CN-121975630-A - Edible fungi and application of edible fungi and trichoderma in synergistic inhibition of aspergillus flavus

Abstract

The invention discloses edible fungi and application of the edible fungi and the trichoderma in synergistic inhibition of aspergillus flavus, and belongs to the technical field of microorganisms. According to the invention, the trichoderma library with antagonistic capability is separated from the waste mushroom substrate through the three-stage screening system, wherein two trichoderma strains associated with the pleurotus geesteranus have outstanding aspergillus flavus antagonistic effect, and the aspergillus flavus growth and the toxigenic can be more effectively inhibited under the cooperation of the pleurotus geesteranus. Furthermore, the synergistic system of the pleurotus geesteranus and the trichoderma viride can effectively prevent and control the pollution loss of aflatoxin B1 caused by aspergillus flavus infection in peanuts, and has the effect of promoting the growth of the peanuts. The invention can recycle the waste edible fungus matrix, not only realize the resource utilization of agricultural wastes, but also fill the blank in the field of biological detoxification, so as to improve the input-output ratio of agriculture. Therefore, the invention has good application prospect.

Inventors

• GAO JING
• XU JIAYU
• ZHU HAN
• Zha Manqian
• ZHANG TIANYU
• DONG ANQI
• LI JIAYI
• Antonio Francesco Logrico

Assignees

• 湘湖实验室(农业浙江省实验室)

Dates

Publication Date

20260505

Application Date

20251219

Claims (10)

1. 1. The application of edible fungi and the associated trichoderma in the synergistic inhibition of aspergillus flavus is characterized in that the associated trichoderma comprises Ha Cimu mould T. Harzianum and/or trichoderma longibrachiatum T. Longibrachiatum; The preservation number of Ha Cimu mould is CGMCC No.42339, and the preservation number of trichoderma longibrachiatum is CGMCC No. 42340.
2. 2. The use according to claim 1, wherein the edible mushrooms comprise pleurotus geesteranus.
3. 3. The use according to claim 1, wherein the use comprises decreasing the growth rate of Aspergillus flavus and decreasing the synthesis of aflatoxin B1.
4. 4. The method of claim 1, wherein the method comprises synergistically controlling Aspergillus flavus contamination and promoting crop growth.
5. 5. The use of claim 4, wherein the crop comprises peanut.
6. 6. A method for inhibiting the growth of aspergillus flavus and the generation of toxins is characterized by comprising the steps of adding an edible fungus fruiting body extract, ha Cimu mould and/or trichoderma longibrachiatum into an environment containing aspergillus flavus, and carrying out inhibition treatment on the aspergillus flavus; the preservation number of Ha Cimu mould is CGMCC No. 42339, and the preservation number of trichoderma longibrachiatum is CGMCC No. 42340.
7. 7. The method of claim 6, wherein the edible fungus comprises pleurotus geesteranus.
8. 8. A method for preventing and controlling aflatoxin pollution of crops is characterized by comprising the step of adding an edible fungus culture medium and Ha Cimu mould into the cultivation environment of the crops, wherein the preservation number of the Ha Cimu mould is CGMCC No. 42339.
9. 9. The method of claim 8, wherein the edible fungus comprises pleurotus geesteranus and the crop comprises peanut.
10. 10. A composite preparation is characterized by comprising Ha Cimu mould with a preservation number of CGMCC No. 42339, trichoderma longibrachiatum with a preservation number of CGMCC No. 42340, and a culture medium or fruiting body extract of pleurotus geesteranus.

Description

Edible fungi and application of edible fungi and trichoderma in synergistic inhibition of aspergillus flavus Technical Field The invention relates to the technical field of microorganisms, in particular to edible fungi and application of the edible fungi and the trichoderma in synergistic inhibition of aspergillus flavus. Background Mycotoxins are toxic secondary metabolites produced by certain fungi (mould) in a proper environment, mainly pollute agricultural products such as grains, nuts, feeds and the like, and the toxins form serious threats to human and animal health, can cause acute poisoning, immunosuppression, carcinogenesis, teratogenesis and other damages, and can also cause crop quality degradation and agricultural economic loss. The common mycotoxin is aflatoxin, which is a secondary metabolite with extremely strong carcinogenicity and toxicity mainly generated by fungi such as aspergillus flavus (Aspergillus flavus) and aspergillus parasiticus, and is especially commonly found in agricultural products such as peanuts, corns, cottonseeds, nuts and the like which are improperly stored in warm and humid environments, and is one of the most toxic and most harmful mycotoxins known at present, wherein the toxicity and carcinogenicity of aflatoxin B1 (Aflatoxin B 1,AFB1) are most severe, and the aflatoxin B1 is listed as a first-class carcinogen by the world health organization. Aspergillus flavus is also a plant pathogenic bacterium, and infection of crop root systems can induce aspergillosis, spike rot and the like, and has double hazards. Prevention and reduction of fungal and toxin contamination is divided into physical, chemical and biological means. The traditional detoxification modes such as adsorption, ultraviolet irradiation, chemical reagent degradation and the like have high cost and destroy food nutrition components, and the biological strategy realizes bacteriostasis and toxicity control through species competition, signal interference, enzymatic degradation and the like, so that the method has the advantages of being green and efficient. Trichoderma (Trichoderma) is one of the three biological control microbial resources worldwide, widely exists in soil and sapropel, has the characteristics of strong adsorbability cell structure, secretion of functional factors and the like, and has been developed into pesticides, fertilizers, somatotropins, soil restoration agents and the like. However, most of the commercial microbial agents have complex active ingredients, insufficient colonization ability, severe application conditions and poor stability, and no product specific to aspergillus flavus exists at present. On the other hand, the large mushroom (mushroom) which is the same as fungi has been developed into a fifth class of agricultural products which are inferior to grains, oils, vegetables and fruits, and the yield of the large mushroom (mushroom) is more than 70% of the total global yield in the first edible mushroom production and export of the large country in the world. But mass production is accompanied by a large amount of waste mushroom culture medium (spent mushroom substrate, SMS) in a ratio of 1:3.25-5, and the main components are wood chips, straws, corncobs and the like, and also contain functional secretion of mushrooms. Most of the waste mushroom culture medium is directly discarded and burned, and the rest is used as feed for recycling, so that serious environmental pollution and resource waste are caused. Therefore, sustainable utilization of edible mushrooms and waste culture matrixes thereof in the field of food safety has become a key direction for promoting development of the edible mushroom industry. In view of this, the present invention has been made. Disclosure of Invention The invention aims to provide edible fungi and application of the edible fungi and the trichoderma in synergistic inhibition of aspergillus flavus so as to solve the technical problems. The invention is realized in the following way: In a first aspect, the invention provides an edible fungus comprising pleurotus geesteranus Pleurotus pulmonarius and an application of the edible fungus and the companion trichoderma in synergistically inhibiting aspergillus flavus, wherein the companion trichoderma comprises Ha Cimu mould t. The Ha Cimu mould Trichoderma harzianum provided by the invention has a preservation number of CGMCC No.42339, a preservation time of 2025, 12 months and 04 days, a preservation center of China general microbiological culture Collection center, a preservation address of North Star Xiyu No. 1, 3 in the Korean region of Beijing, and a microbiological institute of China academy of sciences. The preservation number of the trichoderma longibrachiatum Trichoderma longibrachiatum provided by the invention is CGMCC No. 42340, the preservation time is 2025, 12 months and 04 days, the preservation center is the China general microbiological culture Collection center, and the preservati