CN-121975625-A - Chlorella pyrenoidosa CV-5, probiotics microcapsule and application thereof in preparing products for improving calf intestinal health

Abstract

The invention belongs to the technical field of biological products for animals, and discloses a chlorella pyrenoidosa CV-5 and probiotics microcapsule and application thereof in preparing a product for improving calf intestinal health. The preservation number of the chlorella pyrenoidosa CV-5 is CGMCC No.15206, and the Latin name is Chlorella pyrenoidosa. Compared with other algae, the chlorella pyrenoidosa CV-5 provided by the invention has remarkable advantages in improving calf intestinal health. Based on the method, the invention further develops the probiotic microcapsule which can obviously improve the survival rate of probiotics in calf abomasum environment and simultaneously reduce the abundance of pathogenic bacteria in intestinal tracts of diarrhea calves by taking the chlorella pyrenoidosa CV-5 powder and pectin as wall materials and taking the dominant probiotic bacterial strain, sodium butyrate, trypsin and specific prebiotics as core materials. The invention is suitable for livestock breeding industry, and has good application prospect in the field of improving calf intestinal health.

Inventors

• XUE YULING
• WANG SHIJIE
• LIU ZHUO
• GUO BENHENG
• LI MIAO
• FENG LILI
• HOU XINFENG
• LI MENGLU

Assignees

• 君乐宝乳业集团股份有限公司

Dates

Publication Date

20260505

Application Date

20251231

Claims (10)

1. 1. The chlorella pyrenoidosa CV-5 is characterized in that the preservation number is CGMCC No.15206 and the Latin name is Chlorella pyrenoidosa.
2. 2. A probiotic microcapsule is characterized in that the wall material of the probiotic microcapsule comprises the chlorella pyrenoidosa CV-5 of claim 1.
3. 3. The probiotic microcapsule of claim 2, wherein the wall material comprises Chlorella pyrenoidosa CV-5 powder, natural polysaccharide substances and enzyme stabilizers, wherein the preparation method of the Chlorella pyrenoidosa CV-5 powder comprises breaking wall, freeze drying and pulverizing the Chlorella pyrenoidosa CV-5 to obtain the Chlorella pyrenoidosa CV-5 powder, and/or The core material comprises probiotic composite bacteria powder, short-chain fatty acid probiotic nutrition enhancer, probiotic nutrition metabolism auxiliary enzyme and prebiotics.
4. 4. A probiotic microcapsule according to claim 3, characterized in that said natural polysaccharide substance comprises pectin, and/or The enzyme stabilizer comprises glucose oxidase, and/or The probiotic composite bacterial powder comprises at least one of bifidobacterium animalis subspecies i797 or lactobacillus paracasei N1115 and/or The short chain fatty acid probiotic nutrition enhancer comprises sodium butyrate and/or The probiotic nutrition metabolism auxiliary enzyme comprises trypsin, and/or The prebiotic comprises at least one of arabinogalactan or xylooligosaccharide.
5. 5. The probiotic micro-capsule of claim 4, wherein the total viable count of the probiotic composite bacterial powder is 10 7 CFU/g-10 10 CFU/g, and/or The final concentration of the glucose oxidase in the probiotic microcapsule is 250U/g-400U/g, and/or The prebiotics comprise arabinogalactan and xylooligosaccharide with the mass ratio of 5-10:3-8.
6. 6. The probiotic micro-capsule according to claim 3 to 5, wherein the mass ratio of the probiotic composite bacterial powder, the short-chain fatty acid-based probiotic nutrition enhancer, the probiotic nutrition metabolism auxiliary enzyme and the prebiotic is 10-20:10-15:1-3:8-18, and/or The mass ratio of the chlorella pyrenoidosa CV-5 powder to the natural polysaccharide is 3-6:1.5-3.
7. 7. The method for preparing the probiotic micro-capsules according to any one of claims 3 to 6, characterized in that the method comprises the following steps: s1, preparing core material suspension and wall material solution According to the design proportion, uniformly mixing the probiotic composite bacteria powder, the short-chain fatty acid probiotic nutrition enhancer, the probiotic nutrition metabolism auxiliary enzyme and the prebiotics, adding a protective solution, and homogenizing in an environment of 2-6 ℃ to obtain a core material suspension; Adding an enzyme stabilizer into a mixed solution with the final mass concentration of the chlorella pyrenoidosa CV-5 powder of 3-6% and the final mass concentration of the natural polysaccharide substance of 1.5-3%, and uniformly dispersing to obtain a wall material solution; S2, forming and curing the microcapsules And adding the core material suspension into the wall material solution, mixing, dripping into the calcium ion solution, solidifying, drying, and benefiting from the probiotic microcapsules.
8. 8. The method of claim 7, wherein the mass ratio of the core suspension to the wall material solution is 0.8-1.2:2.5-3.5, and/or The curing time is 20min-30min.
9. 9. Use of the chlorella pyrenoidosa CV-5 of claim 1 or the probiotic microcapsule of any one of claims 2-6 in the preparation of a product for improving calf intestinal health.
10. 10. The use according to claim 9, wherein the improvement of calf intestinal health comprises the prevention or treatment of calf diarrhea, and/or The product comprises at least one of a veterinary drug, a feed or a feed additive.

