CN-121975626-A - Microalgae Y-1 and culture method and application thereof

Abstract

The invention relates to the technical field of microorganisms, in particular to microalgae Y-1, a culture method and application thereof. The invention discovers and identifies a strain of green hammer algae microalgae Y-1 with a preservation number of CCTCC NO: M20252903. The gene sequence of the microalgae Y-1 is compared with the published algae gene sequence, and the comprehensive analysis of the photomicrograph is combined, so that the microalgae Y-1 is confirmed to be a new genus Chloroidium. The successful separation and culture of the microalgae Y-1 not only provides ideal materials for the functional research of marine algae, but also lays a foundation for further exploring the symbiotic relationship between the microalgae and coral. In addition, the microalgae Y-1 has potential application value in the fields of biological energy sources (biodiesel and hydrogen production), environmental remediation (wastewater treatment and CO 2 fixation), agriculture and aquatic products (biofertilizer and feed additive) and the like.

Inventors

• SU HANG
• YU JIECAN
• DAI YULING
• WEI LI

Assignees

• 海南师范大学

Dates

Publication Date

20260505

Application Date

20260209

Claims (10)

1. 1. A strain of microalgae Y-1 (Chloroidium sp. Nov. Y-1) is characterized in that the preservation number of the microalgae Y-1 is CCTCC NO: M20252903.
2. 2. The method for culturing microalgae Y-1 according to claim 1, which comprises the step of inoculating the microalgae Y-1 into a culture medium for culturing.
3. 3. The culture method according to claim 2, wherein the medium is a modified f/2 liquid medium; the improved f/2 liquid medium comprises the following components in concentration: 75 mg/L NaNO 3 、1.5 g/L NaHCO 3 、8 mg/L KH 2 PO 4 、0.01 mg/L FeCl 3 ·6H 2 O、1 mL/L Complex trace element solution, 0.1 μg/L vitamin B 12 , 0.02 μg/L biotin, 0.5 μg/L folic acid, 50 μg/mL kanamycin, 100 μg/mL ampicillin, 50 μg/mL streptomycin, and 2.5 μg/mL amphotericin B.
4. 4. The method according to claim 2, wherein the temperature of the culture is 25 ℃, the light intensity is 200. Mu. Mol/(m2.s), the light period is 12h light and 12h dark.
5. 5. Use of microalgae Y-1 according to claim 1 for the preparation of a microbial preparation.
6. 6. A microbial preparation, which is characterized in that, the microbial preparation comprises the microalgae Y-1 of claim 1.
7. 7. Use of microalgae Y-1 according to claim 1 for studying the symbiotic relationship and mechanism of action of coral and green algae.
8. 8. The use of the microalgae Y-1 according to claim 1 or the microbial preparation according to claim 6 in any one or more of the following, (1) Producing energy sources; (2) Repairing the environment; (3) Producing a biological fertilizer; (4) Producing a feed additive; (5) Promoting growth.
9. 9. The use according to claim 8, wherein the energy source comprises biodiesel and/or hydrogen.
10. 10. The use according to claim 8, wherein the environmental remediation comprises wastewater treatment and/or CO 2 fixation.

Description

Microalgae Y-1 and culture method and application thereof Technical Field The invention relates to the technical field of microorganisms, in particular to microalgae Y-1, a culture method and application thereof. Background Spherical and ellipsoidal (chlorella-like) green algae can only asexually reproduce through motile autogenous spores or zoospores. These organisms are common and abundant inhabitants of both terrestrial and aquatic (mostly fresh water) environments. This form is also typical of many photosynthetic symbionts such as lichen. Green algae is an important ecological community, playing a key role as a primary producer, and is becoming a viable source of commercial compounds in the fuel, food and pharmaceutical industries. The species of the genus green hammer (Chloroidium) are widely distributed in different environments, mainly terrestrial and freshwater environments. In the past century, they were described by different specific and common names, according to their origin and morphology. They generally have a high phenotypic plasticity and are therefore easily adaptable to different environments. These species generally have high phenotypic plasticity, ellipsoidal cell shape, unequal autopores during propagation, minicell size and wall chloroplasts, and also have the biochemical ability to synthesize and accumulate the rather unusual polyol ribitol. The green hammer algae belongs to Chlorophyta (Chlorophyta) and Coccococcus (Trebouxiophyceae), and is a single-cell spherical microalgae. The cell morphology rule (diameter 2-10 μm) is that chloroplasts are of a typical cup-shaped structure and contain significant starch nuclei, which constitute the taxonomic features of the genus core. The green hammer algae species exhibit unique ecological fitness, most of which are exclusively distributed in sub-aerogenic environments (such as wet rock surfaces, bark and soil micro-interfaces) and facultative distributed in freshwater aquatic ecosystems. The plant has the characteristic of generalization distribution, and the ecological range covers temperate zones to tropical climate zones. Notably, some germplasm resources can initiate lipid super-accumulation mechanisms at the end of natural growth or under adverse stress (including high irradiance, osmotic pressure fluctuations, and nutrient limiting conditions). Typically representing, for example Chloroidium ellipsoideum, a total lipid content of 40-50% (w/w) of the dry cell weight under nitrogen stress makes it one of the most competitive microalgae candidates for the third generation biodiesel production. Chlorococcum ellipsoideum MACC 712 shows high endogenous gibberellin content, which can promote plant growth. In addition, the secondary metabolite diversity advantage is shown by a plurality of species, and the metabolite spectrum of the secondary metabolite diversity advantage comprises chlorophyll derivatives, polyunsaturated fatty acids, antioxidant polysaccharides and other functional components, so that the secondary metabolite diversity advantage has important development value in the fields of food additives and biological medicines. Therefore, the deep development of the algae strain of the green hammer algae with the biological growth promoting function has important significance for realizing the carbon neutralization target and enriching the application of microalgae resources. For example, in the aspect of agricultural application, the screened excellent green hammer algae strain can be prepared into an algae biological agent through fermentation culture, and then is soaked in seeds or sprayed on rice seedlings through leaf surfaces, so that plant root development and plant height growth can be promoted, and the chlorophyll content of leaves can be effectively improved. Disclosure of Invention The invention aims to provide microalgae Y-1, and a culture method and application thereof, so as to solve the problems in the prior art. In order to achieve the above object, the present invention provides the following solutions: The invention provides a microalgae Y-1 (Chloroidium sp.nov. Y-1), wherein the preservation number of the microalgae Y-1 is CCTCC NO: M20252903. The invention provides a culture method of microalgae Y-1, which comprises the step of inoculating the microalgae Y-1 into a culture medium for culture. Preferably, the culture medium is a modified f/2 liquid culture medium; the improved f/2 liquid medium comprises the following components in concentration: 75 mg/L NaNO3、1.5 g/L NaHCO3、8 mg/L KH2PO4、0.01 mg/L FeCl3·6H2O、1 mL/L Complex trace element solution, 0.1 μg/L vitamin B 12, 0.02 μg/L biotin, 0.5 μg/L folic acid, 50 μg/mL kanamycin, 100 μg/mL ampicillin, 50 μg/mL streptomycin, and 2.5 μg/mL amphotericin B. Preferably, the temperature of the culture is 25 ℃, the illumination intensity is 200 mu mol/(m 2. S), the photoperiod is 12 h light and 12 h dark. The invention provides application of the microalgae Y-1 in preparation