CN-121975611-A - Full-automatic nucleic acid amplification and electrophoresis analysis integrated equipment

CN121975611ACN 121975611 ACN121975611 ACN 121975611ACN-121975611-A

Abstract

The invention provides full-automatic nucleic acid amplification and electrophoresis analysis integrated equipment, which belongs to the technical field of nucleic acid detection and comprises a nucleic acid amplification operation platform, a nucleic acid extraction module, a system construction module, an electrophoresis detection module, a first frame internal air pressure and a third sealing cabin internal air pressure, wherein the nucleic acid amplification operation platform is arranged in a first sealing cabin, the nucleic acid amplification device is arranged in a second sealing cabin, the system construction module is arranged in a third sealing cabin, the electrophoresis detection module is arranged in a fourth sealing cabin, the first frame internal air pressure and the first sealing cabin internal air pressure are both set to be positive pressure environments higher than atmospheric pressure, and the second sealing cabin internal air pressure and the third sealing cabin internal air pressure are both set to negative pressure environments lower than atmospheric pressure.

Inventors

YU DING
LI YONGFU
LI YANG
He Xiantu
SHI FENGBING
WU XIAOHUI
XU ZHIWEN
TANG FENG
CHU ZHERONG

Assignees

宁波海尔施基因科技股份有限公司

Dates

Publication Date: 20260505
Application Date: 20260206

Claims (10)

1. A fully automated nucleic acid amplification and electrophoresis analysis integrated apparatus comprising: The nucleic acid amplification operation platform comprises a first rack (100), a nucleic acid extraction module and a nucleic acid amplification device, wherein a first sealing bin (110) and a second sealing bin (120) are arranged in the first rack (100), the nucleic acid extraction module is arranged in the first sealing bin (110), and the nucleic acid amplification device is arranged in the second sealing bin (120); The electrophoresis analysis operation platform comprises a second rack (200), a system construction module and an electrophoresis detection module, wherein a third sealing bin (210), a fourth sealing bin (220) and a waste bin (230) are arranged in the second rack (200), the system construction module is arranged in the third sealing bin (210), and the electrophoresis detection module is arranged in the fourth sealing bin (220); A transfer module having one end in abutment with the second sealed compartment (120) and the other end in abutment with the third sealed compartment (210), the transfer module being configured to transfer nucleic acid amplification products treated by the nucleic acid amplification apparatus to the system construction module; The first rack (100), the second rack (200), the first sealed bin (110), the second sealed bin (120), the third sealed bin (210) and the fourth sealed bin (220) are all arranged to be of a closable independent space structure, the internal air pressure of the first rack (100) and the internal air pressure of the first sealed bin (110) are all arranged to be in a positive pressure environment higher than the atmospheric pressure, and the internal air pressure of the second sealed bin (120) and the internal air pressure of the third sealed bin (210) are all arranged to be in a negative pressure environment lower than the atmospheric pressure.
2. The integrated apparatus for full-automatic nucleic acid amplification and electrophoresis analysis according to claim 1, wherein the air pressure inside the first housing (100), the air pressure inside the first sealed chamber (110), the air pressure inside the second sealed chamber (120), and the air pressure inside the third sealed chamber (210) are sequentially decreased.
3. The integrated full-automatic nucleic acid amplification and electrophoresis analysis device according to claim 1, wherein the first rack (100) is provided with a sample bin (130) and at least one first material bin (140), the sample bin (130) and the first material bin (140) are both arranged in a closable independent space structure, the sample bin (130) is provided with a first bin door (131) capable of being opened and closed, the first material bin (140) is provided with a second bin door (141) capable of being opened and closed, and the first bin door (131) and the second bin door (141) are both positioned on a table top of the first rack (100).
4. The full-automatic nucleic acid amplification and electrophoresis analysis integrated equipment according to claim 1 or 3, wherein the second rack (200) is provided with a second storage bin (240), the second storage bin (240) is provided with a closable independent space structure, a rotating platform (241) is arranged in the second storage bin (240), the rotating platform (241) is provided with a plurality of consumable supports arranged along the circumference of the rotating platform, the second storage bin (240) is provided with a third storage door (242) capable of being opened and closed, the third storage door (242) is positioned at the bottom of the third sealing storage bin (210), and the third sealing storage bin (210) is communicated with the second storage bin (240) when the third storage door (242) is opened.
5. The integrated full-automatic nucleic acid amplification and electrophoresis analysis device according to claim 1, wherein the first sealing chamber (110) is provided with a positive pressure establishing element and an openable fourth chamber door (111), the positive pressure establishing element is configured to inject air in the first housing (100) into the first sealing chamber (110) so as to establish a positive pressure environment in the first sealing chamber (110), and the fourth chamber door (111) is opened so that the first sealing chamber (110) is communicated with the interior of the first housing (100).
6. The integrated full-automatic nucleic acid amplification and electrophoresis analysis device according to claim 1, wherein the first rack (100) comprises an operation area above a table top and an amplification area below the table top, the first sealed cabin (110) is located in the operation area, the second sealed cabin (120) is located in the amplification area, the nucleic acid amplification operation platform further comprises a first transfer cabin (150) and a second transfer cabin, the first transfer cabin (150) and the second transfer cabin are located in the second sealed cabin (120), the first transfer cabin (150) and the second transfer cabin are both in a closable independent space structure, one end of the first transfer cabin (150) is provided with a fifth cabin door (151) located on the table top of the first rack (100), the first transfer cabin (150) is communicated with the operation area when the fifth cabin door (151) is opened, one end of the second transfer cabin (150) and the other end of the second transfer cabin (161) are provided with a sixth transfer cabin, and the second transfer cabin (161) are in butt joint.
7. The integrated full-automatic nucleic acid amplification and electrophoresis analysis device according to claim 6, wherein the second sealing bin (120) is provided with a negative pressure building element (121), the negative pressure building element (121) is arranged to extract air of the second sealing bin (120) so as to build a negative pressure environment inside the second sealing bin (120), a centrifugal bin body (122) is arranged in the second sealing bin (120), the centrifugal bin body (122) is arranged to be of a closable independent space structure, the other end of the first transfer bin (150) is in butt joint with the centrifugal bin body (122), the centrifugal bin body (122) is provided with a seventh bin gate (123), and the centrifugal bin body (122) is communicated with the second sealing bin (120) when the seventh bin gate (123) is opened.
8. The integrated full-automatic nucleic acid amplification and electrophoresis analysis device according to claim 7, wherein the nucleic acid amplification device comprises a centrifugal device (300) and at least one nucleic acid amplification module (400), the centrifugal device (300) is arranged on the centrifugal bin body (122), and the nucleic acid amplification module (400) is arranged on the second sealed bin (120).
9. The integrated full-automatic nucleic acid amplification and electrophoresis analysis device according to claim 7, wherein the transfer module comprises a guide rail (510), a sliding block (520), a driving element (540) and a track groove (550), two ends of the guide rail (510) are respectively in butt joint with the second transfer bin and the third sealing bin (210), the sliding block (520) is in sliding connection with the guide rail (510), the driving element (540) is connected with the sliding block (520) and can drive the sliding block (520) to slide along the guide rail (510), the sliding block (520) is provided with a rotatable follow-up seat (530), the follow-up seat (530) is provided with a driven rod (531), the driven rod (531) is connected with the track groove (550), and when the sliding block (520) slides from one end of the guide rail (510) to the other end, the shape of the track groove (550) forces the driven rod (531) to drive the follow-up seat (530) to rotate.
10. The integrated full-automatic nucleic acid amplification and electrophoresis analysis device according to claim 1, wherein ultraviolet lamps are arranged in the first frame (100), the second sealed cabin (120) and the third sealed cabin (210).

