Search

CN-121975632-A - Efficient lignocellulose degradation composite strain and application thereof in methane production from fermented straw

CN121975632ACN 121975632 ACN121975632 ACN 121975632ACN-121975632-A

Abstract

The invention discloses a high-efficiency lignocellulose degrading composite strain and application thereof in methane production from fermented straws, wherein the lignocellulose degrading composite strain consists of Phanerochaete chrysosporium, oyster mushroom, phoenix mushroom and panus pinophilum. When the composite strain is used for preprocessing the straws, the cellulose degradation rate in the straws reaches 77.86%, the hemicellulose degradation rate reaches 21.01%, the lignin degradation rate reaches 34.85%, and when the preprocessed straws and the feces are subjected to anaerobic fermentation together, compared with a control group, the total gas production rate is improved by 86.28%, and the methane production rate is improved by 98.95%. Therefore, the invention provides a green and efficient straw recycling technology, so as to synchronously realize the purposes of waste reduction, energy recovery and fermentation residue recycling.

Inventors

  • WANG JIE
  • WU JIANLIN
  • Lian Lingdan
  • Wang saibo

Assignees

  • 华南农业大学

Dates

Publication Date
20260505
Application Date
20251231

Claims (10)

  1. 1. A lignocellulose degrading composite strain is characterized by comprising Phanerochaete chrysosporium (Phanerochaete chrysosporium), oyster mushroom (Pleurotus ostreatus), phoenix mushroom (Pleurotus sajor-caju) and panus pinophilum (Talaromyces pinophilus), wherein the panus pinophilum is preserved in the microorganism strain preservation center (GDMCC) of Guangdong province at the 12 th month of 2025, and the preservation number is GDMCC NO:67474.
  2. 2. A microbial inoculum is characterized in that, the microbial inoculum comprises the lignocellulose degrading composite strain according to claim 1.
  3. 3. The lignocellulose degrading composite strain of claim 1 or the microbial inoculum of claim 2, wherein the live bacterial count ratio of Phanerochaete chrysosporium, oyster mushroom, pleurotus sajor-caju is 1:1:1.8-2.2.
  4. 4. Use of a lignocellulose degrading composite strain according to claim 1 or a microbial agent according to claim 2 for degrading cellulose, hemicellulose and/or lignin.
  5. 5. Use of a lignocellulose degrading composite strain according to claim 1 or a microbial agent according to claim 2 for the preparation of cellulases, hemicellulases and/or ligninases.
  6. 6. Use of the lignocellulose degrading composite strain of claim 1 or the microbial inoculum of claim 2 for degrading straw.
  7. 7. Use of the lignocellulose degrading composite strain of claim 1 or the microbial inoculum of claim 2 in methane production from fermented straw.
  8. 8. A method for producing methane by fermenting straw, which is characterized by comprising the following steps: S1, pretreatment of straw, namely inoculating the lignocellulose degrading composite strain of claim 1 or the microbial inoculum of claim 2 into straw for fermentation treatment; S2, mixing the pretreated straws with animal manure, and performing anaerobic fermentation.
  9. 9. The method according to claim 8, wherein the inoculation amount in step S1 is 4-6%.
  10. 10. The method according to claim 8, wherein the dry matter ratio of the pretreated straw to the animal manure in step S2 is 1:1.8-2.2.

Description

Efficient lignocellulose degradation composite strain and application thereof in methane production from fermented straw Technical Field The invention belongs to the technical field of microbial fermentation, and particularly relates to a high-efficiency lignocellulose degradation composite strain and application thereof in methane production from fermented straws. Background The Chinese is a large agricultural country, a large amount of crops such as rice, corn, soybean and the like are planted, and the crop cultivation method has rich straw reserve resources. The straw is not reasonably utilized as a recyclable resource, is difficult to degrade under natural conditions and has a longer degradation period. Untreated straw may have a certain influence on the physicochemical properties of soil and farmland cultivation, and greatly restricts the development of straw returning technology. Traditional straw degradation methods are classified into physical methods, chemical methods and biological methods. The physical method has simple operation, no secondary pollution but high energy consumption, the chemical method has high efficiency, quick reaction but easy generation of pollutants and higher cost. Compared with the biological method, the biological method uses microorganisms (bacteria, fungi and the like) or enzymes as cores, can effectively accelerate the degradation speed of organic solid wastes rich in fibers such as straws and the like, does not need chemical reagents, has no secondary pollution, meets the environmental protection requirement, and has high utilization rate because the microbial degradation products are mainly natural substances such as saccharides, amino acids and the like, and can be easily used as feed, fertilizer or biological energy raw materials. At present, the anaerobic fermentation technology is used for converting organic matters such as cellulose and hemicellulose in straws into clean energy such as methane, and the like, so that the method has become an important direction for recycling agricultural wastes. The effect of oyster mushroom-treated wheat and rice straw on fiber components and in-vitro rumen fermentation characteristics is described in one article, that oyster mushroom-treated wheat straw significantly reduces the acid washing lignin content, and significantly improves the total gas yield, dry matter degradation rate and methane yield (+26.1%) of 72 h. In the research on methane production from wheat straw pretreated by Phanerochaete chrysosporium, it is pointed out that the wheat straw is pretreated by Phanerochaete chrysosporium (Phanerochaete chrysosporium), the degradation rates of lignin, hemicellulose and cellulose in the straw after 20 days are 62.75 percent, 17.7 percent and 10.9 percent respectively, and the methane production rate of the straw degradation treatments by Phanerochaete chrysosporium is improved by 42 percent and 41 percent respectively compared with the comparison. Therefore, the method further screens lignocellulose degradation and fermentation straw methanogens with higher efficiency, and has important significance for high-efficiency and high-valued comprehensive utilization of agricultural wastes. Disclosure of Invention The invention aims to overcome the defects and the shortcomings in the prior art and provide a lignocellulose degrading composite strain. The mutual synergistic effect among all strains can efficiently and stably degrade lignocellulose. The second object of the invention is to provide a microbial inoculum comprising the lignocellulose degrading composite strain. The third object of the invention is to provide the application of the lignocellulose degrading composite strain and the microbial inoculum in degrading cellulose, hemicellulose and/or lignin, preparing cellulase, hemicellulase and/or lignin enzyme, degrading straw or fermenting straw to produce methane. The fourth object of the invention is to provide a method for producing methane by fermenting straw. The above object of the present invention is achieved by the following technical solutions: The invention provides a lignocellulose degrading composite bacterial strain, which consists of Phanerochaete chrysosporium (Phanerochaete chrysosporium), oyster mushroom (Pleurotus ostreatus), phoenix mushroom (Pleurotus sajor-caju) and panus pinophilum (Talaromyces pinophilus), wherein the panus pinophilum Talaromyces pinophilus is preserved in the microorganism strain preservation center (GDMCC) of Guangdong province at 12 months of 2025, and the preservation number is GDMCC NO:67474. The invention utilizes two lignocellulose degrading bacteria obtained by earlier stage screening work, namely panus pinophilum and panus funiculosus, and combines the combination research of various filamentous fungi preserved in the laboratory to form a lignocellulose degrading composite bacterial system. Hemicellulose and cellulose in natural biomass (such as straw) are interwoven to for