CN-121975628-A - Application of silkworm excrement as culture medium raw material for producing beauveria bassiana and method for producing beauveria bassiana by utilizing silkworm excrement waste

Abstract

The invention relates to application of silkworm excrement in serving as a culture medium raw material for producing beauveria bassiana, and a method for producing beauveria bassiana by utilizing silkworm excrement waste, belonging to the field of fermentation. The invention also provides a beauveria bassiana solid culture medium which is characterized in that the culture medium raw material contains silkworm excrement. The invention provides a fermentation culture technology utilizing waste silkworm excrement, which aims to improve the production efficiency of beauveria bassiana and provide a new resource utilization mode for the production of the beauveria bassiana. The technology can not only effectively reduce the environmental pollution of wastes, but also improve the production efficiency, save the raw material cost and improve the quality of products.

Inventors

• LI MIN
• Fan Heling
• ZHENG FENGHUA
• XIE SHIYU
• FAN XIAOYAN
• ZHOU YANGMING
• LIU SHULIN

Assignees

• 成都中医药大学
• 四川智佳成生物科技有限公司

Dates

Publication Date

20260505

Application Date

20260409

Claims (9)

1. 1. The application of faeces Bombycis in the preparation of culture medium for Beauveria bassiana is provided.
2. 2. A beauveria bassiana solid culture medium is characterized in that the culture medium raw material contains silkworm excrement.
3. 3. The solid culture medium of beauveria bassiana according to claim 2, which is characterized by comprising the following raw materials in parts by weight: 1-9 parts of rice and 1-9 parts of silkworm excrement.
4. 4. The solid culture medium of beauveria bassiana according to claim 3, which is characterized by comprising the following raw materials in parts by weight: 7 parts of rice and 3 parts of silkworm excrement.
5. 5. A method for preparing the beauveria bassiana solid medium according to claim 3 or 4, which is characterized by comprising the following steps: a. weighing the raw materials in weight proportion; b. Adding carbon and nitrogen source and water, adding into a tray filled with solid culture medium, mixing, sterilizing with high pressure steam, and cooling to room temperature.
6. 6. The method for preparing a solid culture medium of beauveria bassiana according to claim 5, wherein the carbon-nitrogen source is 0.4% of glucose and potassium nitrate.
7. 7. A method for producing beauveria bassiana by using silkworm excrement waste is characterized in that beauveria bassiana solid culture medium of any one of claims 2-4 is adopted to cultivate silkworm excrement waste to produce beauveria bassiana.
8. 8. The method for producing beauveria bassiana by using silkworm excrement waste as set forth in claim 7, wherein the method comprises the following steps: a. Activating strain, namely activating and culturing by adopting inclined plane strain; b. Preparing liquid inoculating liquid by liquid seed enlarging culture process; c. Preparing a solid culture medium, namely weighing raw materials in weight ratio, adding a carbon-nitrogen source and water, adding the raw materials into a tray filled with the solid culture medium, uniformly mixing, sterilizing by high-pressure steam, and cooling to room temperature for later use; d. The solid state fermentation and spore production are carried out under the conditions of 26 ℃ and RH=85%, for 10 days, wherein the first 3 days are dark culture and the second 7 days are open illumination culture, and after the culture is finished, the beauveria bassiana spore powder is obtained by continuously drying and sieving under the condition of 35 ℃.
9. 9. The method for producing beauveria bassiana by utilizing silkworm excrement waste according to claim 8, wherein the method comprises the following steps: the strain activation method in the step a comprises the steps of taking out purified inclined plane strains in a refrigerator at the temperature of 4 ℃, inoculating the purified inclined plane strains in an SDY solid culture medium by a streaking method, reversely placing the strain in a constant temperature incubator at the temperature of 26 ℃ for half-illumination culture for 14 days, and collecting the strain for standby when the culture medium grows light yellow-white conidium powder, wherein the illumination condition L is D=12:12; the liquid inoculation liquid preparation method comprises the steps of preparing an SDY liquid culture medium, subpackaging and sterilizing, inoculating spore suspension to enable the initial concentration to reach 2.4X10 6 spores/mL, and then carrying out shaking culture on a shaking table at 26.2 ℃ and 174r/min for 72 hours, wherein the SDY liquid culture medium comprises the following raw materials in percentage by weight, 4% of glucose, 1% of yeast extract, 1% of peptone and 0.05% of Tween-80.

