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CN-121975640-A - Fungus strain 41233 derived from cissama and application thereof

CN121975640ACN 121975640 ACN121975640 ACN 121975640ACN-121975640-A

Abstract

The invention discloses a fungal strain 41233 derived from cissamum and application thereof, the taxonomic name of the fungal strain 41233 is Plectosphaerellaceae sp, the preservation number is GDMCC No.67505, the preservation date is 2025 and 12 months and 19 days, the fungal strain 41233 and application of crude extract thereof in preparing pathogenic bacteria inhibition products are also disclosed, the fungal strain 41233 derived from cissamum has inhibition activity on mango anthracnose and/or phytophthora capsici, and the crude extract of the fungal strain 41233 derived from cissamum has inhibition activity on various pathogenic bacteria such as anthracnose, phytophthora, fruit rot and the like, and can be used for preparing biocontrol bactericides or further excavating active natural products and providing a new green control means for plant disease prevention and control.

Inventors

  • YE YONGHAO
  • YANG XIAO
  • SONG RONGFA
  • GUO ZHIKAI
  • LU JUNYAN
  • XIONG ZIJUN
  • ZHANG SHIQING

Assignees

  • 南京农业大学三亚研究院

Dates

Publication Date
20260505
Application Date
20260408

Claims (8)

  1. 1. A fungal strain 41233 derived from cissa, wherein said fungal strain 41233 has a taxonomic designation Plectosphaerellaceae sp, a deposit number GDMCC No.67505, a deposit date 2025, 12 months and 19 days, a deposit unit being the collection of microbial strains in guangdong province, a deposit address being the building 5 of institute of microbiology, university 100, line 59 in the guangdong state, viet-xiu martyr.
  2. 2. The use of the fungal strain 41233 according to claim 1 for the preparation of a pathogenic bacteria inhibiting product, wherein the pathogenic bacteria are mango anthracnose Colletotrichum gloeosporioides and/or phytophthora capsici Phytophthora capsici.
  3. 3. A crude extract of a fungal strain 41233 from cisa, characterized in that it is obtained by fermentation and extraction using a fungal strain 41233 according to claim 1.
  4. 4. A crude extract of a fungal strain 41233 derived from cissama according to claim 3, characterised in that the crude extract of fungal strain 41233 is obtained by a process comprising the steps of: (1) Seed liquid culture, namely inoculating the fungus strain 41233 in the claim 1 to a plate containing a PDA culture medium for light-shielding culture, taking fungus blocks into the seed liquid culture medium, and culturing again in the light-shielding culture medium to obtain seed liquid; (2) Solid fermentation, namely inoculating the seed liquid obtained in the step (1) onto a rice ME solid culture medium, and carrying out light-shielding stationary culture to obtain a fermented product; (3) Extracting antibacterial active substances, namely extracting the fermented product obtained in the step (2) with ethyl acetate under the assistance of ultrasound, collecting an extracting solution, filtering, and concentrating to remove all solvents to obtain the crude extract of the cissamum-derived fungus strain 41233.
  5. 5. The crude extract of a cisco-derived fungus strain 41233 as claimed in claim 4, wherein in step (1) the fungus strain 41233 is inoculated onto a plate containing PDA medium and cultivated at 28-32℃for 7 days in the absence of light, and in step (1) seed liquid is obtained after further cultivation at 28-32℃for 7 days in the absence of light at a rotational speed of 180-200 rpm.
  6. 6. The crude extract of a fungal strain 41233 derived from cissama as claimed in claim 4, wherein the seed liquid is inoculated in an amount of 4-6% of the total mass of the rice ME solid medium in the step (2), and the fermented product is obtained after the fermentation is carried out in a light-proof and static culture at 28-32 ℃ for 30 days in the step (2).
  7. 7. The crude extract of a cisco-derived fungal strain 41233 according to claim 4, wherein in step (3) the ferment is in equal volume with the ethyl acetate.
  8. 8. The use of a crude extract of a cissamum-derived fungus strain 41233 according to any one of claims 3-7 for the preparation of a pathogenic bacteria inhibition product, said pathogenic bacteria being one or a combination of several of phytophthora camphorata Phytophthora cinnamomi, phytophthora nicotianae Phytophthora nicotianae, phytophthora durian YCG1 Phytophthora palmivora, colletotrichum banana Colletotrichum musae, colletotrichum mango Colletotrichum gloeosporioides, colletotrichum rubber GX1655 Colletotrichum australisinense, colletotrichum rubber HBCg01 Colletotrichum gloeosporioides, putrescence coco Thielaviopsis paradoxa and putrescence passion Diaporthe passifloricola.

