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CN-121975656-A - Complex bacterium for degrading free gossypol in cottonseed molasses and fermentation process thereof

CN121975656ACN 121975656 ACN121975656 ACN 121975656ACN-121975656-A

Abstract

The invention belongs to the technical field of high-value utilization of agricultural product processing byproducts, and discloses a compound bacterium for degrading free gossypol in cottonseed molasses and a fermentation process thereof. The composite bacteria consist of aspergillus niger, saccharomyces cerevisiae and lactobacillus gasseri according to the volume ratio of bacterial liquid of 1:2:1, and the fermentation process parameters are that the inoculum size is 5%, the fermentation temperature is 27.8 ℃ and the fermentation time is 8.4d. The degradation rate of free gossypol in the cottonseed molasses reaches 72%, which is improved by 6% compared with that of single bacteria, and simultaneously, metabolic pathways such as ABC transport protein, vitamin absorption and the like can be regulated and controlled, the metabolite composition of the product is changed, and the nutritive value and the safety of the product are improved, so that the cottonseed molasses can be used for preparing feed additives or food raw materials.

Inventors

  • ZENG JUN
  • LEI YINFENG
  • TANG YUHAO
  • GAO HONGWEI
  • YIN JIAO
  • YU XIUQIANG
  • SUN XUEMEI
  • HE WENFENG
  • SHI JIANWEI
  • CHEN GUO

Assignees

  • 昌吉州泰昆生物蛋白科技有限公司

Dates

Publication Date
20260505
Application Date
20260122

Claims (9)

  1. 1. A compound bacterium for degrading free gossypol in cottonseed molasses is characterized by comprising aspergillus niger, saccharomyces cerevisiae and lactobacillus gasseri, wherein the volume ratio of bacterial liquid of the aspergillus niger to bacterial liquid of the saccharomyces cerevisiae to bacterial liquid of the lactobacillus gasseri is 1:1-3:1-2.
  2. 2. The composite bacterium according to claim 1, wherein the volume ratio of the bacterial liquids of the aspergillus niger, the saccharomyces cerevisiae and the lactobacillus gasseri is 1:2:1, wherein the bacterial liquid concentration of the aspergillus niger and the saccharomyces cerevisiae is 1×10 8 /mL, and the bacterial liquid concentration of the lactobacillus gasseri is 10 8 CFU/mL.
  3. 3. A fermentation process for degrading free gossypol in cottonseed molasses is characterized by comprising the following steps: inoculating the composite bacteria of claim 1 or 2 into a fermentation medium, and fermenting under the conditions that the inoculum size is 3-7%, the fermentation temperature is 25-30 ℃ and the fermentation time is 7-9 d; the fermentation medium takes cottonseed molasses as a base material and also contains nitrogen source, sodium salt, magnesium salt and phosphorus salt.
  4. 4. The fermentation process according to claim 3, wherein in the fermentation medium, the nitrogen source is ammonium sulfate, the sodium salt is sodium chloride, the magnesium salt is magnesium sulfate heptahydrate, the phosphorus salt is dipotassium hydrogen phosphate, and the addition amount of each component is 1.5-2.5 g of ammonium sulfate, 0.8-1.2 g of sodium chloride, 0.4-0.6 g of magnesium sulfate heptahydrate and 0.4-0.6 g of dipotassium hydrogen phosphate in each 1000mL of the medium.
  5. 5. A fermentation process according to claim 3, wherein the inoculum size is 4-6%.
  6. 6. A fermentation process according to claim 3, wherein the fermentation temperature is 27-28 ℃ and the fermentation time is 8-8.5 d.
  7. 7. Use of the complex bacteria of claim 1 or 2 for degrading free gossypol in cottonseed molasses.
  8. 8. Use of a complex bacterium according to claim 1 or 2 for the preparation of a feed additive.
  9. 9. The use according to claim 8, wherein the feed additive comprises a fermentation metabolite of the complex bacteria.

