CN-121975642-A - Apiotrichum loubieri and application thereof

Abstract

The invention discloses Apiotrichum loubieri and application thereof. Relates to the technical field of fermentation. The invention provides a specific strain and application thereof in reconstituted tobacco. The tobacco leaf extract obtained by fermenting the strain not only remarkably improves the content of esters such as 2, 4-trimethyl-1, 3-pentanediol monoisobutyrate, dimethyl phthalate, tributyl phosphate, dihydro kiwi lactone, methyl palmitate and the like, but also newly generates 9 novel ester substances with characteristic aroma such as aromatic odor, fruit aroma, flower aroma and the like, and 4 phenol aroma components are newly added. The overall fragrance style is significantly enhanced. The aroma-producing Apiotrichum loubieri AL-4 fermented tobacco leaf enzyme extract has good aroma-producing capability, and is expected to play an important application value in the field of quality improvement of reconstituted tobacco raw materials through the combined action of enzymolysis and fermentation.

Inventors

• TIAN HAILONG
• LI XIAOHAN
• LIU ZILI
• LIU WEI
• Xing Zhenlei
• ZHANG JIASHU
• LI XIAOHUA
• MOU ZHIYUAN
• LI GANG
• GAO LIWEI

Assignees

• 山东瑞博斯烟草有限公司

Dates

Publication Date

20260505

Application Date

20250828

Claims (9)

1. 1. Apiotrichum loubieri AL-4 is characterized in that the preservation number is CGMCC 34939.
2. 2. Apiotrichum loubieri AL-4 as defined in claim 1 for use in fermentation products of tobacco leaves or tobacco waste.
3. 3. A microbial agent comprising Apiotrichum loubieri AL-4 of claim 1.
4. 4. Use of Apiotrichum loubieri AL-4 as defined in claim 1 in the manufacture of a tobacco product.
5. 5. The use according to claim 4 for fermenting tobacco leaves or tobacco waste.
6. 6. A method for improving the quality of tobacco leaves or tobacco waste by using Apiotrichum loubieri AL-4 as claimed in claim 1, comprising the steps of: (1) Preparing tobacco leaf extracting solution, which comprises adding penicillium oxalate MACX enzyme solution and Aspergillus brown Ab-10 enzyme solution into tobacco leaves or tobacco leaf waste; (2) Inoculating Apiotrichum loubieri AL-4 of claim 1 into a fermentation medium containing said tobacco leaf extract for fermentation culture.
7. 7. The method of claim 6, wherein the tobacco leaf or tobacco waste is added in an amount of 10g, the adding amount of the penicillium oxalicum MACX enzyme solution and the Aspergillus fuscus Ab-10 enzyme solution is 2mg protein/g tobacco leaf, the tobacco leaf extract is obtained by mixing the tobacco leaf extract with 30mL of sterile water and 100 μl of chloramphenicol, sealing, placing the mixture in a shaking table at 50 ℃ and 150rpm, and centrifuging the mixture after 24 hours.
8. 8. The method of claim 6, wherein the inoculation in step (2) is performed at a pH of 6 and a temperature of 28℃for a fermentation period of 24 hours.
9. 9. The method of claim 6, wherein the fermentation medium comprises 2% tobacco extract, 0.1% ammonium sulfate, 0.1% potassium dihydrogen phosphate, and 0.1% magnesium sulfate.

Description

Apiotrichum loubieri and application thereof Technical Field The invention relates to the technical field of fermentation, in particular to Apiotrichum loubieri and application thereof. Background The byproducts such as a great amount of tobacco stems, tobacco fragments, tobacco dust and the like generated in the cigarette production process can cause serious resource waste and environmental pollution if the byproducts are directly abandoned or burnt. For this purpose, it is usually recycled and reconstituted tobacco flakes are produced by recombinant processing. The reconstituted tobacco sheet is similar to natural tobacco in appearance and sensory properties, and can be applied to a tobacco set formula. Paper making is currently the mainstream technology for producing reconstituted tobacco. However, the product of the technology has the problems of heavy wood, insufficient sweet feeling, heavy miscellaneous gas, poor comfort and the like, and the quality improvement of the paper-making reconstituted tobacco is limited. Therefore, how to improve the sensory quality thereof has become a key topic of the current tobacco industry. At present, the improvement research on the problem mainly surrounds the optimization of the physicochemical processing technology and the adjustment and development of the raw material proportion. While such methods have achieved some success, their degree of improvement tends to be limited and may introduce new process complexity and cost pressures. Biocatalytic technology has been demonstrated to be effective in modifying biomass resources and improving sensory quality. In the tobacco field, the microorganism or the enzyme preparation thereof is utilized to treat tobacco leaf materials, so that multiple targets, namely degradation of macromolecular substances and adverse components, promotion of conversion and coordination of contents, improvement of the types and the contents of aroma components and realization of quality improvement and aroma enhancement, can be realized. However, the activity of the conventional strain is inhibited by nicotine, alkaloid and the like, and the microbial strain resources special for the aroma producing function of tobacco are relatively lacking. In particular, studies on Apiotrichum loubieri have been limited. Therefore, whether Apiotrichum loubieri and applications thereof can be provided, and overcoming the above technical shortcomings is a problem that needs to be solved by those skilled in the art. Disclosure of Invention In view of this, the present invention provides Apiotrichum loubieri and its application. The invention uses the discovery and application of high-efficiency aroma-producing microorganisms as a core strategy. Specifically, a yeast strain with remarkable flavoring potential is successfully separated, purified and screened from the tobacco surface, and is identified as Apiotrichum loubieri through molecular biology. In order to improve the quality and aroma quality of reconstituted tobacco, the invention creatively introduces a microbial enzyme enzymolysis treatment process, and determines the optimal scheme for preparing tobacco extract by utilizing the composite enzyme solution of Aspergillus fuscus (Aspergillus brunneoviolaceus) Ab-10 and Penicillium oxalate (Penicillium oxalicum) MCAX through systematic screening, thereby aiming at degrading biological macromolecules and fully releasing flavor precursor substances such as micromolecular saccharides. On the basis, key process parameters (such as temperature, pH and the like) of fermentation of Apiotrichum loubieri strains in tobacco leaf extract are optimized through a single-factor experiment. Experiments prove that the strain can efficiently utilize matrix components in tobacco leaf extract under the optimized fermentation condition, and rich and coordinated aroma compounds are generated. The aroma components in the fermentation product are analyzed by utilizing a gas chromatography-mass spectrometry (GC-MS) technology, and the aim of verifying the effect of the microbial fermentation technology on improving the quality of reconstituted tobacco is achieved. The research result of the invention can provide reference basis for improving the quality of reconstituted tobacco by using the biocatalysis technology. The preservation information is (Apiotrichum loubieri) AL-4, which is preserved in China general microbiological culture Collection center, with a preservation address of North Star Xili No.1 and 3 in the Korean region of Beijing, a preservation number of CGMCC No.34939, and a preservation date of 2025, 06 and 18. In order to achieve the above purpose, the present invention adopts the following technical scheme: Apiotrichum loubieri AL-4 with preservation number of CGMCC 34939. The invention also provides a fermentation product of Apiotrichum loubieri AL-4 for tobacco leaves or tobacco waste. The invention also provides a microbial inoculum containing Apiotrichum loub