Description

Full-automatic nucleic acid amplification and electrophoresis analysis integrated equipment Technical Field The invention belongs to the technical field of nucleic acid detection, and relates to full-automatic nucleic acid amplification and electrophoresis analysis integrated equipment. Background Nucleic acid amplification (PCR) and Capillary Electrophoresis (CE) together constitute the core support of modern nucleic acid detection technology systems. The PCR technology can carry out exponential amplification on specific deoxyribonucleic acid fragments in vitro, the DNA polymerase is utilized to copy and amplify very trace target gene fragments for hundreds of millions of times within a few hours through specific temperature circulation, so that trace nucleic acids which cannot be detected originally become easy to analyze, and the CE technology can realize efficient separation of nucleic acid molecules with different sizes and charges in a tube by applying a high-voltage electric field to two ends of a capillary filled with a screening medium, and qualitatively and quantitatively analyze the separated components in a fluorescent detection mode and the like. The combination of PCR amplification technology and capillary electrophoresis detection technology provides an effective scheme for the accurate amplification and detection of specific nucleic acid sequences. However, in actual detection, the process is highly dependent on manual operation, so that the operation steps are complicated, the result stability is poor, and each process is easy to generate high-risk aerosol pollution or sample pollution. Meanwhile, in order to avoid pollution, an independent partition laboratory scheme is adopted conventionally, and strict requirements are put on the field scale and the biological safety level. At present, although full-automatic nucleic acid extraction amplification equipment or automatic electrophoresis detection equipment exists in the market, the full-automatic nucleic acid extraction amplification equipment and the automatic electrophoresis detection equipment are single-function equipment, and only PCR amplification or CE detection can be realized, so that one-stop full-flow detection of PCR-CE can not be finished. The root cause is that in the actual detection link, different procedures have essential conflicts on the air pressure control and pollution prevention standards, so that the existing equipment is difficult to integrate the whole PCR-CE process. Disclosure of Invention The invention aims at solving the problems existing in the prior art and provides full-automatic integrated equipment for nucleic acid amplification and electrophoresis analysis. The invention aims at realizing the following technical scheme that the full-automatic integrated equipment for nucleic acid amplification and electrophoresis analysis comprises the following components: The nucleic acid amplification operation platform comprises a first rack, a nucleic acid extraction module and a nucleic acid amplification device, wherein a first sealing bin and a second sealing bin are arranged in the first rack, the nucleic acid extraction module is arranged in the first sealing bin, and the nucleic acid amplification device is arranged in the second sealing bin; The electrophoresis analysis operation platform comprises a second frame, a system construction module and an electrophoresis detection module, wherein a third sealing bin, a fourth sealing bin and a waste bin are arranged in the second frame, the system construction module is arranged in the third sealing bin, and the electrophoresis detection module is arranged in the fourth sealing bin; a transfer module having one end thereof docked with the second sealed compartment and the other end thereof docked with the third sealed compartment, the transfer module being configured to transfer the nucleic acid amplification product treated by the nucleic acid amplification apparatus to the system construction module; the first frame, the second frame, the first sealed bin, the second sealed bin, the third sealed bin and the fourth sealed bin are all arranged to be of a closeable independent space structure, the internal air pressure of the first frame and the internal air pressure of the first sealed bin are all arranged to be positive pressure environments higher than atmospheric pressure, and the internal air pressure of the second sealed bin and the internal air pressure of the third sealed bin are all arranged to be negative pressure environments lower than atmospheric pressure. Preferably, the air pressure inside the first frame, the air pressure inside the first sealed cabin, the air pressure inside the second sealed cabin and the air pressure inside the third sealed cabin are sequentially reduced. Preferably, the first rack is provided with a sample bin and at least one first material bin, the sample bin and the first material bin are both arranged to be of a closable inde