CN-121975650-A - Horse-derived anaerobic strain capable of improving exercise capacity, screening method and application thereof

Abstract

The invention discloses a horse-derived anaerobic strain for improving exercise capacity, a screening method and application thereof, and relates to the field of microorganisms. The invention discloses a method for screening equine anaerobic strains, which is characterized in that the equine anaerobic strains for improving the exercise capacity are preserved in China general microbiological culture Collection center (China general microbiological culture Collection center), the collection address is North Star Xiyu No. 1, no. 3, the collection date is 2023, 09 and 19, the collection number is CGMCC No.28469, and the classified and named enterococcus faecalis Enterococcus faecalis. The horse-derived anaerobic strain for improving the exercise capacity, the screening method and the application thereof have important guiding significance for developing the probiotic bacteria of racing horses and the additive of microbial feeds.

Inventors

• NI WEI
• HU SHENGWEI
• YAO RUI
• LIU XIA

Assignees

• 石河子大学

Dates

Publication Date

20260505

Application Date

20231122

Claims (8)

1. 1. A horse-derived anaerobic strain capable of improving exercise capacity is characterized by being preserved in China general microbiological culture Collection center, and has a preservation address of North Star Xiyu No. 1, 3 in the Korean region of Beijing, a preservation date of 2023, 09 months and 19 days, a preservation number of CGMCC No.28469 and a classification name of enterococcus faecalis Enterococcus faecalis.
2. 2. The horse-derived anaerobic strain for improving exercise ability according to claim 1, wherein the 16S rDNA sequence of the horse-derived anaerobic strain is shown as SEQ ID NO. 1.
3. 3. A method for screening a horse-derived anaerobic strain for improving exercise ability according to claim 1 or 2, comprising the steps of: a. separating strains; b. Identifying strains; c. Measuring acid production capacity of the strain; d. strain physiological and biochemical measurement; e. verify its function at the individual level.
4. 4. A method for screening a horse-derived anaerobic strain for improving exercise ability according to claim 3, wherein: the source of the strain isolation in the step a is horse racing feces.
5. 5. A method of screening a sports-enhancing strain of anaerobic equine origin according to claim 3, wherein said strain identification in step b comprises conventional phenotypic identification and 16S rDNA molecular level identification.
6. 6. The method of claim 3, wherein the culture medium for measuring the acid production capacity of the strain in the step c comprises bromomethylphenol green screening culture medium.
7. 7. Use of an equine anaerobic strain according to claim 1 for improving muscle mass and enhancing endurance exercise.
8. 8. Use of the equine anaerobic strain according to claim 1 for the preparation of probiotics and microbial feed additives.

Description

Horse-derived anaerobic strain capable of improving exercise capacity, screening method and application thereof The horse source anaerobic strain, K3-E10 strain is preserved in China general microbiological culture Collection center (China general microbiological culture Collection center), and has a preservation address of North Star Xiyu No.1, 3 in the Korean region of Beijing, a preservation date of 2023, 09 and 19 days, a preservation number of CGMCC No.28469, and a classification name of enterococcus faecalis Enterococcus faecalis. Technical Field The invention relates to the technical field of microorganisms, in particular to a horse-derived anaerobic strain for improving exercise capacity, a screening method and application thereof. Background Along with the acceleration of the world industrialization process, the horses gradually leave the meat livestock and the in-service lines, and the modern horse industry is a novel industry, and integrates culture, sports, competition and leisure. The horse culture is rich, including horse racing, horse art, horse racing, horse raising, horse service industry, etc. [ Li Zhiping. International horse industry development experience and enlightenment [ J ]. World agriculture, 2020, (02): 98-104+14 ]. However, the modern horse racing industry in our country is in a rapid development stage. Horse racing comprises speed racing, barrier racing, martial arts racing, endurance racing, etc. [ Bai Yan, guo Xiaochuan. Mechanism and mode of fusion development of inner Mongolia modern horse industry [ J ]. North economy, 2022, (02): 38-41 ]. Compared with general racing horses, top racing horses are excellent in various aspects such as speed, endurance and the like, and modern scientific researches show that the reasons for the differences are the result of the combined actions of various factors. Among them, studies have found that certain types of intestinal bacteria can activate nerves in the intestinal tract, thereby promoting the desire for exercise [DohnalováL,Lundgren P,Carty J R E,et al.A microbiome-dependent gut–brain pathway regulates motivation for exercise[J].Nature,2022:1-9.]. these findings have now prompted researchers to search for ways to increase the diversity and richness of beneficial bacteria in the intestinal tract, and have achieved better exercise capacity and faster recovery capacity. Researchers at the university of haverso compared intestinal microorganisms before and after the competition of boston marathon athletes and found that the number of a bacterium required for human lactic acid decomposition was increased. This increase in specific bacteria is a reflection of the increased lactic acid levels in the body [ Torrice M.A conversation with jonathan scheiman [ J ].2017 ]. Our team previously performed a macro-genome sequencing data analysis on a sample of the gut of 242 horses, found sports performance-related bacteria Lachnospiraceae, oscillospiraceae and Ruminococcus in the racehorse, which were enriched in the microbiome of human athletes. These bacteria that indicate motor performance-related may be conserved [Li C,Li X,Guo R,et al.Expanded catalogue of metagenome-assembled genomes reveals resistome characteristics and athletic performance-associated microbes in horse[J].Microbiome,2023,11(1):7.]. in at least some animals and humans as further studies have found that as the microorganisms change in abundance, 15 metabolites including Short Chain Fatty Acids (SCFAs) are positively correlated [Huang,Li,et al."Hypoxia improves endurance performance by enhancing short chain fatty acids production via gut microbiota remodeling."Frontiers in Microbiology 12(2022):4375.]. as further studies of fecal metabolites and microbiota before and after the half-way marathon competition by 16 amateurs have shown that specific movements may promote specific microbial ecosystems and thus metabolic changes. Some scientists are further motivated to believe that the mining of these microorganisms or their metabolites may help us provide game advantages. In recent years, the microbial preparation as an emerging industry brings great social benefit and economic benefit, and has wide development prospect in feed processing. Microbial agents are important in horse breeding and competition training, and the combination of microbial agents and feeds is becoming more and more popular .[PLANCADE S,CLARK A,PHILIPPE C,et al.Unraveling the effects of the gut microbiota composition and function on horse endurance physiology[J].Sci Rep,2019,9(1):9620.]. in endurance and expressive force, so that screening microbial agents capable of improving horse racing performance is becoming a hot spot of current research. The invention separates the microorganism related to producing short chain fatty acid from the fresh racehorse manure, carries out species identification on the separated bacteria through 16S rRNA sequencing, determines physicochemical properties such as acid and alkali