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CN-121975617-A - Stem cell culture carrier input device and use method

CN121975617ACN 121975617 ACN121975617 ACN 121975617ACN-121975617-A

Abstract

The invention is suitable for the technical field of stem cell culture carrier input devices, and provides a stem cell culture carrier input device and a use method thereof, wherein the stem cell culture carrier input device comprises a frame, a culture medium bottle, an injection pump, a waste liquid bottle, a buffer bottle, a water-boiling bottle, an electric control plate, a carrier input pipe, a vertical lifter, a pipe clamping valve plate, a rotary table and an injector; according to the invention, by integrating the components such as the vertical lifter, the pipe clamping valve plate, the carrier input pipe, the rotary table, the injection pump and the like, the automatic input of the stem cell culture carrier and the automatic replacement of the culture medium are realized, the rotary table can be used for placing a plurality of carrier bottles and accurately rotating and positioning, the vertical lifter drives the pipe clamping valve plate and the carrier input pipe to move up and down, so that the needle head structure at the lower end of the carrier input pipe accurately pierces the carrier bottle cap to complete the insertion and separation, the whole process is uniformly and cooperatively controlled by the electric control board, the manual uncovering operation is not needed, and the pollution risk is effectively reduced.

Inventors

  • ZENG LINGWEN
  • ZHONG HUAQIANG
  • TAN WEIFENG
  • GUO XIAOMEI
  • MA XIAOLONG
  • ZHANG SI
  • ZHANG PENG

Assignees

  • 中科志康(广州)科学技术有限公司

Dates

Publication Date
20260505
Application Date
20260114

Claims (10)

  1. 1. A stem cell culture carrier feeding device, comprising: The device comprises a rack (12), wherein the rack (12) is provided with an injection pump (3), a buffer bottle (5), a boiled water bottle (6), an electric control plate (7), a culture medium bottle (8) and a waste liquid bottle (9); The vertical lifter (2) is vertically arranged on the frame (12), the pipe clamping valve plate is in transmission connection with the vertical lifter (2), and the carrier input pipe (10) is vertically arranged and connected with the pipe clamping valve plate; Carousel (11), the level set up in on frame (12) and be located carrier input pipe (10) below, carousel (11) are used for placing a plurality of carrier bottle (1), syringe (4) are connected with syringe pump (3) and are passed through pipeline and culture medium bottle (8), waste liquid bottle (9), buffer bottle (5), vacuum flask (6) intercommunication.
  2. 2. Stem cell culture carrier input device according to claim 1, characterized in that the pinch valve plate is provided with a plurality of pinch valves for controlling the on-off of different pipelines, and the carrier input tube (10) passes through the pinch valve plate and is controlled by the pinch valves.
  3. 3. Stem cell culture carrier feeding device according to claim 2, characterized in that the vertical lifter (2) drives the pinch valve plate to move up and down in the vertical direction, so that the lower end of the carrier feeding tube (10) is inserted into or separated from the carrier bottle (1) on the turntable (11).
  4. 4. Stem cell culture carrier feeding device according to claim 2, characterized in that the turntable (11) is provided with a plurality of carrier bottles (1) for placing carrier bottles (1), the turntable (11) being rotatable to align different carrier bottles (1) under the carrier feeding tube (10).
  5. 5. The stem cell culture carrier input device according to claim 2, wherein the injection pump (3) is selectively communicated with the culture medium bottle (8), the buffer bottle (5), the boiled water bottle (6) and the waste liquid bottle (9) through pipelines, so that liquid can be pumped or discharged.
  6. 6. The stem cell culture carrier input device according to claim 1, wherein the electric control board (7) is electrically connected with the vertical lifter (2), the tube clamping valve plate, the injection pump (3) and the rotary table (11) and is used for controlling the coordinated action of all the components.
  7. 7. The stem cell culture carrier input device according to claim 6, wherein the buffer bottle (5), the boiled water bottle (6) and the culture medium bottle (8) are respectively communicated with the injection pump (3) through independent pipelines, and pinch valves are arranged on the pipelines to realize liquid switching.
  8. 8. The stem cell culture carrier feeding device according to claim 6, wherein the lower end of the carrier feeding tube (10) is a needle structure for piercing the cap of the carrier bottle (1) and injecting or extracting the liquid into the bottle.
  9. 9. Stem cell culture carrier feeding device according to claim 1, characterized in that the syringe (4) is internally connected with a peristaltic pump for precise control of the liquid extraction and injection quantity.
  10. 10. A stem cell culture carrier input method applied to the input device of any one of claims 1 to 9, characterized by comprising the steps of: The starting device is used for controlling the turntable to rotate through the electric control board, and aligning a carrier bottle to be put into a carrier to the lower part of the carrier input pipe; S2, controlling the vertical lifter to descend so that the lower end of the carrier input pipe is inserted into the carrier bottle; S3, pumping waste liquid in the carrier bottle to a waste liquid bottle through a syringe pump and a pinch valve plate, or sequentially pumping buffer solution and boiled water to clean a carrier input pipe; S4, extracting the culture medium in the culture medium bottle, and injecting the culture medium into the carrier bottle through the carrier injection pipe; s5, putting the stem cell culture carrier into a culture device from a carrier putting tube; And S6, controlling the vertical lifter to ascend so that the carrier input pipe is separated from the carrier bottle, and repeating the steps S1 to S5 to perform the next carrier bottle operation after single input is completed.

