CN-121975634-A - Trichoderma reesei HK1-12 and secondary metabolite and application thereof

Abstract

The invention belongs to the field of fungus secondary metabolites and application thereof, and in particular relates to trichoderma reesei HK1-12, a secondary metabolite thereof and application thereof, wherein the preservation unit is China general microbiological culture Collection center (CGMCC) with the preservation number of CGMCC No. 41951. The trichoderma HK1-12 has excellent characteristics in the aspects of growth promotion, plant protection and the like, and is expected to replace the existing similar products to be popularized and applied.

Inventors

• CHEN MIN
• WANG JUANJUAN
• CHEN LEI
• SHEN LI
• ZHANG LIKUI

Assignees

• 扬州大学

Dates

Publication Date

20260505

Application Date

20260129

Priority Date

20250623

Claims (10)

1. 1. The Trichoderma reesei HK1-12 is characterized in that the strain preservation unit is China general microbiological culture Collection center with the preservation number of CGMCC No. 41951.
2. 2.A microbial agent comprising the Trichoderma reesei HK1-12 and/or a spore suspension thereof according to claim 1 as an active ingredient.
3. 3. A crude extract of trichoderma reesei HK1-12 as claimed in claim 1, characterized in that the crude extract of trichoderma reesei HK1-12 is useful for controlling rice sheath blight pathogen, wheat sheath blight pathogen.
4. 4. Use of trichoderma reesei HK1-12 according to claim 1 for the preparation of compounds 1,2, 3, 4 and/or 5, said compounds 1,2, 3, 4, 5 having the structure: 。
5. 5. a Sorbicillinoid compound or a salt thereof, which is characterized in that the Sorbicillinoid compound has a structure shown in the following compound 1: 。
6. 6. a composition characterized in that one or more of the compounds 1, 2,3, 4, 5 prepared in claim 4 or the crude extracts of trichoderma reesei HK1-12 in claim 3 are used as active ingredients.
7. 7. The use of one or more of the crude extracts of the compounds 1, 2,3, 4,5 prepared in claim 4 or the trichoderma reesei HK1-12 in claim 3 for controlling agricultural diseases, preferably caused by rice sheath blight pathogen, wheat sheath blight pathogen.
8. 8. Use of trichoderma reesei HK1-12 (and/or spore suspension thereof) according to claim 1, a microbial agent according to claim 2 or a composition according to claim 6 for plant protection.
9. 9. The method according to claim 8, wherein the plant is selected from the group consisting of wheat scab, potato early blight, tobacco brown rot, wheat take-all, rice bakanae, rape sclerotium, pepper phytophthora, rice blast, peanut southern blight, cucumber target spot, rice sheath blight and tomato early blight.
10. 10. Use of trichoderma reesei HK1-12 (and/or spore suspension thereof) according to claim 1 or a microbial agent according to claim 2 for promoting seed germination and/or plant growth.

Description

Trichoderma reesei HK1-12 and secondary metabolite and application thereof Technical Field The invention belongs to the field of fungus secondary metabolites and application thereof, and particularly relates to trichoderma reesei HK1-12, and a secondary metabolite and application thereof. Background Trichoderma reesei of terrestrial origin is widely used in the field of industrial enzyme production. Extreme temperature, salinity, pressure and pH variations in the marine environment allow the marine Trichoderma reesei to develop unique metabolic mechanisms and produce structurally diverse compounds. According to the invention, research on the trichoderma reesei HK1-12 from mangrove shows that the trichoderma reesei HK has excellent functions in aspects of growth promotion, plant protection and the like, and simultaneously, a large amount of Sorbicillinoid compounds can be produced. Disclosure of Invention The invention provides Trichoderma reesei HK1-12, which is characterized in that the strain preservation information comprises the preservation unit name of China general microbiological culture Collection center, the preservation unit address of China national academy of sciences of China, including North Star, no. 1, no. 3, the microbiological institute of China, the preservation date of 2025, 5 and 23, the preservation number of CGMCC No. 41951 and the classification name of Trichoderma reesei Trichoderma reesei. Another embodiment of the present invention provides a crude extract of Trichoderma reesei HK1-12, characterized in that the crude extract of Trichoderma reesei HK1-12 is useful for controlling rice sheath blight pathogen and wheat sheath blight pathogen. Another embodiment of the present invention provides the use of Trichoderma reesei HK1-12 in the preparation of compounds 1,2, 3, 4 and/or 5, wherein the compounds 1,2, 3, 4, 5 have the following structure: 。 Another embodiment of the present invention provides a Sorbicillinoid-type compound or a salt thereof, which is characterized in that the Sorbicillinoid-type compound has a structure as shown in the following compound 1: 。 another embodiment of the present invention provides a composition characterized in that the composition comprises one or more of the compounds 1,2, 3, 4, 5 or crude extracts of Trichoderma reesei HK1-12 as an active ingredient. The application of one or more of the compounds 1, 2,3, 4, 5 or the crude extracts of Trichoderma reesei HK1-12 in preventing and treating agricultural diseases. The agricultural disease is preferably caused by rice sheath blight pathogen, wheat sheath blight pathogen. Another embodiment of the present invention provides a microbial agent characterized in that the microbial agent comprises the above Trichoderma reesei HK1-12 (and/or spore suspension thereof) as an active ingredient. Another embodiment of the present invention provides the use of Trichoderma reesei HK1-12 (and/or spore suspension thereof) or a microbial agent or composition as described above in plant protection, in particular in controlling diseases caused by agricultural pathogenic fungi. The agricultural pathogenic fungi are selected from wheat scab original bacteria, potato early blight original bacteria, tobacco brown rot original bacteria, strawberry gray mold original bacteria, peach brown rot original bacteria, wheat take-all original bacteria, rice bakanae original bacteria, rape sclerotium original bacteria, pepper phytophthora capsici original bacteria, rice blast original bacteria, peanut southern blight original bacteria, cucumber target spot original bacteria, rice sheath blight original bacteria and tomato early blight original bacteria. Another embodiment of the present invention provides the use of Trichoderma reesei HK1-12 (and/or spore suspension thereof) or microbial agents as described above for promoting seed germination and/or plant growth. Compared with the prior art, the invention has the advantages that the trichoderma from the ocean is selected for the first time, and the trichoderma has excellent characteristics in aspects of growth promotion, plant protection and the like, and is hopeful to replace the existing similar products to be popularized and applied. Drawings FIG. 1 is a diagram showing colony morphology of Trichoderma reesei HK1-12 of the present invention cultivated in opposition to 14 agricultural pathogenic bacteria, wherein the upper side of each plate is the opposing cultivated group, the lower side is the blank control group, namely, only pathogenic bacteria are inoculated, trichoderma reesei HK1-12 is not inoculated, and the left and right sides of one plate are the front and the back side of each plate. FIG. 2 is a graph showing the inhibition ratio of Trichoderma reesei HK1-12 of the present invention against 8 pathogenic bacteria. FIG. 3 is a graph showing the inhibition ratio of Trichoderma reesei HK1-12 of the present invention to 6 pathogenic bacteria. FIG. 4 is a germination char