CN-121975653-A - High-temperature-resistant acid-resistant multifunctional composite microbial agent and application thereof in silage

Abstract

The invention discloses a high-temperature-resistant acid-resistant multifunctional composite microbial agent and application thereof in silage, and belongs to the technical field of agricultural microorganisms. The microbial inoculum is prepared by compounding lactobacillus CYM6 with the preservation number of CGMCC No.36209, bacillus CYC6 with the preservation number of CGMCC No.36210 and Wikipedia yeast CYY6 with the preservation number of CGMCC No.35997 according to the ratio of 8:1:1. The invention utilizes lactic acid bacteria CYM6 to rapidly produce acid to reduce pH value and inhibit putrefying bacteria through the synergistic effect of three bacteria, utilizes cellulose degrading bacteria CYC6 to degrade cellulose to improve palatability and digestibility, utilizes saccharomycete CYY6 to synthesize mycoprotein and improves the crude protein content of the feed. The microbial inoculum can quickly become dominant bacteria in an external high-temperature environment, effectively inhibit spoilage microorganisms, reduce silage pH value, degrade cellulose content of forage grass raw materials, reduce dry matter loss and protein degradation, remarkably improve fermentation quality and nutritive value of hybrid pennisetum silage, and provide effective technical support for solving the problem of high-quality silage production in southern areas.

Inventors

• YAO BO
• ZHU RUIFEN
• CHEN JISHAN
• LIU CHANG
• FAN YAN
• GAO LIFANG
• XU YUANDONG

Assignees

• 重庆市畜牧科学院

Dates

Publication Date

20260505

Application Date

20251221

Claims (8)

1. 1. The multifunctional composite microbial agent is characterized by being compounded by the following strains: (1) Lactobacillus (Lacticaseibacillus) strain CYM6 with a preservation number of CGMCC No.36209; (2) Bacillus strain CYC6 with preservation number of CGMCC No.36210; (3) The preservation number of the Wikipedia yeast (Wickerhamomyces) strain CYY6 is CGMCC No.35997; wherein the ratio of viable bacteria numbers of the lactobacillus CYM6, the bacillus CYC6 and the Wikipedia yeast CYY6 is 8:1:1.
2. 2. The composite microbial agent according to claim 1, wherein the optimal medium for lactobacillus CYM6 is MRS liquid medium, and the lactobacillus CYM6 is cultured at 37 ℃ for 24 hours to achieve a logarithmic growth phase.
3. 3. The composite microbial agent according to claim 1, wherein the bacillus CYC6 optimal medium is an LB liquid medium, and the bacillus CYC6 is cultured at 37 ℃ for 24 hours to reach a logarithmic growth phase.
4. 4. The composite microbial agent according to claim 1, wherein the optimal culture medium of the Vickers yeast CYY6 is YPD liquid culture medium, and the Vickers yeast CYY6 is cultured for 24 hours at a temperature of 30 ℃ to reach a logarithmic growth phase.
5. 5. The use of the composite microbial agent of claims 1-4 in preparing silage under high temperature and high humidity environment.
6. 6. A method for preparing silage by using the composite microbial agent of claim 1, comprising the following steps: s1, providing silage raw materials; S2, inoculating the composite silage microbial inoculum of claim 1 into the silage raw material; S3, sealing and fermenting the inoculated silage raw material; Wherein the inoculation amount of the compound microbial agent is 1X 10 7 CFU/g fresh weight of feed.
7. 7. The method according to claim 6, wherein the silage is obtained by interplanting hybrid pennisetum with croton at a ratio of 6:4, cutting, and pulverizing.
8. 8. The method of claim 6, wherein the fermentation conditions are fermentation in a 40 ℃ incubator with 85% relative humidity for 45 days.

Description

High-temperature-resistant acid-resistant multifunctional composite microbial agent and application thereof in silage Technical Field The invention belongs to the technical field of agricultural microorganisms, and particularly relates to a high-temperature-resistant acid-resistant multifunctional composite microbial agent and application thereof in silage. Background Silage is a basic stone developed in modern animal husbandry, and soluble carbohydrates in fresh forage grass are converted into organic acids through anaerobic fermentation of microorganisms such as lactobacillus, and the pH value is reduced, so that the growth of spoilage bacteria is inhibited, and the nutritional value of the forage grass is preserved for a long time. In the southern areas of China, particularly hilly mountains such as Chongqing, the hybrid pennisetum has become a main cultivated forage grass variety due to the characteristics of land resources and high-yield and high-quality warm-season forage grass. However, the region belongs to a typical subtropical monsoon humid climate, is lengthy in summer, and has the remarkable climate characteristics of high temperature and high humidity. The climate condition makes the traditional field airing preparation of hay almost impossible, and the hay is easy to mould and deteriorate in the airing process due to frequent rainwater and high air humidity, so that the nutrition loss is serious. Therefore, silage, especially wrapping silage which is convenient for storage and transportation, becomes the most main technical approach for guaranteeing the annual high-quality forage grass stable supply of the grassland animal husbandry in the area. However, it is this high temperature and high humidity environment that presents a serious challenge to the successful silage of hybrid pennisetum. Firstly, the high-temperature and high-humidity environment promotes the respiration of the hybrid pennisetum after the field mowing to be vigorous, and the surface of the hybrid pennisetum has a large number of putrefying bacteria (such as mould, saccharomycetes and butyric acid bacteria) and strong activity. If an acidic environment cannot be quickly created and the dominant position of lactic acid bacteria is established in the early stage of silage fermentation, putrefying bacteria can be greatly propagated, so that silage is heated and putrefied, the storage yield is low, and a large amount of nutrient substances are lost. Secondly, the hybrid pennisetum has the characteristics of high water content, high buffering energy value and relatively low content of soluble carbohydrates, which further increases the difficulty of quickly and efficiently starting lactic acid fermentation. Currently, fermentation processes are often guided and enhanced in production by the addition of commercial silage inoculants. However, the commercial silage additive has poor effect. Because the temperature inside the silage pit or wrap is very easily above 45 ℃ at the beginning of fermentation when silaging in summer in Chongqing. Under the high-temperature environment, the activity of the conventional microbial inoculum is seriously inhibited, the growth and propagation are slow, the rapid acid production can not be realized, and the effect is greatly reduced or even completely disabled. Meanwhile, as silage progresses, acidity in a material pile gradually accumulates, and the tolerance of some strains to a low-pH environment is insufficient, so that the activity in the middle and later stages of silage is reduced, secondary fermentation can be caused, and the long-term stability of silage is also influenced. In conclusion, the special climate of high temperature and high humidity in Chongqing area and the raw material characteristics of the hybrid pennisetum are mutually overlapped, so that a very harsh application environment for the silage microbial inoculum is formed. The lack of a indigenous and special silage microbial inoculum which can quickly start fermentation under high temperature and keep activity in a high acidity environment is a core technical bottleneck which leads to unstable silage quality of hybrid pennisetum in the area. Disclosure of Invention The invention aims to provide a multifunctional composite microbial agent with high temperature resistance and acid resistance and application thereof in silage. The microbial inoculum is compounded by high-temperature-resistant acid-resistant lactobacillus, cellulose degrading bacteria and saccharomycetes, and the quality of silage in high-temperature and high-humidity areas is improved jointly through the synergistic effect of the high-temperature-resistant acid-resistant lactobacillus, cellulose degrading bacteria and saccharomycetes. The high-temperature-resistant acid-resistant lactobacillus is responsible for rapid leading fermentation, pH value can be rapidly reduced, putrefying bacteria can be inhibited, cellulose degradation bacteria are responsible for d