CN-121975614-A - Preparation device and method for preparing traditional Chinese medicine active ingredients by utilizing enzyme-enzyme coupling extraction

CN121975614ACN 121975614 ACN121975614 ACN 121975614ACN-121975614-A

Abstract

The invention discloses a preparation device and a preparation method for preparing active ingredients of traditional Chinese medicine by utilizing enzyme-fermentation coupling extraction, belonging to the field of traditional Chinese medicine preparation; the device comprises a liquid conveying mechanism and a solid conveying mechanism, wherein the liquid conveying mechanism comprises a first cylinder for containing a compound enzyme solution, a second cylinder for containing a biological strain culture solution, a first peristaltic pump and a second peristaltic pump, wherein the first peristaltic pump and the second peristaltic pump are connected with the first cylinder and the second cylinder, a first piston plate and a second piston plate are respectively connected in the first cylinder and the second cylinder in a sliding manner, a support is fixedly connected with the first piston plate and the second piston plate together, the solid conveying mechanism comprises a plurality of conveying belts for outputting raw materials and auxiliary materials, the first peristaltic pump and the second peristaltic pump can drive the support to move downwards, the support can drive the conveying belts to move at different speeds after moving downwards, and the device can automatically put materials with proper quantity and proportion according to the set and adjustment.

Inventors

DONG XIAODAN
PENG CHUNE
WANG HENGZHEN
PENG LIZENG
SUN SUJUN
DENG PENG
LIANG XIAOHUI
SI HONGYU
Jiao Jianshuang
JIA XIUWEN
WANG XINKUN

Assignees

山东省农业科学院

Dates

Publication Date: 20260505
Application Date: 20260209

Claims (10)

