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CN-121975622-A - Cell encapsulation oxygen production device and application thereof

CN121975622ACN 121975622 ACN121975622 ACN 121975622ACN-121975622-A

Abstract

The invention provides a cell packaging oxygen generating device and application thereof, belonging to the technical field of implantable medical devices, wherein the cell packaging oxygen generating device comprises a raw material storage module, a storage module and a control module, wherein the raw material storage module is used for storing hydrogen peroxide solution; the diffusion control module is arranged between the raw material storage module and the cell packaging module and comprises a catalytic cavity and an isolation structure, a catalyst is arranged in the catalytic cavity and is used for catalyzing hydrogen peroxide molecules to generate oxygen, the isolation structure comprises a first isolation film and a second isolation film, the first isolation film is arranged between the raw material storage module and the catalytic cavity and is used for controlling the diffusion of the hydrogen peroxide molecules into the catalytic cavity, the second isolation film is arranged between the catalytic cavity and the cell packaging module and is used for controlling the diffusion of the oxygen into the cell packaging module, and the cell packaging module is used for accommodating cell packaging matrixes containing cells and is used for receiving the oxygen and supplying oxygen for the cells. The cell packaging oxygen generating device can control the oxygen generating rate and match the oxygen consumption requirement of cells, effectively avoids the toxicity of hydrogen peroxide and the migration risk of a catalyst, and has excellent biological safety.

Inventors

  • WANG LONGHAI
  • CHEN MINGYU

Assignees

  • 中国科学技术大学

Dates

Publication Date
20260505
Application Date
20260403

Claims (10)

  1. 1. The cell packaging oxygen generating device is characterized by comprising a raw material storage module, a diffusion control module and a cell packaging module, wherein, The raw material storage module is used for storing hydrogen peroxide solution; The diffusion control module is arranged between the raw material storage module and the cell packaging module and comprises a catalytic cavity and an isolation structure, wherein, The catalytic cavity is suitable for receiving hydrogen peroxide molecules which are diffused and conveyed by the raw material storage module, and a catalyst is arranged in the catalytic cavity and is used for catalyzing the hydrogen peroxide molecules entering the catalytic cavity to generate oxygen; The isolation structure comprises a first isolation film and a second isolation film, wherein the first isolation film is arranged between the raw material storage module and the catalytic cavity and is used for controlling hydrogen peroxide molecules to enter the catalytic cavity in a diffusion manner; the cell encapsulation module is used for receiving the oxygen which is diffused and transferred by the diffusion control module and supplying oxygen to cells in the cell encapsulation matrix.
  2. 2. The cell encapsulation oxygen generating apparatus of claim 1, wherein the oxygen generating apparatus comprises a plurality of oxygen generating cells, The thickness of the first isolating film is 0.1-2 mm, and the surface area is 50-300 mm 2 ; the thickness of the second isolating film is 0.05-2 mm, and the surface area is 80-400 mm 2 ; The materials of the first isolation film and the second isolation film are independently selected from at least one of polydimethylsiloxane, fluorosilicone rubber, polyurethane, thermoplastic polyurethane, polyether block amide, polyethylene, polypropylene, polytetrafluoroethylene, expanded polytetrafluoroethylene, fluorinated ethylene propylene, perfluoroalkoxyalkane, ethylene-tetrafluoroethylene copolymer, polyvinylidene fluoride, polyether sulfone, polysulfone, nylon, polycarbonate, polyacrylonitrile and cellulose.
  3. 3. The cell encapsulation oxygen generating apparatus of claim 1, wherein the oxygen generating apparatus comprises a plurality of oxygen generating cells, The initial concentration of the hydrogen peroxide solution is 0.1 wt% -30 wt%.
  4. 4. The cell encapsulation oxygen generating apparatus of claim 1, wherein the oxygen generating apparatus comprises a plurality of oxygen generating cells, The thickness of the catalytic cavity is 0.1-2 mm, the volume is 10-800 mm 3 , The catalyst is arranged in the catalytic cavity in any one form of powder filling, particle filling, porous block filling and immobilized coating, and the filling amount is 0.1-5 g/cm 3 ; the catalyst comprises at least one of an inorganic catalyst, a noble metal supported catalyst and an enzyme catalyst.
  5. 5. The cell encapsulation oxygen generating apparatus of claim 1, wherein the oxygen generating apparatus comprises a plurality of oxygen generating cells, The cell packaging matrix is hydrogel packaged with cells; The hydrogel comprises at least one of alginate hydrogel, agarose hydrogel, polyethylene glycol diacrylate hydrogel, polyethylene glycol hydrogel, methacryloylated gelatin, hyaluronic acid hydrogel, fibrin hydrogel, chitosan hydrogel, dextran hydrogel, polyvinyl alcohol hydrogel and pluronic hydrogel.
  6. 6. The cell encapsulation oxygen generating apparatus of claim 1, wherein the oxygen generating apparatus comprises a plurality of oxygen generating cells, The cell encapsulation module comprises a groove encapsulation cavity for accommodating the cell encapsulation matrix; the depth of the groove type packaging cavity is 0.5-10 mm, and the effective volume is 50-2000 mu L.
  7. 7. The cell encapsulation oxygen generating apparatus of claim 6, wherein the oxygen generating apparatus comprises a plurality of oxygen generating cells, An opening is formed in one end of the groove type packaging cavity, and a sealing film is further arranged at the opening and used for sealing the groove type packaging cavity; The sealing membrane is a semi-permeable membrane, the aperture is 50-500 nm, and the thickness is 10-200 mu m; The semipermeable membrane is made of at least one of polytetrafluoroethylene, expanded polytetrafluoroethylene, polyethersulfone, polyvinylidene fluoride, polyacrylonitrile, polycarbonate, nylon, cellulose and polyurethane.
  8. 8. The cell-encapsulated oxygen-generating apparatus of claim 1, further comprising at least one of a gas buffer structure, a liquid-feeding replenishment interface, and a residual raw material removal layer, wherein, The gas buffer structure is arranged between the second isolating membrane and the cell packaging module or embedded in the second isolating membrane and is used for buffering gas pressure fluctuation in the process of generating oxygen by the hydrogen peroxide molecules; The liquid adding and replenishing interface is arranged on the raw material storage module and is configured as a disposable sealing interface or a repeatedly openable and closable replenishing interface and is used for filling or replenishing hydrogen peroxide solution into the raw material storage module; the residual raw material removing layer is arranged between the second isolating film and the cell packaging module and is used for capturing and decomposing unreacted hydrogen peroxide molecules.
  9. 9. The cell encapsulation oxygen generating apparatus of claim 1, wherein the oxygen generating apparatus comprises a plurality of oxygen generating cells, The shape of the cell encapsulation oxygen generating device comprises any one of a sheet-shaped structure, a disc-shaped structure or a strip-shaped structure; the height of the cell packaging oxygen generating device is 2-20 mm, and the transverse dimension is 10-60 mm.
  10. 10. An implantable medical device, comprising the cell encapsulation oxygen generating apparatus of any one of claims 1 to 9.