Description

Stem cell culture carrier input device and use method Technical Field The application relates to the technical field of stem cell culture carrier input devices, in particular to a stem cell culture carrier input device and a use method thereof. Background Stem cell culture is a basic technology in fields of regenerative medicine, cell therapy, tissue engineering, etc., and with rapid development of clinical application of stem cells, there is an increasing demand for large-scale expansion culture of stem cells, and stem cell culture is generally required to be performed on specific carriers, such as microcarriers, gel beads or three-dimensional scaffolds, etc., to provide suitable adhesion and growth microenvironments, and these carriers are required to be put into carrier bottles or culture containers containing a culture medium during the culture process, and the culture medium is periodically replaced to maintain normal proliferation and differentiation of cells. However, in the above scheme, the conventional stem cell culture carrier input and culture medium replacement mainly rely on manual operation, and an operator manually completes the steps of carrier input, waste liquid extraction, pipeline cleaning, fresh culture medium injection and the like in a sterile operation table or a biological safety cabinet; secondly, the operation efficiency is low, the number of single-time treatment bottles is limited, the requirement of large-scale culture cannot be met, thirdly, the consistency of manual operation is poor, the fluctuation of culture conditions is easily caused by the difference of the methods of different operators, the repeatability of stem cell expansion among batches is affected, and fourthly, the labor intensity is high, the fatigue of operators is easily caused by long-time repeated operation, and the pollution and error risk is further increased. In the prior art, although some automatic cell culture devices, such as a full-automatic cell culture instrument or a pipetting workstation, are mainly designed aiming at a plane or a small-volume container such as a culture dish, an orifice plate or a T bottle, are difficult to adapt to a microcarrier suspension culture system taking a carrier bottle as a container, the automatic liquid treatment devices aiming at the carrier bottle are less, the existing devices usually lack special carrier input functions, or the integrated automatic operation of waste liquid extraction, pipeline cleaning, culture medium injection and carrier input cannot be realized, meanwhile, the cross contamination problem of a needle or a pipette is not solved effectively when the existing devices continuously treat a plurality of bottles, the cleaning function is imperfect or the cleaning efficiency is low, and the strict requirements of stem cell culture on sterility and operation accuracy are difficult to be satisfied. Disclosure of Invention The invention provides a stem cell culture carrier input device and a using method thereof, and aims to solve the problems in the background technology. In order to achieve the above purpose, the present application provides the following technical solutions: a stem cell culture carrier input device and a use method thereof comprise the following steps: The device comprises a rack, wherein the rack is provided with an injection pump, a buffer bottle, a boiled water bottle, an electric control plate, a culture medium bottle and a waste liquid bottle; the vertical lifter is vertically arranged on the frame, the pipe clamping valve plate is in transmission connection with the vertical lifter, and the carrier input pipe is vertically arranged and connected with the pipe clamping valve plate; the rotary table is horizontally arranged on the frame and positioned below the carrier input pipe, the rotary table is used for placing a plurality of carrier bottles, and the injector is connected with the injection pump and is communicated with the culture medium bottle, the waste liquid bottle, the buffer bottle and the boiled water bottle through pipelines. In any of the above technical solutions, further, the pinch valve plate is provided with a plurality of pinch valves for controlling on-off of different pipelines, and the carrier input pipe passes through the pinch valve plate and is controlled by the pinch valves. In any of the above technical solutions, further, the vertical lifter drives the pinch valve plate to move up and down along a vertical direction, so that the lower end of the carrier input tube is inserted into or separated from the carrier bottle on the turntable. In any of the above solutions, further, a plurality of carrier bottle positions are provided on the turntable for placing carrier bottles, and the turntable is rotatable to align different carrier bottles under the carrier input tube. In any of the above technical solutions, further, the injection pump is selectively communicated with the culture medium bottle, the bu