Description

Fungus strain 41233 derived from cissama and application thereof Technical Field The invention belongs to the technical field of microorganisms, and particularly relates to a cissand-derived fungus strain 41233 and application thereof. Background Islands are an important component of the marine ecosystem, and their biodiversity is relatively simple in constitution, and thus are a good quality natural laboratory for studying biological evolution. In recent years, research on island biodiversity has been focused on plants, but little research on microorganisms is related, and particularly fungus communities in the ecological system are likely to have completely different evolution paths, so that abundant resources can be provided for novel fungus excavation. Fungi serve as an important source of natural active substances, secondary metabolites provide abundant lead compounds for drug development, and unique novel fungi on islands participating in community co-evolution can have distinctive metabolic strategies and pathways, so that secondary metabolites with novel frameworks are synthesized. With the development of high throughput sequencing technology, many unknown taxonomies have not been discovered yet, demonstrating the considerable importance of deep mining and utilization of such resources. The west sand islands are important tropical multielement ecological systems in China, integrate various ecological compositions such as islands, coral reefs, mangroves, seaweed beds and the like, and contain abundant and various biological resources. The related studies of endophytic microorganisms like corals, mangroves and the like have been quite numerous, and the reported novel frameworks and antibacterial and antitumor active substances further illustrate the application potential, but the studies of island-in-sea coastal areas are relatively few. Most of the islands and coastal areas of the cilium islands are still in pioneer stage, and most of the soil matrixes are sandy soil mixed with coral particles, so that only simple salt-tolerant drought-tolerant pioneer plants grow. The microbiota of the root system of the microbial community falls under the condition stress of high temperature, high salt and strong sunlight, and a unique metabolic mechanism is evolved to assist the microbial community to better occupy the ecological niche of the needed habitat, so that a series of novel active substances are likely to be produced in the process, and the microbial community is a high-quality microbial resource with research potential. Hainan is an important natural rubber, tropical fruits, winter melon and vegetable production base and a crop seed south propagation base in China, but the ecological environment of high temperature and high humidity in the island makes the whole transportation link of the tropical economic crops and the melon and vegetable preserved from planting to picking extremely vulnerable to various agricultural pathogenic microorganisms, and seriously influences the yield and quality of crops. Pathogenic bacteria such as anthrax (Colletotrichum), fusarium (Fusarium) and Phytophthora (Phytophthora) cause great losses to various economic crops such as melons, vegetables and fruits, and the development of related economic industries is seriously jeopardized. Although the most effective means for disease control is still chemical pesticides at present, the long-term application of the main stream pesticides is accompanied with the increase of pathogen resistance, so that the dosage and the frequency are continuously increased, the cost is increased, the disease development cannot be effectively restrained, and meanwhile, the concept of green sustainable development of Hainan is not met. Therefore, the searching of a novel biological source natural pesticide which has small toxic and side effects, is safe and effective and is compatible with the environment is a main stream idea for solving the current dilemma. Disclosure of Invention The invention aims to provide a fungal strain 41233 derived from cissand, wherein the fungal strain 41233 has inhibitory activity on various agricultural pathogenic bacteria. The invention also aims to provide application of the fungus strain 41233 in preparation of pathogenic bacteria inhibition products. It is a final object of the present invention to provide a crude extract of a fungal strain 41233 of cisa origin and its use in the preparation of a pathogen inhibition product. The first object of the present invention can be achieved by a fungal strain 41233 derived from cissa, the fungal strain 41233 having a taxonomic designation Plectosphaerellaceae sp, a deposit number GDMCC No.67505, a deposit date of 2025, 12 months and 19 days, a deposit unit of the microorganism strain deposit center in Guangdong province, a deposit address of building 5 of No. 59 of the university 100 in the View martyr area of Guangzhou province, and a microbiological institute of the unive