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CN-121975638-A - Aspergillus niger NO1 and application thereof

CN121975638ACN 121975638 ACN121975638 ACN 121975638ACN-121975638-A

Abstract

The embodiment of the invention discloses aspergillus niger NO1 and application thereof. The Aspergillus niger (Aspergillus niger) NO1 is preserved in the microorganism strain collection of Guangdong province at the year 2025, 11 and 21, and the preservation address is that the No. 100 university No. 59 building 5 in Guangzhou city martyr, and the preservation number is GDMCC No:67338. The invention takes the sediment of the Zhangzhou sea mariculture farm and the high enrichment culture sewage as samples, and obtains a strain with excellent growth performance and good utilization performance on sodium nitrite through preliminary screening and secondary screening culture. The identification proves that the strain is Aspergillus niger NO1, has high-efficiency aerobic denitrification function, has excellent degradation efficiency on nitrite in aquaculture water, has good safety, does not have adverse effect on aquaculture organisms and water environment, and has wide practical application prospect in the field of aquaculture water quality purification.

Inventors

  • LUO XIUZHEN

Assignees

  • 漳州卫生职业学院

Dates

Publication Date
20260505
Application Date
20260224

Claims (9)

  1. 1. Aspergillus niger (Aspergillus niger) NO1 is deposited in the microorganism strain collection of Guangdong province at 2025, 11 and 21, and has the deposit address of Guangzhou city martyr, no. 100, no. 59, no. GDMCC No and No. 67338.
  2. 2. A seed solution or a fungicide containing the aspergillus niger NO1 strain of claim 1.
  3. 3. The preparation method of the strain seed liquid according to claim 2, which is characterized by comprising the steps of inoculating the aspergillus niger NO1 according to claim 1 on the inclined surface of a PDA culture medium, culturing for 72 hours at 28 ℃ until spores grow on the inclined surface, eluting the spores with sterile normal saline, and adjusting the concentration of the spores to 0.5-1.0X10 7 CFU/mL to obtain the strain seed liquid.
  4. 4. The method for preparing seed liquid of bacterial strain according to claim 3, wherein the PDA culture medium comprises peeled potato 200.0g, glucose 20.0g, agar powder 14.0g, and water to 1000mL.
  5. 5. The preparation method of the microbial inoculum according to claim 2 is characterized by comprising the steps of uniformly mixing the strain seed liquid according to claim 3 with sterilized and dried wheat bran carriers, airing at room temperature, and sealing and preserving.
  6. 6. The method for preparing a microbial inoculum according to claim 5, wherein the amount of the strain seed liquid is 20-25% of the mass of the wheat bran carrier.
  7. 7. Use of the aspergillus niger NO1 of claim 1, the strain seed liquid or the microbial inoculum of claim 2 in the purification of nitrite-containing water bodies.
  8. 8. A method for purifying a nitrite-containing water body, which is characterized by comprising the steps of adding the strain seed liquid or the microbial inoculum according to claim 2 to the nitrite-containing water body and standing.
  9. 9. The method of purifying according to claim 8, wherein, The pH value of the nitrite-containing water body is 3-8; the temperature of the standing is 26-30 ℃.

Description

Aspergillus niger NO1 and application thereof Technical Field The embodiment of the invention relates to the technical field of microorganisms, in particular to Aspergillus niger NO1 and application thereof, and more particularly relates to Aspergillus niger NO1 with high-efficiency nitrite degradation activity and application thereof in water body purification. Background The aquaculture mode is changed from a rough aquaculture mode to a high-density and intensive mode, so that the aquaculture efficiency and yield are improved, but water environment pollution problems such as water eutrophication, ecological balance damage, frequent occurrence of aquatic animal diseases and the like are brought, and the healthy and sustainable development of the aquaculture industry is restricted. In the high-density and intensive cultivation process, a large amount of baits are put in to cause unutilized baits and excreta to remain in the cultivation water body, and the water quality is seriously deteriorated, wherein nitrite nitrogen (NO 2- -N) is one of key factors influencing the water quality. The combination of nitrite nitrogen and heme produces oxyheme, so that the oxygen carrying capacity of blood is reduced, heme diseases or brown diseases are caused, the immunity of the culture is reduced, the movement quantity is reduced, the bottom is dead, and huge economic loss is brought to raisers. Under the background, the fact that the content of harmful nitrogen nitrite nitrogen in the aquaculture water body is too high causes a stress effect on aquatic animals, and the lack of an efficient and practical technology on eutrophication of the nitrite nitrogen in the water body is one of the key problems of great commonality faced by the aquaculture industry at present, and is also an important factor for restricting the healthy and sustainable development of the aquaculture industry. How to efficiently remove nitrite nitrogen in water body is significant for the healthy sustainable development and water environment protection of the aquaculture industry. At present, aiming at the problem of harmful nitrogen in the culture water body, the technical means such as a chemical method, a physical and chemical method, a biological method and the like are mainly adopted. The chemical method comprises an ion exchange method, a break point chlorination method, an electrochemical treatment method and the like, is simple and convenient to operate and quick in reaction, but chemical reagents are usually required to be added, so that the cost is increased, part of chemical reagents are remained in a water body after long-term use, secondary pollution is easily caused, the method does not accord with the concept of green sustainable development, obvious limitations exist in practical application, the physical and chemical method comprises an adsorption method, a stripping method and the like, the method is suitable for treating low-concentration nitrogen-containing wastewater, the effect of treating high-concentration ammonia nitrogen wastewater is poor, the defects of high cost, easiness in secondary pollution and the like exist, the biological method converts harmful nitrogen into non-toxic substances through the conversion or degradation effect of microorganisms, the ecological safety is high, the method overcomes the defect that the secondary pollution is easily caused by the chemical method and the physical and chemical method, and the method has the advantages of simple and convenient operation, cost saving, strong environmental adaptability and the like, and becomes a research hot spot for the current nitrogen pollution treatment of aquaculture water body. The traditional biological denitrification technology is a process that harmful nitrogen is converted into nitrogen through biochemical reaction under the action of microorganisms. The process comprises three steps of (1) ammoniation, namely converting organic nitrogen of wastewater into ammoniacal nitrogen, (2) nitration, namely converting ammoniacal nitrogen into nitrite nitrogen and nitrate nitrogen under the action of aerobic bacteria, and (3) denitrification, namely converting nitrite nitrogen and nitrate nitrogen into nitrogen or nitrogen oxide under the action of anaerobic bacteria, and removing the nitrite nitrogen and the nitrate nitrogen from the culture water body. The technology can remove ammoniacal nitrogen and nitrite nitrogen, but the nitrification and denitrification are respectively carried out in an aerobic tank and an anaerobic tank, and has the defects of large occupied area, high cost, difficult management and the like, and has limitation in conflict with the high oxygen dissolution condition of aquaculture. The aerobic denitrification is a novel biological denitrification technology, and has the core that the aerobic denitrification bacteria are utilized to realize the nitrification and denitrification reaction simultaneously under the aerobic condition, so