CN-121975610-A - Full-automatic nucleic acid amplification equipment

CN121975610ACN 121975610 ACN121975610 ACN 121975610ACN-121975610-A

Abstract

The invention provides full-automatic nucleic acid amplification equipment, which belongs to the technical field of nucleic acid detection and comprises a rack, wherein a deck plate is arranged in the rack, a system construction space and an amplification space are formed in the rack, a sealing transmission mechanism capable of being opened and closed is arranged on the deck plate, the sealing transmission mechanism is arranged as a material transmission channel between the system construction space and the amplification space, a sealing bin is arranged in the system construction space, a nucleic acid extraction module is arranged in the sealing bin, a bin door capable of being opened and closed is arranged in the sealing bin, the interior of the sealing bin is isolated from the system construction space when the bin door is closed, and the nucleic acid amplification module is arranged in the amplification space. The invention has the beneficial effects that the system construction space and the interior of the sealed bin are both set to be in a positive pressure environment, the air pressure of the system construction space is higher than the air pressure of the interior of the sealed bin, and the amplification space is set to be in a negative pressure environment, so that the whole process from reagent sample adding, system construction to PCR amplification can be completed in a closed system, the automation level of operation is improved, and the manual intervention is reduced.

Inventors

YU DING
LI YONGFU
LI YANG
He Xiantu
SHI FENGBING
WU XIAOHUI
XU ZHIWEN
TANG FENG
CHU ZHERONG

Assignees

宁波海尔施基因科技股份有限公司

Dates

Publication Date: 20260505
Application Date: 20260206

Claims (10)

1. A fully automated nucleic acid amplification apparatus comprising: The device comprises a rack (100), wherein a deck plate (110) is arranged in the rack (100), a system construction space (120) and an amplification space (130) are arranged in the rack (100), the system construction space (120) and the amplification space (130) are respectively positioned at the upper side and the lower side of the deck plate (110), the deck plate (110) is provided with an openable and closable sealing transmission mechanism, and the sealing transmission mechanism is arranged as a material transmission channel between the system construction space (120) and the amplification space (130); The sealed bin (200) is arranged in the system construction space (120), a nucleic acid extraction module is arranged inside the sealed bin (200), the sealed bin (200) is provided with a bin door (210) capable of being opened and closed, and the inside of the sealed bin (200) is isolated from the system construction space (120) when the bin door (210) is closed; a nucleic acid amplification module (300), the nucleic acid amplification module (300) being mounted to the amplification space (130); The system construction space (120) and the inside of the sealed bin (200) are both set to be in a positive pressure environment, the air pressure of the system construction space (120) is higher than the air pressure of the inside of the sealed bin (200), and the amplification space (130) is set to be in a negative pressure environment.
2. The fully automatic nucleic acid amplification apparatus as set forth in claim 1, wherein the system construction space (120) is provided with a first three-axis movement mechanism, a material conveying mechanism, a waste liquid sucking mechanism (400) and a film sealing mechanism (500), wherein the material conveying mechanism, the waste liquid sucking mechanism (400) and the film sealing mechanism (500) are all arranged on the table plate (110), the first three-axis movement mechanism is provided with a pipetting module, and the movable range of the first three-axis movement mechanism covers the movable range of the material conveying mechanism.
3. A full-automatic nucleic acid amplification apparatus according to claim 1 or 2, wherein the interior of the rack (100) further comprises at least three mutually independent cabinet cavities (140), each of the cabinet cavities (140) is located below the deck plate (110), the deck plate (110) is provided with a plurality of upper openings which are the same in number and are arranged in a one-to-one correspondence with the cabinet cavities (140), the cabinet cavities (140) are communicated with the system construction space (120) through the upper openings, the front panel of the rack (100) is provided with a plurality of front openings which are the same in number and are arranged in one-to-one correspondence with the cabinet cavities (140), the front openings are communicated with the cabinet cavities (140), the cabinet cavities (140) are provided with drawer cabinets (141) which can be pulled out from the front openings, when the drawer cabinets (141) are in a closed state, at least one of the drawer cabinets (141) is configured as a cabinet, at least one of the drawer cabinets (141) is configured as a sample cabinet, at least one of the sample cabinet is configured as a refrigerated cabinet, and at least one of the sample cabinet (141) is configured as a refrigerated cabinet.
4. A fully automatic nucleic acid amplification apparatus as set forth in claim 3, wherein the deck plate (110) is provided with a plurality of movable doors (111) which are provided in one-to-one correspondence with the upper openings, the upper openings are exposed when the movable doors (111) are opened, and the upper openings are closed when the movable doors (111) are closed.
5. The full-automatic nucleic acid amplification equipment according to claim 1, wherein an air inlet channel (121) and a ventilating duct (122) are installed in the frame (100), a first filter is arranged on an air inlet path in the air inlet channel (121), a second filter is arranged on an air exhaust path of the ventilating duct (122), an inlet of the air inlet channel (121) is located on a front panel of the frame (100), an outlet of the air inlet channel (121) is communicated with the top of the system construction space (120), an inlet of the ventilating duct (122) is located in the system construction space (120), and an exhaust gas discharge port of the sealed bin (200) is connected with the ventilating duct (122).
6. A fully automatic nucleic acid amplification apparatus according to claim 1, further comprising a centrifugation mechanism mounted in the amplification space (130), the centrifugation mechanism comprising a centrifugation cartridge (600) and a centrifugation module (640), the centrifugation module (640) being mounted in the centrifugation cartridge (600), the centrifugation module (640) having two centrifugation stations (641), both of the centrifugation stations (641) being located in the centrifugation cartridge (600), the centrifugation cartridge (600) being provided with a first port (610) and a second port (620), one of the two centrifugation stations (641) being aligned with the second port (620) when the other of the two centrifugation stations (641) is aligned with the first port (610).
7. A full-automatic nucleic acid amplification apparatus as set forth in claim 6, wherein the sealed transfer mechanism comprises a transfer chamber (700), the deck plate (110) is provided with a transfer opening (112), one end of the transfer chamber (700) is abutted with the transfer opening (112) and the other end is abutted with the first chamber opening (610) of the centrifugal chamber (600), a first transfer control door (113) capable of controlling the opening and closing of the transfer opening (112) is provided on the deck plate (110), a second transfer control door (630) capable of controlling the opening and closing of the second chamber opening (620) is provided on the centrifugal chamber (600), and the first transfer control door (113) and the second transfer control door (630) are interlocked so that both cannot be opened at the same time.
8. The apparatus of claim 7, wherein the amplification space (130) is provided with a second three-axis motion mechanism, and the range of motion of the second three-axis motion mechanism includes a region where the centrifugal mechanism is located and a region where the nucleic acid amplification module (300) is located.
9. A fully automatic nucleic acid amplification apparatus according to claim 1, wherein a negative pressure creating element (131) is further provided in the housing (100), an inlet of the negative pressure creating element (131) being connected to the amplification space (130), the negative pressure creating element (131) being arranged to draw air from the amplification space (130) to create a negative pressure environment in the amplification space (130).
10. A fully automatic nucleic acid amplification apparatus according to claim 3, wherein ultraviolet lamps are provided in both the system construction space (120) and the amplification space (130).

