts the following technical scheme: The utility model provides a food-borne disease bacteria detection device and detection method based on micro-fluidic chip, includes shell 1, the inside fixedly connected with division board 9 of shell 1, division board 9 surface has been seted up and has been set up chamber 18, set up the inside quick detach mechanism that is used for dismantling detection module 10 that is provided with of chamber 18, fixed establishment is including setting up the inside spring 19 in chamber 18, the one end fixedly connected with sloping block 17 of chamber 18 is kept away from to spring 19, one side fixedly mounted of sloping block 17 has extension strip 20, extension strip 20 links to each other with slider 21 is fixed, slider 21 sliding connection has gas storage tube 22, gas storage tube 22 pipe connection has gas collection box 13, the inside fixedly connected with compression spring 14 of gas collection box 13, compression spring 14 keeps away from the one end fixedly connected with pressing block 15 of gas collection box 13, pressing block 15 links to each other with compression spring 14 slip, gas collection box 13 links to each other with the inside fixedly of shell 1, one side fixedly connected with linking plate 11 of detection module 10, one end that detection module 11 kept away from detection module 10 has fixedly connected with hook 16. The technical scheme further comprises the following steps: The gas storage pipe 22 is fixedly connected with the setting cavity 18, the gas storage pipe 22 is connected with the gas transmission pipe 12 in a pipeline manner, and one end, far away from the gas storage pipe 22, of the gas transmission pipe 12 is fixedly connected with the gas collecting box 13. The shell 1 sliding connection has pull box 3, one side of shell 1 is provided with the stabilizing mean who is used for supporting pull box 3, stabilizing mean is including setting up the installing frame 4 at shell 1 side, installing frame 4 sliding connection has slide bar 5, slide bar 5 and slide bar 7 are fixed link to each other, one side symmetry of slide bar 5 is provided with extrusion spring 6, extrusion spring 6 keeps away from the one end fixedly connected with installing frame 4 of slide bar 5, the one end of slide bar 7 runs through installing frame 4 and is used for the stability to pull box 3, one side that slide bar 7 kept away from pull box 3 is provided with pull ring 8.