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CN-121975621-A - Cell positive-negative pressure and mechanical stimulation culture device

CN121975621ACN 121975621 ACN121975621 ACN 121975621ACN-121975621-A

Abstract

The invention relates to the technical field of biomedical engineering and cell mechanics stimulation, in particular to a cell positive-negative pressure and mechanics stimulation culture device which comprises a shell, a storage battery, a controller, a placement component, an adjusting component and a connecting plate, wherein four corners of the bottom of the shell are fixedly connected with supporting feet, the storage battery is fixedly arranged in the shell, the controller is fixedly connected in the shell, the placement component is arranged in the shell in a sliding manner, the adjusting component is arranged at the upper end in the shell, the connecting plate is connected at the middle end in the shell in a sliding manner, the side wall of the connecting plate is fixedly connected with the moving end of an electric push rod, and the electric push rod is fixedly connected at the inner top of the shell. According to the invention, the negative pressure box and the pressurizing box are respectively connected through the negative pressure pump and the pressurizing pump, and the negative pressure environment and the positive pressure environment of the culture chamber in the culture dish can be quickly and independently regulated by matching with the accurate control of the first electromagnetic valve and the second electromagnetic valve, and the pressure sensor in the test hole collects data in real time, so that closed-loop control is formed, the stability of pressure parameters is ensured, and the mechanical stress born by cells in vivo is simulated.

Inventors

  • LIU NI
  • MEI XIAOPENG
  • BU GANG
  • SUN HONGRONG
  • LIU MIAN

Assignees

  • 西安交通大学医学院第一附属医院
  • 上海埃斯埃医疗技术有限公司

Dates

Publication Date
20260505
Application Date
20260207

Claims (10)

  1. 1. The cell positive-negative pressure and mechanical stimulation culture device comprises a shell (1), and four corners of the bottom of the shell are fixedly connected with supporting feet (2); A storage battery (4) fixedly arranged in the housing (1); the controller (5) is fixedly connected in the shell (1); the device is characterized by comprising a placement component (3) which is arranged in the shell (1) in a sliding way; an adjusting component (6) arranged at the upper end in the shell (1); The connecting plate (7) is connected to the inner middle end of the shell (1) in a sliding way, the side wall of the connecting plate (7) is fixedly connected with the moving end of an electric push rod, and the electric push rod is fixedly connected to the inner top of the shell (1); The adjusting cavity (8) is fixedly arranged at the bottom of the connecting plate (7), and a rubber plug (9) is detachably connected to the bottom of the adjusting cavity (8); the top of the adjusting cavity (8) is respectively connected with a testing hole (10), a first suction pipe (11) and a second suction pipe (12), and a pressure sensor is arranged in the testing hole (10) and used for collecting positive and negative pressure signals in the adjusting cavity (8) in real time; The negative pressure box (13) is fixedly connected to the inner side wall of the shell (1); the pressurizing box (15) is fixedly connected to the inner side wall of the shell (1); The upper end of the first suction pipe (11) penetrates into the negative pressure box (13), and the first suction pipe (11) is positioned in the top area of the negative pressure box (13) and is connected with one end of the first electromagnetic valve (14); the upper end of the second suction pipe (12) penetrates into the pressurizing box (15), and the second suction pipe (12) is positioned in the top area of the pressurizing box (15) and is connected with one end of the second electromagnetic valve (16); The negative pressure pump (17) is fixedly connected to the side wall of the shell (1), and one end of the negative pressure pump (17) is communicated with the negative pressure box (13) through a conveying pipe; The booster pump (18) is fixedly connected to the side wall of the shell (1), and one end of the booster pump (18) is communicated with the inside of the booster box (15) through a conveying pipe.
  2. 2. The device for cultivating positive and negative pressure and mechanical stimulation of cells according to claim 1, wherein the placement component (3) comprises a placement frame (31), the placement frame (31) is inserted into the shell (1) in a sliding manner, a culture dish (32) is placed on the placement frame (31), a linear driver is fixedly connected to the inner bottom of the placement frame (31), the linear driver is connected with an adjusting plate (33), the top of the adjusting plate (33) is fixedly connected with a driving motor (35), an output shaft of the driving motor (35) is fixedly connected with the center of a circle of a disc (34), and a coil is arranged in the disc (34).
  3. 3. The positive and negative cell pressure and mechanical stimulation culture device according to claim 2, wherein a light supplementing lamp (36) and a shooting device (37) are fixedly connected to the top of the disc (34).
  4. 4. The device for cultivating positive and negative cell pressure and mechanical stimulation according to any one of claims 1-3, wherein the adjusting component (6) comprises a first gear ring (61), a negative pressure control box is fixedly connected to the inner side wall of the shell (1), the bottom of the negative pressure control box is rotatably connected with the first gear ring (61), and a first connecting motor (62) is connected to the bottom of the negative pressure control box through bolts.
  5. 5. The device for culturing positive and negative cell pressure and mechanical stimulation according to claim 4, wherein the output shaft of the first connecting motor (62) drives the first driving gear (63) to be meshed with the first gear ring (61), and the first gear ring (61) is fixedly connected with the connecting disc (64).
  6. 6. The positive and negative cell pressure and mechanical stimulation culture device according to claim 5, wherein the connection disc (64) is connected with a second connection motor (65) through bolts, and an output shaft of the second connection motor (65) is fixedly connected with a second driving gear (66).
  7. 7. The device for cultivating positive and negative pressure and mechanical stimulation of cells according to claim 6, wherein the second driving gear (66) is meshed to drive the second gear ring (67) to be rotationally connected with the connecting disc (64), and a plurality of groups of arc-shaped grooves (68) are embedded in the second gear ring (67).
  8. 8. The positive-negative pressure and mechanical stimulation cell culture device according to any one of claims 4 to 7, characterized in that a plurality of groups of driving grooves (69) are embedded in the connecting disc (64), and the driving grooves (69) are connected with a driving plate (610) in a sliding manner; the other end of the driving plate (610) is fixedly connected with a protruding rod (611), and the protruding rod (611) penetrates through the arc-shaped groove (68) outwards in a sliding manner and is fixedly connected with the cross rod (612); The other end of the cross rod (612) is magnetically attracted and connected with the side wall of the upper end of the accommodating frame (613) through a magnet, and an opening is formed in the bottom of the accommodating frame (613) and connected with the electromagnetic valve (614).
  9. 9. The positive and negative cell pressure and mechanical stimulation culture device according to claim 8, wherein a group of the protruding rods (611) are fixedly connected with a linear driver (615); The movable end of the linear driver (615) is fixedly connected with the suction pipe (616), the bottom of the suction pipe (616) is connected with the suction disc (617), and the upper end of the suction pipe (616) is connected with the negative pressure control box.
  10. 10. The positive-negative pressure and mechanical stimulation cell culture device according to claim 8, wherein a plurality of groups of cross bars (612), accommodating frames (613) and electromagnetic valves (614) are arranged, and the cross bars are arranged at four corners of the second gear ring (67) at equal angles.

