CN-121975616-A - Low-damage electrotransport device suitable for NK cells and T cells

CN121975616ACN 121975616 ACN121975616 ACN 121975616ACN-121975616-A

Abstract

The invention discloses a low-damage electrotransport device suitable for NK cells and T cells, which comprises a high-voltage pulse module, an electrode module, a double-liquid conveying device and a liquid mixing chamber, wherein the liquid mixing chamber comprises a stirring shaft arranged in the middle of the liquid mixing chamber, stirring paddles arranged on the stirring shaft, a stirring accelerating plate spirally arranged on the outer ring of the stirring paddles and extending from the top to the bottom of the liquid mixing chamber, and a telescopic ring annularly arranged on the outer side of the stirring accelerating plate and axially sliding along the liquid mixing chamber, and the telescopic ring axially displaces along the liquid mixing chamber to adjust the volume in the liquid mixing chamber. The low-damage electrotransport converter suitable for NK cells and T cells provided by the invention relies on a three-layer mixing structure of a stirring paddle basic flow field, a stirring accelerating plate spiral flow field and a fish bone type barrier rib local turbulence, so that two liquids are fully contacted in the flowing process, the mixing uniformity is good, no intense stirring is caused, and the breakage rate of NK/T cells caused by shearing force is reduced by 70%.

Inventors

MA RUBIAO
XU ZHIFENG
LIU GUIBO
ZHANG XIN

Assignees

广州沙艾生物科技有限公司

Dates

Publication Date: 20260505
Application Date: 20260204

Claims (10)

1. The low-damage electrotransport device suitable for NK cells and T cells comprises a high-voltage pulse module and an electrode module, and is characterized by further comprising a double-liquid conveying device and a liquid mixing chamber connected to the double-liquid conveying device, wherein the double-liquid conveying device comprises a first liquid conveying device and a second liquid conveying device, the liquid mixing chamber comprises a first connecting pipe at the top, a second connecting pipe at the top, a stirring shaft, a stirring paddle, a stirring accelerating plate, a telescopic ring and a telescopic ring, the first connecting pipe is connected with the first liquid conveying device, the second connecting pipe is connected with the second liquid conveying device, the stirring shaft is arranged in the middle of the liquid mixing chamber, the stirring paddle is arranged on the stirring shaft, the stirring accelerating plate is spirally arranged on the outer ring of the stirring paddle and extends from the top to the bottom of the liquid mixing chamber, the telescopic ring is annularly arranged outside the stirring accelerating plate and axially slides along the liquid mixing chamber, and the bottom of the telescopic ring is connected with the telescopic device, and the telescopic ring axially displaces along the liquid mixing chamber to adjust the volume in the liquid mixing chamber.
2. The low-damage electrotransport device for NK cells and T cells of claim 1, wherein the top of the telescopic ring is configured as a beveled annular surface.
3. The low-damage electrotransport device for NK cells and T cells according to claim 1, wherein the stirring acceleration plate is provided with a fish-bone barrier.
4. The low-damage electrotransport device for NK cells and T cells of claim 1, wherein the fishbone barrier is horizontal or diagonal.
5. The low-damage electrotransport device for NK cells and T cells of claim 1, wherein a plurality of fishbone bars are provided.
6. The low-damage electrotransport device for NK cells and T cells according to claim 1, wherein the bottom of the liquid mixing chamber is provided with a funnel-shaped discharge port.
7. The low-damage electrotransport device for NK cells and T cells according to claim 1, further comprising a stationary post extending from the top to the bottom of the liquid mixing chamber fixedly connected to the agitation accelerator plate.
8. The low-damage electrotransport device for NK cells and T cells of claim 1 wherein said stirring shaft top is connected to a stirring device.
9. The low-damage electrotransport device for NK cells and T cells according to claim 1, wherein said telescopic means is provided in two.
10. The low-damage electrotransport device for NK cells and T cells of claim 1, wherein said telescoping device is a telescoping cylinder.