Description

Application of silkworm excrement as culture medium raw material for producing beauveria bassiana and method for producing beauveria bassiana by utilizing silkworm excrement waste Technical Field The invention relates to application of silkworm excrement in serving as a culture medium raw material for producing beauveria bassiana, and a method for producing beauveria bassiana by utilizing silkworm excrement waste, belonging to the field of fermentation. Background In the production process of the medicinal stiff silkworm (Bombyx batryticatus), the silkworm excrement is usually discarded as waste, but the waste actually contains abundant resources, and if the waste can be reasonably utilized, more sustainability and economic benefit can be brought to the production process. Li H, Xu X, Zhang M, et al. Accelerated degradation of cellulose in silkworm excrement by the interaction of housefly larvae and cellulose-degrading bacteria[J]. Journal of environmental management, 2022, 323: 116295. In the literature, it is reported that silkworm excrement is discarded as waste in the stiff silkworm production process, and potential environmental hazards exist. As the silkworm excrement contains a large amount of cellulose which is difficult to degrade, if the silkworm excrement is directly applied to soil, toxic substances such as organic acid, hydrogen sulfide (H2S), nitric Oxide (NO) and the like in the soil can be increased, and the plant root system can be damaged, so that seed germination and plant growth are affected. In addition, silkworm excrement may contain various pathogenic microorganisms such as nuclear polyhedrosis virus, bacillus thuringiensis, beauveria bassiana and the like, and if the pathogens are not treated effectively, the pathogens can be transmitted to the surrounding environment, so that the health and production of silkworms are affected, and the quality and yield of silkworm cocoons are reduced. Therefore, reasonable utilization and treatment of silkworm excrement is important to reduce its negative impact on the environment and agricultural production. In recent years, as the concepts of environmental protection and resource recycling are increasingly paid attention to, how to effectively treat and reuse such wastes has become an urgent problem to be solved. Faeces Bombycis as faeces Bombycis larva usually contains higher organic matter, and is a potential high quality culture medium resource. In the conventional stiff silkworm production, the utilization of silkworm excrement is generally neglected. CN201610107181, method for cleaning and utilizing silkworm excrement waste in quick-decay paddy field and silkworm excrement biological decay promoter. The fast decomposing technology for silkworm excrement waste is provided, and the fast decomposing technology is used as base fertilizer and tillering fertilizer for rice field to treat silkworm excrement waste and convert the silkworm excrement waste into organic fertilizer effectively. The technical scheme of the patent comprises the collection of silkworm excrement waste, the preparation and the application of silkworm excrement biological decay promoter, the rapid decay treatment of silkworm excrement waste and the application of fermented silkworm excrement as fertilizer. The silkworm excrement biological decay promoter consists of a plurality of active bacteria and a nutrition carrier, can reduce the generation of harmful gas through the synergistic effect of microorganisms, effectively kills silkworm excrement germs and promotes the conversion and the recycling of silkworm excrement organic fertilizer. However, this patent has the disadvantage that the ① treatment process is less flexible, and although it proposes a rapid decay method, the method relies on weather conditions, especially within 10 to 15 days prior to rice transplanting, and requires the selection of a sunny day for operation. This limitation can affect the versatility of the process, particularly when applied in areas of heavy moisture or weather instability, which may not be easy to implement. ② The rapid decomposition method disclosed by the patent relates to a large number of operation steps such as silkworm excrement stacking, film covering sealing and the like, so that the technology has a certain difficulty in popularization in small-scale farmers. Although this method is environmentally friendly, it requires a lot of manpower and time to perform. ③ The core technology of the patent relies on silkworm excrement biological decay promoting agent and auxiliary materials such as rice bran, peanut shells and the like, and the materials can improve the treatment effect to a certain extent, but also increase the cost. For small farmers, the cost of raw materials can be a burden and large scale applications are not easily realized. CN201910404362 beauveria bassiana strain and its culture method. Introduces a beauveria bassiana (Beauveria bassiana) ZD-1 strain and a culture me