1. The preparation device for preparing the traditional Chinese medicine active ingredients by utilizing enzyme-enzyme coupling extraction is characterized by comprising a liquid conveying mechanism (100) and a solid conveying mechanism (200), wherein the liquid conveying mechanism (100) comprises a first cylinder (120) for containing a compound enzyme solution, a second cylinder (130) for containing a biological strain culture solution, a first peristaltic pump (150) and a second peristaltic pump (160) which are connected with the first cylinder (120) and the second cylinder (130), a first piston plate (121) and a second piston plate (131) are respectively and slidingly connected with the first cylinder (120) and the second cylinder (130), and a bracket (140) is fixedly connected with the first piston plate (121) and the second piston plate (131) together; The solid conveying mechanism (200) comprises a plurality of conveying belts (220) for outputting raw materials and auxiliary materials, the first peristaltic pump (150) and the second peristaltic pump (160) can drive the support (140) to move downwards, and the support (140) can drive the conveying belts (220) to move at different speeds after moving downwards.
2. The apparatus for preparing a Chinese medicinal active ingredient by enzymatic coupling extraction according to claim 1, wherein the inner diameter ratio of the first cylinder (120) to the second cylinder (130) is 1:2 to 1:5; The pump shafts of the first peristaltic pump (150) and the second peristaltic pump (160) are respectively connected with a first gear (151) and a second gear (161), the first gear (151) and the second gear (161) are meshed with each other, the diameter ratio is 2:1 to 16:1, and any pump shaft is fixedly connected to the output end of an external motor.
3. The device for preparing active ingredients of traditional Chinese medicine by enzyme-ferment coupling extraction as claimed in claim 2, further comprising a fermentation mechanism (300), wherein the fermentation mechanism (300) comprises a fermentation cylinder (310), the liquid outlet ends of the first peristaltic pump (150) and the second peristaltic pump (160) are jointly communicated with an infusion tube (170), and the infusion tube (170) is communicated with the fermentation cylinder (310).
4. The device for preparing active ingredients of Chinese medicine by enzyme-ferment coupling extraction according to claim 3, wherein the solid conveying mechanism (200) further comprises a plurality of conveying frames (210) fixedly arranged, a plurality of conveying belts (220) are respectively connected with the conveying frames (210) in a transmission way, a plurality of feeding hoppers (221) are connected to the fermentation cylinder (310), and the feeding hoppers (221) are respectively positioned at the lower parts of the discharging ends of the conveying belts (220); The upper parts of the conveying frames (210) positioned on the conveying belts (220) are fixedly connected with feeding hoppers (240), and the feeding hoppers (240) are respectively used for containing raw materials and auxiliary materials.
5. The apparatus for preparing a Chinese medicinal active ingredient by enzymatic coupling extraction as set forth in claim 4, wherein a plurality of the carriers (210) are each provided with a scraping plate (230), and the scraping plates (230) flatten the material when the material is conveyed by the conveyor belt (220).
6. The device for preparing the active ingredients of the traditional Chinese medicine by utilizing the enzyme-fermentation coupling extraction as set forth in claim 5, wherein a rectangular plate (260) is installed on the bracket (140), a plurality of groups of teeth are arranged on the rectangular plate (260), a plurality of rotating rollers (250) for driving the conveyor belt (220) to rotate are rotatably connected on the conveying frames (210), a plurality of transmission teeth (251) meshed with the plurality of groups of teeth are fixedly connected on the rotating rollers (250), and the rectangular plate (260) can drive the conveyor belt (220) to operate when moving downwards.
7. The device for preparing the active ingredients of the traditional Chinese medicine by utilizing the enzyme-fermentation coupling extraction of claim 6, wherein the top of the rectangular plate (260) is fixedly connected with a vertical rod (270), the vertical rod (270) is axially and slidably connected to the bracket (140), a first spring (271) is connected between the rectangular plate (260) and the bracket (140), the fermentation cylinder (310) is fixedly connected with a mounting frame (110), the mounting frame (110) is fixedly connected with a vertical plate (111), the vertical plate (111) is inserted with a telescopic rod (290), the end part of the telescopic rod (290) can be abutted to the rectangular plate (260), and when the telescopic rod (290) is far away from the rectangular plate (260), the first spring (271) is released elastically, so that the conveyor belt (220) is driven to operate.
8. The device for preparing the active ingredients of the traditional Chinese medicine by utilizing the enzyme-fermentation coupling extraction of claim 7, wherein the fermentation mechanism (300) further comprises a base (340), a driving motor (320) is fixedly connected to the base (340), a rotating shaft (330) is fixedly connected to the output end of the driving motor (320), and a stirring rod (331) is fixedly connected to the rotating shaft (330).
9. The device for preparing the active ingredients of the traditional Chinese medicine by utilizing the enzyme-fermentation coupling extraction as set forth in claim 8, wherein the base (340) is axially and slidably connected with a mounting rod (281), the middle part of the mounting rod (281) is fixedly connected with a rectangular frame (280), the rotating shaft (330) is inserted into the rectangular frame (280), a protrusion (282) is arranged on the side wall of the rotating shaft (330), and the rotating shaft (330) can drive the protrusion (282) to alternately push two symmetrical inner walls of the rectangular frame (280) when rotating, so that the rectangular frame (280) axially slides back and forth along the mounting rod (281); the utility model discloses a fermentation cylinder (310) of fermentation, including flexible pole (290) and riser (111), rectangle frame (280) are connected with between flexible pole (290) with fixedly connected with second spring (291) between riser (111), rectangle frame (280) orientation the one side of flexible pole (290) is the inclined plane, the tip of flexible pole (290) with the laminating of inclined plane, rectangle frame (280) can be driven when reciprocating sliding rectangular plate (260) intermittent type moves down to make raw materials and auxiliary material intermittent type release arrive fermentation cylinder (310).
10. A preparation method for preparing traditional Chinese medicine active ingredients by utilizing enzyme-ferment coupling extraction, which adopts the preparation device for preparing traditional Chinese medicine active ingredients by utilizing enzyme-ferment coupling extraction according to claim 9, and is characterized by comprising the following steps: material preassembly: injecting a special compound enzyme solution for enzyme fermentation of traditional Chinese medicine into a first cylinder (120), and injecting a biological strain culture solution into a second cylinder (130); respectively filling dry traditional Chinese medicine materials and fermentation auxiliary materials into a plurality of charging hoppers (240), ensuring that the materials cover a conveyor belt (220), and pre-adjusting a scraping plate (230) to a proper height; The parameter presetting comprises the steps of setting the inner diameter ratio of a first cylinder (120) and a second cylinder (130) and the diameter ratio of a first gear (151) and a second gear (161) according to the enzyme fermentation requirement of a target traditional Chinese medicine, and ensuring the accurate conveying proportion of enzyme liquid and bacterial liquid; starting feeding: starting an external motor, driving the first peristaltic pump (150) and the second peristaltic pump (160) to operate, and conveying enzyme liquid and bacterial liquid to the fermentation cylinder (310) through the infusion tube (170); The first peristaltic pump (150) and the second peristaltic pump (160) drive the bracket (140) to move downwards when running, the bracket (140) moves downwards to drive the rectangular plate (260) to move downwards synchronously, and a plurality of groups of teeth on the rectangular plate (260) are meshed with the transmission teeth (251) of the rotating roller (250), so that a plurality of conveyor belts (220) are driven to run at different speeds; When the conveyor belt (220) operates, the scraping plate (230) spreads the dry traditional Chinese medicine materials and auxiliary materials to ensure uniform discharging, and the materials accurately fall into the fermentation cylinder (310) through the feeding hopper (221) to realize the cooperative feeding of enzyme liquid, bacterial liquid, traditional Chinese medicine materials and auxiliary materials; Enzyme-enzyme coupling reaction: the synchronous coupling mode is that enzyme liquid, bacterial liquid and raw materials synchronously enter a fermentation cylinder (310), a driving motor (320) is started, a rotating shaft (330) drives a stirring rod (331) to rotate, so that the materials are fully mixed, the temperature in the fermentation cylinder (310) is maintained at 30-55 ℃, and the oxygen environment of the fermentation cylinder (310) is regulated according to target raw materials; the enzymolysis stage, wherein the complex enzyme solution damages the cell wall of the traditional Chinese medicine, reduces mass transfer resistance and enables active ingredients to be primarily dissolved out; In the fermentation stage, enzyme systems generated by metabolism of biological strains are further subjected to synergistic enzymolysis, and impurities such as starch, protein and the like in the traditional Chinese medicine are degraded, so that active ingredients are enriched; post-treatment: After the enzyme-ferment coupling reaction is completed, all power mechanisms are closed, fermentation liquor is discharged from a fermentation cylinder (310), dregs and extracting solution are separated, active ingredients in the extracting solution are fully dissolved out, the purity is improved, and the extracting solution can directly enter a subsequent purification process.