Description

Low-damage electrotransport device suitable for NK cells and T cells Technical Field The invention relates to the field of electrotransport devices, in particular to a low-damage electrotransport device suitable for NK cells and T cells. Background The electrotransformation instrument is experimental equipment which enables cell membranes to form instantaneous micropores through the action of a short-time high-voltage pulse electric field on biological materials such as cells, bacteria, plant protoplasts and the like, thereby realizing the efficient introduction of exogenous substances such as DNA, RNA, protein, drug molecules, nano materials and the like. The core principle is that an electric field damages the stability of a phospholipid bilayer of a cell membrane to form a reversible channel, the cell membrane is repaired by itself after the electric field disappears, and exogenous substances are trapped in the cell. For NK cells and T cells, the mixing ratio of the cells and the protective liquid needs to be accurately controlled when an electrotransport device is used, and cell damage caused by excessive stirring needs to be avoided, and electric field distortion or cell damage caused by air bubbles entering an electrode area needs to be prevented. However, the existing electrotransport devices are usually stirred manually or by the existing stirrer, so that bubbles are easy to generate and cells are damaged as a result of stirring, and the influence on the subsequent electrotransport device experiment is large. Disclosure of Invention The invention aims to solve the defects in the prior art and provides a low-damage electrotransport device which can realize uniform liquid mixing and reduce the existence of bubbles and cell damage and is suitable for NK cells and T cells. The invention firstly provides a low-damage electrotransport device suitable for NK cells and T cells, which comprises a high-voltage pulse module, an electrode module, a double-liquid conveying device and a liquid mixing chamber connected to the double-liquid conveying device, wherein the double-liquid conveying device comprises a first liquid conveying device and a second liquid conveying device, the liquid mixing chamber comprises a first connecting pipe at the top part connected with the first liquid conveying device, a second connecting pipe at the top part connected with the second liquid conveying device, a stirring shaft arranged in the middle of the liquid mixing chamber, a stirring paddle arranged on the stirring shaft, a stirring accelerating plate spirally arranged on the outer ring of the stirring paddle and extending from the top part to the bottom part of the liquid mixing chamber, and a telescopic ring annularly arranged on the outer side of the stirring accelerating plate and axially sliding along the liquid mixing chamber, and the bottom part of the telescopic ring is connected with the telescopic device and axially displaces along the liquid mixing chamber to adjust the volume in the liquid mixing chamber. The top of the stirring shaft penetrates out of a central hole of a cover plate at the top of the liquid mixing chamber and is coaxially connected with the stirring device above, the blades of the stirring paddles on the stirring shaft form a certain included angle with the shaft, and preferably, the stirring shaft can be provided with a temperature sensor which is connected to a main control module of the electrotransport device. Preferably, the blade may be provided as an angle-adjustable blade, the angle of which may be adjusted according to the cell type. The telescopic ring is sleeved between the inner wall of the mixing chamber and the stirring accelerating plate, and the bottom of the telescopic ring is connected with the telescopic device. The invention also provides the following optimization scheme: Preferably, the top of the telescopic ring is provided with an inclined annular surface. The top of the telescopic ring is not a horizontal annular surface, but is designed into an inclined surface inclined towards the inner ring to form a circle of continuous inclined annular contact surface. Preferably, the stirring accelerating plate is provided with a fishbone type baffle bar. The fishbone type baffle on the surface of the stirring acceleration plate is preferably provided as a cylindrical strip-shaped plate. The fish bone type barrier strip is arranged on the surface of the stirring accelerating plate, along the extending direction of the stirring accelerating plate, is similar to the protruding structure of a fish bone, a plurality of short branch strip-shaped plates are uniformly distributed on one side or two sides of the stirring accelerating plate, and the whole stirring accelerating plate is integrally formed or fixedly connected with the stirring accelerating plate. Preferably, the fishbone type barrier is horizontal or inclined. The horizontal fishbone baffle strip extends horizontally, the incli