Description

Complex bacterium for degrading free gossypol in cottonseed molasses and fermentation process thereof Technical Field The invention belongs to the technical field of high-value utilization of agricultural product processing byproducts, and relates to a compound bacterium for degrading free gossypol in cottonseed molasses and a fermentation process thereof. Background The cottonseed molasses is a byproduct produced after cottonseed is treated by oil extraction and dephenolization processes, is rich in various nutritional ingredients such as crude protein, sucrose, raffinose, stachyose and the like, is superior to products such as soybean molasses, beet molasses and the like in composition and active ingredient content, and has good application potential as a raw material of food or feed. However, the cottonseed molasses contains high-concentration free gossypol, excessive intake of the substances can cause gastroenteritis, liver injury, reproductive dysfunction and other toxicological effects, the problem severely limits the direct development and utilization of the cottonseed molasses, and the efficient removal of the free gossypol becomes a key precondition for realizing the safe recycling of the cottonseed molasses. At present, in the method for removing free gossypol, the microbial degradation method has been a research hot spot because the conditions are mild and the safety is high, and the metabolic products generated by the microorganisms in the fermentation process can improve the nutrition quality of the materials. However, the related researches in the prior art are concentrated on solid materials such as cottonseed meal, cottonseed hull and the like, and the microbial degradation technology for the liquid byproduct such as cottonseed molasses is not yet systematically reported. Meanwhile, the existing microbial degradation research mostly adopts single-bacteria fermentation or simple mixed bacteria combination, has the problems of limited degradation efficiency, undefined technological parameters and the like, and is difficult to meet the requirements of industrial application on the stability and the high efficiency of the degradation effect. In addition, the prior art is focused on the removal of free gossypol, and does not pay deep attention to the regulation and control of metabolic pathways and the synchronous improvement of the nutritive value of products in the fermentation process, so that the high-value utilization of cottonseed molasses is limited. With the development of the agricultural product processing industry, the yield of cottonseed molasses is continuously increased, and unreasonable disposal of cottonseed molasses not only causes resource waste, but also can cause environmental problems. Therefore, the high-efficiency free gossypol degradation technology aiming at the cottonseed molasses is developed, fermentation process parameters are optimized, and meanwhile, the nutrition value and the safety of the product are improved by regulating and controlling the metabolic process, so that the blank of the prior art can be filled, the recycling and high-value utilization of the cottonseed molasses can be realized, the requirements of green agriculture development are met, and the method has important economic value and environmental significance. Disclosure of Invention In the prior art, the research of microbial degradation of free gossypol is concentrated on solid materials such as cottonseed meal, cottonseed hull and the like, the related technology of processing liquid byproducts of cottonseed molasses is not yet reported in a system, so that the cottonseed molasses is difficult to realize safe utilization due to high content of free gossypol, meanwhile, the existing microbial degradation scheme mainly adopts single-strain fermentation or simple mixed bacteria combination, has the problems of limited degradation efficiency (lower than 66%) of free gossypol and poor stability of degradation effect, and does not form a fermentation process parameter system which can be applied in an industrial way, in addition, the prior art is only focused on removal of the free gossypol, neglects the regulation and control of metabolic pathways and synchronous promotion of nutritive value of products in the fermentation process, and cannot meet the requirement of high-value utilization of the cottonseed molasses. Aiming at the technical blank and the deficiency, the invention needs to provide a technical proposal which can efficiently degrade the free gossypol in the cottonseed molasses, has definite process and can synchronously improve the quality of the product. Firstly, the invention provides a compound bacterium for degrading free gossypol in cottonseed molasses, which consists of aspergillus niger, saccharomyces cerevisiae and lactobacillus gasseri, wherein the volume ratio of bacterial liquid of the aspergillus niger to the saccharomyces cerevisiae to the lactobacillus gasseri is 1:1-3:1-2