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CN-121975636-A - Aspergillus niger capable of producing mycelium protein in high yield

CN121975636ACN 121975636 ACN121975636 ACN 121975636ACN-121975636-A

Abstract

The invention discloses aspergillus niger capable of producing mycelium protein in high yield, and belongs to the technical field of strain screening. According to the invention, the Aspergillus niger strain FS-A-25 subjected to ARTP mutagenesis is fermented to 10% corn steep liquor, so that more mycelium protein can be produced, the biomass is increased from 11.20 g/of A starting strain to 14.97 g/, the biomass is increased by 33.66%, the total amino acid content in mycelium is increased by 21.10% compared with the starting strain, and the Trp and Met contents are respectively increased by 25.00% and 18.91% compared with the starting strain. The degree of amino acid distribution balance of Aspergillus niger FS-A-25 mycelium is improved compared with that of the original strain.

Inventors

  • SUN XIULAN
  • GAO SONG
  • LI HUI
  • SUN JIADI
  • YE YONGLI
  • YUAN WEI
  • ZHANG YINZHI

Assignees

  • 江南大学
  • 合成生物学海河实验室

Dates

Publication Date
20260505
Application Date
20260202

Claims (10)

  1. 1. The Aspergillus niger FS-A-25 is characterized in that the Aspergillus niger FS-A-25 is preserved in ChinA center for type culture Collection, the preservation number is CCTCC NO: M2026200, and the preservation date is 2026, 1 month and 22 days.
  2. 2. A microbial agent comprising the Aspergillus niger FS-A-25 of claim 1.
  3. 3. The microbial agent of claim 2, wherein the microbial agent is a liquid microbial agent or a solid microbial agent.
  4. 4. The microbial agent of claim 2, wherein the number of spores of aspergillus niger FS-A-25 per gram or per milliliter of microbial agent is not less than 10 9 CFU.
  5. 5. The application of the Aspergillus niger FS-A-25 of claim 1 or the microbial agent of any of claims 2-4 in the agricultural field.
  6. 6. The use according to claim 5, characterized in that it comprises the preparation of corn steep liquor feed.
  7. 7. A method for preparing corn steep liquor feed is characterized in that Aspergillus niger FS-A-25 according to claim 1 or A microbial agent according to any one of claims 2-4 is used for inoculating corn steep liquor, fermenting, mixing with corn flakes, and drying to obtain corn steep liquor feed.
  8. 8. The method of claim 7, wherein the corn steep liquor has a solids content of 10-20%.
  9. 9. The method of claim 7, wherein the inoculum size of Aspergillus niger FS-A-25 in the corn steep liquor is 5% v/v to 15% v/v.
  10. 10. The method of claim 7, wherein the fermentation is at 25-35 ℃ for 48-96 hours.

Description

Aspergillus niger capable of producing mycelium protein in high yield Technical Field The invention relates to aspergillus niger capable of producing mycelium protein in high yield, and belongs to the technical field of strain screening. Background Corn steep liquor is a byproduct rich in nutrients such as protein, amino acid and the like generated in the corn deep processing process, has high utilization value, and is particularly suitable for being used as a high-quality protein source of animal feed. China is the second largest maize producing country and consumer country worldwide, processing over 1.2 million tons of maize in 2024 years, estimated to produce about 5 million tons of maize pulp. Typically, corn steep liquor is sprayed onto corn husks to produce a sprayed corn husks. About 40% of the corn steep liquor solids are protein, but lack tryptophan, methionine, etc., resulting in malnutrition. Aspergillus niger (Aspergillus niger) is used as a filamentous fungus widely applied in the fermentation industry, can produce various enzymes and has strong lignocellulose degrading capacity, and fermentation products are subjected to strict safety evaluation, so that the aspergillus niger is nontoxic and harmless, and mycelium proteins can be produced by utilizing the fermentation of corn steep liquor. The amino acid distribution of the Aspergillus niger mycelium protein is more balanced, and the Aspergillus niger mycelium protein is more suitable for being used as feed or microbial protein. But its low biomass in corn steep liquor limits its conversion to mycelium proteins using corn steep liquor. Therefore, developing an Aspergillus niger strain with high mycelium protein yield is of great importance for improving the utility value of corn steep liquor. In the prior document, the symbiotic fermentation characteristic of biological bacterial manure produced by bioconverting corn steep liquor is disclosed that aspergillus niger is cultivated by taking corn steep liquor with the concentration of 200g/L as a culture medium, but the solid content of the corn steep liquor in the culture medium still reaches 6.6%, while in the prior patent CN120944713A applied by the inventor, the aspergillus niger is cultivated by using corn steep liquor with higher solid content, but the inventor finds that the amino acid content in the mycelium of the aspergillus niger reaches the bottom when the aspergillus niger and the corn steep liquor are actually used for preparing feed, the fermentation condition of the feed is not ideal and a large amount of toxins exist. Therefore, the Aspergillus niger which has various performances and is more beneficial to fermenting corn steep liquor is screened to have extremely high practical value and economic value. Disclosure of Invention In order to solve the problems, the invention screens and obtains a strain which has the effects of inhibiting mixed bacteria and efficiently producing enzyme on the basis of tolerating high corn steep liquor concentration. The first object of the invention is to provide an Aspergillus niger FS-A-25, wherein the Aspergillus niger FS-A-25 is preserved in ChinA center for type culture Collection, the preservation number is CCTCC NO: M2026200, and the preservation date is 2026, 1 month and 22 days. The second object of the present invention is to provide A microbial agent, which is characterized in that the microbial agent contains the above Aspergillus niger FS-A-25. In one embodiment, the microbial agent is a liquid agent or a solid agent. In one embodiment, the number of spores of Aspergillus niger FS-A-25 per gram or per milliliter of microbial agent is not less than 10 9 CFU. The third object of the invention is to provide the application of the Aspergillus niger FS-A-25 or any of the microbial agents in the agricultural field. In one embodiment, the use comprises preparing a corn steep liquor feed. The fourth object of the invention is to provide A method for preparing corn steep liquor feed, which is characterized in that the aspergillus niger FS-A-25 or any of the microbial agents is used for inoculating corn steep liquor, and the corn steep liquor feed is obtained by mixing the corn steep liquor feed with corn chips after fermentation and drying. In one embodiment, the solids content of the corn steep liquor is 10-20%. In one embodiment, the inoculum size of Aspergillus niger FS-A-25 in the corn steep liquor is 5% v/v to 15% v/v. In one embodiment, the corn steep liquor is mixed with corn flakes according to a mass ratio of 3-6:1 after fermentation. In one embodiment, the fermentation is at 25-35 ℃ for 48-96 hours. The beneficial effects of the invention are that According to the invention, the Aspergillus niger strain FS-A-25 is obtained by mutagenesis, wherein the Aspergillus niger strain FS-A-25 subjected to ARTP mutagenesis is fermented to produce more mycelium protein, the biomass is improved from 11.20 g/of A starting strain to 14.97 g/, the bi