Description

Full-automatic nucleic acid amplification equipment Technical Field The invention belongs to the technical field of nucleic acid detection, and relates to full-automatic nucleic acid amplification equipment. Background Nucleic acid amplification technology (PCR) is a central tool in modern molecular diagnostics and life sciences research. The principle is that the DNA polymerase is utilized to carry out exponential amplification on specific DNA fragments in vitro through temperature cycle (denaturation, annealing and extension), and the trace target nucleic acid can be amplified for hundreds of hundred million times within a few hours, thereby being convenient for subsequent detection and analysis. In practice, the conventional PCR mode has a significantly short plate. Firstly, the operation is complicated and highly depends on manpower, the preparation (comprising the mixing of components such as a template, a primer, an enzyme, dNTPs and the like) of a reaction system, the sample addition and the transfer to a PCR instrument are carried out, the multiple steps are required to be completed manually, the efficiency is low, and the repeatability and the stability of the result are influenced by human errors. Secondly, the pollution risk is high, the PCR is extremely sensitive, the trace pollution can cause false positive, particularly when the product is taken after the amplification is finished, aerosol containing a large amount of amplified product is extremely easy to generate, and the specificity and the accuracy of subsequent experiments are seriously threatened after the aerosol is diffused to the environment. One complete PCR flow involves two key stages of PCR system construction and PCR amplification. The PCR instrument high temperature circulation in the amplification stage is easy to lead the reaction liquid to generate aerosol, and the process must be carried out in a negative pressure environment at this time, and potential pollution sources are sealed and discharged through directional airflow, thus preventing cross contamination. Based on the above requirements, the conventional scheme adopts a physically isolated independent partitioned laboratory as a PCR system construction region and a PCR amplification region. The mode has extremely high requirements on sites, ventilation systems, personnel lines and biological safety levels, and has high construction and operation and maintenance costs, thereby severely restricting the application of the PCR technology in space-limited scenes such as basic medical treatment, on-site quick inspection and the like. At present, two key steps of seamless connection system construction and amplification reaction in a closed system cannot be realized, and different stages of a PCR process need to be manually transferred between different sites, so that the full-automatic PCR process cannot be realized. Disclosure of Invention The present invention has been made in view of the above problems occurring in the prior art, and an object of the present invention is to provide a fully automatic nucleic acid amplification apparatus. The aim of the invention can be achieved by the following technical scheme that the full-automatic nucleic acid amplification equipment comprises: The device comprises a rack, wherein a deck plate is arranged in the rack, a system construction space and an amplification space are arranged in the rack, the system construction space and the amplification space are respectively positioned at the upper side and the lower side of the deck plate, the deck plate is provided with a sealing transmission mechanism capable of being opened and closed, and the sealing transmission mechanism is arranged as a material transmission channel between the system construction space and the amplification space; the sealed bin is arranged in the system construction space, a nucleic acid extraction module is arranged in the sealed bin, the sealed bin is provided with a bin door capable of being opened and closed, and the interior of the sealed bin is isolated from the system construction space when the bin door is closed; A nucleic acid amplification module mounted in the amplification space; the system construction space and the inside of the sealed bin are both set to be in a positive pressure environment, the air pressure of the system construction space is higher than the air pressure of the inside of the sealed bin, and the amplification space is set to be in a negative pressure environment. Preferably, the system construction space is provided with a first triaxial moving mechanism, a material conveying mechanism, a waste liquid absorbing mechanism and a film sealing mechanism, wherein the material conveying mechanism, the waste liquid absorbing mechanism and the film sealing mechanism are arranged on the table panel, the first triaxial moving mechanism is provided with a pipetting module, and the moving range of the first triaxial moving mechanism covers t