Description

Cell encapsulation oxygen production device and application thereof Technical Field At least one embodiment of the invention relates to the technical field of implantable medical devices, in particular to a cell encapsulation oxygen generating device and application thereof. Background Cell transplantation treatment is a treatment strategy with clinical prospect aiming at diseases such as type 1 diabetes, and in clinical application, transplanted cells are usually isolated from a host immune system through a packaging structure, so that the risk of immune rejection is reduced, and the long-term use of immunosuppressive drugs is reduced. However, the packaging structure inevitably increases the transmission resistance of substances such as oxygen and the like while realizing immune isolation. The solubility of oxygen in the tissue in vivo is low, the effective diffusion distance is limited, and in the early stage of cell transplantation, persistent hypoxia easily occurs in the cavity of the packaging structure, so that the activity of transplanted cells is reduced, the functions are attenuated and even necrotized, and the long-term curative effect of packaged cell therapy is limited. To alleviate the early hypoxia problem of transplanted cells, attempts have been made to enhance local oxygen supply using a variety of oxygen delivery strategies, including, but not limited to, methods using highly oxygen permeable materials with thin film structures, exogenous oxygen supplying or carrying materials, integrating oxygen generating modules in packaging systems, and the like. However, the above methods generally have the problems of insufficient controllability, complex device structure, frequent oxygen source supplement required for part of the methods, difficulty in stably maintaining oxygen supply flux matched with cell oxygen consumption for a long time, and the like. Disclosure of Invention Accordingly, it is an objective of the present invention to provide a cell-encapsulated oxygen generating device and application thereof, which at least partially solve at least one of the above-mentioned problems. In one aspect of the invention, a cell-encapsulated oxygen generating apparatus is provided that includes a raw material storage module, a diffusion control module, and a cell encapsulation module. And the raw material storage module is used for storing the hydrogen peroxide solution. The diffusion control module is arranged between the raw material storage module and the cell packaging module and comprises a catalytic cavity and an isolation structure. A catalytic cavity adapted to receive the hydrogen peroxide solution diffusion-transported from the raw material storage module; the inside of the catalytic cavity is provided with a catalyst which is used for catalyzing hydrogen peroxide molecules entering the catalytic cavity to generate oxygen. The isolation structure comprises a first isolation film and a second isolation film, wherein the first isolation film is arranged between the raw material storage module and the catalytic cavity and is used for controlling hydrogen peroxide molecules to enter the catalytic cavity in a diffusion mode, and the second isolation film is arranged between the catalytic cavity and the cell packaging module and is used for controlling generated oxygen to enter the cell packaging module in a diffusion mode. And the cell encapsulation module is used for receiving the oxygen which is diffused and transferred by the diffusion control module and supplying oxygen to cells in the cell encapsulation matrix. In another aspect of the invention, an implantable medical device is provided that includes the cell-encapsulated oxygen-generating device described above. According to the cell packaging oxygen generating device, the hydrogen peroxide solution is used as an oxygen supply raw material, and the diffusion flux of the hydrogen peroxide solution is controlled to enter the catalytic cavity through the first isolating membrane, so that the oxygen generating rate is regulated and controlled by the diffusion parameters, the oxygen consumption requirement of a cell packaging matrix can be matched, and the continuous adaptation of oxygen supply of cells is ensured. The catalyst in the catalytic cavity catalyzes and decomposes hydrogen peroxide molecules, forms cooperations with physical barriers of the first isolation film and the second isolation film, enables the hydrogen peroxide molecules to be converted into oxygen more efficiently in a diffusion path, reduces residual leakage probability of hydrogen peroxide, avoids catalyst migration risk, blocks direct contact of hydrogen peroxide and cells from a structural layer, and improves biosafety of the cell packaging oxygen generating device. The cell packaging module can stably contain a cell packaging matrix, receive and transmit oxygen, ensure uniform oxygen supply of cells in the matrix, and relieve the problem of cell activity reduction