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CN-121975631-A - Marine fungus fusarium hornii and application thereof

CN121975631ACN 121975631 ACN121975631 ACN 121975631ACN-121975631-A

Abstract

The invention discloses a marine fungus fusarium horneri and application thereof. The inventor screens and obtains a strain of cutin fusarium capable of efficiently producing ligusticum wallichii from marine sediments, the content of the produced ligusticum wallichii reaches 561.7 mug/L after fermentation in GPY liquid culture medium, a possibility and a foundation are provided for breeding strains which can be suitable for industrialized mass production of the ligusticum wallichii in the future, and mass spectrum, hydrogen spectrum and carbon spectrum data of the separated ligusticum wallichii are completely consistent with ligusticum wallichii data reported in literature.

Inventors

  • LAN WENJIAN
  • LI JIAMING
  • LAI LIXIAN
  • LI HOUJIN
  • WANG SHUANGXU
  • HU WENHAO
  • LIU XI
  • CHEN XINZI

Assignees

  • 中山大学
  • 华慧海洋多糖生物技术(深圳)有限公司

Dates

Publication Date
20260505
Application Date
20251231

Claims (8)

  1. 1. A strain of fusarium hornii, characterized in that: The deep sea sediment sample from south China sea is obtained through enrichment culture, separation and purification, is named Fusarium keratoplasticum LWJ2314, has a preservation number of GDMCC 67094 and is preserved in the microorganism strain preservation center of Guangdong province at 10 months 13 of 2025, and the preservation unit is the No. 59 building of the No. 100 institute in Guangzhou city martyr.
  2. 2. The fusarium hornii of claim 1, wherein: the colony form of the fusarium horneticum on the PDA plate is white mycelium.
  3. 3. The fusarium hornii of claim 1, wherein: the nucleotide sequence of the ITS of the fusarium kerateum is shown as SEQ ID NO. 1.
  4. 4. The fusarium hornii of claim 1, wherein: The fusarium cuticum can produce ligusticum wallichii.
  5. 5. The use of fusarium cuticum according to any one of claims 1 to 4 for the production of ligusticum wallichii.
  6. 6. A method for producing ligusticum wallichii is characterized by comprising the following steps: Inoculating Fusarium horniae into a liquid culture medium for fermentation culture, separating mycelia and fermentation liquor after the culture is finished, extracting the fermentation liquor by using ethyl acetate to obtain an extract A, airing the separated mycelia, extracting the mycelia by using methanol, concentrating the mycelia to obtain an extract B, combining the extract A and the extract B, and purifying the extract B to obtain the ligusticum wallichii.
  7. 7. The method for producing ligusticum wallichii according to claim 6, wherein: The liquid culture medium is GPY liquid culture medium.
  8. 8. The method for producing ligusticum wallichii according to claim 6, wherein: The fermentation culture condition is that the fermentation culture is carried out for 40-50 d at the temperature of 27-29 ℃.

Description

Marine fungus fusarium hornii and application thereof Technical Field The invention relates to the field of microbial engineering, in particular to marine fungus fusarium horniness and application thereof. Background Ligusticum wallichii (Ligusticum wallichii III alkali, perlolyrine, PL) is one of the effective active alkaloids of traditional Chinese medicine Ligusticum wallichii, the chemical structure of which is 1- (5-hydroxymethyl-2-furyl) -9H-pyrido [3,4-b ] indole, belonging to beta-carboline (beta-Carboline) alkaloid. The structure is characterized in that a pyridoindole core is connected with a furan ring through a carbon bond, and pyrido [3,4-b ] indole serving as a core skeleton is taken as a main active group. Modern pharmacological researches show that the ligusticum wallichii has the characteristics of multi-target and multi-channel effects, and has obvious activity in the fields of cardiovascular and cerebrovascular protection, anti-fibrosis, neuroprotection and the like. Ligusticum wallichii is mainly derived from traditional Chinese medicine Ligusticum wallichii. Other plants such as ryegrass and polygala are also present, but at a much lower level than ligusticum wallichii. The content of natural ligustilide in ligusticum wallichii is relatively low (lower than 0.1%), the use of traditional Chinese medicine ligusticum wallichii to extract ligusticum wallichii is easy to cause that medicinal materials cannot be fully utilized and the cost is high, the plant growth period is long, and the extraction process is complex, so that the production of ligusticum wallichii is difficult. The process for extracting ligusticum wallichii from secondary metabolite of microorganism has the characteristics of low cost, rapid propagation, relatively simple extraction process and the like. Moreover, only a small number of microorganisms have been reported to produce ligusticum wallichii, so the invention provides a possibility and basis for the future breeding of strains which can be suitable for the industrialized mass production of ligusticum wallichii. The ocean contains rich biological resources, so that the ocean organisms evolve genes, enzymes and metabolic pathways different from those of land organisms in order to adapt to the special living environment of the ocean, and can generate metabolic products with novel and unique biological activities in chemical structures, thereby becoming rich sources of drug lead compounds and having very wide development prospects. Disclosure of Invention The invention aims to overcome the defects and shortcomings of the prior art and provides a marine fungus fusarium horneri. It is another object of the present invention to provide the use of the marine fungus Fusarium keratitis. The aim of the invention is achieved by the following technical scheme: Fusarium hornii (Fusarium keratoplasticum) is obtained from deep sea sediment samples collected from south China sea through enrichment culture, separation and purification, and is named Fusarium keratoplasticumLWJ2314. Fusarium hornbeam has a preservation number GDMCC 67094 and is preserved in the microorganism strain preservation center of Guangdong province at the 10 th month 13 of 2025, and the preservation unit is the No. 59 building 5 of the No. 100 institute of Guangzhou city martyr. The bacterial colony of the fusarium horneticum on the PDA plate is white mycelium, and the whole plate can be paved about 5-7 days. The nucleotide sequence of the ITS of the fusarium kerateum is shown as SEQ ID NO. 1. The fusarium hornbeam can produce ligusticum wallichii (perlolyrine). The structure formula of the ligusticum wallichii is shown as formula I: I is a kind of The application of the fusarium cuticum in the production of ligusticum wallichii. A method for producing ligusticum wallichii indole comprises the following steps: Inoculating Fusarium horniae into a liquid culture medium for fermentation culture, separating mycelia and fermentation liquor after the culture is finished, extracting the fermentation liquor by using ethyl acetate to obtain an extract A, airing the separated mycelia, extracting the mycelia by using methanol, concentrating the mycelia to obtain an extract B, combining the extract A and the extract B, and purifying the extract B to obtain the ligusticum wallichii. The liquid culture medium is GPY liquid culture medium. The GPY liquid culture medium comprises 15g of glucose, 10g of peptone, 2g of yeast extract, 20g of sea salt, 2g of L-tryptophan and 1000mL of water. The fermentation culture condition is that the fermentation culture is carried out for 40-50 d at the temperature of 27-29 ℃. The purification is performed by semi-preparative HPLC after separation by silica gel column chromatography. Compared with the prior art, the invention has the following advantages and effects: The inventor screens and obtains a strain of cutin fusarium capable of efficiently producing ligusticum wallichii from marine