Search

CN-121975620-A - Immune cell culture perfusion device

CN121975620ACN 121975620 ACN121975620 ACN 121975620ACN-121975620-A

Abstract

The invention belongs to the technical field of immune cell culture perfusion, and particularly relates to an immune cell culture perfusion device which comprises a base, wherein a plurality of culture trays are arranged at the top of the base, a fixed block is inserted and installed at the bottom of each culture tray, a supporting rod is rotatably installed at the top of the base, a sliding groove is formed in each supporting rod, a lifting plate is slidably installed in each sliding groove, a pump body is fixedly installed at the top of each lifting plate, a water outlet pipe is fixedly installed at the water outlet end of each pump body, a plurality of connecting pipes penetrate through each lifting plate and are fixedly installed at the bottom ends of the plurality of connecting pipes, atomizing spray heads are fixedly installed at the water inlet ends of the pump bodies respectively positioned in the plurality of culture trays.

Inventors

  • MA HUI
  • CHEN XIAODONG
  • XU QIAN
  • LI SHUNBIN

Assignees

  • 江苏颐泽生物科技有限公司

Dates

Publication Date
20260505
Application Date
20260206

Claims (8)

  1. 1. An immune cell culture perfusion device, comprising: The device comprises a base (1), wherein a plurality of culture trays (2) are arranged at the top of the base (1), a fixed block (23) is inserted and installed at the bottom of the culture trays (2), a supporting rod (5) is rotatably installed at the top of the base (1), a sliding groove (6) is formed in the supporting rod (5), a lifting plate (7) is slidably installed in the sliding groove (6), a pump body (8) is fixedly installed at the top of the lifting plate (7), a water outlet pipe (9) is fixedly installed at the water outlet end of the pump body (8), a plurality of connecting pipes (4) penetrate through the lifting plate (7) and are fixedly installed at the bottom end of the water outlet pipe (9), atomizing spray heads (3) are fixedly installed at the bottom ends of the connecting pipes (4) and are respectively located in the culture trays (2), a water inlet pipe (13) is fixedly installed at the water inlet end of the pump body (8), a threaded rod (14) is rotatably installed in the sliding groove (6), a driving motor (15) is fixedly installed at the top of the lifting plate (7), and a driving motor (15) is fixedly installed in the supporting rod (5), and the driving motor (15) is coaxially connected with the threaded rod (14); The limiting component is positioned in the base (1) and used for limiting the supporting rod (5); The driving assembly is positioned in the base (1) and is used for driving the fixed blocks (23) to rotate; the fixing components are respectively positioned in the culture plates (2) and are respectively used for fixing the culture plates (2).
  2. 2. The immune cell culture perfusion device of claim 1, wherein the limiting assembly comprises: The movable groove (16), movable groove (16) is seted up in base (1), movable groove (16) sliding mounting has stopper (17), one end of stopper (17) runs through one side of movable groove (16) and extends to in bracing piece (5), the other end fixed mounting of stopper (17) has pull rod one (18), the opposite side of movable groove (16) is run through to one end of pull rod one (18) and extend to the external world, just be located on pull rod one (18) and be equipped with spring one (19) in movable groove (16) endotheca.
  3. 3. The immune cell culture perfusion device according to claim 2, wherein one end of the limiting block (17) is in plug-in fit with the supporting rod (5), the first pull rod (18) is in sliding connection with the moving groove (16) and the base (1), and two ends of the first spring (19) are respectively and tightly welded with the limiting block (17) and the inner wall of the moving groove (16).
  4. 4. The immune cell culture perfusion device of claim 1, wherein the drive assembly comprises: The rotary groove (20), rotary groove (20) is seted up in base (1), rotary disk (21) is installed in rotary groove (20) internal rotation, top fixed mounting of rotary disk (21) has toothed ring (11), toothed ring (11) inner gearing installs a plurality of gears (22), the top of gear (22) runs through rotary groove (20) top and with corresponding fixed block (23) fixed connection, the top and the fixed block (23) fixed connection that correspond of rotary disk (21), drive groove (27) have been seted up to the below that just is located rotary groove (20) in base (1), drive groove (27) internal rotation is installed gear two (28), the top of gear two (28) runs through the top of drive groove (27) and with rotary disk (21) coaxial coupling, one side and the being located drive groove (27) inner gearing of gear two (28) install gear three (29), the bottom fixed mounting of base (1) has drive motor (10), the bottom of one and the output of drive end (10) run through three coaxial coupling of base (29).
  5. 5. The immune cell culture perfusion device according to claim 4, wherein the toothed ring (11) is rotationally connected with the rotary groove (20), the first gear (22) is rotationally connected with the rotary groove (20), the third gear (29) is rotationally connected with the driving groove (27), and the output shaft of the first driving motor (10) is rotationally connected with the base (1).
  6. 6. The immune cell culture perfusion device of claim 1, wherein the fixation assembly includes: Spout (12), spout (12) are seted up in culture dish (2), slidable mounting has bullet piece (24) in spout (12), one end of bullet piece (24) runs through one side of spout (12) and extends to in fixed block (23), the other end fixed mounting of bullet piece (24) has pull rod two (25), the opposite side that one end of pull rod two (25) runs through spout (12) and extends to the external world, just be located on pull rod two (25) and spout (12) endotheca and be equipped with spring two (26).
  7. 7. The immune cell culture perfusion device according to claim 6, wherein one end of the elastic block (24) is in plug-in fit with the fixed block (23), the second pull rod (25) is in sliding connection with the sliding chute (12) and the culture dish (2), and two ends of the second spring (26) are respectively and tightly welded with the elastic block (24) and the inner wall of the sliding chute (12).
  8. 8. The immune cell culture perfusion device according to claim 1, wherein the threaded rod (14) is in threaded connection with the lifting plate (7), and the output shaft of the driving motor II (15) is in rotational connection with the support rod (5).