Description

Chlorella pyrenoidosa CV-5, probiotics microcapsule and application thereof in preparing products for improving calf intestinal health Technical Field The invention belongs to the technical field of biological products for animals, and particularly relates to chlorella pyrenoidosa CV-5 and application thereof. Background The calf stage is a key initial link in the industry chain of cow and beef cattle cultivation, and the intestinal health of the calf directly determines the survival rate, the growth and development speed and the later production performance of the calf. Among them, calf diarrhea is a core problem commonly existing in global scale livestock breeding, and is a primary cause of death and economic loss of newborn calves. According to statistics, the incidence rate of diarrhea of newborn calves is up to 30% -50%, the death rate of severe diarrhea calves can be up to 15% -20%, even if diarrhea calves are cured and survived, the growth and development of the diarrhea calves can be obviously inhibited, the problems of slow weight increase, delay of weaning time, reduced feed conversion rate and the like occur, and meanwhile, the production indexes of milk yield, meat yield and the like after adult are reduced, so that heavy economic burden is brought to the breeding industry. For a long time, for the treatment and prevention of calf diarrhea, the breeding industry generally depends on antibiotic drugs including sulfonamides, tetracyclines, cephalosporins and the like. Although antibiotics can inhibit or kill part of pathogenic microorganisms in a short period and relieve diarrhea symptoms, unreasonable use in a long period can cause a series of serious problems, such as induction of drug-resistant strains, disruption of calf intestinal flora balance, drug residue, inhibition of calf autoimmune development and the like. In order to solve various problems caused by the abuse of antibiotics, a green and safe alternative scheme becomes a research hot spot in the field of livestock breeding, wherein a probiotic preparation is considered as one of the most potential antibiotic alternative products because of natural advantages of adjusting intestinal flora, enhancing intestinal barrier function, improving body immunity and the like. However, when the probiotics are directly applied to prevention and control of calf diarrhea, the probiotics are difficult to stably colonize in the calf gastrointestinal tract due to extreme environmental stress of the calf gastrointestinal tract, competitive exclusion of calf intestinal flora, insufficient stability of the probiotics preparation and the like, so that the functions of the probiotics cannot be fully exerted. Disclosure of Invention In view of the above problems in the prior art, the present invention provides a Chlorella pyrenoidosa CV-5 and uses thereof. The invention creatively discovers that the chlorella pyrenoidosa CV-5 has remarkable advantages in improving the intestinal health of calves. Specifically, compared with other algae, the chlorella pyrenoidosa CV-5 provided by the invention can more effectively improve the survival rate of probiotics in calf gastric juice; in addition, compared with the combination of other algae and probiotics, the combination of the chlorella pyrenoidosa CV-5 and the probiotics can remarkably inhibit the growth and reproduction of pathogenic bacteria in intestinal tracts of diarrhea calves. Furthermore, the probiotics microcapsule developed by taking the chlorella pyrenoidosa CV-5 as the raw material in the wall material can improve the survival rate of probiotics in calf gastrointestinal tract environmental stress and better play the role of calf intestinal tract health. In order to achieve the above purpose, the embodiment of the invention adopts the following technical scheme: In a first aspect, the invention provides a chlorella pyrenoidosa CV-5 with a preservation number of CGMCC No.15206 and Latin name Chlorella pyrenoidosa. The chlorella pyrenoidosa CV-5 is separated from a water source of Hebei Shijia, the Latin name is Chlorella pyrenoidosa, the algae is preserved in China general microbiological culture Collection center (China Committee for culture Collection of microorganisms) in 1 month 12 of 2018, the preservation unit address is North Star Xiyu No.1, 3 in the Chaiyang region of Beijing, and the preservation number is CGMCC No.15206. The invention discovers that compared with other algae, the chlorella pyrenoidosa CV-5 provided by the invention can more effectively improve the survival rate of probiotics in calf gastric juice, and in addition, compared with the combination of other algae and probiotics, the chlorella pyrenoidosa CV-5 provided by the invention can more obviously reduce the abundance of pathogenic bacteria in intestinal tracts of diarrhea calves. Therefore, the chlorella pyrenoidosa CV-5 has good application prospect in improving calf intestinal health. In a second aspect, the invention provid