CN-121975624-A - High-protein-yield euglena gracilis mutant strain and application thereof

Abstract

The invention discloses a euglena gracilis mutant strain with high protein yield and application thereof. According to the invention, the ARTP mutation breeding technology is combined with the chemical stress agent to carry out mutagenesis and screening on the euglena gracilis, and the result shows that under the condition that pyruvic acid is respectively combined with chlorpromazine hydrochloride, the lethality is stable at 93% -94%, forward mutant strains with obvious high protein properties and stable physiological states can be enriched more efficiently, and under the condition, mutant strains with obviously improved protein content and stable inheritance are directionally bred, wherein the protein content of the euglena gracilis mutant strain E3 is up to 70%, the protein content of the mutant strain E is improved by 43% compared with that of an original strain, the mutant strain has stable properties in subculture, and the microorganism preservation number is CGMCC No.46624. The euglena gracilis mutant strain with high protein yield has application prospect in the aspects of preparing high protein feed, preparing high protein functional health-care food or high protein cosmetics and the like as a feed additive.

Inventors

• MA LU
• BU DENGPAN
• ZHANG YUTONG
• ZHAN TENGFEI

Assignees

• 中国农业科学院北京畜牧兽医研究所

Dates

Publication Date

20260505

Application Date

20251229

Claims (4)

1. 1. A mutant strain of Euglena gracilis (Euglena gracilis) for producing protein, which is characterized in that the microorganism preservation number is CGMCC No. 46624.
2. 2. Use of the euglena gracilis mutant strain of claim 1 as a feed additive in the preparation of animal feed.
3. 3. Use of the euglena gracilis mutant strain of claim 1 in the preparation of high protein food.
4. 4. Use of the euglena gracilis mutant strain of claim 1 in the preparation of high protein cosmetics.

Description

High-protein-yield euglena gracilis mutant strain and application thereof Technical Field The invention relates to a microalgae mutant and application thereof, in particular to a euglena mutant with high protein yield and application thereof in the aspects of protein production, high protein feed preparation, high protein health-care food or high protein cosmetics and the like, and belongs to the field of euglena mutant and application thereof. Background Euglena is a single-cell eukaryotic organism between animals and plants, and is called euglena in botany because euglena has no cell wall, and is an ideal platform for producing high-quality proteins, unsaturated fatty acids and antioxidant substances. Common Euglena (Euglena gracilis), euglena viridis (Euglena viridis), euglena hemsleyana (Euglena sanguinea), euglena mid- (Euglenaintermedia), euglena aculeata (Euglena gasterosteus) and the like, wherein Euglena gracilis has the characteristics of various nutrition modes, fast growth and abundant intracellular nutrients, has been widely studied and is the only species in Euglena for realizing large-scale culture and industrialization. Euglena gracilis (Euglena gracilis) is a single-cell eukaryotic organism that is intermediate between animals and plants, and is widely distributed in nature. The euglena gracilis has the characteristics of animal and plant cells, can carry out photoautotrophic, mixotrophic and heterotrophic culture, and contains rich nutrient substances. In recent years, with the intensive research on biological characteristics and intracellular components of Euglena gracilis, the Euglena gracilis has important application value in medicine, food, cosmetics and feed, has very great effects in improving immunity, resisting inflammation, reducing uric acid and the like, and particularly has wide application prospect in the field of biological medicine of Euglena gracilis-specific Euglena gracilis polysaccharide and the like (Zhang Kaikai and the like. Euglena gracilis culture and research and hope of product functions thereof. Bioengineering journal of Mar.25, 2024, 40 (3): 705-721). However, the protein content of natural euglena gracilis strain is low, and it is difficult to fully meet the requirements of commercial production on raw material cost and efficiency. Therefore, there is a need for genetic improvement of euglena gracilis strains to directionally enhance their protein synthesis capacity or protein content by modern biological breeding and screening techniques. Several studies have shown that physical mutagenesis in combination with stress screening is an effective strategy for obtaining high yielding mutant algal strains. The atmospheric pressure room temperature plasma (ARTP) mutagenesis technology has been widely applied to the field of microorganism breeding because of the advantages of high mutation rate, safe operation, wide mutation spectrum and the like, and has important application prospect in the aspects of producing protein, preparing high protein feed and the like if the wild type euglena gracilis strain is subjected to mutagenesis treatment by the ARTP mutagenesis technology and is directionally selected by adding a stress agent. Disclosure of Invention The invention aims at providing a euglena gracilis mutant strain with high protein yield. The second object of the present invention is to provide a microbial preparation prepared by using the high-protein-producing euglena gracilis mutant strain. The third purpose of the invention is to apply the high-protein-yield euglena gracilis mutant strain or the microbial preparation prepared by the euglena gracilis mutant strain to the aspects of producing protein, preparing high-protein feed, preparing high-protein health-care food or high-protein cosmetics and the like. In order to solve the problems that the protein content of the natural euglena gracilis strain is low and the requirements of commercial production on the cost and efficiency of raw materials are difficult to fully meet, the invention adopts an ARTP mutagenesis technology to carry out mutagenesis treatment on the wild euglena gracilis strain, and finally obtains the euglena gracilis mutant strain with obviously improved protein content through directional breeding by adding a stress agent, thereby completing the invention. The invention provides a Euglena gracilis mutant strain E3 with high-yield protein, the microorganism preservation number is CGMCC No.46624, the classification name is Euglena gracilis, the preservation time is 2025 years 10 months 28 days, the preservation unit is China general microbiological culture Collection center, and the preservation address is North Star Xilai 1 institute of China academy of sciences 3 in the Beijing Kogyo. The invention adopts ARTP mutation breeding technology and combines chemical stress agent (pyruvic acid, chlorpromazine hydrochloride) to carry out mutation and screening on euglena gracilis so as to breed high-