Description

Immune cell culture perfusion device Technical Field The invention belongs to the technical field of immune cell culture perfusion, and particularly relates to an immune cell culture perfusion device. Background The immune cell culture technology has an indispensable important role in the fields of biomedicine, tumor treatment, immunotherapy research and development and the like, and has the core aim of providing proper nutrition supply and growth conditions for immune cells by simulating in-vivo physiological environments, realizing proliferation, activation and function maintenance of the cells, further meeting the requirements of scenes such as clinical application, experimental study and the like, and being widely applied to the immune cell culture process by taking perfusion culture as a key mode of large-scale and high-quality culture of the immune cells and taking the advantages of sustainable nutrient supplement and timely removal of metabolic wastes. The existing immune cell culture perfusion device is used for conveying culture mediums into a culture dish through a spray head, but the problem that the culture mediums are unevenly distributed is commonly caused, the culture mediums sprayed out of the traditional spray head are prone to local aggregation and insufficient in adhesion, so that the contact area between the culture mediums and the inner wall of the culture dish is limited, immune cells in the culture dish cannot uniformly contact with nutrient substances, in addition, the culture dish of the existing immune cell culture perfusion device is designed to be fixedly connected or a complex disassembly structure, after immune cell culture is completed, an operator needs to complete disassembly of the culture dish through multiple steps of complex operation by means of special tools, time and labor are consumed, experimental operation efficiency is reduced, mechanical damage is possibly caused to the immune cells after the culture is completed in the disassembly process, cell activity is affected, meanwhile, the complex disassembly structure also brings inconvenience to subsequent cleaning and disinfection work of the culture dish, residual cell debris and culture medium impurities are prone to bacteria, pollution risks in the subsequent culture process are increased, and the connection structure is prone to abrasion is repeatedly disassembled, so that the whole tightness and the service life of the device are affected. Disclosure of Invention The present invention is directed to an immune cell culture perfusion device, which solves the problems set forth in the background art. In view of this, the present invention provides an immune cell culture perfusion device, comprising: The device comprises a base, wherein a plurality of culture trays are arranged at the top of the base, a fixed block is inserted and installed at the bottom of the culture trays, a supporting rod is rotatably installed at the top of the base, a sliding groove is formed in the supporting rod, a lifting plate is slidably installed in the sliding groove, a pump body is fixedly installed at the top of the lifting plate, a water outlet pipe is fixedly installed at the water outlet end of the pump body, a plurality of connecting pipes penetrate through the lifting plate and are fixedly installed at the bottom end of the water outlet pipe, atomizing nozzles are fixedly installed at the bottom ends of the connecting pipes and are respectively positioned in the plurality of culture trays, a water inlet pipe is fixedly installed at the water inlet end of the pump body, a threaded rod is rotatably installed in the sliding groove, one end of the threaded rod penetrates through the lifting plate, a driving motor II is fixedly installed in the supporting rod, and the output end of the driving motor II is coaxially connected with the threaded rod; The limiting component is positioned in the base and used for limiting the supporting rod; The driving assembly is positioned in the base and used for driving the fixed blocks to rotate; The fixing components are respectively positioned in the culture plates and are respectively used for fixing the culture plates. In the technical scheme, when the culture medium storage device is used, a person can drive a plurality of fixed blocks to rotate through the driving assembly, the plurality of fixed blocks can drive a plurality of culture plates to rotate, then, the person can start the pump body, fresh culture medium can be conveyed into the water outlet pipe through the water inlet pipe on the pump body, the culture medium in the water outlet pipe can be distributed into a plurality of connecting pipes, and finally, the culture medium is atomized and sprayed into a plurality of culture plates through a plurality of atomizing nozzles, and as the culture plates rotate, the culture medium sprayed by the atomizing nozzles can be uniformly adhered to the inner walls of the culture plates, the surface