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CN-121975645-A - Sporobora SY-18 strain and application thereof

CN121975645ACN 121975645 ACN121975645 ACN 121975645ACN-121975645-A

Abstract

The invention relates to the technical field of microorganisms, and particularly discloses a Sporobora SY-18 strain and application thereof. The Sporobusta SY-18 is preserved in China Center for Type Culture Collection (CCTCC) No. M20252277 in 2025, 10 months and 21 days, and is classified and named as Sporobolomyces sp.SY-18. The rhodotorula sp SY-18 provided by the invention can obviously improve the rhodotorula sp after fermentation by using optimized culture medium components and fermentation conditions, the ratio reaches 1.6+/-0.1 g/L, and provides high-efficiency and reliable strain resources and process basis for the large-scale production of rhodotorula erythrosin.

Inventors

  • JI HAO
  • LIU XUYUAN
  • JIN JIAHUI
  • HU JIACHUN
  • YANG BIYING
  • DENG CHENGKE

Assignees

  • 温州大学

Dates

Publication Date
20260505
Application Date
20260210

Claims (8)

  1. 1. A strain of Sporobora (Sporobolomyces sp) SY-18 is characterized in that the Sporobora SY-18 is preserved in China Center for Type Culture Collection (CCTCC) with the preservation number of M20252277 at the month of 10 and 21 of 2025.
  2. 2. Use of a rhodotorula sp SY-18 according to claim 1, in the preparation of rhodotorula sp. Fermenting and culturing the Sporobora SY-18 to obtain fermentation liquor, centrifugally collecting thalli from the fermentation liquor, performing wall breaking treatment on the thalli, extracting carotenoid, and separating and purifying the carotenoid to obtain rhodotorula erythrosin; The liquid fermentation medium used for fermentation comprises a carbon source, a nitrogen source and inorganic salt, wherein the carbon source is sucrose, the nitrogen source is potato leaching powder, and the inorganic salt is CuSO 4 .
  3. 3. The use according to claim 2, wherein the concentration of sucrose in the culture medium is 20 g/L to 50 g/L.
  4. 4. The use according to claim 2, characterized in that the concentration of potato extract powder in the culture medium is 1 g/L-16 g/L.
  5. 5. The use according to claim 2, wherein the concentration of CuSO 4 in the medium is 0.1 g/L to 2 g/L.
  6. 6. The use according to claim 2, wherein the fermentation conditions comprise an initial pH of the medium of 3 to 9 and a rotation speed of 120 to 240 rpm.
  7. 7. The use according to claim 2, wherein the fermentation time is 70-74 h.
  8. 8. The use according to claim 2, wherein the fermentation temperature is 20 ℃ to 37 ℃.

Description

Sporobora SY-18 strain and application thereof Technical Field The invention relates to the technical field of microorganisms, in particular to a Sporobora SY-18 strain and application thereof. Background Rhodotorula erythrosin (torularhodin) is a lutein carotenoid, and the molecular structure of the rhodotorula erythrosin contains 1 beta-ionone ring and a long-chain carbon skeleton containing 13 conjugated double bonds, and has provitamin A activity. Natural rhodotorula rhodopsin is produced mainly from a few yeasts and is not found in bacteria, algae and plants. The rhodotorula erythrosin has the characteristics of strong polarity, bright color and the like, has better oxidation resistance than lycopene and beta-carotene, is safe and nontoxic, and therefore, gradually attracts attention. Recent researches find that rhodotorula erythrosin has wide antioxidant, antibacterial, anti-inflammatory, anti-aging and anti-tumor activities and the like, so that the rhodotorula erythrosin has wide potential application value in the fields of functional foods, animal feeds, cosmetics, medicine and health and the like. The production of rhodotorula erythrosin can be realized by a chemical synthesis method, but the molecular weight is large, the chemical structure is complex, the chemical synthesis process is complex, byproducts are easy to generate, the biological activity is poor, and the production cost is high. In contrast, the fermentation method for producing the rhodotorula erythrocin by utilizing the saccharomycetes has the advantages of mild reaction conditions, simple nutrition requirement, quick production period, higher stability and bioactivity of the product, no food safety risk, easy acceptance by consumers and the like, so the rhodotorula erythrocin is a preferred way for realizing the large-scale production of the rhodotorula erythrocin. At present, the rhodotorula erythrosin produced by a microbial way has extremely low yield and small carotenoid proportion, and can not meet the requirement of large-scale production, so that a new strain for high-yield rhodotorula erythrosin is necessary to be mined. Disclosure of Invention The invention provides a rhodotorula sp SY-18 and application thereof for digging a new strain for high-yield rhodotorula sp. The rhodotorula sp SY-18 provided by the invention can obviously improve the rhodotorula sp after fermentation by using optimized culture medium components and fermentation conditions, the ratio reaches 1.6+/-0.1 g/L, and provides high-efficiency and reliable strain resources and process basis for the large-scale production of rhodotorula erythrosin. The invention provides a strain of Sporobora (Sporobolomycessp) SY-18, wherein the Sporobora SY-18 is preserved in China Center for Type Culture Collection (CCTCC) with the preservation number of M20252277 in the year 2025 and the month 10 and 21. The rhodotorula sp SY-18 provided by the invention can obviously improve the rhodotorula sp after fermentation by using optimized culture medium components and fermentation conditions, the ratio reaches 1.6+/-0.1 g/L, and provides high-efficiency and reliable strain resources and process basis for the large-scale production of rhodotorula erythrosin. The invention also provides an application of the Sporobora SY-18 in preparing rhodotorula rhodozyma red pigment, which comprises the following steps: fermenting and culturing the Sporobora SY-18 to obtain fermentation liquor, centrifugally collecting thalli from the fermentation liquor, performing wall breaking treatment on the thalli, extracting carotenoid, and separating and purifying the carotenoid to obtain rhodotorula erythrosin; The liquid fermentation medium used for fermentation comprises a carbon source, a nitrogen source and inorganic salt, wherein the carbon source is sucrose, the nitrogen source is potato leaching powder, and the inorganic salt package is CuSO 4. Further, the concentration of the sucrose in the culture medium is 20 g/L-50 g/L. Further, the concentration of the potato leaching powder in the culture medium is 1 g/L-16 g/L. Further, the concentration of the CuSO 4 in the culture medium is 0.1-g/L to 2-g/L. Further, the fermentation conditions comprise an initial pH value of the culture medium of 3-9 and a rotating speed of 120-240 rpm. Further, the fermentation time is 70-74 h. Further, the fermentation temperature is 20-37 ℃. Compared with the prior art, the invention has the beneficial effects that: The rhodotorula sp SY-18 for high-yield rhodotorula has the rhodotorula sp production of up to 1.6+/-0.1 g/L. The cost of the potato extract liquid used as the only carbon source and the only nitrogen source in the fermentation method is extremely low, the low temperature can increase the yield of rhodotorula erythrocin, and the rhodotorula erythrocin can be produced in low-temperature seasons or low-temperature environments so as to meet the market demand. Description of biological Material preserva