Description

Preparation device and method for preparing traditional Chinese medicine active ingredients by utilizing enzyme-enzyme coupling extraction Technical Field The invention relates to the technical field of traditional Chinese medicine preparation devices, in particular to a preparation device and a preparation method for preparing traditional Chinese medicine active ingredients by utilizing enzyme-enzyme coupling extraction. Background The active ingredients of the traditional Chinese medicine (such as flavone, polysaccharide, alkaloid, volatile oil, etc.) are the core material basis of the drug effect of the traditional Chinese medicine, and the efficient extraction and full dissolution of the active ingredients are key links of the modern processing of the traditional Chinese medicine. The traditional Chinese medicine active ingredient extraction method (such as hot water extraction, organic solvent extraction, reflux extraction and the like) has remarkable limitations that on one hand, the cell walls of Chinese medicine raw materials (such as roxburgh rose, sweet wormwood, perilla, mulberry leaves and the like) are composed of dense structures of cellulose, pectin, hemicellulose and the like to form a natural mass transfer barrier, so that active ingredients are difficult to fully release from cells, the extraction rate is low, and on the other hand, the traditional process usually depends on high temperature, high pressure or a large amount of organic solvents, so that the heat-sensitive and easily-oxidized active ingredients (such as vitamin C, artemisinin and volatile oil) are easily damaged, the biological activity of products is reduced, and the problems of high energy consumption, high environmental protection pressure, low product purity and the like are also caused. In order to solve the pain spots, the enzyme-ferment coupling extraction technology gradually becomes a preferred scheme for preparing the active ingredients of the traditional Chinese medicine. The technology degrades the cell wall structure of the traditional Chinese medicine by the compound enzyme (such as cellulase and pectase), reduces mass transfer resistance, enriches active ingredients by utilizing the fermentation action of biological strains (such as saccharomycetes, aspergillus niger and bacillus subtilis), converts macromolecular substances into high-activity micromolecules, degrades impurities such as starch, protein and the like, and realizes the efficient dissolution and quality improvement of the active ingredients. However, in the existing enzyme-ferment coupling extraction process, accurate configuration and dynamic regulation of materials are inconvenient; For example, the cell wall composition and the active ingredient characteristic of different traditional Chinese medicine raw materials are obviously different, and the ratio requirements on the complex enzyme solution and the biological strain culture solution are different (for example, high-proportion pectase is required for the extraction of roxburgh rose, and the extraction of sweet wormwood is required to be matched with a specific fermentation strain). The existing equipment adopts manual feeding or a single conveying channel, so that accurate quantitative conveying of enzyme liquid and bacterial liquid is difficult to realize, and the ratio deviation easily causes insufficient enzymolysis and unbalanced bacterial metabolism, thereby affecting the dissolution efficiency of active ingredients. Disclosure of Invention The invention aims to provide a preparation device and a preparation method for preparing traditional Chinese medicine active ingredients by utilizing enzyme-enzyme coupling extraction, which solve the problems that the proportion and the input amount are not easy to control by manual feeding. The preparation device comprises a liquid conveying mechanism and a solid conveying mechanism, wherein the liquid conveying mechanism comprises a first cylinder for containing a compound enzyme solution, a second cylinder for containing a biological bacterial culture solution, a first peristaltic pump and a second peristaltic pump which are connected with the first cylinder and the second cylinder, a first piston plate and a second piston plate are respectively and slidably connected in the first cylinder and the second cylinder, and a bracket is fixedly connected with the first piston plate and the second piston plate together; The solid conveying mechanism comprises a plurality of conveying belts for outputting raw materials and auxiliary materials, the first peristaltic pump and the second peristaltic pump can drive the support to move downwards, and the support can drive the conveying belts to move at different speeds after moving downwards. Preferably, the inner diameter ratio of the first cylinder to the second cylinder is 1:2 to 1:5; The pump comprises a first peristaltic pump, a second peristaltic pump, a first gear, a second gear, a diameter ratio of