CN-121975635-A - Bacterial strain for degrading alternaria alternata toxin and application thereof

Abstract

The application discloses a strain for degrading alternaria alternata toxin and application thereof, and relates to the technical field of microorganisms. The disclosed biocontrol strain MM14-1 is identified as trichoderma, the trichoderma strain MM14-1 has a good inhibition effect on the growth of the alternaria alternata in a degradation test on a liquid culture medium, the degradation rate reaches 96%, meanwhile, the degradation rate of the intracellular crude extract of the trichoderma strain MM14-1 on the alternaria alternata toxin is 98.04%, the intracellular crude extract of the trichoderma strain MM14-1 can inhibit the synthesis of the alternaria toxin, and the trichoderma strain MM14-1 can be applied to the alternaria alternata and the prevention and treatment application of the alternaria alternata toxin, can overcome the problems of environmental safety, health safety and the like caused by the use of chemical pesticides, is beneficial to pollution-free production of agricultural products, and can improve the product quality. The method provides a strain for degrading the Alternaria alternata toxin more efficiently, safely and stably, and realizes the breakthrough from 'antibacterial' to 'detoxification'.

Inventors

• FAN YINGYING
• WANG YAN
• LIAN WEIJIA
• LI BINBIN
• DU JIANGTAO
• Qiu Shanmei
• LIU FENGJUAN
• CUI XING
• WANG CHENG
• HE WEIZHONG
• JIANG NA

Assignees

• 新疆维吾尔自治区农业科学院

Dates

Publication Date

20260505

Application Date

20260129

Claims (6)

1. 1. Trichoderma asperellum (Trichoderma crassum) MM14-1.
2. 2. Use of trichoderma (Trichoderma crassum) MM14-1 according to claim 1 for degradation of alternaria alternata toxin.
3. 3. Use of trichoderma (Trichoderma crassum) MM14-1 according to claim 1 for the preparation of a product for degradation of alternaria toxin.
4. 4. The use according to claim 2, wherein the alternaria toxin is tenaculum acid.
5. 5. The use according to claim 3, wherein the alternaria toxin is tenaculum acid.
6. 6. A degradation formulation of alternaria alternata, characterized in that it comprises at least one of trichoderma asperellum (Trichoderma crassum) MM14-1, a culture thereof, or an intracellular crude extract thereof according to claim 1.

Description

Bacterial strain for degrading alternaria alternata toxin and application thereof Technical Field The application relates to the technical field of microorganisms, in particular to a strain for degrading alternaria alternata toxin and application thereof. Background Xinjiang is a golden area for processing tomatoes in China, the output of the processing tomatoes accounts for more than 80% of the total output of the tomatoes in China, but with the continuous expansion of the planting scale of the processing tomatoes in Xinjiang and the popularization of a frameless cultivation mode, the problem of insufficient ventilation and light transmittance in fields is increasingly prominent, and the frequent occurrence of tomato fungal diseases is caused. The tomato fruits are often infected by Alternaria sp after harvesting, so that black and sunken spots appear on the tomato fruits, the tomato yield is seriously influenced, and simultaneously, alternaria toxins such as Alternaria Methyl Ether (AME), alternaria phenol (AOH), tenasconic acid (TeA), tenatoxin (TEN) and the like can be accumulated in the tomato bodies, so that the tomato fruits are harmful to human health, wherein the TeA toxin is a mycotoxin with cytotoxicity and genetic toxicity, and the detection rate and the detection content of the TeA toxin in tomato products and grains are all the first of various Alternaria toxins. The novel agricultural product can directly cause rot and economic loss of agricultural products, can form potential acute and chronic health threats to consumers by polluting food chains, has cytotoxicity, genotoxicity, mutagenicity, digestive system injury, liver and kidney dysfunction, immunosuppression and even potential cancerogenic risks, and is a risk factor which is of great concern in the field of food safety. At present, in terms of prevention and control technology, the traditional physical methods such as heat treatment, ultraviolet radiation and the like can have a certain effect on the Alternaria alternata toxin, but the effect is influenced by various factors and possibly influences the quality of food. Chemical control measures for Alternaria sp at home and abroad still depend on chemical pesticides highly, but the problems of drug resistance and pesticide residues are increasingly prominent. Therefore, biological control is a research hotspot due to its environmentally friendly and sustainable properties. The microbial toxin degrading method can specifically target the toxic group of mycotoxin and convert the toxic group into low-toxicity or non-toxic products, and has important significance for realizing the safety and high-value utilization of polluted agricultural products. However, the field of biodegradation is still in a preliminary exploration stage at present, and the research basis is weak. In recent years, few researches are carried out by taking the Alternatoxin as the only carbon source to successfully isolate the strain with degradation capability, but systematic work is still very limited, especially the researches on degradation key functional enzymes and molecular mechanisms are very lacking, and the degradation effect is not clear, so that the conversion of the technology to practical application is severely restricted. Disclosure of Invention The embodiment of the application provides a strain for degrading the alternaria toxin and application thereof, and the trichoderma strain is applied to the degradation of the alternaria toxin, so that degradation bacteria directly act on toxin molecules to decompose the toxin molecules into low-toxicity or non-toxic products, and compared with the traditional biodegradation method, the strain is more efficient, safer and more stable, and the technical problems of safety of agricultural products and poor degradation effect of the alternaria toxin in the prior art are solved. Realizes the large-scale preparation of the active crude extract from the high-efficiency strain, identifies the key active components of the active crude extract, develops a biocontrol agent suitable for agricultural product treatment, and realizes the breakthrough from 'fungus prevention' to 'detoxification'. In a first aspect, embodiments of the present application provide a Trichoderma (Trichoderma crassum) MM14-1. In one possible implementation, the use of Trichoderma (Trichoderma crassum) MM14-1 for degrading Alternatoxin. In one possible implementation, the use of trichoderma asperellum (Trichoderma crassum) MM14-1 for the preparation of a product that degrades alternaria alternata toxin. In one possible implementation, the alternaria toxin is tenaculum acid. Still further, the present application provides a degradation preparation of Alternaria alternata toxin, which contains at least one of Trichoderma asperellum (Trichoderma crassum) MM14-1, a culture thereof, or an intracellular crude extract thereof. One or more technical solutions provided in the embodiments of the present appli