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CN-121975637-A - Aspergillus niger strain and application thereof in fermentation of corn steep liquor

CN121975637ACN 121975637 ACN121975637 ACN 121975637ACN-121975637-A

Abstract

The invention discloses aspergillus niger and application thereof in fermented corn steep liquor, and belongs to the technical field of strain screening. The invention provides A mutant aspergillus niger FS-A-24, which has good tolerance to high-concentration corn steep liquor, shortens the growth time on A20% corn steep liquor flat plate, shortens the spore-full time from 5 d of A starting strain to 4 d, advances the time point of obviously improving biomass in A10% corn steep liquor medium from about 72h of the starting strain to about 60 h, improves the total amino acid content of mycelium by 20.11% during fermentation of 72h, and improves the content of various amino acids.

Inventors

  • SUN XIULAN
  • GAO SONG
  • LI HUI
  • SUN JIADI
  • REN YICHANG
  • JI JIAN
  • ZHANG YINZHI
  • HUANG ZIYANG

Assignees

  • 江南大学
  • 江苏集萃未来食品技术研究所有限公司

Dates

Publication Date
20260505
Application Date
20260202

Claims (10)

  1. 1. The Aspergillus niger FS-A-24 is characterized in that the Aspergillus niger FS-A-24 is preserved in ChinA center for type culture Collection, the preservation number is CCTCC NO: M2026199, and the preservation date is 2026, 1 month and 22 days.
  2. 2. A microbial agent comprising the Aspergillus niger FS-A-24 of claim 1.
  3. 3. The microbial agent of claim 2, wherein the microbial agent is a liquid microbial agent or a solid microbial agent.
  4. 4. The microbial agent of claim 2, wherein the number of spores of aspergillus niger FS-A-24 per gram or per milliliter of microbial agent is not less than 10 9 CFU.
  5. 5. The application of the Aspergillus niger FS-A-24 of claim 1 or the microbial agent of any of claims 2-4 in the agricultural field.
  6. 6. The use according to claim 5, characterized in that it comprises the preparation of corn steep liquor feed.
  7. 7. A method for preparing corn steep liquor feed is characterized in that Aspergillus niger FS-A-24 as claimed in claim 1 or A microbial agent as claimed in any one of claims 2-4 is used for inoculating corn steep liquor, fermenting, mixing with corn flakes, and drying to obtain corn steep liquor feed.
  8. 8. The method of claim 7, wherein the corn steep liquor has a solids content of 10-20%.
  9. 9. The method of claim 7, wherein the inoculum size of Aspergillus niger FS-A-24 in the corn steep liquor is 5% v/v to 15% v/v.
  10. 10. The method of claim 7, wherein the fermentation is at 25-35 ℃ for 48-96 hours.

Description

Aspergillus niger strain and application thereof in fermentation of corn steep liquor Technical Field The invention relates to aspergillus niger and application thereof in fermented corn steep liquor, belonging to the technical field of strain screening. Background Aspergillus niger (Aspergillus niger) is a generally accepted and safe (GRAS) filamentous fungus, can secrete more than 30 extracellular enzymes such as amylase, cellulase, pectase, protease and the like, and has wide application in biological modification of plant feed raw materials such as corn steep liquor, bean pulp, miscellaneous meal and the like, in particular in the production process of animal feed. Corn steep liquor is favored as a common fermentation substrate because of its abundant nutritional ingredients and low cost, but has the four stress characteristics of high nutrition, high salt, high penetration, high SO 2 residue, and produces significant inhibition on spore germination, hypha extension and enzyme protein synthesis of filamentous fungi. When Aspergillus niger is used in corn steep liquor to prepare feed, a series of challenges are faced in the fermentation process, including problems of poor tolerance, insufficient fermentation, contamination by mixed bacteria, and toxin production. In the prior document, the symbiotic fermentation characteristic of biological bacterial manure produced by bioconverting corn steep liquor is disclosed that Aspergillus niger is cultivated by taking corn steep liquor with the concentration of 200g/L as a culture medium, but the solid content of the corn steep liquor in the culture medium is still lower and is only 6.6%, while in the patent CN120944713A filed earlier by the inventor, the Aspergillus niger is cultivated by using corn steep liquor with higher solid content, but the inventor finds that the amino acid content in Aspergillus niger mycelia is lower and the fermentation condition of the feed is not ideal and a large amount of toxin exists when the Aspergillus niger and the corn steep liquor are actually used for preparing the feed. Therefore, the Aspergillus niger which has various performances and is more beneficial to fermenting corn steep liquor is screened to have extremely high practical value and economic value. Disclosure of Invention In order to solve the problems, the invention screens and obtains a strain which has the effects of inhibiting mixed bacteria and efficiently producing enzyme on the basis of tolerating high corn steep liquor concentration. The first object of the invention is to provide an Aspergillus niger FS-A-24, wherein the Aspergillus niger FS-A-24 is preserved in ChinA center for type culture Collection, the preservation number is CCTCC NO: M2026199, and the preservation date is 2026, 1 month and 22 days. The second object of the present invention is to provide A microbial agent comprising the above-mentioned Aspergillus niger FS-A-24. In one embodiment, the microbial agent is a liquid agent or a solid agent. In one embodiment, the number of spores of Aspergillus niger FS-A-24 per gram or per milliliter of microbial agent is not less than 10 9 CFU. The third object of the invention is to provide the application of the Aspergillus niger FS-A-24 or any of the microbial agents in the agricultural field. In one embodiment, the use comprises preparing a corn steep liquor feed. The fourth object of the invention is to provide A method for preparing corn steep liquor feed, which comprises inoculating Aspergillus niger FS-A-24 or any of the microbial agents to corn steep liquor, fermenting, mixing with corn flakes, and drying to obtain corn steep liquor feed. In one embodiment, the solids content of the corn steep liquor is 10-20%. In one embodiment, the inoculum size of Aspergillus niger FS-A-24 in the corn steep liquor is 5% v/v to 15% v/v. In one embodiment, the corn steep liquor is mixed with corn flakes according to a mass ratio of 3-6:1 after fermentation. In one embodiment, the fermentation is at 25-35 ℃ for 48-96 hours. The beneficial effects of the invention are that The invention provides A mutant Aspergillus niger FS-A-24, which has good tolerance to high-concentration corn steep liquor, shortens the growth time on A corn steep liquor flat plate of 20% (solid content), shortens the spore-growing time from 5d of A starting strain to 4 d, advances the time point of obviously improving biomass in A10% corn steep liquor medium from about 72 h of the starting strain to about 60 h, improves the total amino acid content of mycelium by 20.11% during fermentation of 72 h, and improves various amino acid contents. On the basis, the amylase activity of the FS-A-24 mycelium can reach 54.81U/g, the enzyme activity of the fermentation liquor reaches 70.47U/m, and the inhibition rate of the fermentation liquor to aspergillus flavus reaches 85.83 percent, which is obviously superior to that of FS-10. Preservation of biological materials Aspergillus niger Aspergillus nige