Description

Cell positive-negative pressure and mechanical stimulation culture device Technical Field The invention belongs to the technical field of biomedical engineering and cell mechanics stimulation, and particularly relates to a positive and negative pressure bidirectional pressure loading device for in-vitro cell or tissue culture, which can realize controllable positive pressure and negative pressure stimulation on contents. Background In the research of tissue engineering and regenerative medicine, mechanical stimulation plays an important role in regulating and controlling cell behaviors, differentiation and functions, and in-vitro simulation of in-vivo mechanical environment is a key link for researching cell response mechanisms and developing clinical treatment strategies. Pressure stimulation is an important mechanical factor, and precise control of its mode of action (positive/negative pressure) and parameters (amplitude, frequency, duration) is critical to reproducibility and physiological relevance of cellular experiments. The organoids can be used as three-dimensional micro-organ models to better simulate the structure and function of human organs, but in-vitro culture still faces the bottlenecks of incomplete microenvironment simulation, insufficient functional maturity and the like. Mechanical stimulation, particularly multidirectional pressure loading, is an important technical means for reconstructing the development microenvironment in organoids. In the prior art, in-vitro pressure loading has the following limitations that 1, the existing equipment generally only supports static positive pressure or negative pressure stimulation in a single direction, bidirectional pressure load cannot be applied simultaneously or alternately, so that complex mechanical states of internal tissues in the processes of breathing, peristaltic motion, blood flow pulsation and the like are difficult to reproduce, 2, most devices can only apply pressure stimulation to one group of culture samples at one time, multi-channel or parallel operation capability is lacking, intervention cannot be applied synchronously to an experiment group and a control group under the same experiment condition, efficiency and comparability of results of the experiment are influenced, 3, monitoring function is lacking, the state of culture contents cannot be monitored in real time when pressure intervention is applied, association of pressure stimulation and cell/organoid response is difficult to be captured dynamically, 4, the prior art can only provide a constant pressure or simple pressure change mode, and cannot realize a complicated mechanical mode with physiological relevance such as periodical alternating pressure, pulse pressure or pressure fluctuation and the like, application of the organoid development simulation, disease model construction and the like is limited, 5, the whole performance of the devices is uneven, and the requirements of short plates and the experiment precision and the intelligent degree are difficult to meet. The existing external pressure loading device cannot fully meet the requirements of researches on complex and physiological related mechanical stimulation environments, and development of a novel pressure loading system capable of realizing bidirectional pressure controllable loading, diversified pressure modes and having real-time monitoring capability is needed. Disclosure of Invention The invention provides a technical scheme for achieving the purpose, which comprises a shell, a storage battery, a controller, a placement component, an adjusting component, a connecting plate, an adjusting cavity, a test hole, a first suction pipe and a second suction pipe, wherein four corners of the bottom of the shell are fixedly connected with supporting feet, the storage battery is fixedly arranged in the shell, the controller is fixedly connected in the shell, the placement component is arranged in the shell in a sliding mode, the adjusting component is arranged at the upper end in the shell, the connecting plate is fixedly connected with the moving end of an electric push rod, the electric push rod is fixedly connected to the inner top of the shell, the adjusting cavity is fixedly arranged at the bottom of the connecting plate, the bottom of the adjusting cavity is detachably connected with a rubber plug, the top of the adjusting cavity is respectively connected with a test hole, the first suction pipe and the second suction pipe, a pressure sensor is arranged in the test hole and used for collecting positive and negative pressure signals in the adjusting cavity in real time, the negative pressure box is fixedly connected to the inner side wall of the shell, the first electromagnetic valve penetrates into the negative pressure box, the upper end of the first suction pipe is located in the negative pressure box, the top area of the negative pressure box is connected with one end of the first electromagnetic valve, th